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Japanese Journal of Ophthalmology Jan 2017A slit-lamp examination is an indispensable and essential clinical evaluation method in ophthalmology, but, it is qualitative subjective. To complement its weaknesses in... (Review)
Review
A slit-lamp examination is an indispensable and essential clinical evaluation method in ophthalmology, but, it is qualitative subjective. To complement its weaknesses in making a quantitative evaluation of flare intensity and number of cells in the aqueous humor in the eye, we invented the laser flare-cell photometer in 1988. The instrument enables a non-invasive quantitative evaluation of flare intensity and number of cells in the aqueous with good accuracy and repeatability as well as maneuverability equal to slit-lamp microscopy. The instrument can elucidate the pathophysiology in the blood-aqueous barrier (BAB) function in a variety of ocular disorders. The accuracy of the instrument makes it possible to investigate not only the pathophysiology of intraocular disorders but also the effects of various drugs and surgical procedures in BAB. The instrument does not only lighten the burden on patients in clinical examinations and study but it also helps minimize the sacrifice of experimental animals and improves the reliability of the results by minimizing inter-individual variations through its good repeatability. Here I shall relate how the instrument has been applied to clinical and basic studies in ophthalmology and what novel knowledge its application contributed to pathophysiology in ophthalmology.
Topics: Animals; Aqueous Humor; Humans; Lasers; Ophthalmology; Photometry; Reproducibility of Results
PubMed: 27888376
DOI: 10.1007/s10384-016-0488-3 -
Science (New York, N.Y.) May 1883
PubMed: 17808075
DOI: 10.1126/science.ns-1.16.450 -
Canadian Journal of Research Oct 1948
Topics: Photometry
PubMed: 18103097
DOI: 10.1139/cjr48f-043 -
Science (New York, N.Y.) Aug 1975
PubMed: 17838768
DOI: 10.1126/science.189.4203.658 -
The Review of Scientific Instruments Sep 1950
Topics: Photometry
PubMed: 14786533
DOI: No ID Found -
The Biochemical Journal 1949
Topics: Photometry
PubMed: 15394430
DOI: No ID Found -
Scientific Reports Apr 2023This paper reports on developing a low cost but efficient paired emitter-detector diode (PEDD)-based photometer. The photometer consists of a white light-emitting diode...
This paper reports on developing a low cost but efficient paired emitter-detector diode (PEDD)-based photometer. The photometer consists of a white light-emitting diode (LED) as the emitter diode, an RGB LED as the detector diode, and a multimeter for recoding the signal. The developed PEDD-based photometer was utilized for the determination of liquid bleach adulteration in cow milk samples. N,N-Diethyl-p-phenylenediamine sulfate aqueous solution of pH 6 was used as a probe to monitor the presence of residual active chlorine in milk. The results showed that the developed method could be used to determine sodium hypochlorite in the concentration range of 0.5 to 20.0 ppm Cl with 0.14 and 0.46 ppm Cl limit of detection and limit of quantification, respectively. The intraday and interday precisions of the method at two concentration levels of 5.5 and 13.7 ppm Cl were 1.04% and 0.52%, and 1.81% and 1.02%, respectively. The recoveries of 114.2% and 106.9% were obtained for 5.5 and 13.7 ppm Cl concentrations levels, respectively. Real sample analyzes results showed that "maybe" liquid bleach adulteration in milk is the case for local distributors of raw milk.
Topics: Animals; Milk; Sodium Hypochlorite; Photometry
PubMed: 37069217
DOI: 10.1038/s41598-023-33527-y -
Il Farmaco; Edizione Pratica Jan 1954
Topics: Chemistry Techniques, Analytical; Photometry
PubMed: 13151202
DOI: No ID Found -
RSC Advances Apr 2023The demand for the development of portable and low-cost analytical devices has encouraged studies employing additive manufacturing techniques, such as 3D-printing. This...
The demand for the development of portable and low-cost analytical devices has encouraged studies employing additive manufacturing techniques, such as 3D-printing. This method can be used to produce components such as printed electrodes, photometers, and fluorometers for low-cost systems that provide advantages including low sample volume, reduced chemical waste, and easy coupling with LED-based optics and other instrumental devices. In the present work, a modular 3D-printed fluorometer/photometer was designed and applied for the determination of caffeine (CAF), ciprofloxacin (CIP), and Fe(ii) in pharmaceutical samples. All the plastic parts were printed separately by a 3D printer, using Tritan as the plastic material (black color). The final size of the modular 3D-printed device was 12 × 8 cm. The radiation sources were light-emitting diodes (LEDs), while a light dependent resistor (LDR) was used as a photodetector. The analytical curves obtained for the device were: = 3.00 × 10 [CAF] + 1.00 and = 0.987 for caffeine; = 6.90 × 10 [CIP] - 3.39 × 10 and = 0.991 for ciprofloxacin; and = 1.12 × 10 [Fe(ii)] + 1.26 × 10 and = 0.998 for iron(ii). The results obtained using the developed device were compared with reference methods, with no statistically significant differences observed. The 3D-printed device was composed of moveable parts, providing flexibility for adaptation and application as a photometer or fluorometer, by only switching the photodetector position. The LED could also be easily switched, permitting application of the device for different purposes. The cost of the device, including the printing and electronic components, was lower than US$10. The use of 3D-printing enables the development of portable instruments for use in remote locations with a lack of research resources.
PubMed: 37077256
DOI: 10.1039/d3ra01281f -
The Review of Scientific Instruments Aug 1950
Topics: Equipment and Supplies; Photometry
PubMed: 14781458
DOI: No ID Found