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Biochimica Et Biophysica Acta Sep 1977In an accompanying publication by Duckwitz-Peterlein, Eilenberger and Overath ((1977) Biochim. Biophys. Acta 469,311--325) it is shown that the exchange of lipid...
In an accompanying publication by Duckwitz-Peterlein, Eilenberger and Overath ((1977) Biochim. Biophys. Acta 469,311--325) it is shown that the exchange of lipid molecules between negatively charged vesicles consisting of total phospholipid extracts from Escherichia coli occurs by the transfer of single lipid monomers or small micelles through the water. Here a kinetic interpretation is presented in terms of a rate constant, k--, for the escape of lipid molecules from the vesicle bilayer into the water. The evaluated rate constants are kP- = (0.86 +/- 0.05) - 10(-5) S-1 and ke- = (1.09 +/- 0.13) - 10(-6) s-1 for phospholipid molecules with trans-delta 9-hexadecenoate and trans-delta 9-octadecenoate, respectively, as the predominant acyl chain component. The rate constants are discussed in terms of the acyl chain and polar head group composition of the lipids.
Topics: Cardiolipins; Escherichia coli; Fatty Acids; Membrane Lipids; Models, Biological; Phosphatidylethanolamines; Phosphatidylglycerols; Phospholipids; Water
PubMed: 332231
DOI: 10.1016/0005-2736(77)90168-7 -
Lipids Feb 1992Detailed analyses of the phospholipid compositions of cultured human endothelial cells are reported here. No significant differences were found between the phospholipid...
Detailed analyses of the phospholipid compositions of cultured human endothelial cells are reported here. No significant differences were found between the phospholipid compositions of cells from human artery, saphenous and umbilical vein. However, due to the small sample sizes, relatively large standard deviations for some of the phospholipid classes were observed. A representative composition of endothelial cells is: phosphatidylcholine 36.6%, choline plasmalogen 3.7%, phosphatidylethanolamine 10.2%, ethanolamine plasmalogen 7.6%, sphingomyelin 10.8%, phosphatidylserine 7.1%, lysophosphatidylcholine 7.5%, phosphatidylinositol 3.1%, lysophosphatidylethanolamine 3.6%, phosphatidylinositol 4,5-bisphosphate 1.8%, phosphatidic acid 1.9%, phosphatidylinositol 4-phosphate 1.5%, and cardiolipin 1.9%. The cells possess high choline plasmalogen and lysophosphatidylethanolamine contents. The other phospholipids are within the normal biological ranges expected. Phospholipids were separated by high-performance liquid chromatography and quantified by lipid phosphorus assay.
Topics: Cell Extracts; Cells, Cultured; Chromatography, High Pressure Liquid; Endothelium, Vascular; Humans; Phosphatidylinositol Phosphates; Phosphatidylinositols; Phospholipids; Plasmalogens
PubMed: 1315902
DOI: 10.1007/BF02535816 -
Food Chemistry Feb 2021During dry-cured ham processing, phospholipids (PL) are the main substrates of lipolysis and oxidation. However, the published data on individual PL classes in the pig...
During dry-cured ham processing, phospholipids (PL) are the main substrates of lipolysis and oxidation. However, the published data on individual PL classes in the pig muscle are inconsistent. This study determined the PL class contents and composition in biceps femoris of Iberian pigs according to the rearing system (Montanera vs Pienso). Phosphatidylcholine (PC), phosphatidylethanolamine (PE), cardiolipin (CL) and sphingomyelin contents were higher in the Montanera pigs than in the Pienso pigs. Total PL and PC, PE, phosphatidylinositol + phosphatidylserine and CL contained higher levels of oleic acid and slightly higher levels of n-3 polyunsaturated fatty acids (PUFA) but lower levels of n-6 PUFA in the Montanera pigs than in the Pienso pigs. The rearing system had no effect on the plasmalogen content but influenced aldehyde composition, mainly in PE and in total PL. These results can partially explain the differences in sensory properties of dry-cured ham between the Montanera and Pienso systems.
Topics: Animals; Hamstring Muscles; Humans; Oxidation-Reduction; Phospholipids; Swine
PubMed: 33091977
DOI: 10.1016/j.foodchem.2020.128102 -
Journal of Lipid Research Apr 1994In recent studies we showed that the phospholipid analogue hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis by affecting the translocation of the...
In recent studies we showed that the phospholipid analogue hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis by affecting the translocation of the rate-limiting enzyme of phosphatidylcholine biosynthesis, CTP:phosphocholine cytidylyltransferase (EC 2.7.7.15), to membranes, where it is active (Geilen et al. 1992. J. Biol. Chem. 267: 6719-6724). The present study was performed to investigate the structure-dependency of this effect. It is shown that the inhibitory properties of phospholipid analogues are dependent on their alkyl side chain length (dodecylphosphocholine < tetradecylphosphocholine < hexadecylphosphocholine < heptadecylphosphocholine < octadecylphosphocholine > eicosadecylphosphocholine). Furthermore, it is demonstrated that this inhibition of phosphatidylcholine biosynthesis by phospholipid analogues is also dependent on the polar head group (hexadecylphosphocholine >> hexadecylphosphoethanolamine = hexadecylphosphoserine). These effects result from an inhibition of the CTP:phosphocholine cytidylyltransferase and are not due to an inhibition of choline uptake or differences in the cellular uptake of the phospholipid analogues investigated.
