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Nephrology, Dialysis, Transplantation :... Oct 1998
Review
Topics: Animals; Ascitic Fluid; Eosinophilia; Eosinophils; Humans; Kidney Diseases; Mice; Peritoneal Dialysis
PubMed: 9794540
DOI: 10.1093/ndt/13.10.2463 -
The Veterinary Clinics of North... Jan 2017Canine peritoneal fluid analysis results were retrospectively reviewed to assess the appropriateness of different classification schemes. Cutoffs of 3000 cells/μL and... (Review)
Review
Canine peritoneal fluid analysis results were retrospectively reviewed to assess the appropriateness of different classification schemes. Cutoffs of 3000 cells/μL and 2.5 g/dL protein are recommended. Analyzing the total nucleated cell count and total protein concentration is only the first step in peritoneal fluid analysis; microscopic examination, clinical presentation, and other laboratory data are all important in determining the final classification of peritoneal fluid analysis, keeping in mind that the most important aspect of fluid analysis is not what something is called, but whether it helps achieve a diagnosis. Discussion of effusion mechanisms, study observations, and recommended diagnostic steps after fluid analysis are included.
Topics: Animals; Ascitic Fluid; Cytological Techniques; Dog Diseases; Dogs
PubMed: 27531039
DOI: 10.1016/j.cvsm.2016.07.008 -
Veterinary Medicine, Small Animal... Aug 1980
Topics: Animals; Ascitic Fluid; Female; Horses; Male
PubMed: 6903041
DOI: No ID Found -
Minerva Ginecologica Aug 2003Endometriosis is a puzzling disorder with obscure pathogenesis. The objective of this review was to evaluate the complex role of peritoneal fluid in the etiopathogenesis... (Review)
Review
Endometriosis is a puzzling disorder with obscure pathogenesis. The objective of this review was to evaluate the complex role of peritoneal fluid in the etiopathogenesis of endometriosis. Several studies suggest that peritoneal fluid is a key inflammatory environment associated with endometriosis. Many active substances (cytokines, growth factors, hormones and oxidative stress parameters) have been identified in endometriosis patients at different stages of the disease. Inflammatory mediators may be involved in the endometriosis associated-infertility and possibly pain. Furthermore, these mediators may represent a non surgical method for diagnosing endometriosis. Better understanding of the mechanism of cytokines, growth factor and reactive oxygen species production and detoxification and further investigation of their effects on the peritoneal fluid environment are essential to obtain new insight into this disease and eventually develop novel diagnostic and therapeutic remedies.
Topics: Ascitic Fluid; Endometriosis; Female; Humans; Immunologic Factors; Infertility, Female; Oxidative Stress
PubMed: 14581858
DOI: No ID Found -
Acta Obstetricia Et Gynecologica... 2007This essay discusses the potential contribution of peritoneal fluid to the regulation of reproductive processes in female mammals. After noting the relatively high... (Review)
Review
This essay discusses the potential contribution of peritoneal fluid to the regulation of reproductive processes in female mammals. After noting the relatively high concentrations of diverse hormones in peritoneal fluid, and accepting that it bathes the surface of all the internal reproductive organs, peritoneal fluid is then proposed as a means of communication between the two ovaries. It could act to influence both the hierarchy of Graafian follicles and the rate and extent of development of a newly-formed corpus luteum. Cytokines in peritoneal fluid are considered in this context, as are the differing populations of leukocytes. Circumstantial evidence is offered for entry of peritoneal fluid into the Fallopian tubes, especially close to the time of ovulation, with the suggestion that such entry could modify the nature of endosalpingeal secretion and transudation. Thereafter, the spectrum of gonadal hormones in peritoneal fluid could be influencing uterine tissues in a local manner. Finally, reference is made to regional differences in the concentration of steroid hormones in the peritoneal fluid of women, likewise to regional differences in domestic animals.
