-
Colloids and Surfaces. B, Biointerfaces Apr 2010Prior infrared spectroscopic studies of extracellular polymeric substances (EPS) and live bacterial cells have indicated that organic phosphate groups mediate cell...
Prior infrared spectroscopic studies of extracellular polymeric substances (EPS) and live bacterial cells have indicated that organic phosphate groups mediate cell adhesion to iron oxides via inner-sphere P-OFe surface complexation. Since cell membrane phospholipids are a potential source of organic phosphate groups, we investigated the adhesion of phospholipidic vesicles to the surfaces of the iron (oxyhydr)oxides goethite (alpha-FeOOH) and hematite (alpha-Fe2O3) using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. l-alpha-phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidic acid (PA) were used because they are vesicle forming phospholipids representative of prokaryotic and eukaryotic cell surface membranes. Phospholipid vesicles, formed in aqueous suspension, were characterized by transmission electron microscopy (TEM), multi-angle laser light scattering (MALS) and quasi-elastic light scattering (QELS). Their adhesion to goethite and hematite surfaces was studied with ATR-FTIR at pH 5. Results indicate that PC and PE adsorption is affected by electrostatic interaction and H-bonding (PE). Conversely, adsorption of PA involves phosphate inner-sphere complexes, for both goethite and hematite, via P-OFe bond formation. Biomolecule adsorption at the interface was observed to occur on the scale of minutes to hours. Exponential and linear increases in peak intensity were observed for goethite and hematite, respectively. Our ATR-FTIR results on the PA terminal phosphate are in good agreement with those on EPS reacted with goethite and on bacterial cell adhesion to hematite. These findings suggest that the plasma membrane, and the PA terminal phosphate in particular, may play a role in mediating the interaction between bacteria and iron oxide surfaces during initial stages of biofilm formation.
Topics: Ferric Compounds; Iron Compounds; Minerals; Particle Size; Phospholipids; Spectroscopy, Fourier Transform Infrared; Surface Properties
PubMed: 20074916
DOI: 10.1016/j.colsurfb.2009.12.005 -
Biochimica Et Biophysica Acta Apr 1977Cells of Escherichia coli were incubated in broth medium in the presence of 5 mM of hydroxylamine which completely inhibited growth but did not affect viabilities....
Cells of Escherichia coli were incubated in broth medium in the presence of 5 mM of hydroxylamine which completely inhibited growth but did not affect viabilities. Hydroxylamine is known to inhibit phosphatidylserine decarboxylase. A large amount of phosphatidylserine (up to 20% of total phospholipids), which did not occur in normal cells, accumulated accompanied with a decrease in phosphatidylethanolamine. Higher uptake activities of serine and glutamate were observed with the hydroxylamine-treated cells than control cells. When membrane vesicles from hydroxylamine-treated cells were prepared, they also displayed higher uptake activities of serine, proline, glutamate, and threonine than those of normal membranes. When hydroxylamine-treated cells were incubated with chloramphenicol, at concentrations which almost completely inhibited protein synthesis, the composition of phosphatidylserine decreased with a concomitant increase in that of phosphatidylethanolamine. The phospholipid composition of these cells incubated for 5 h with chloramphenicol became almost normal. Membranes vesicles prepared from such cells displayed reduced uptake activities, which were close to those of normal vesicles. These results were interpreted as indicating the altered transport activities due to the altered phospholipid composition.
Topics: Amino Acids; Biological Transport, Active; Cell Membrane; Escherichia coli; Hydroxylamines; Kinetics; Membrane Lipids; Phospholipids
PubMed: 322713
DOI: 10.1016/0005-2736(77)90207-3 -
Biophysical Journal Sep 1975
Topics: Calcium; Macromolecular Substances; Membranes, Artificial; Phosphatidylcholines; Phosphatidylglycerols; Phosphatidylserines; Phospholipids
PubMed: 1182269
DOI: 10.1016/S0006-3495(75)85872-3 -
Lipids May 1969
Topics: Animals; Anura; Cattle; Humans; Lung; Lysophosphatidylcholines; Mice; Phosphatidylcholines; Phosphatidylethanolamines; Phosphatidylinositols; Phospholipids; Rats; Species Specificity; Sphingomyelins
PubMed: 4306679
DOI: 10.1007/BF02532640 -
Journal of Chromatography. B,... Jun 2008A rapid and specific analytical method for simultaneous determination and quantification of seven major phospholipid classes in human blood was developed by normal-phase...
Simultaneous determination and quantification of seven major phospholipid classes in human blood using normal-phase liquid chromatography coupled with electrospray mass spectrometry and the application in diabetes nephropathy.
