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Annals of Agricultural and... Dec 2018Data on the possible role of peritoneal fluid free radical-mediated oxidative damage in the pathogenesis of endometriosis still remains inconsistent. The aim of the...
INTRODUCTION
Data on the possible role of peritoneal fluid free radical-mediated oxidative damage in the pathogenesis of endometriosis still remains inconsistent. The aim of the study was to determine iron metabolism markers and their influence on oxidative stress arameters in the peritoneal fluid of women with endometriosis.
MATERIAL AND METHODS
110 women with endometriosis and 119 patients with benign ovarian cysts were included in the study. All visible peritoneal fluid was aspirated during laparoscopy from the anterior and posterior cul-de-sacs. under direct vision to avoid blood contamination. Haemoglobin, iron, total oxidative status, and total antioxidant status were measured using standard colourimetric kits.
RESULTS
Haemoglobin, iron levels, as well as total oxidative status values were significantly higher, whereas total antioxidant status values were significantly lower in the peritoneal fluid of patients with endometriosis, in comparison to the reference groups. No differences were observed in peritoneal fluid concentrations of all parameters measured in relation to the phase of the menstrual cycle.
CONCLUSIONS
Peritoneal fluid of women with endometriosis is characterized by disrupted iron metabolism. This is most likely related to an increased number of erythrocytes in the peritoneal cavity of endometriotic women, which leads to a higher concentration of haemoglobin in this environment. Impaired iron homeostasis may have a significant influence on the pathophysiology of peritoneal endometriosis by the direct impact of haemoglobin derivatives and/or formation of the pro-inflammatory and pro-oxidative environment. Peritoneal cavity oxidative stress occurs predominantly in women in advanced stages of the disease.
Topics: Adolescent; Adult; Ascitic Fluid; Biomarkers; Endometriosis; Female; Hemoglobins; Humans; Iron; Laparoscopy; Middle Aged; Ovarian Cysts; Oxidative Stress; Peritoneal Cavity; Young Adult
PubMed: 30586986
DOI: 10.26444/aaem/75802 -
Cytopathology : Official Journal of the... May 2024
Topics: Humans; Ascitic Fluid; Cyamopsis; Ascites
PubMed: 38126698
DOI: 10.1111/cyt.13351 -
Modern Pathology : An Official Journal... Jul 2003To determine whether genetic abnormalities present in primary ovarian tumors can be used to detect cancer cells in peritoneal fluid, we tested 14 ovarian cancers and 1...
To determine whether genetic abnormalities present in primary ovarian tumors can be used to detect cancer cells in peritoneal fluid, we tested 14 ovarian cancers and 1 benign tumor of the ovary for loss of heterozygosity (LOH) at chromosomal arms 13q, 17p, 17q, and 22q and for mutations in the p53 and K-ras genes. In each case, matched primary tumor, normal tissue, and peritoneal fluid were analyzed. The highest frequency of LOH was found on chromosomal arm 17p (42%), followed by chromosomal arm 17q (36%), 22q (30%), and 13q (21%). Identical alterations were detected in matched peritoneal fluid (either peritoneal wash or ascitic fluid) in 3 of the 8 patients with LOH in the tumor (38%). Direct sequence analysis detected p53 mutations in 3 of the 14 malignant tumors (21%) and no (0) K-ras mutations. Identical mutations were detected in matched peritoneal fluid from all 3 patients with p53 mutations. All 8 of the 14 (57%) malignant tumors that showed at least one genetic abnormality were serous adenocarcinoma and identical alterations were detected in 5 of the 8 (62%) matched peritoneal fluid samples. Our findings indicate that molecular abnormalities can be detected in peritoneal fluid from patients with ovarian cancer and may be used to complement current conventional diagnostic procedures for detection of primary ovarian cancer.
Topics: Adenocarcinoma; Ascitic Fluid; Base Pair Mismatch; Chromosomes, Human, Pair 13; Chromosomes, Human, Pair 17; Chromosomes, Human, Pair 22; DNA Mutational Analysis; DNA, Neoplasm; Female; Genes, p53; Genes, ras; Genetic Markers; Humans; Loss of Heterozygosity; Microdissection; Microsatellite Repeats; Ovarian Neoplasms; Polymerase Chain Reaction
PubMed: 12861058
DOI: 10.1097/01.MP.0000076979.28106.ED -
Fertility and Sterility Apr 2011Cell proliferation of endometrial stromal cells treated with peritoneal fluid of women (aged 25 to 43 years) with endometriosis (n = 12) statistically significantly...
Cell proliferation of endometrial stromal cells treated with peritoneal fluid of women (aged 25 to 43 years) with endometriosis (n = 12) statistically significantly increased compared with the control treatment (peritoneal fluid of women without endometriosis, n = 8). Also, COX-2 gene expression and prostaglandin E(2) production were induced in those cells by increasing COX-2 promoter transcription activity, which could be attenuated by a specific p38MAPK inhibitor, suggesting a role for peritoneal fluid in the etiopathogenesis of endometriosis.