Topics: Animals; Ascitic Fluid; Female; Genitalia, Female; Gonadal Steroid Hormones; Humans; Menstrual Cycle; Ovulation
PubMed: 17364298
DOI: 10.1080/00016340601155098 -
Nephron Feb 1999
Review
Topics: Ascitic Fluid; Eosinophilia; Humans; Kidney Failure, Chronic; Peritoneal Dialysis
PubMed: 9933745
DOI: 10.1159/000045266 -
European Journal of Obstetrics,... Jun 2008Endometriosis is a frequent pathology for which the dominant signs and symptoms are pelvic pain and infertility. The physiopathology remains the subject of controversy.... (Review)
Review
Endometriosis is a frequent pathology for which the dominant signs and symptoms are pelvic pain and infertility. The physiopathology remains the subject of controversy. Four physiopathological hypotheses have been put forward: regurgitation, metaplasia, induction and (vascular and lymphatic) embolization. The anatomical distribution of endometriotic lesions would appear to be fundamental for a better understanding of Sampson's menstrual regurgitation theory of endometriosis. Analysis of the results in the literature and comparison with our experience clearly shows that the distribution of endometriotic lesions is asymmetrical in several respects. Abdominopelvic anatomy and peritoneal fluid flow can explain this asymmetrical distribution of endometriotic lesions in the great majority of cases. These observations are a very strong argument in favour of the crucial role played by tubal regurgitation and the peritoneal fluid in the physiopathology of endometriosis. The similarity in anatomical distribution of endometriomas, superficial and deeply invasive endometriotic lesions would tend to indicate a common origin for these different types of lesions.
Topics: Ascitic Fluid; Endometriosis; Female; Humans; Menstruation
PubMed: 18336988
DOI: 10.1016/j.ejogrb.2008.01.014 -
Diagnostic Cytopathology Jan 2023
Topics: Humans; Ascitic Fluid
PubMed: 36239423
DOI: 10.1002/dc.25063 -
Fertility and Sterility Feb 2020To demonstrate the feasibility of studying exosomes directly from peritoneal fluid, we isolated exosomes from endometriosis patient samples and from controls, and...
OBJECTIVE
To demonstrate the feasibility of studying exosomes directly from peritoneal fluid, we isolated exosomes from endometriosis patient samples and from controls, and characterized their cargo.
DESIGN
Case-control experimental study.
SETTING
Academic clinical center.
PATIENT (S)
Women with and without endometriosis who underwent laparoscopic surgery (n = 28 in total).
INTERVENTION (S)
None.
MAIN OUTCOME MEASURE (S)
Concentration of exosomes within peritoneal fluid and protein content of the isolated exosomes.
RESULT (S)
Peritoneal fluid samples were pooled according to the cycle phase and disease stage to form six experimental groups, from which the exosomes were isolated. Exosomes were successfully isolated from peritoneal fluid in all the study groups. The concentration varied with cycle phase and disease stage. Proteomic analysis showed specific proteins in the exosomes derived from endometriosis patients that were absent in the controls. Five proteins were found exclusively in the endometriosis groups: PRDX1, H2A type 2-C, ANXA2, ITIH4, and the tubulin α-chain.
CONCLUSION (S)
Exosomes are present in peritoneal fluid. The characterization of endometriosis-specific exosomes opens up new avenues for the diagnosis and investigation of endometriosis.
Topics: Adult; Annexin A2; Ascitic Fluid; Case-Control Studies; Endometriosis; Exosomes; Feasibility Studies; Female; Histones; Humans; Middle Aged; Peroxiredoxins; Proteinase Inhibitory Proteins, Secretory; Proteins; Proteomics; Tubulin; Young Adult
PubMed: 32106990
DOI: 10.1016/j.fertnstert.2019.09.032 -
Expert Review of Molecular Diagnostics Apr 2015Endometriosis is defined as the presence of endometrial glands and stroma outside the uterus, in different parts of the peritoneal cavity. It affects up to 10% of... (Review)
Review
Endometriosis is defined as the presence of endometrial glands and stroma outside the uterus, in different parts of the peritoneal cavity. It affects up to 10% of reproductive-age women and up to 50% of women with infertility. Surgical diagnosis of endometriosis is still the gold standard, with no diagnostic biomarkers available. Medical therapies target the symptoms and not the disease itself, and surgical treatment cannot prevent recurrence. As peritoneal fluid reflects the altered peritoneal microenvironment of endometriosis patients, it can provide a wealth of information on pathophysiological processes and potential biomarkers. This report reviews the molecular biomarkers identified in peritoneal fluid over the last 5 years and discusses their potential applications for diagnosis, prognosis and drug therapy.
Topics: Adult; Ascitic Fluid; Biomarkers; Endometriosis; Female; Humans; Inflammation; Oxidative Stress
PubMed: 25719220
DOI: 10.1586/14737159.2015.1015994