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Disaster Medicine and Public Health... Jun 2020
Topics: COVID-19; Coronavirus Infections; Humans; Pandemics; Pneumonia, Viral; Quarantine; Ships
PubMed: 32241332
DOI: 10.1017/dmp.2020.67 -
JAMA Apr 2023
PubMed: 37000446
DOI: 10.1001/jama.2023.3664 -
Endeavour 2019Richard Julius Petri's status as inventor of the culture dish that bears his name has been subject to a number of challenges over the years. Both those bacteriologists...
Richard Julius Petri's status as inventor of the culture dish that bears his name has been subject to a number of challenges over the years. Both those bacteriologists who claimed self-recognition for the invention, and those to whom it was attributed by their various advocates were all contemporaries of Petri. The evidence assembled here indicates that no single individual-including Petri-ought to be accorded credit for the inception of that shallow, circular, covered culture dish which, it transpires, is a simultaneous invention made by half a dozen bacteriologists active in the mid-1880s and ultimately owes its emergence to the prevailing bacteriological zeitgeist.
Topics: Bacteriology; Culture Media; History, 19th Century; Humans; Inventions
PubMed: 31030894
DOI: 10.1016/j.endeavour.2019.04.001 -
Journal of Health Care For the Poor and... 2020This commentary amplifies the insidious nature of the novel coronavirus (resulting in COVID19) and its ubiquitous spread, which disproportionately and adversely affect...
This commentary amplifies the insidious nature of the novel coronavirus (resulting in COVID19) and its ubiquitous spread, which disproportionately and adversely affect the health and well-being of people of color. The consequence is poor health outcomes and premature death. Ample previous literature documents health inequities in the morbidity and mortality statistics for Black and Brown people in the United States. Their excess deaths are due to disproportionately high rates of serious health conditions (diabetes; hypertension; asthma; and lung, kidney, and heart disease), as well as structural factors having to do with income, employment, and the built environment in which they live. The health conditions are exacerbated with ongoing societal problems and stress emerging from the country's history of dehumanizing racial inequities. Current discrimination comes most virulently in the form of systematic and institutionalized racist policies that keep racial and ethnic minorities marginalized and disempowered. Furthermore, people of color encounter the immediate external pressures of working away from home and using public transportation during the country's extraordinary ongoing lockdown, heightening the risk of exposure to the virus. Moreover, the same population is overrepresented in jails and prisons where social distancing is impossible. Any virulent virus without a vaccine is bound to become a human petri dish in which people of color in the U.S. today are caught. The war against the coronavirus for people of color is part and parcel of the war to eliminate historic inequities and to level the socioeconomic playing field. This article covers the racial/ethnic inequities in morbidity and mortality from COVID19 and the slow and untimely response by the federal government to address mediation of the spread of the virus. For people of color to transcend the coronavirus pandemic crisis there must be comprehensive access to COVID-19 testing and early, sustained, and affordable access to health care, including hospitalization. Such access will require national leadership, which seems to be in short supply.
Topics: Black or African American; COVID-19; Health Status Disparities; Humans; United States
PubMed: 33410785
DOI: 10.1353/hpu.2020.0037 -
Journal of Ophthalmology 2020To develop a method for the rapid isolation of rat RPE cells with high yield and maintain its epithelial state in modified culture system.
OBJECTIVE
To develop a method for the rapid isolation of rat RPE cells with high yield and maintain its epithelial state in modified culture system.
METHODS
The eyeballs were incubated with dispase. The retina was isolated with RPE attached and cut into several pieces. Following a brief incubation in growth medium, large RPE sheets can be harvested rapidly. RPE cells were divided into four groups and cultured for several weeks, that is, (1) in cell culture dishes with 10% FBS containing medium (CC dish-FBS), (2) in petri dishes with 10% FBS containing medium (Petri dish-FBS), (3) in cell culture dishes with N2 and B27 containing medium (CC dish-N2B27), and (4) in petri dishes with N2 and B27 containing medium (Petri dish-N2B27). Morphological and biological characteristics were investigated using light microscopy, Q-PCR, and western blot.
RESULTS
The retina would curl inwardly during the growth medium incubation period, releasing RPE sheets in the medium. Compared with low density group (5,000 cells/cm), RPE cells plated at high density (15,000 cells/cm) can maintain RPE morphology for a more extended period. Meanwhile, plating RPE cells at low density significantly reduced the expression of RPE cell type-specific genes (RPE65, CRALBP, and bestrophin) and increased the expression of EMT-related genes (N-cadherin, fibronectin, and -SMA), in comparison with the samples from the high density group. The petri dish culture condition reduced cell adhesion and thus inhibited RPE cell proliferation. As compared with other culture conditions, RPE cells in the petri dish-N2B27 condition could maintain RPE phenotype with increased expression of RPE-specific genes and decreased expression of EMT-related genes. The AKT/mTOR pathway was also decreased in petri dish-N2B27 condition.
