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Scientific Reports Jan 2022Psuedopregnancy for embryo transfer (ET) is usually induced in rats by mating with vasectomized males. Previously, we successfully induced pseudopregnancy using sonic...
Psuedopregnancy for embryo transfer (ET) is usually induced in rats by mating with vasectomized males. Previously, we successfully induced pseudopregnancy using sonic vibration instead (Easy-ET method). The transferred embryos developed normally. Conventionally, stimulation is performed 7 × 30 s with 5 min intervals at the day before ET. However, this protocol is time-consuming because it imitates natural mating behavior. Here, we investigated pseudopregnancy induction with shorter stimulation times. Stimulation was performed 2 × 30 s, with 30 s intervals at the proestrus stage at the day before ET. Of the transferred pronuclear or two-cell embryos, 43% or 62% developed normally, respectively. Furthermore, 67% or 68% of transferred pronuclear or two-cell embryos in rats at estrus stage stimulated on the day of ET developed normally, respectively. Pseudopregnancy was successfully induced with shorter stimulation. Furthermore, this protocol may be used to perform a single-day stimulation and ET operation at the estrus stage.
Topics: Animals; Embryo Transfer; Female; Male; Pregnancy; Pseudopregnancy; Rats; Sound
PubMed: 35075219
DOI: 10.1038/s41598-022-05293-w -
Journal of Reproduction and Fertility.... 1993Puberty in the female cat occurs between the ages of 8 and 10 months. Cats are seasonally polyoestrous, reflex ovulators. The oestrous cycle can occur as often as every... (Review)
Review
Puberty in the female cat occurs between the ages of 8 and 10 months. Cats are seasonally polyoestrous, reflex ovulators. The oestrous cycle can occur as often as every 2-3 weeks. Ovulation usually occurs 24-36 h after copulation, and implantation occurs 12-13 days after copulation. The duration of gestation in the cat is 64-67 days (average 66 days). The corpora lutea secrete increasing amounts of progesterone, starting 1-2 days after ovulation. If implantation occurs, progesterone concentrations continue to increase throughout days 25-30, then slowly decline throughout the rest of pregnancy. In the absence of pregnancy, the corpora lutea reach their peak progestational activity within 10-15 days and then decline, with basal progesterone values being noted by days 30-35. Relaxin is produced by the fetoplacental unit beginning at about day 20 of gestation and continuing throughout the rest of pregnancy. Prolactin production increases from about day 35. Like those of prostaglandin F2 alpha, concentrations of prolactin plateau at about day 50 and increase abruptly just before delivery.
Topics: Animals; Cat Diseases; Cats; Female; Hormones; Ovary; Pregnancy; Pseudopregnancy; Reproduction
PubMed: 8229938
DOI: No ID Found -
A.M.A. Archives of Neurology and... Jan 1951
Topics: Biomedical Research; Delusions; Female; Humans; Pseudopregnancy
PubMed: 14782782
DOI: No ID Found -
Psychosomatics Jul 1984
Topics: Adult; Fathers; Female; Humans; Male; Pseudopregnancy; Referral and Consultation; Sterilization, Tubal
PubMed: 6463193
DOI: 10.1016/s0033-3182(84)73010-6 -
Reproductive Sciences (Thousand Oaks,... Jan 2021It has been well established that uterine function during the peri-implantation period is precisely regulated by ovarian estrogen and progesterone. The embryo enters the...
It has been well established that uterine function during the peri-implantation period is precisely regulated by ovarian estrogen and progesterone. The embryo enters the uterine cavity before implantation. However, the impact of pre-implantation embryo on uterine function is largely unknown. In the present study, we performed RNA-seq analysis of mouse uterus on day 4 morning of natural pregnancy (with embryos in the uterus) and pseudo-pregnancy (without embryos in the uterus). We found that 146 genes were upregulated, and 77 genes were downregulated by the pre-implantation embryo. Gene ontology and gene network analysis highlighted the activation of inflammatory reaction in the uterus. By examining the promoter region of differentially expressed genes, we found that NF-kappaB was a causal transcription factor. Finally, we validated 4 inflammation-related genes by quantitative RT-PCR. These 4 genes are likely the main mediators of the inflammatory reaction in the uterus triggered by the pre-implantation embryo. Our data indicated that the pre-implantation embryo causes uterine inflammatory reaction, which in turn might contribute to the establishment of uterine receptivity and embryo implantation.
Topics: Animals; Blastocyst; Chemokines, CXC; Cyclooxygenase 2; Embryo Implantation; Female; Gene Regulatory Networks; Inflammation Mediators; Interleukins; Mice; NF-kappa B; Pregnancy; Pseudopregnancy; RNA-Seq; Uterus
PubMed: 32651899
DOI: 10.1007/s43032-020-00259-7 -
Psychosomatic Medicine 1960
Topics: Delusions; Female; Humans; Pseudopregnancy
PubMed: 13829165
DOI: 10.1097/00006842-196001000-00004 -
Wisconsin Medical Journal Nov 1958
Topics: Analgesics; Delusions; Female; Humans; Pseudopregnancy; Unconsciousness
PubMed: 13604319
DOI: No ID Found -
Psychosomatics 1991
Topics: Adult; Capgras Syndrome; Delusions; Female; Gender Identity; Humans; Male; Pseudopregnancy
PubMed: 2027947
DOI: 10.1016/s0033-3182(91)72097-5 -
Journal of Obstetrics and Gynaecology :... Apr 2007
Topics: Abdominal Pain; Adolescent; Contraceptives, Oral, Combined; Diagnosis, Differential; Ethinyl Estradiol-Norgestrel Combination; Female; Humans; Medroxyprogesterone Acetate; Pseudopregnancy
PubMed: 17464828
DOI: 10.1080/01443610701269119 -
The Nurse Practitioner Feb 1990Pseudocyesis is a condition in which an individual has the symptoms of true pregnancy and believes that he or she is pregnant, but pregnancy does not exist. Pseudocyesis...
Pseudocyesis is a condition in which an individual has the symptoms of true pregnancy and believes that he or she is pregnant, but pregnancy does not exist. Pseudocyesis is not confined to one nationality, race, social class or sex, and clinicians may encounter this condition in practice. Physiologic, psychodynamic and social components of pseudocyesis are described. A case study is presented that illustrates the correlation between pseudocyesis and childhood sexual abuse. The importance of accurate assessment of childhood sexual abuse in successful resolution of the condition is emphasized.
Topics: Adolescent; Child Abuse, Sexual; Female; Humans; Nursing Assessment; Pseudopregnancy
PubMed: 2308709
DOI: No ID Found