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Journal of Reproduction and Fertility Mar 1985A significant increase in total phospholipid content of the endometrium took place during the secretory phase of the human menstrual cycle (26% increase from...
A significant increase in total phospholipid content of the endometrium took place during the secretory phase of the human menstrual cycle (26% increase from mid-proliferative to premenstrual stage). The major phospholipid, phosphatidylcholine, was increased by 30%, whereas phosphatidylethanolamine was unchanged. Phosphatidyl-serine and -inositol underwent the largest percentage increases (40%). Phosphatidic acid levels were the only ones to decrease (-52%), a finding consistent with the role of this lipid as precursor of the increased phospholipids. The changes did not markedly affect phospholipid composition, except for a significant decrease in the proportions of phosphatidate and phosphatidylethanolamine. Arachidonate and eicosatrienoate (n-6) were the major polyunsaturated fatty acids. C22 tetra-, penta- and hexa-enoic fatty acids of the n-3 and n-4 families were also present in all major endometrial glycerophospholipids throughout the cycle. The mass changes in phospholipids during the cycle occurred without alteration of their fatty acid composition.
Topics: Adult; Endometrium; Fatty Acids; Female; Humans; Menstrual Cycle; Phosphatidic Acids; Phosphatidylcholines; Phosphatidylethanolamines; Phosphatidylinositols; Phosphatidylserines; Phospholipids
PubMed: 3989789
DOI: 10.1530/jrf.0.0730317 -
Langmuir : the ACS Journal of Surfaces... Sep 2011In this article, we designed and synthesized an amino-functionalized hybrid hydrocarbon/fluorocarbon double-chain phospholipid (ACFPC) containing one chain with the...
In this article, we designed and synthesized an amino-functionalized hybrid hydrocarbon/fluorocarbon double-chain phospholipid (ACFPC) containing one chain with the hydrophobic fluorocarbon chain and terminal amino, amide, and ether linkages and one chain with the hydrocarbon chain. The novel reactive phospholipid was fully characterized with Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), and mass spectrometry (MS). Then the self-assembly behaviors of the hybrid double-chain phospholipid in aqueous and acidic media were investigated with transmission electron microscopy (TEM), the critical micelle concentration (cmc), dynamic light scattering (DLS), and the hydrocarbon double-chain phospholipid (ACCPC) for comparison. Moreover, their self-assembled structures in aqueous and acidic media were simulated using the dissipative particle dynamics (DPD) method. These results suggest that the fluorocarbon/hydrocarbon hybrid-chain phospholipid can self-assemble into a more stable microstructure compared to the double hydrocarbon chain phospholipid, which will have the potential ability to self-assemble into a more stable minicking biomembrane structure onto material surfaces to inhibit protein adsorption under complicated physiological conditions.
Topics: Adsorption; Amines; Hydrocarbons; Models, Molecular; Molecular Structure; Particle Size; Phospholipids; Surface Properties
PubMed: 21682339
DOI: 10.1021/la201610w -
Analytical and Bioanalytical Chemistry Feb 2016Phospholipid quantification in biological samples is crucial and is increasingly studied in lipidomics. Quantitative studies are often performed using commercially...
Phospholipid quantification in biological samples is crucial and is increasingly studied in lipidomics. Quantitative studies are often performed using commercially available standards of phospholipid classes in order to mimic the composition of biological samples. For this, studies are conducted by liquid chromatography coupled to electrospray ionization-mass spectrometry. In liquid chromatography coupled to mass spectrometry (LC-MS) analysis, the matrix components and the co-elution of several phospholipid species lead to the phenomenon of ion suppression. As a result, a decrease in the response of phospholipid species in mass spectrometry MS is observed. In fact, inter-species ion suppression affects the efficiency of phospholipid (PL) ionization and might also influence the quantitative results. The aim of this work is to study the PL inter-species ion suppression phenomenon in electrospray ionization (ESI)-mass spectrometry on a triple quadrupole TQ and an LTQ-Orbitrap in order to improve quantification in natural and biological samples. Thus, the phospholipid MS response was evaluated to study the effect of acyl chain length, the degree, and the position of unsaturation on acyl chain and the effect of the polar head group structure. A number of saturated and unsaturated phospholipid species and mixtures were analyzed in different ionization modes to a better understanding of inter-species ion suppression phenomenon. PL molecular species responded differently according to the length of fatty acid chains, the number of unsaturation, and the nature of the polar head group. Fatty acid chain length showed to have the most marked effect on MS response.
