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Biofouling 2012Superhydrophobic titanium surfaces fabricated by femtosecond laser ablation to mimic the structure of lotus leaves were assessed for their ability to retain coccoid...
Superhydrophobic titanium surfaces fabricated by femtosecond laser ablation to mimic the structure of lotus leaves were assessed for their ability to retain coccoid bacteria. Staphylococcus aureus CIP 65.8T, S. aureus ATCC 25923, S. epidermidis ATCC 14990T and Planococcus maritimus KMM 3738 were retained by the surface, to varying degrees. However, each strain was found to preferentially attach to the crevices located between the microscale surface features. The upper regions of the microscale features remained essentially cell-free. It was hypothesised that air entrapped by the topographical features inhibited contact between the cells and the titanium substratum. Synchrotron SAXS revealed that even after immersion for 50 min, nano-sized air bubbles covered 45% of the titanium surface. After 1 h the number of cells of S. aureus CIP 65.8T attached to the lotus-like titanium increased to 1.27×10(5) mm(-2), coinciding with the replacement of trapped air by the incubation medium.
Topics: Air; Bacterial Adhesion; Gram-Positive Cocci; Hydrophobic and Hydrophilic Interactions; Lotus; Plant Leaves; Spectrum Analysis, Raman; Surface Properties; Titanium
PubMed: 22686938
DOI: 10.1080/08927014.2012.694426 -
Acta Crystallographica. Section F,... Aug 2011β-Galactosidases catalyze the hydrolysis of a galactosyl moiety from the nonreducing termini of oligosaccharides or from glycosides. A novel GH family 42 cold-active...
β-Galactosidases catalyze the hydrolysis of a galactosyl moiety from the nonreducing termini of oligosaccharides or from glycosides. A novel GH family 42 cold-active β-galactosidase identified from the psychrotrophic and halotolerant Planococcus sp. L4 (BgaP) was crystallized and a complete data set was collected from a single frozen crystal on an in-house X-ray source. The crystal diffracted to 2.8 Å resolution and belonged to space group P1, with unit-cell parameters a = 104.29, b = 118.12, c = 121.12 Å, α = 62.66, β = 69.48, γ = 70.74°. A likely Matthews coefficient of 2.58 Å(3) Da(-1) and solvent content of 52.32% suggested the presence of six protein subunits in the asymmetric unit.
Topics: Crystallization; Crystallography, X-Ray; Planococcus Bacteria; beta-Galactosidase
PubMed: 21821893
DOI: 10.1107/S1744309111022627 -
Acta Biochimica Polonica 2014c23o gene, encoding catechol 2,3-dioxygenase from Planococcus sp. strain S5 was randomly mutagenized to generate variant forms of the enzyme with higher degradation...
c23o gene, encoding catechol 2,3-dioxygenase from Planococcus sp. strain S5 was randomly mutagenized to generate variant forms of the enzyme with higher degradation activity. Additionally, the effect of introduced mutations on the enzyme structure was analyzed based on the putative 3D models the wild-type and mutant enzymes. C23OB58 and C23OB81 mutant proteins with amino acid substitutions in close proximity to the enzyme surface or at the interface and in the vicinity of the enzyme active site respectively showed the lowest activity towards all catecholic substrates. The relative activity of C23OC61 mutant towards para-substituted catechols was 20-30% lower of the wild-type enzyme. In this mutant all changes: F191I, C268R, Y272H, V280A and Y293D were located within the conserved regions of C-terminal domain. From these F191I seems to have significant implications for enzyme activity. The highest activity towards different catechols was found for mutant C23OB65. R296Q mutation improved the activity of C23O especially against 4-chlorocatechol. The relative activity of above-mentioned mutant detected against this substrate was almost 6-fold higher than the wild-type enzyme. These results should facilitate future engineering of the enzyme for bioremediation.
Topics: Bacterial Proteins; Catechol 2,3-Dioxygenase; Catechols; Mutagenesis; Planococcus Bacteria; Protein Structure, Secondary; Substrate Specificity
PubMed: 25337606
DOI: No ID Found -
Current Microbiology Oct 2016Several reports have highlighted that many plant growth-promoting endophytic bacteria (PGPE) can assist their host plants in coping with various biotic and abiotic...