A rapid and specific analytical method for simultaneous determination and quantification of seven major phospholipid classes in human blood was developed by normal-phase high-performance liquid chromatography tandem mass spectrometry. The optimal separation was achieved by using mobile phase hexane (A) and 2-propanol with water, formic acid and ammonia as modifiers (B) using an HPLC diol column. Isocratic elution method was used for better repeatability and no balance time. The seven major phospholipid classes in human blood that were detected including phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI) phosphatidylcholine (PC), lysophosphatidylcholine (Lyso-PC), and sphingomyelin (SM). That can be separated in this condition. Every phospholipid class contains many molecular species which have similar structure. The structure of phospholipids molecular species was identified by ion-trap MS(n) which produced ion fragments. And the qualification was completed by TOF-MS which shows good accuracy. Through the accurate quantification of one representative phospholipids molecule in each class, a method for simultaneous estimation hundreds of molecular species in seven major classes was established. The intra-day and inter-day precision and recovery had been investigated in detail. The RSD of precision for most compound is below 8% and RE is below 10%. Recovery is almost over 80%. This method was applied to phospholipids disorder related with diabetes nephropathy successfully. The concentrations of most phospholipids for normal people are higher than that for diabetic nephropathy (DN) patients in three phases. For most of phospholipids, with the development of DN the concentration was decreasing.
Topics: Chromatography, High Pressure Liquid; Diabetic Nephropathies; Humans; Phospholipids; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry
PubMed: 18524699
DOI: 10.1016/j.jchromb.2008.05.027 -
Annales de Microbiologie Sep 1974
Topics: Bacillus subtilis; Cell Differentiation; Cell Division; Cell Membrane; Glycerol; Mutation; Phenotype; Phosphatidylethanolamines; Phospholipids; Phosphorus Radioisotopes; Spores, Bacterial
PubMed: 4218463
DOI: No ID Found -
Bioanalysis Apr 2012Several new products have been marketed with the specific capability of removing phospholipids. These products were evaluated alongside more traditional extraction...
BACKGROUND
Several new products have been marketed with the specific capability of removing phospholipids. These products were evaluated alongside more traditional extraction techniques, using UHPLC and precursor-ion scanning (pre m/z 184), which detects glycerophosphocholines (GPCho), lyso-GPCho and sphingomyelins. Using this technique the plasma GPCho profile of human, dog and rat plasma is briefly compared.
RESULTS
Precursor-ion scanning detected more of the phospholipid profile in extracts than a SRM experiment (m/z 184-184). Products designed for the purpose were the most efficient at removing phospholipids and reversed-phase SPE was better than mixed-mode cation exchange. A comparison of different UHPLC columns demonstrated that C(8) or phenyl phases would help manage the elution of GPChos.
CONCLUSION
Phospholipid removal plates are useful where no sample concentration is required; however for more challenging LOD it will be necessary to enrich the original sample. In these cases build-up of phospholipids can be avoided with a thoughtful choice of UHPLC column.
Topics: Analytic Sample Preparation Methods; Animals; Chemical Fractionation; Chemical Precipitation; Chromatography, High Pressure Liquid; Dogs; Humans; Hydrophobic and Hydrophilic Interactions; Liquid-Liquid Extraction; Phospholipids; Rats; Solid Phase Extraction
PubMed: 22512798
DOI: 10.4155/bio.12.38 -
Rapid Communications in Mass... 2005Direct-injection electrospray ionization mass spectrometry in combination with information-dependent data acquisition (IDA), using a triple-quadrupole/linear ion trap...
Direct-injection electrospray ionization mass spectrometry in combination with information-dependent data acquisition (IDA), using a triple-quadrupole/linear ion trap combination, allows high-throughput qualitative analysis of complex phospholipid species from child whole blood. In the IDA experiments, scans to detect specific head groups (precursor ion or neutral loss scans) were used as survey scans to detect phospholipid classes. An enhanced resolution scan was then used to confirm the mass assignments, and the enhanced product ion scan was implemented as a dependent scan to determine the composition of each phospholipid class. These survey and dependent scans were performed sequentially and repeated for the entire duration of analysis, thus providing the maximum information from a single injection. In this way, 50 different phospholipids belonging to the phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylcholine and sphingomyelin classes were identified in child whole blood.
Topics: Child; Chromatography, High Pressure Liquid; Humans; Molecular Structure; Phospholipids; Spectrometry, Mass, Electrospray Ionization
PubMed: 16059884
DOI: 10.1002/rcm.2080 -
Hoppe-Seyler's Zeitschrift Fur... Mar 1979In a previous publication, a new phospholipid was isolated from the keratinoidal layer of chicken gizzard, and on the basis of chemical, spectrometric (IR, NMR, UV)...
In a previous publication, a new phospholipid was isolated from the keratinoidal layer of chicken gizzard, and on the basis of chemical, spectrometric (IR, NMR, UV) tests and mass analysis, the probable structure of the isolated phospholipid was proposed as 1,2-O-docosylidene-sn-glycero-3-phospho(1'-ribosyl)-ethanolamine (IX). In this paper, on the basis of probable structure, a new phospholipid was synthetized.
Topics: Animals; Chickens; Gizzard, Avian; Magnetic Resonance Spectroscopy; Mass Spectrometry; Phospholipids; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet
PubMed: 437698
DOI: 10.1515/bchm2.1979.360.1.477 -
Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids.Biochimica Et Biophysica Acta Dec 1994We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles...
We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca(2+)-mediated vesicle fusion, confirmed this interpretation.
Topics: Dimyristoylphosphatidylcholine; Lipid Bilayers; Membranes; Phosphatidylglycerols; Phospholipids; Succinimides
PubMed: 7841187
DOI: 10.1016/0005-2736(94)00218-5