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Fetal Diagnosis and Therapy 2008Does formation of platelet-derived microparticles correspond to platelet activation? (Comparative Study)
Comparative Study
OBJECTIVE
Does formation of platelet-derived microparticles correspond to platelet activation?
METHODS
The study was performed in 51 preterm newborns, 25 girls and 26 boys. The control group consisted of 55 term newborns, 25 girls and 30 boys. Blood samples were collected from the umbilical artery. The percentage of platelet-derived microparticles and platelet count were determined using flow cytometric analysis based on the CD61-positive antigen.
RESULTS
The percentage of platelet-derived microparticles was higher in preterm newborns (5.46) in comparison to term newborns (4.22, p < 0.01). We found 4.61% of platelet-derived microparticles in preterm female newborns and 6.28% in preterm boys (p < 0.0070). The platelet count was 256 x 10(3) microl in girls and 238 x 10(3) microl in boys. Female healthy term newborns presented higher values of platelet-derived microparticles (4.4%) than male newborns (4.07%, p = 0.4725, table 1). The platelet count in girls was found to be 308 x 10(3) microl and in boys 270 x 10(3) microl.
CONCLUSIONS
Higher percentage of platelet-derived microparticles in preterm newborns may provide a compensatory mechanism for the hemostatic system.
Topics: Cell Separation; Female; Flow Cytometry; Humans; Infant, Newborn; Infant, Premature; Male; Platelet Activation; Platelet Count
PubMed: 18046075
DOI: 10.1159/000111597 -
Seminars in Cardiothoracic and Vascular... Dec 2020. We hypothesize that preoperative functional platelet number (platelet count multiplied by platelet aggregation percentage) are associated with 30-day mortality after...
. We hypothesize that preoperative functional platelet number (platelet count multiplied by platelet aggregation percentage) are associated with 30-day mortality after cardiac surgery. . We linked our preoperative testing database with the STS (Society of Thoracic Surgeon) database to form a study cohort of 1390 patients who had cardiac surgeries between January 2008 and December 2013. Preoperative tests of platelet count and platelet aggregation were routinely performed on all cardiac surgical patients within 24 hours before entering the operating room. Multiple logistic regression models were used to determine whether functional platelet number are associated with 30-day mortality, modified composite major adverse cardiocerebral events, postoperative renal failure or requirement for new renal replacement therapy, and reoperation for bleeding. Log-linear models were used to examine whether functional platelet numbers are associated with hospital length of stay and intensive care unit length of stay. . Functional platelet number had an inverse association with 30-day mortality, and each 50 × 10/L increase in functional platelet number resulted in decreased 30-day mortality (odds ratio of 0.767 with 95% confidence interval = 0.591-0.996). For secondary outcomes, functional platelet number was neither associated with major adverse cardiocerebral event nor length of stay. However, we found that each 50 × 10/L increase in functional platelet number was associated with decreased reoperations for bleeding (odds ratio of 0.778 with 95% confidence interval = 0.636-0.951). . The preoperative functional platelet number had significant associations with 30-day mortality after cardiac surgery. Functional platelet number could be used to guide timing of cardiac surgery, especially as more and more patients are receiving antiplatelet medications nowadays.
Topics: Aged; Cardiac Surgical Procedures; Cohort Studies; Databases, Factual; Female; Humans; Male; Middle Aged; Platelet Aggregation; Platelet Count; Postoperative Complications; Preoperative Period; Retrospective Studies; Time
PubMed: 32698733
DOI: 10.1177/1089253220943023 -
European Journal of Haematology Jul 1995
Topics: Blood Platelets; Edetic Acid; Humans; Platelet Count; Thrombocytopenia
PubMed: 7615055
DOI: 10.1111/j.1600-0609.1995.tb00240.x -
Clinics in Laboratory Medicine Mar 2015Many modern automated cell counters in high-volume clinical hematology laboratories use new, improved technologies for routine platelet analysis. The latest progress... (Review)
Review
Many modern automated cell counters in high-volume clinical hematology laboratories use new, improved technologies for routine platelet analysis. The latest progress includes the use of state-of-the art information technology, specific fluorescent dyes, and monoclonal antibodies to obtain more reliable platelet counts. This information allows the accurate and precise enumeration of platelets even in thrombocytopenic patients and the reporting of novel platelet parameters. In the near future, digital image analysis may permit even better platelet analysis.
Topics: Automation; History, 20th Century; History, 21st Century; Platelet Count
PubMed: 25676376
DOI: 10.1016/j.cll.2014.11.002 -
Archives of Pathology & Laboratory... Jul 2003
Topics: Heparin; Humans; Monitoring, Physiologic; Platelet Count; Thrombocytopenia
PubMed: 12823032
DOI: 10.5858/2003-127-782c-PCMALT -
International Journal of Laboratory... Jun 2021Accurate platelet counting is essential for risk assessment of bleeding and thrombosis. Abbott Alinity hq hematology analyzer was recently introduced, and its... (Comparative Study)
Comparative Study
INTRODUCTION
Accurate platelet counting is essential for risk assessment of bleeding and thrombosis. Abbott Alinity hq hematology analyzer was recently introduced, and its performance in platelet counting has yet to be evaluated comprehensively. In this study, we evaluated the performance of the optical platelet counting of Abbott Alinity hq (Alinity-PLT) and the impedance and fluorescent platelet counting of Sysmex XN-9000 (XN-PLT-I and XN-PLT-F) compared with the international reference method.