Several reports have highlighted that many plant growth-promoting endophytic bacteria (PGPE) can assist their host plants in coping with various biotic and abiotic stresses. However, information about the PGPE colonizing in the halophytes is still scarce. This study was designed to isolate and characterize PGPE from salt-accumulating halophyte Salicornia europaea grown under extreme salinity and to evaluate in vitro the bacterial mechanisms related to plant growth promotion. A total of 105 isolates were obtained from the surface-sterilized roots, stems, and assimilation twigs of S. europaea. Thirty-two isolates were initially selected for their ability to produce 1-aminocyclopropane-1-carboxylate deaminase as well as other properties such as production of indole-3-acetic acid and phosphate-solubilizing activities. The 16S rRNA gene-sequencing analysis revealed that these isolates belong to 13 different genera and 19 bacterial species. For these 32 strains, seed germination and seedling growth in axenically grown S. europaea seedlings at different NaCl concentrations (50-500 mM) were quantified. Five isolates possessing significant stimulation of the host plant growth were obtained. The five isolates were identified as Bacillus endophyticus, Bacillus tequilensis, Planococcus rifietoensis, Variovorax paradoxus, and Arthrobacter agilis. All the five strains could colonize and can be reisolated from the host plant interior tissues. These results demonstrate that habitat-adapted PGPE isolated from halophyte could enhance plant growth under saline stress conditions.
Topics: Bacteria; Chenopodiaceae; Endophytes; Indoleacetic Acids; Plant Growth Regulators; Plant Roots; Salt-Tolerant Plants; Sodium Chloride
PubMed: 27447799
DOI: 10.1007/s00284-016-1096-7 -
The Journal of General and Applied... 2012Bacterial strains capable of degrading trichlorophenol (2,4,6-TCP) were isolated from the secondary sludge of a pulp and paper mill and were characterized. These...
Bacterial strains capable of degrading trichlorophenol (2,4,6-TCP) were isolated from the secondary sludge of a pulp and paper mill and were characterized. These isolates were identified as Planococcus rifietoensis (CL4) and Bacillus pumilus (CL5), based on their 16S rRNA sequence analysis. These isolates were able to grow and utilize 2,4,6-TCP as their source of carbon as well as energy. HPLC analysis and stoichometric release of chloride in the medium confirmed the degradation ability of these isolates. Removal efficiency of 2,4,6-TCP by these isolates was discovered to be high. They were able to remove 90% of 2,4,6-TCP when grown at a concentration of 600 mg L(-1). Inoculation of these bacteria completely removed 2,4,6-TCP within 2 weeks from the sludge of the pulp and paper mill when supplemented at the rate of 100 mg L(-1). Absorbable Organic Halogen (AOX) and Extractable Organic Halogen (EOX) were significantly reduced by 63% and 70% respectively from the sludge due to inoculation of these bacteria. These isolates have high potential to remove 2,4,6-TCP and may be used for removal of 2,4,6-TCP from pulp paper mill waste.
Topics: Bacillus; Bacterial Typing Techniques; Biodegradation, Environmental; Chlorophenols; DNA, Bacterial; Industrial Waste; Molecular Sequence Data; Paper; Phylogeny; Planococcus Bacteria; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Sewage; Waste Disposal, Fluid; Water Pollutants, Chemical
PubMed: 23337576
DOI: 10.2323/jgam.58.413 -
Bioresource Technology Jan 2015The present study investigates the impacts of phase separated disintegration through CaCl2 (calcium chloride) mediated biosurfactant producing bacterial pretreatment. In...
The present study investigates the impacts of phase separated disintegration through CaCl2 (calcium chloride) mediated biosurfactant producing bacterial pretreatment. In the initial phase of the study, the flocs were disintegrated (deflocculation) with 0.06g/gSS of CaCl2. In the subsequent phase, the sludge biomass was disintegrated (cell disintegration) through potent biosurfactant producing new novel bacteria, Planococcus jake 01. The pretreatment showed that suspended solids reduction and chemical oxygen demand solubilization for deflocculated - bacterially pretreated sludge was found to be 17.14% and 14.14% which were comparatively higher than flocculated sludge (treated with bacteria alone). The biogas yield potential of deflocculated - bacterially pretreated, flocculated, and control sludges were observed to be 0.322(L/gVS), 0.225(L/gVS) and 0.145(L/gVS) respectively. To our knowledge, this is the first study to present the thorough knowledge of biogas production potential through a novel phase separated biosurfactant bacterial pretreatment.