Topics: Chromatography, Liquid; Humans; Phospholipids; Spectrometry, Mass, Electrospray Ionization
PubMed: 26780707
DOI: 10.1007/s00216-015-9245-6 -
Analytical Chemistry May 1991When phospholipids ionized by fast atom bombardment undergo collisionally induced dissociation (CID), they cleave at specific bonds between the functional groups...
When phospholipids ionized by fast atom bombardment undergo collisionally induced dissociation (CID), they cleave at specific bonds between the functional groups contained on the lipid. These cleavages are common to all classes of phospholipids. By taking advantage of this fact, a general scheme has been developed that uses a triple-quadrupole mass spectrometer to rapidly characterize the phospholipid content and structures present in crude lipid extracts. This scheme is based on fast atom bombardment ionization of a crude lipid extract and on the combination of positive-ion neutral-loss and parent scans and negative-ion daughter scans. Neutral-loss and parent scans provide independent diagnostic mass spectra for each of many specific phospholipid classes, while daughter scans provide the emperical formulas and positions of the fatty acyl constituents on each phospholipid. An automated tandem mass spectrometry (MS/MS) instrument can perform an extensive phospholipid screening on a single sample. A useful mass profile of the phosphatidylethanolamine species present in a 1-pg sample of mixed phospholipids (equivalent to ten Escherichia coli cells) has been obtained. The spectra are reproducible and proportional to concentration over at least the five-logarithm range of cell concentrations studied. A rapid extraction procedure combined with the automated instrument control program produces profiles of the phospholipid classes, along with fatty acyl empirical formulas and position information, on selected phospholipid species, in a few minutes, from a single sample.
Topics: Bacteria; Phospholipids; Spectrometry, Mass, Fast Atom Bombardment
PubMed: 1872477
DOI: 10.1021/ac00010a020 -
Omics : a Journal of Integrative Biology Dec 2010By employing electrospray ionization tandem mass spectrometry (ESI-MS/MS), the phospholipidomes of eight hemiascomycetous human pathogenic Candida species have been...
By employing electrospray ionization tandem mass spectrometry (ESI-MS/MS), the phospholipidomes of eight hemiascomycetous human pathogenic Candida species have been characterized. Over 200 phospholipid molecular species were identified and quantified. There were no large differences among Candida species in phosphoglyceride class composition; however, differences in phosphoglycerides components (i.e., fatty acyl chains) were identified. In contrast, differences in sphingolipid class composition as well as in molecular species were quite evident. The phospholipid compositions of C. albicans, C. glabrata, C. parapsilosis, C. kefyr, C. tropicalis, C. dubliniensis, C. krusei, and C. utilis could be further discriminated by principal component analysis. Notwithstanding that a single strain of each species was analyzed, our data do point to a typical molecular species imprint of Candida strains.
Topics: Candida; Phospholipids; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry
PubMed: 20726778
DOI: 10.1089/omi.2010.0041 -
Clinical Chemistry May 1989In prevention of the respiratory distress syndrome (RDS), measurements of surface tension values (a biophysical property) of amniotic-fluid samples are correlated with... (Comparative Study)
Comparative Study
In prevention of the respiratory distress syndrome (RDS), measurements of surface tension values (a biophysical property) of amniotic-fluid samples are correlated with their lecithin/sphingomyelin (L/S) ratios (a biochemical property). According to some authors, precipitation of phospholipid with cold acetone is essential for determining the L/S ratio, because it separates surfactant and nonsurfactant phospholipidic fractions. Here we report the first study of the ability of three amniotic-fluid components to decrease surface tension: The complete lipid extract (without precipitation), and the fractions precipitated and (or) remaining soluble after addition of cold acetone. Addition of increasing aliquots of lipid extracts to these three samples showed that: (a) measurement of surface tension rapidly and reliably indicates fetal lung maturity, and (b) both precipitated and soluble phospholipid fractions decrease surface tension similarly, making it unlikely that the precipitation step in fact separates surfactant and nonsurfactant material.
