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Scientific Reports 2013Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is a widespread method. We demonstrate that both fluorescent...
Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is a widespread method. We demonstrate that both fluorescent and unlabeled live cells in a Petri dish observed with a microscope can be automatically recognized by computer vision and picked up by a computer-controlled micropipette. This method can be routinely applied as a FACS down to the single cell level with a very high selectivity. Sorting resolution, i.e., the minimum distance between two cells from which one could be selectively removed was 50-70 micrometers. Survival rate with a low number of 3T3 mouse fibroblasts and NE-4C neuroectodermal mouse stem cells was 66 ± 12% and 88 ± 16%, respectively. Purity of sorted cultures and rate of survival using NE-4C/NE-GFP-4C co-cultures were 95 ± 2% and 62 ± 7%, respectively. Hydrodynamic simulations confirmed the experimental sorting efficiency and a cell damage risk similar to that of normal FACS.
Topics: 3T3 Cells; Animals; Animals, Newborn; Astrocytes; Cell Line; Cell Separation; Cell Survival; Cells, Cultured; Coculture Techniques; Flow Cytometry; Green Fluorescent Proteins; HEK293 Cells; Humans; Image Processing, Computer-Assisted; Keratinocytes; Mice; Mice, Transgenic; Microglia; Microscopy, Fluorescence; Microscopy, Phase-Contrast; Microscopy, Video; Reproducibility of Results
PubMed: 23336070
DOI: 10.1038/srep01088 -
Data in Brief Apr 2023Recent advancements in image analysis and interpretation technologies using computer vision techniques have shown potential for novel applications in clinical...
Recent advancements in image analysis and interpretation technologies using computer vision techniques have shown potential for novel applications in clinical microbiology laboratories to support task automation aiming for faster and more reliable diagnostics. Deep learning models can be a valuable tool in the screening process, helping technicians spend less time classifying no-growth results and quickly separating the categories of tests that deserve further analysis. In this context, creating datasets with correctly classified images is fundamental for developing and improving such models. Therefore, a dataset of urine test Petri dishes images was collected following a standardized process, with controlled conditions of positioning and lighting. Image acquisition was conducted by applying a hardware chamber equipped with a led lightning source and a smartphone camera with 12 MP resolution. A software application was developed to support image classification and handling. Experienced microbiologists classified the images according to the positive, negative, and uncertain test results. The resulting dataset contains a total of 1500 images and can support the development of deep learning algorithms to classify urine exams according to their microbial growth.
PubMed: 36942098
DOI: 10.1016/j.dib.2023.109034 -
Zeitschrift Fur Psychosomatische... Dec 2020
Topics: Behavioral Research; Humans; Mental Health
PubMed: 33284074
DOI: 10.13109/zptm.2020.66.4.418 -
Applied Microbiology Sep 1970Teflon spaghetti tubing was placed over the metal needles of the dispensing head of the Accu-Drop multidropper, thus preventing the delivery of multiple drops. A metal...
Teflon spaghetti tubing was placed over the metal needles of the dispensing head of the Accu-Drop multidropper, thus preventing the delivery of multiple drops. A metal plate was drilled so that 25 cartridges could be positioned to inoculate simultaneously 25 compartments in a divided petri dish.
Topics: Bacteriocins; Bacteriological Techniques; Bacteriophages; Methods; Microbiology
PubMed: 4922155
DOI: 10.1128/am.20.3.517-518.1970 -
Physics in Medicine and Biology Jul 2005We carried out a numerical dosimetry study for in vitro experiments on millimetre-wave (MMW) biological effects on cells. The cell layers are cultured in 35 mm Petri...