Topics: Anaerobiosis; Biodegradation, Environmental; Biofuels; Biological Oxygen Demand Analysis; Biomass; Biotechnology; Calcium Chloride; Flocculation; Planococcus Bacteria; Sewage; Surface-Active Agents; Waste Disposal, Fluid
PubMed: 25459848
DOI: 10.1016/j.biortech.2014.10.122 -
Microbial Ecology Oct 2016Heavy metals influence the population size, diversity, and metabolic activity of bacteria. In turn, bacteria can develop heavy metal resistance mechanisms, and this can...
Diversity and Distribution of Heavy Metal-Resistant Bacteria in Polluted Sediments of the Araça Bay, São Sebastião (SP), and the Relationship Between Heavy Metals and Organic Matter Concentrations.
Heavy metals influence the population size, diversity, and metabolic activity of bacteria. In turn, bacteria can develop heavy metal resistance mechanisms, and this can be used in bioremediation of contaminated areas. The purpose of the present study was to understand how heavy metals concentration influence on diversity and distribution of heavy metal-resistant bacteria in Araça Bay, São Sebastião, on the São Paulo coast of Brazil. The hypothesis is that activities that contribute for heavy metal disposal and the increase of metals concentrations in environment can influence in density, diversity, and distribution of heavy metal-resistant bacteria. Only 12 % of the isolated bacteria were sensitive to all of the metals tested. We observed that the highest percentage of resistant strains were in areas closest to the São Sebastião channel, where port activity occurs and have bigger heavy metals concentrations. Bacterial isolated were most resistant to Cr, followed by Zn, Cd, and Cu. Few strains resisted to Cd levels greater than 200 mg L(-1). In respect to Cr, 36 % of the strains were able to grow in the presence of as much as 3200 mg L(-1). Few strains were able to grow at concentrations of Zn and Cu as high as 1600 mg L(-1), and none grew at the highest concentration of 3200 mg L(-1). Bacillus sp. was most frequently isolated and may be the dominant genus in heavy metal-polluted areas. Staphylococcus sp., Planococcus maritimus, and Vibrio aginolyticus were also isolated, suggesting their potential in bioremediation of contaminated sites.
Topics: Bacillus; Bacteria; Base Sequence; Bays; Biodegradation, Environmental; Biodiversity; Brazil; Cadmium; Chromium; Copper; DNA, Bacterial; Drug Resistance, Multiple, Bacterial; Environmental Monitoring; Geologic Sediments; Metals, Heavy; Microbial Sensitivity Tests; Microbiological Techniques; Multivariate Analysis; Organic Chemicals; RNA, Ribosomal, 16S; Water Pollutants, Chemical; Zinc
PubMed: 27480227
DOI: 10.1007/s00248-016-0821-x -
Standards in Genomic Sciences 2018Y42, isolated from the petroleum-contaminated soil of the Qaidam Basin, can use crude oil as its sole source of carbon and energy at 20 °C. The genome of strain Y42...
Y42, isolated from the petroleum-contaminated soil of the Qaidam Basin, can use crude oil as its sole source of carbon and energy at 20 °C. The genome of strain Y42 has been sequenced to provide information on its properties. Genomic analysis shows that the genome of strain Y42 contains one circular DNA chromosome with a size of 3,718,896 bp and a GC content of 48.8%, and three plasmids (329,482; 89,073; and 12,282 bp). Although the strain Y42 did not show a remarkably higher ability in degrading crude oil than other oil-degrading bacteria, the existence of strain Y42 played a significant role to reducing the overall environmental impact as an indigenous oil-degrading bacterium. In addition, genome annotation revealed that strain Y42 has many genes responsible for hydrocarbon degradation. Structural features of the genomes might provide a competitive edge for strain Y42 to survive in oil-polluted environments and be worthy of further study in oil degradation for the recovery of crude oil-polluted environments.
