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Report on Carcinogens : Carcinogen... 2011
Topics: Animals; Carcinogens; Humans; Indicators and Reagents; Laxatives; Lymphoma; Phenolphthalein
PubMed: 21863080
DOI: No ID Found -
Report on Carcinogens : Carcinogen... 2002
Topics: Animals; Carcinogens; Environmental Exposure; Government Regulation; Humans; Phenolphthalein; United States
PubMed: 15332290
DOI: No ID Found -
Journal of Materials Chemistry. B May 2021Because of increasing levels of obesity and the development of social network platforms, the online sale of slimming beverages has become very common. However, such...
Because of increasing levels of obesity and the development of social network platforms, the online sale of slimming beverages has become very common. However, such beverages are often reported to contain hazardous drugs, such as phenolphthalein. In the present study, we established a rapid and sensitive immunochromatography gold-labeled monoclonal antibody (mAb) (IGM) method and a fluorescence-labeled mAb (IFM) method for determining phenolphthalein in ten types of slimming tea. The monoclonal antibodies and coating antigens were produced in our laboratory. Under optimal conditions, the cut-off limits were 250 ng mL-1 (IFM strip) and 500 ng mL-1 (IGM strip) in both 0.01 M phosphate-buffered saline (PBS) and samples of slimming tea. The mean recoveries were 96.2 to 104.7% for the IGM strip and 90.7 to 104.7% for the IFM strip. The data showed that the IFM strip was more sensitive than the IGM strip and that results could be generated within 10 min. Consequently, this novel technique represents a rapid and convenient method with which to detect phenolphthalein.
Topics: Antibodies, Monoclonal; Beverages; Chromatography, Affinity; Gold; Immunoassay; Limit of Detection; Metal Nanoparticles; Phenolphthalein; Surface-Active Agents
PubMed: 33908585
DOI: 10.1039/d1tb00510c -
Journal of Pharmaceutical and... Apr 2022An anti-phenolphthalein monoclonal antibody (mAb) was prepared based on the N,N'-Carbonyldiimidazole (CDI) method through phenolphthalein conjugated with proteins....
An anti-phenolphthalein monoclonal antibody (mAb) was prepared based on the N,N'-Carbonyldiimidazole (CDI) method through phenolphthalein conjugated with proteins. Indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold-based immunochromatographic assay (ICA) methods were used to determine phenolphthalein in slimming products. A standard curve was established, and the IC and limit of detection of ic-ELISA were 0.95 and 0.10 ng/mL with a linear detection range of 0.27-3.37 ng/mL. The developed ICA was used to detect phenolphthalein in tablets, capsules, and slimming tea samples with visual limit of detection values of 10 μg/kg, and cut-off values of 200 μg/kg. The results indicated that these two methods could be used to quickly detect phenolphthalein in slimming products.
Topics: Chromatography, Affinity; Enzyme-Linked Immunosorbent Assay; Gold Colloid; Immunoassay; Phenolphthalein
PubMed: 35158187
DOI: 10.1016/j.jpba.2022.114609 -
Environmental Health Perspectives Feb 1997
Topics: Animals; Birth Weight; Body Weight; Cathartics; Dose-Response Relationship, Drug; Epididymis; Female; Fertility; Litter Size; Male; Mice; Organ Size; Phenolphthalein; Phenolphthaleins; Pregnancy; Reproduction; Seminiferous Tubules; Sperm Count; Spermatozoa; Testis
PubMed: 9114350
DOI: No ID Found -
Journal of the American Pharmaceutical... Jul 1948
Topics: Humans; Phenolphthalein
PubMed: 18884019
DOI: 10.1002/jps.3030370703 -
Journal of Forensic Sciences Mar 2014Presumptive tests for blood play a critical role in the examination of physical evidence and in the determination of subsequent analysis. The catalytic power of...
Presumptive tests for blood play a critical role in the examination of physical evidence and in the determination of subsequent analysis. The catalytic power of hemoglobin allows colorimetric reactions employing phenolphthalein (Kastle-Meyer test) to indicate "whether" blood is present. Consequently, DNA profiles extracted from phenolphthalein-positive stains are presumed to be from blood on the evidentiary item and can lead to the identification of "whose" blood is present. Crushed nodules from a variety of legumes yielded phenolphthalein false-positive reactions that were indistinguishable from true bloodstains both in color quality and in developmental time frame. Clothing and other materials stained by nodules also yielded phenolphthalein false-positive reactivity for several years after nodule exposure. Nodules from leguminous plants contain a protein (leghemoglobin) which is structurally and functionally similar to hemoglobin. Testing of purified leghemoglobin confirmed this protein as a source of phenolphthalein reactivity. A scenario is presented showing how the presence of leghemoglobin from nodule staining can mislead investigators.
Topics: Blood Stains; Colorimetry; DNA; DNA Fingerprinting; Electrophoresis, Capillary; Fabaceae; False Positive Reactions; Hemoglobins; Humans; Leghemoglobin; Phenolphthalein; Polymerase Chain Reaction; Reagent Strips; Root Nodules, Plant
PubMed: 24313711
DOI: 10.1111/1556-4029.12352 -
Report on Carcinogens : Carcinogen... 2004
Topics: Animals; CHO Cells; Carcinogenicity Tests; Carcinogens; Cells, Cultured; Cricetinae; Cricetulus; Female; Government Regulation; Guidelines as Topic; Humans; Indicators and Reagents; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; Models, Biological; Occupational Exposure; Phenolphthalein; Rats; Rats, Inbred F344; United States
PubMed: 21089944
DOI: No ID Found -
Food Chemistry Feb 2021In this study, 3,3-bis(4-hydroxy-3-((E)-((4-hydroxyphenyl)imino)methyl) phenyl)isobenzofuran-1(3H)-one (HMBP) was designed as a ''turn-on″ fluorogenic chemosensor to...
In this study, 3,3-bis(4-hydroxy-3-((E)-((4-hydroxyphenyl)imino)methyl) phenyl)isobenzofuran-1(3H)-one (HMBP) was designed as a ''turn-on″ fluorogenic chemosensor to detect Al. Studies were performed in CHOH-HEPES (v/v, 9/1, pH 7.0) media at λ = 475 nm. The LOD value was found to be 0.113 µM. The stoichiometric ratio of HMBP-Al was determined as 1:2 by Job's plot and ESI-MS as well as H NMR titration. The binding constant of chemosensor HMBP with Al from the Benesi-Hildebrand equation was determined to be 1.21 × 10 M. The quantum (Φ) yields were obtained as 0.040 and 0.775 for the chemosensor HMBP and HMBP-Al, respectively. The response of the chemosensor HMBP towards Al was attributed to the strategies of blocking the photo-induced electron transfer (PET) and CN isomerisation mechanisms. Finally, the sensing of the chemosensor HMBP for the determination of Al in real food samples, drinking waters and herbal teas, were employed.
Topics: Aluminum; Drinking Water; Fluorescent Dyes; Food Analysis; Isomerism; Magnetic Resonance Spectroscopy; Phenolphthalein; Sensitivity and Specificity; Teas, Herbal
PubMed: 32781355
DOI: 10.1016/j.foodchem.2020.127659 -
Journal of the Indian Medical... Oct 2000
Topics: Cathartics; Humans; Phenolphthalein
PubMed: 11258497
DOI: No ID Found