CONCLUSION
The current study provided an alternative method for easy isolation of RPE cells with high yield and maintenance of its epithelial morphology in the petri dish-N2B27 condition.
PubMed: 32855817
DOI: 10.1155/2020/4892978 -
Advances in Applied Microbiology 2021Petri dish contaminations are commonplace and personally witnessed by every microbiologist. The vast majority of such contaminations result in nothing more than...
Petri dish contaminations are commonplace and personally witnessed by every microbiologist. The vast majority of such contaminations result in nothing more than annoyance following which the Petri dishes are discarded. However, a handful of incidents of contaminations have led to momentous outcomes, the most renowned of which being that perceived by Alexander Fleming on the basis of the immense number of lives saved by penicillin. Petri dish contaminations as reported upon in the literature fall broadly into two categories; those in which the contaminant caused antagonism toward the species being cultured, and those in which the contaminant was established to be a species novum. Accounts of both of these categories of contaminations are set out here.
Topics: Culture Media
PubMed: 34353504
DOI: 10.1016/bs.aambs.2021.04.002 -
Langmuir : the ACS Journal of Surfaces... Nov 2023Hydrophilicity is a requisite attribute for the 2D cell culture substrate's surface, facilitating cell adhesion and spreading. Conventional poly(dimethylsiloxane) (PDMS)...
Hydrophilicity is a requisite attribute for the 2D cell culture substrate's surface, facilitating cell adhesion and spreading. Conventional poly(dimethylsiloxane) (PDMS) microfluidic chips necessitate protein coatings to enhance hydrophilicity; however, this approach is afflicted by issues of transient efficacy, interference with cell analysis, and high costs. This paper presents a protein-free microfluidic chip, termed a "microfluidic Petri dish-chip (MPD-chip)", integrating PDMS as the cover and a tissue culture-treated (TC-treated) Petri dish as the substrate. Microstructures are hot-embossed onto the Petri dish substrate using a silicon mold. This meticulous replication process serves to establish stable flow field dynamics within the chip. A simplified method for irreversible bonding, utilizing plasma activation and silylation, is proposed for affixing the PDMS cover onto the microstructured Petri dish substrate. The prepared composite chip exhibits remarkable tightness, boasting a notable bond strength of 2825 kPa. Furthermore, the composite microfluidic chip demonstrates the capability to withstand flow velocities of at least 200 μL/min, effectively meeting the required injection standards for both cell suspension and culture medium. SH-SY5Y and HeLa cells are cultured dynamically in the MPD-chip and control groups. Outcomes encompassing normalized cell density, cell adhesion area, and cell viability metrics unequivocally highlight the superiority of the MPD-chip in facilitating long-term two-dimensional (2D) cell cultures.
Topics: Humans; Microfluidics; Microfluidic Analytical Techniques; HeLa Cells; Neuroblastoma; Cell Culture Techniques; Proteins
PubMed: 37906157
DOI: 10.1021/acs.langmuir.3c01982 -
Current Protocols in Molecular Biology Jan 2019We provide protocols for titering and isolating bacterial colonies from single cells by serial dilutions, for streaking agar plates, and for spreading suspensions of...
We provide protocols for titering and isolating bacterial colonies from single cells by serial dilutions, for streaking agar plates, and for spreading suspensions of cells on plates. Support protocols describe replica plating and methods for storing strains as agar stabs and frozen stocks. © 2018 by John Wiley & Sons, Inc.
Topics: Agar; Bacteriological Techniques; Colony Count, Microbial; Culture Media; Escherichia coli; Preservation, Biological
PubMed: 30414382
DOI: 10.1002/cpmb.82 -
PHAGE (New Rochelle, N.Y.) Dec 2021Bacteriophage plaque enumeration is a critical step in a wide array of protocols. The current gold standard for plaque enumeration on Petri dishes is through manual...
Bacteriophage plaque enumeration is a critical step in a wide array of protocols. The current gold standard for plaque enumeration on Petri dishes is through manual counting. However, this approach is not only time-consuming and prone to human error but also limited to Petri dishes with countable number of plaques resulting in low throughput. We present OnePetri, a collection of trained machine learning models and open-source mobile application for the rapid enumeration of bacteriophage plaques on circular Petri dishes. When compared against the current gold standard of manual counting, OnePetri was ∼30 × faster. Compared against other similar tools, OnePetri had lower relative error (∼13%) than Plaque Size Tool (PST) (∼86%) and CFU.AI (∼19%), while also having significantly reduced detection times over PST (1.7 × faster). The OnePetri application is a user-friendly platform that can rapidly enumerate phage plaques on circular Petri dishes with high precision and recall.
PubMed: 36159886
DOI: 10.1089/phage.2021.0012 -
International Journal of Infectious... Sep 2007
Topics: Agar; Animals; Diarrhea; Female; Humans; Larva Migrans; Strongyloides stercoralis; Strongyloidiasis
PubMed: 17331785
DOI: 10.1016/j.ijid.2006.12.007