Topics: Animals; Cell Division; Cell Line; Cells, Cultured; Choline-Phosphate Cytidylyltransferase; Digitonin; Dogs; Kidney; Nucleotidyltransferases; Phosphatidylcholines; Phospholipids; Structure-Activity Relationship; Tetradecanoylphorbol Acetate
PubMed: 8006517
DOI: No ID Found -
Zhongguo Zhong Yao Za Zhi = Zhongguo... Jul 2013According to Chinese and foreign literatures and reports in recent years, this article introduced the latest advance in studies on phospholipid compound of traditional... (Review)
Review
According to Chinese and foreign literatures and reports in recent years, this article introduced the latest advance in studies on phospholipid compound of traditional Chinese medicines in terms of its preparation mechanism, preparation process, characterization and transmembrane absorption. Under appropriate conditions, traditional Chinese medicines could generate phospholipid compound, whose physico-chemical property differs from the original drug, with a better absorption and improved bioavailability. Therefore, there is huge room for further study and development of phospholipid compound with traditional Chinese medicines.
Topics: Absorption; Animals; Biological Availability; Humans; Medicine, Chinese Traditional; Phospholipids
PubMed: 24079223
DOI: No ID Found -
Bulletin de La Societe Des Sciences... 1987
Topics: Autoantibodies; Cardiolipins; Diagnosis, Differential; Humans; Lupus Erythematosus, Systemic; Phospholipids; Syphilis Serodiagnosis
PubMed: 3664905
DOI: No ID Found -
Presse Medicale (Paris, France : 1983) Mar 1986
Topics: Abortion, Habitual; Autoantibodies; Female; Humans; Immunoglobulins; Phospholipids; Pregnancy; Syphilis Serodiagnosis; Thrombosis
PubMed: 2938103
DOI: No ID Found -
Lipids Sep 1998The phospholipid composition was determined for the amebocyte of the primitive arthropod Limulus polyphemus. The total fatty acid composition of the cells' lipids was...
The phospholipid composition was determined for the amebocyte of the primitive arthropod Limulus polyphemus. The total fatty acid composition of the cells' lipids was analyzed by gas chromatography/mass spectrometry (GC/MS) of fatty acid methyl esters (FAME). The FAME analysis revealed high levels of 20-carbon polyunsaturated fatty acids (PUFA), especially arachidonic (20:4n-6) and eicosapentaenoic (20:5n-3) acids. Almost 20% of the total lipid profile was comprised of dimethyl acetals of 16- to 20-carbon chain lengths, indicative of plasmalogens in the phospholipid pool. Phospholipids, analyzed by high-pressure liquid chromatography, included phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI), sphingomyelin (SPH), and cardiolipin (CL). PE and PC levels predominated at 42.2 and 36.3%, respectively. Smaller amounts of PS (9.0%) and PI (6.2%) were present, as well as low levels of SPH (4.6%), CL (1.6%), and trace amounts of lysophosphatidylcholine. The major phospholipid species, PE, PC, PS and PI, were collected and their molecular species were examined by electrospray-ionization mass spectrometry. The molecular species within the phospholipid classes reflected the high levels of PUFA seen in the total lipid profile. PI was mainly composed of 18:0a/20:4. Over half of the PS consisted of 18:0a/18:1 and 18:0a/20:4. The major PE species were 20:1p/20:5, 20:1p/20:4, 18:0p/20:5, and 18:0p/20:4. PC had the largest distribution of molecular species, and its most abundant species were 16:0e/20:5, 16:0e/20:4, and 16:0p/20:4. The presence of 16:0e/20:4 is the first documentation of a specific precursor to platelet-activating factor in an invertebrate hemocyte. Note: at the sn-1 position: [a=1=O-acyl, e = 1-O-alkylether, and p = 1-O-alk-1'-enyl (plasmalogen)].
Topics: Animals; Cardiolipins; Chromatography, Gas; Chromatography, High Pressure Liquid; Fatty Acids; Horseshoe Crabs; Lysophosphatidylcholines; Mass Spectrometry; Phospholipids; Sphingomyelins
PubMed: 9778141
DOI: 10.1007/s11745-998-0290-y -
Advances in Microbial Physiology 1978
Review
Topics: Bacteria; Bacteriophages; Chemical Phenomena; Chemistry; Enzyme Activation; Membrane Lipids; Phospholipases; Phospholipids
PubMed: 380310
DOI: 10.1016/s0065-2911(08)60417-8 -
PloS One 2013Phospholipid membranes are thought to be one of the main inducers of hemozoin formation in Plasmodia and other blood-feeding parasites. The "membrane surrounding...
Phospholipid membranes are thought to be one of the main inducers of hemozoin formation in Plasmodia and other blood-feeding parasites. The "membrane surrounding hemozoin" has been observed in infected cells but has not been observed in in vitro experiments. This study focused on observing the association of phospholipid membranes and synthetic β-hematin, which is chemically identical to hemozoin, and on a further exploration into the mechanism of phospholipid membrane-induced β-hematin formation. Our results showed that β-hematin formation was induced by phospholipids in the fluid phase but not in the gel phase. The ability of phospholipids to induce β-hematin formation was inversely correlated with gel-to-liquid phase transition temperatures, suggesting an essential insertion of heme into the hydrocarbon chains of the phospholipid membrane to form β-hematin. For this study, a cryogenic transmission electron microscope was used to achieve the first direct observation of the formation of a monolayer of phospholipid membrane surrounding β-hematin.
Topics: Hemeproteins; Membranes; Microscopy, Electron, Transmission; Phospholipids; Plasmodium
PubMed: 23894579
DOI: 10.1371/journal.pone.0070025