Topics: Acetone; Amniotic Fluid; Chemical Precipitation; Cold Temperature; Female; Humans; Infant, Newborn; Phosphatidylcholines; Phospholipids; Pregnancy; Prenatal Diagnosis; Pulmonary Surfactants; Respiratory Distress Syndrome, Newborn; Sphingomyelins; Surface Tension; Tissue Extracts
PubMed: 2720973
DOI: No ID Found -
Cellular & Molecular Biology Letters 2005The aim of this investigation was to characterize the phospholipid composition of normal human blood mononuclear cells using 31P NMR spectroscopy. Mononuclear cells of... (Comparative Study)
Comparative Study
The aim of this investigation was to characterize the phospholipid composition of normal human blood mononuclear cells using 31P NMR spectroscopy. Mononuclear cells of peripheral blood were obtained from 10 volunteers. Phospholipid extracts were prepared from 60x10(6) cells according to modified Folch's method. An AMX 300 Bruker spectrometer 7.05 T was used. The 31P spectrum of phospholipid extracts from normal human PBMC consisted of 9 peaks, with one each for phosphatidylcholine (PC), plasmalogen of phosphatidylcholine (CPLAS), lysophosphatidylcholine (LPC), sphingomyelin (SM), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS) and cardiolipin (CL), and another one due to the external reference substance, methylenediphosphonic acid (MDPA). The concentrations of these phospholipids (PL), based on the integral intensities, were as follows: 0.398 +/- 0.078 mmole/l for PC; 0.033 +/- 0.019 mmole/l for CPLAS; 0.155 +/- 0.043 mmole/l for SM; 0.266 +/- 0.104 mmole/l for PI+PE; 0.101 +/- 0.040 mmole/l for PS, and 0.026 +/- 0.033 mmole/l for CL. The results of this study confirmed that 31P MRS is a convenient tool for measuring the phospholipid concentrations of biological samples.
Topics: Adult; Cardiolipins; Female; Humans; Leukocytes, Mononuclear; Lysophosphatidylcholines; Male; Middle Aged; Nuclear Magnetic Resonance, Biomolecular; Phosphatidylcholines; Phosphatidylethanolamines; Phosphatidylinositols; Phosphatidylserines; Phospholipids; Phosphorus Isotopes; Plasmalogens; Sphingomyelins
PubMed: 16217549
DOI: No ID Found -
Chemical Communications (Cambridge,... Feb 2017Nanoscale chemical mapping of newly-synthesised phospholipid molecules inside a mammalian cell is demonstrated using tip-enhanced Raman spectroscopy (TERS) for the first...
Nanoscale chemical mapping of newly-synthesised phospholipid molecules inside a mammalian cell is demonstrated using tip-enhanced Raman spectroscopy (TERS) for the first time using mouse pre-adipocyte cells as a model system. Newly-synthesised membrane phospholipid distribution within a pre-adipocyte cell is mapped with <20 nm spatial resolution, overcoming the diffraction limit of confocal Raman spectroscopy via plasmonic enhancement of Raman signals at a TERS tip-apex.
Topics: Adipocytes; Animals; Deuterium; Intracellular Membranes; Mice; Phosphatidylcholines; Phospholipids; Spectrum Analysis, Raman
PubMed: 28177338
DOI: 10.1039/c6cc10226c -
Experientia Nov 1978The phospholipid composition of Dipylidium caninum has been studied. Chloroform-methanol-soluble fraction amounted to 2.4% and phospholipids to 0.5% of the wet weight of...
The phospholipid composition of Dipylidium caninum has been studied. Chloroform-methanol-soluble fraction amounted to 2.4% and phospholipids to 0.5% of the wet weight of the parasite. Phosphatidyl choline and phosphatidyl ethanolamine represented the bulk of the phospholipids, whereas phosphatidyl serine, phosphatidyl inositol, lysolecithin and lysophosphatidyl ethanolamine were present in minor amounts. Sulfatides were also identified in this parasite.
Topics: Animals; Cestoda; Dogs; Phosphatidylcholines; Phosphatidylethanolamines; Phosphatidylinositols; Phosphatidylserines; Phospholipids; Sulfoglycosphingolipids
PubMed: 569068
DOI: 10.1007/BF01932350 -
Sabouraudia Jul 1977Phospholipid synthesis, interconversion and breakdown in T. rubrum were followed by radioactive tracer. Synthesis and catabolism of phosphatidyl ethanolamine and...
Phospholipid synthesis, interconversion and breakdown in T. rubrum were followed by radioactive tracer. Synthesis and catabolism of phosphatidyl ethanolamine and phosphatidyl serine are most rapid; phosphatidyl choline and phosphatidyl inositol are metabolised rather slowly. Catabolism of phosphatidyl ethanolamine and phosphatidyl serine are uniform and their conversions to phosphatidyl choline and phosphatidyl inositol are suspected.
Topics: Phosphatidylcholines; Phosphatidylethanolamines; Phosphatidylglycerols; Phosphatidylinositols; Phosphatidylserines; Phospholipids; Trichophyton
PubMed: 905923
DOI: No ID Found