Topics: Adult; Ascitic Fluid; Cell Proliferation; Cells, Cultured; Culture Media, Conditioned; Cyclooxygenase 2; Endometriosis; Endometrium; Female; Gene Expression Regulation, Enzymologic; Humans; Peritoneal Diseases; Prostaglandins; Stromal Cells; Up-Regulation
PubMed: 21145050
DOI: 10.1016/j.fertnstert.2010.11.039 -
Journal of the American Veterinary... Jun 1985Peritoneal fluid was collected from 15 clinically normal horses and was analyzed for nucleated cell (NC) counts and specific gravity. Six horses (controls, group 1) were...
Peritoneal fluid was collected from 15 clinically normal horses and was analyzed for nucleated cell (NC) counts and specific gravity. Six horses (controls, group 1) were subjected to abdominocentesis only, with a teat cannula, every 24 hours for 5 days. There were no marked changes in the peritoneal fluid of these horses over the 5-day period. Peritoneal fluid was collected from 6 other horses (group 2) with an 8.89-cm 18-gauge needle. The needle was then advanced until intestinal fluid was obtained. Peritoneal fluid was then collected with teat cannulas at 24-hour intervals for an additional 4 days. Peritoneal fluid NC counts from group 2 horses were significantly increased (P less than 0.05) at peak values 2 days after enterocentesis. Specific gravities of peritoneal fluid from group 2 horses were increased on days 1 and 2 after enterocentesis (P greater than 0.05). Peritoneal fluid from 3 other horses (group 3) was collected before enterocentesis (base line) and again at 4-hour intervals after enterocentesis. Peritoneal fluid NC counts of group 3 horses were markedly increased above base-line values 4 hours after enterocentesis and continued to increase for up to 12 hours after enterocentesis when the experiment was terminated. All horses that underwent enterocentesis remained clinically normal except 1 group 3 horse that had a fever (39.6 C) 24 hours after enterocentesis.
Topics: Animals; Ascitic Fluid; Horse Diseases; Horses; Intestines; Leukocyte Count; Punctures; Specific Gravity
PubMed: 4019289
DOI: No ID Found -
Veterinary Surgery : VS 1988Ten student surgery ponies were subjected to exploratory laparotomy. Abdominal paracentesis was performed preoperatively and daily postoperatively for 6 days, then the...
Ten student surgery ponies were subjected to exploratory laparotomy. Abdominal paracentesis was performed preoperatively and daily postoperatively for 6 days, then the ponies were euthanatized and necropsied. Initial baseline peritoneal fluid parameters were within established reference limits. Postoperatively, the total leukocyte count and total protein in the peritoneal fluid rose and remained elevated for the 6 days of the study. Complete blood counts (CBCs) were performed preoperatively and on days 1 and 4 postoperatively. On day 1, a stress leukogram with a mild inflammatory component developed, but by day 4, the CBCs were within normal limits. The mean plasma fibrinogen levels, which were determined daily, peaked on day 4.
Topics: Animals; Ascitic Fluid; Horses; Laparotomy; Leukocyte Count; Reference Values
PubMed: 3256144
DOI: 10.1111/j.1532-950x.1988.tb00268.x -
Equine Veterinary Journal Sep 1990Peritoneal fluid was analysed from 17 foals, aged 13 to 134 days with a mean age of 68 days. Cytologically, the peritoneal fluid was characterised by a mean total cell...
Peritoneal fluid was analysed from 17 foals, aged 13 to 134 days with a mean age of 68 days. Cytologically, the peritoneal fluid was characterised by a mean total cell count of 0.45 x 10(9)/litre (range 0.06 to 1.42 x 10(9)/litre), rare eosinophils, rare cytophagia and variable percentages of neutrophils and mononuclear cells. These data indicate that peritoneal fluid nucleated cell counts over 1.50 x 10(9)/litre in the foal should be interpreted as elevated. Biochemical evaluation revealed a mean biuret protein level of 12 g/litre, mean refractive index protein level of 16 g/litre and urea nitrogen concentration of 1.96 mmol/litre. There was no correlation between the foals' white blood cell and peritoneal fluid nucleated cell counts. Results of this study indicate that adult horse reference values for evaluation of peritoneal fluid are of questionable validity for foals. Diagnostically, the most important observation was that maximum peritoneal fluid nucleated cell counts in healthy foals were much lower than reported maximal reference values for adult horses (1.5 x 10(9)/litre versus 5.0 x 10(9)/litre or 10.0 x 10(9)/litre).
Topics: Animals; Animals, Newborn; Ascitic Fluid; Blood Proteins; Blood Urea Nitrogen; Horses; Leukocyte Count; Peritoneal Cavity; Reference Values; Urea
PubMed: 2226402
DOI: 10.1111/j.2042-3306.1990.tb04290.x -
Veterinary Clinical Pathology Jun 2006To the authors' knowledge, there is no information in the literature about normal peritoneal fluid values in ovine species.
BACKGROUND
To the authors' knowledge, there is no information in the literature about normal peritoneal fluid values in ovine species.
OBJECTIVES
The purpose of the study reported here was to establish reference intervals for peritoneal fluid from clinically normal Awassi sheep and to compare the values to those in blood.