We carried out a numerical dosimetry study for in vitro experiments on millimetre-wave (MMW) biological effects on cells. The cell layers are cultured in 35 mm Petri dishes placed in the far-field region of a rectangular horn irradiator generating a 50.0 GHz continuous sinusoidal MMW. The finite-difference time-domain (FDTD) method and the second-order approximation of the absorbing boundary conditions (ABCs) are applied in calculating the specific absorption rate (SAR) in the cells. The 0.125 mm and 0.25 mm voxel models of the Petri dish are used. The permittivity and the conductivity of the cells and those of the culture medium are obtained with Debye's dispersion equation. We measured the power pattern of the irradiator using the plane wave expansion (PWE) method, and developed a program module to calculate the FDTD-compatible incident field from the irradiator, whose position and orientation are adjustable. The MMW multiple reflection between the Petri dish and the irradiator is evaluated before being neglected. For the single-dish, double-dish and quadruple-dish exposure set-ups, the SAR intensity and the SAR uniformity are analysed and compared. The meniscus effect on the SAR distribution over the cell layer is evaluated for the single-dish set-up. The influence of the Petri dish interaction on the SAR distribution is examined for the double-dish and quadruple-dish set-ups.
Topics: Animals; Cell Culture Techniques; Cells, Cultured; Computer Simulation; Electromagnetic Fields; Equipment Design; Humans; Models, Biological; Radiation Dosage
PubMed: 16177518
DOI: 10.1088/0031-9155/50/14/015 -
Analytical Biochemistry Dec 2007We have developed a system that allows focal drug application for cell culture microscopy. Single-cell drug delivery is achieved through the insertion of a...
We have developed a system that allows focal drug application for cell culture microscopy. Single-cell drug delivery is achieved through the insertion of a patch-clamp-like micropipette in a microenvironment-controlled chamber mounted on a standard 35-mm Petri dish. The system has precise control of temperature, CO(2) concentration, and humidity, while preventing contamination during experiments. The use of standard Petri dishes allows long-term experiments by alternating in situ microscopy with incubator growth. Modern biological long-term experiments such as the characterization of drug effects on cell movement, axonal guidance, mitosis, apoptosis, differentiation, or volume regulation can be performed. The chamber is compatible with any inverted microscope without significant modifications.
Topics: Animals; Cells, Cultured; Cricetinae; Drug Delivery Systems; Equipment Design; Microscopy, Video; Time Factors
PubMed: 17884006
DOI: 10.1016/j.ab.2007.08.005 -
Hospital Practice (Office Ed.) Apr 1989
Topics: Anti-Bacterial Agents; England; Fungi; History, 20th Century; Penicillins
PubMed: 2495290
DOI: No ID Found -
Current Genetics Feb 1994We have developed rapid and economic methods for the isolation of nucleic acids from filamentous fungi. The main advantages of these methods are: (1) the mycelium is...
We have developed rapid and economic methods for the isolation of nucleic acids from filamentous fungi. The main advantages of these methods are: (1) the mycelium is directly recovered from a Petri-dish culture, (2) the complete experiment takes place in microfuge tubes, (3) it is very fast and allows for the processing of 24 samples in the same day, and (4) up to 100 micrograms of total DNA or RNA are recovered, both of which are sufficiently pure for most purposes. Of particular interest is the recovery of large amounts of mitochondrial DNA as visualised by electrophoresis in ethidium bromide-stained gels.
Topics: Culture Media; DNA, Fungal; Fungi; Mycology; RNA, Fungal; Time Factors; Ultracentrifugation
PubMed: 8087879
DOI: 10.1007/BF00309536 -
The Journal of Medical Laboratory... Oct 1958
Topics: Bacteriology; Humans
PubMed: 13588309
DOI: No ID Found -
AIChE Journal. American Institute of... Jun 2016Liquid in a Petri dish spontaneously circulates in a radial pattern, even when the dish is at rest. These fluid flows have been observed and utilized for biological...
Liquid in a Petri dish spontaneously circulates in a radial pattern, even when the dish is at rest. These fluid flows have been observed and utilized for biological research, but their origins have not been well-studied. Here we used particle-tracking to measure velocities of radial fluid flows, which are shown to be linked to evaporation. Infrared thermal imaging was used to identify thermal gradients at the air-liquid interface and at the bottom of the dish. Two-color ratiometric fluorescence confocal imaging was used to measure thermal gradients in the vertical direction within the fluid. A finite-element model of the fluid, incorporating the measured temperature profiles, shows that buoyancy forces are sufficient to produce flows consistent with the measured particle velocity results. Such flows may arise in other dish or plate formats, and may impact biological research in positive or negative ways.
PubMed: 27158150
DOI: 10.1002/aic.15194