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Methods in Molecular Biology (Clifton,... 2017Periodic Acid-Schiff (PAS) with diastase (PAS-D) refers to the use of the PAS stain in combination with diastase, which is an enzyme that digests the glycogen. The...
Periodic Acid-Schiff (PAS) with diastase (PAS-D) refers to the use of the PAS stain in combination with diastase, which is an enzyme that digests the glycogen. The purpose of using the PAS-D procedure is to differentiate glycogen from other PAS-positive elements in tissue samples. The PAS-D method is also used for periportal liver staining of AAT polymer inclusions that are seen in alpha-1 antitrypsin deficiency disease. Here, we describe the procedure of PAS-D staining in formalin-fixed, paraffin-embedded human liver tissues.
Topics: Amylases; Humans; Liver; Paraffin Embedding; Periodic Acid; Staining and Labeling
PubMed: 28752454
DOI: 10.1007/978-1-4939-7163-3_14 -
Analytical Biochemistry Sep 2011Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low...
Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised the periodic acid-Schiff's reagent (PAS) staining for microtiter plate assay by examining five factors: concentration and volume of periodic acid, oxidation time, volume of Schiff's reagent, and color development time. This assay requires just 25 μl of sample, utilises standardised Schiff's reagent, and has decreased assay time (140 min to completion). Seventeen monosaccharides (acidic, neutral, basic, phosphorylated, and deoxy) and four disaccharides were assessed. PAS-positive carbohydrates (amino, N-acetylamino, deoxy, and certain neutral monosaccharides, and sialic acids) responded linearly within a 10-100 nmol range approximately, which varied for each carbohydrate. The assay response for fetuin and porcine gastric mucin (PGM) was linear up to 150 μg (highest concentration tested), with no response from nonglycosylated protein. A lower response for asialofetuin was observed, but desialylated PGM preparations were similar or higher in response than their sialylated counterparts. The simplicity and low sample consumption of this method make it an excellent choice for screening or quantitation of chromatographic fractions containing carbohydrates and glycoconjugates, especially in the case of mucins.
Topics: Carbohydrate Conformation; Carbohydrates; Colorimetry; Hydrogen-Ion Concentration; Oxidation-Reduction; Periodic Acid; Rosaniline Dyes; Stereoisomerism
PubMed: 21620792
DOI: 10.1016/j.ab.2011.05.006 -
Journal of Cutaneous Pathology Jun 2019Periodic acid-Shiff (PAS) stain may help to diagnose fungal infection in biopsies from dermatoses of the palms and soles. It is questionable whether PAS stain should be...
BACKGROUND
Periodic acid-Shiff (PAS) stain may help to diagnose fungal infection in biopsies from dermatoses of the palms and soles. It is questionable whether PAS stain should be used routinely or only when tinea is suspected clinically.
METHODS
A total of 195 consecutive punch biopsies of dermatoses from the palms (90) or soles (105) were stained with PAS, regardless of the clinical differential diagnosis.
RESULTS
PAS stain showed fungi in the corneal layer of 6 (3%) of the 195 biopsies. Tinea was included in the differential diagnosis in 48 cases, of which 3 (6%) were PAS positive. PAS stain was also positive in 3 (2%) of 147 cases in which tinea was not suspected clinically. All 6 PAS-positive cases were detected in reaction patterns not readily classified as particular diagnostic entities: non-inflammatory keratoderma (2, 11%), chronic lichenified dermatitis (2, 6%), spongiotic psoriasiform dermatitis (1, 2%), and spongiotic dermatitis (1, 4%).
CONCLUSIONS
There is a low concordance rate between the clinical suspicion and the actual demonstration of fungi by PAS stain in dermatoses of the palms and soles. Routine PAS stains in non-suspected cases have a relatively low yield, which may be improved by limiting the PAS stain to reaction patterns not readily classified as particular diagnostic entities.
Topics: Biopsy; Humans; Periodic Acid; Periodic Acid-Schiff Reaction; Skin Diseases; Tinea
PubMed: 30843246
DOI: 10.1111/cup.13451 -
The American Journal of Pathology May 1948
Topics: Humans; Kidney; Periodic Acid
PubMed: 18859363
DOI: No ID Found -
Journal of Bacteriology Oct 1950
Topics: Animals; Coloring Agents; Fungi; Humans; Periodic Acid; Staining and Labeling; Tissues
PubMed: 14784469
DOI: 10.1128/jb.60.4.415-421.1950 -
International Journal of Biological... Sep 2019Modification of Hyaluronic acid (HA) with Sodium metaperiodate (NaIO) results in the formation of oxidized HA (OHA). OHA containing multiple aldehyde groups can easily... (Review)
Review
Modification of Hyaluronic acid (HA) with Sodium metaperiodate (NaIO) results in the formation of oxidized HA (OHA). OHA containing multiple aldehyde groups can easily react with the materials having amino functionality via formation of Schiff base linkage, resulting in the formation of OHA based hydrogel scaffolds. These hydrogels have recently attracted considerable attention as scaffolds for tissue engineering (TE) applications. Thus, the aim of the present review is to give an overview on OHA based hydrogels as scaffolds and their potential application in the field of TE along with the method of synthesis and important properties of OHA.
Topics: Animals; Humans; Hyaluronic Acid; Hydrogels; Oxidation-Reduction; Periodic Acid; Tissue Engineering; Tissue Scaffolds
PubMed: 31284008
DOI: 10.1016/j.ijbiomac.2019.07.014 -
Archives of Ophthalmology (Chicago,... Jul 1962
Topics: Coloring Agents; Humans; Periodic Acid; Retina; Staining and Labeling
PubMed: 13890263
DOI: 10.1001/archopht.1962.00960030066013 -
Experientia Jun 1961
Topics: Oxidation-Reduction; Periodic Acid; Steroids
PubMed: 13711595
DOI: 10.1007/BF02161423 -
Nature Jun 1953
Topics: Amino Acids; Amino Acids, Acidic; Carbohydrates; Periodic Acid; Sweetening Agents
PubMed: 13072519
DOI: 10.1038/1711123b0 -
Analytical Biochemistry May 2016A novel approach to the quantification of extracellular polysaccharides in miniaturized biofilms presenting a wide variety of extracellular matrices was developed. The...
A novel approach to the quantification of extracellular polysaccharides in miniaturized biofilms presenting a wide variety of extracellular matrices was developed. The assay used the periodic acid-Schiff reagent and was first calibrated on dextran and alginate solutions. Then it was implemented on 24-h and 48-h biofilms from three strains known to produce different exopolymeric substances (Pseudomonas aeruginosa, Bacillus licheniformis, Weissella confusa). The assay allowed quantification of the total exopolysaccharides, taking into account possible interferences due to cells or other main expolymers of the matrix (eDNA, proteins).
Topics: Biofilms; Periodic Acid; Polysaccharides; Rosaniline Dyes
PubMed: 26800864
DOI: 10.1016/j.ab.2016.01.008