PubMed: 30338026
DOI: 10.1186/s40793-018-0328-9 -
Extremophiles : Life Under Extreme... May 2018A psychrophilic extracellular protease was isolated from the marine bacterium Planococcus sp. M7 found in the deep-sea mud of the Southern Indian Ocean. The mature...
A psychrophilic extracellular protease was isolated from the marine bacterium Planococcus sp. M7 found in the deep-sea mud of the Southern Indian Ocean. The mature protease is about 43 kDa and contains 389 amino acids. Sequence alignment revealed that the protease whose catalytic triad was comprised of Ser224, Lys249, and Gln253 contains a catalytic module belonging to the serralysin-type protease family 41, and displays 46.55% identity with the experimentally verified serine protease from Bacillus subtilis str. 168. The enzyme displayed an alkaline mesophilic preference with an optimum pH of 10.0 and an optimum temperature of 35 °C. The enzyme retained its activity from 5 to 35 °C and was resistant to repeated freezing and thawing, but was completely inactivated at 55 °C. Calcium ions had a protective effect against thermal denaturation. More than 60% of the maximum activity was retained at pH values in the range of 5.0-11.0. Almost no activity loss was detected after 1 h of incubation at pH 8.0-10.0 and 20 °C, or with 1.0% SDS. Most important, this protease also showed good stability and compatibility with the standard enzyme-free detergent, which indicates its special interest for applications in detergent industry.
Topics: Bacterial Proteins; Enzyme Stability; Freezing; Peptide Hydrolases; Planococcus Bacteria; Protein Denaturation
PubMed: 29497843
DOI: 10.1007/s00792-018-1010-2 -
International Journal of Systematic and... May 2022Three strains of members of the family , which can inhibit the growth of some Gram-stain-positive strains, designated M4U3P1, HD4P25 and RD4P76, were isolated from...
Three strains of members of the family , which can inhibit the growth of some Gram-stain-positive strains, designated M4U3P1, HD4P25 and RD4P76, were isolated from halophytes in Baotou, Inner Mongolia, PR China. A phylogenetic analysis based on the 16S rRNA gene and the whole genome sequences revealed that HD4P25 clustered with YIM 93174 with a similarity of 98.4 %, and RD4P76 shared the highest similarity of 16S rRNA gene with SA4 (97.5 %). M4U3P1 clustered with strains of genera and based on whole-genome sequence analyses, but its 16S rRNA gene had the highest similarity to '' EGI 80668 (96.1 %). The average nucleotide's identity by blast (ANIb) and digital DNA-DNA hybridization (dDDH) values of the three isolated strains to their close relatives were well below the threshold value for identifying a novel species.On the basis of the phylogenetic, physiological and phenotypic results, gen. nov., sp. nov. [type strain M4U3P1 (=CGMCC 1.17076=JCM 33851)] sp. nov. [type strain HD4P25 (=CGMCC 1.18651 =JCM 34524)] and sp. nov. [type strain RD4P76 (=CGMCC 1.18659=JCM 34525)] were proposed, respectively. All three species are ubiquitous in the bulk saline-alkaline soils, but only the species represented by strain RD4P76 was widely distributed in the rhizosphere soil, the above-ground part and the roots of . The species represented by M4U3P1 can be detected in the roots of , and rarely detected in the above-ground parts of . The species represented by HD4P25 was rarely detected in the interior of . The three strains could inhibit some of the Gram-stain-positive bacteria (i.e. members of the genera , and ) in the saline-alkali soil. A genomic analysis of these three strains revealed that they can synthesize different antagonistic compounds, such as aminobenzoate and bacitracin or subtilisin.
Topics: Bacillaceae; Bacillus; Bacterial Typing Techniques; Base Composition; Chenopodiaceae; DNA, Bacterial; Fatty Acids; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Salt-Tolerant Plants; Sequence Analysis, DNA; Soil
PubMed: 35550242
DOI: 10.1099/ijsem.0.005337