METHODS
Peritoneal fluid and blood samples were collected into tubes containing EDTA, from 40 clinically healthy, nonpregnant, female Awassi sheep, aged 2 to 7 years. Total nucleated cell count (TNCC) was determined using an electronic cell counter. Total protein, albumin, urea, creatinine, and glucose concentrations and aspartate transaminase activity were analyzed using commercially available kits.
RESULTS
TNCC (mean +/- SD) of peritoneal fluid was 1.1 +/- 0.87 X 10(3)/microl, with neutrophils (3.9%), lymphocytes (33.5%), macrophages/monocytes (61.2%), and eosinophils (1.4%). Biochemical results in peritoneal fluid were: total protein, 1.7 +/- 0.74 g/dL; albumin, 1.0 +/- 0.04 g/dL; urea, 12.6 +/- 3.95 mg/dL; creatinine, 0.6 +/- 0.19 mg/dL; glucose, 54.8 +/- 6.11 mg/dL; and aspartate transaminase, 23.5 +/- 8.82 U/L. Eosinophil percentage and creatinine concentration did not differ significantly from blood values.
CONCLUSION
Baseline values for cytologic and biochemical parameters in peritoneal fluid of Awassi sheep, with comparison to blood, have been generated. Such data may be applicable to other ovine species and can be used in the clinical investigation of ovine abdominal disorders.
Topics: Animals; Ascitic Fluid; Female; Reference Values; Sheep
PubMed: 16783716
DOI: 10.1111/j.1939-165x.2006.tb00117.x -
Journal of the American Veterinary... Oct 1996To characterize, in mares, changes in peritoneal fluid that occurred within the first 7 days after routine foaling.
OBJECTIVE
To characterize, in mares, changes in peritoneal fluid that occurred within the first 7 days after routine foaling.
DESIGN
Prospective observational trial.
ANIMALS
15 mares.
PROCEDURE
Abdominocentesis was performed within 10 days before foaling and again 12 hours, 3 days, and 7 days after each horse foaled. Data recorded for each sample included total nucleated cell count, differential cell count, specific gravity, fibrinogen concentration, and total protein concentration. Smears of each sample were examined by a single clinical pathologist.
RESULTS
There were not any significant differences over time in specific gravity, total protein concentration, fibrinogen concentration, total nucleated cell count, or number of small mononuclear cells. Mean numbers of neutrophils and large mononuclear cells in samples collected after foaling were significantly higher than mean numbers in samples collected before foaling. For 11 of 14 horses, all samples were characterized cytologically as transudates without cytologic abnormalities.
CLINICAL IMPLICATIONS
Results of analysis of peritoneal fluid from peripartum mares suggest that nucleated cell count, protein concentration, and specific gravity of peritoneal fluid from mares that have recently foaled should be normal. Thus, peritoneal fluid abnormalities detected in mares within a week after foaling should usually be attributed to a systemic or gastrointestinal problem and not to the foaling process itself.
Topics: Animals; Ascitic Fluid; Cell Count; Exudates and Transudates; Female; Horses; Postpartum Period; Prospective Studies; Proteins; Reference Values; Specific Gravity
PubMed: 8837651
DOI: No ID Found -
Veterinary Surgery : VS Jun 2011To describe peritoneal drain fluid volume, fluid cytology, and blood-to-peritoneal fluid lactate and glucose concentration differences after exploratory celiotomy in...
OBJECTIVE
To describe peritoneal drain fluid volume, fluid cytology, and blood-to-peritoneal fluid lactate and glucose concentration differences after exploratory celiotomy in normal dogs.
STUDY DESIGN
Prospective study.
ANIMALS
Healthy Beagle dogs (n=10).
METHODS
After exploratory celiotomy, a peritoneal drain was placed, and peritoneal fluid was recorded every 6 hours for 7 days. Fluid was submitted for cytologic examination, and fluid and blood glucose and lactate concentrations were recorded every 12 hours. On day 7, drains were removed and drain tips submitted for aerobic bacterial culture.
RESULTS
Mean peritoneal fluid volume decreased from 2.8 mL/kg/day (day 1) to 0.6 mL/kg/day (day 7). All dogs had degenerate neutrophils in peritoneal fluid throughout the 7 days. Four dogs developed contaminated drains. Blood-to-peritoneal glucose concentration differences > 20 mg/dL occurred after day 4. By day 7, 5 of 7 dogs with patent drains had blood-to-peritoneal lactate concentration differences < -2 mmol/L.
CONCLUSION
After day 4, blood-to-peritoneal glucose concentration differences were consistent with septic effusion based on previously reported values used to diagnose septic peritonitis in dogs. Blood-to-peritoneal lactate concentration differences varied but after day 4, >70% of dogs had differences consistent with septic peritonitis each day. Postoperative blood-to-peritoneal fluid glucose and lactate difference may not be reliable indicators of septic peritonitis when evaluating abdominal fluid collected with closed suction drains.
Topics: Animals; Ascitic Fluid; Blood Glucose; Dogs; Female; Glucose; Lactic Acid; Male
PubMed: 21466565
DOI: 10.1111/j.1532-950X.2011.00799.x