METHODS
Blood samples were analyzed via Alinity hq and XN-9000 with PLT-F channel. Immuno-platelet (ImmnoPLT) reference method was performed with CD41/CD61 antibodies using FACSLyric flow cytometer (BD). Precision was determined using 10 replicates in a single run, and the platelet counts of Alinity-PLT, XN-PLT-I, XN-PLT-F, and ImmnoPLT were compared.
RESULTS
At a platelet count of 13 × 10 /L, the CVs of Alinity-PLT, XN-PLT-I, and XN-PLT-F were 4.2%, 6.7%, and 4.3%, respectively, and at a platelet count of 44 × 10 /L, all showed a CV of less than 3%. For the total 210 samples, all three methods showed a very strong correlation with ImmunoPLT (r > 0.99). For platelet levels below 20 × 10 /L, XN-PLT-F showed the strongest correlation with ImmunoPLT (r = 0.975), and for platelet levels of 20-100 × 10 /L, Alinity-PLT and XN-PLT-I were comparable to ImmunoPLT. For platelet levels of 100-450 × 10 /L, XN-PLT-I was the most comparable to ImmunoPLT, and for platelet levels above 450 × 10 /L, Alinity-PLT was comparable to ImmunoPLT.
CONCLUSIONS
All three methods were highly correlated with ImmunoPLT, and each method had different performance advantages according to the platelet levels.
Topics: Blood Platelets; Flow Cytometry; Hemorrhage; Humans; Platelet Count; Reproducibility of Results; Thrombosis
PubMed: 33217153
DOI: 10.1111/ijlh.13396 -
Journal of Veterinary Internal Medicine Jul 2021Vincristine might increase circulating platelet numbers but the functional capacity of these newly released platelets is unknown.
BACKGROUND
Vincristine might increase circulating platelet numbers but the functional capacity of these newly released platelets is unknown.
OBJECTIVE
To evaluate and compare the functionality of mature and immature (reticulated) platelets after a single intravenous dose of vincristine in dogs.
ANIMALS
Ten healthy purpose-bred dogs.
METHODS
Dogs prospectively received a single IV injection of 0.02 mg/kg vincristine or 0.9% saline. Before and after treatment on days 3, 5, and 7, platelets (resting and after thrombin stimulation) were assessed by flow cytometric determination of P-selectin (CD62P) expression. Reticulated platelets were distinguished using thiazole orange (TO) staining.
RESULTS
Relative to saline, vincristine administration increased platelet count from day 0 to day 7 (225 ± 58 to 273 ± 65 × 10 /μL, vs 299 ± 76.4 to 214 ± 20 × 10 /μL, P = .01) and increased percentage of reticulated platelets from day 0 to day 5 (3.9 ± 1.5% to 6.1 ± 1.6%, P = .02). On all days, reticulated platelets had greater resting expression of CD62P than did mature platelets (49.6 ± 4% vs 10.2 ± 1%, P ≤ .001). Across all days, CD62P expression by reticulated platelets in the vincristine and saline-treated groups was not different when unstimulated (P = .7) or after thrombin stimulation (P = .33).
CONCLUSIONS AND CLINICAL IMPORTANCE
Reticulated platelets released in response to vincristine administration function similarly to mature platelets.
Topics: Animals; Blood Platelets; Dogs; Flow Cytometry; Platelet Count; Vincristine
PubMed: 33993531
DOI: 10.1111/jvim.16169 -
Archives of Pathology & Laboratory... Jan 2024Platelet (PLT) counting with impedance (PLT-I) is widely used but has low specificity. PLT counting with fluorescence (PLT-F), tested by the Sysmex XN series with high...
Micro-Red Blood Cell, Fragmented Red Blood Cell, Platelet Distribution Width, Mean Platelet Volume, and Platelet-Large Cell Ratio on Sysmex XN Series Hematology Analyzers Can Be Used for the Reflex Test of Impedance Platelet Count in Clinical Practice.
CONTEXT.—
Platelet (PLT) counting with impedance (PLT-I) is widely used but has low specificity. PLT counting with fluorescence (PLT-F), tested by the Sysmex XN series with high specificity, can be a complementary method to PLT-I.
OBJECTIVE.—
To identify red blood cell (RBC)- and PLT-related parameters as potential influencing factors for PLT-I and establish PLT reflex test rules with PLT-F.
DESIGN.—
We prospectively tested both PLT-I and PLT-F in all 3480 samples. In a development data set of 3000 samples, differences between the reflex and nonreflex groups were compared and influencing factors for PLT-I were identified by logistic regression. The area under the receiver operating characteristic (ROC) curve and cutoff values were obtained by ROC curve analysis. Validation was conducted in the remaining 480 samples (validation data set).
RESULTS.—
PLT-F showed comparable results with immunoplatelet counting. In logistic regression, increased micro-RBC absolute count (micro-RBC#), fragmented RBC absolute count (FRC#), PLT distribution width (PDW), mean PLT volume (MPV), PLT-large cell ratio (P-LCR), and immature PLT fraction absolute count (IPF#) were influencing factors for PLT-I. In ROC curve analysis, the cutoff values of micro-RBC#, FRC#, PDW, MPV, and P-LCR were 0.64 × 106/μL, 0.082 × 106/μL, 15.40 fL, 11.15 fL, and 33.95%, respectively. The areas under the ROC curve of micro-RBC# and FRC# were 0.77 and 0.79, respectively.
CONCLUSIONS.—
Micro-RBC#, FRC#, PDW, MPV, P-LCR, and IPF# were factors affecting PLT-I. Among them, micro-RBC# and FRC# were the most impactful factors. From our study results, micro-RBC#, FRC#, MPV, PDW, and P-LCR can be used to establish reflex test rules for PLT counting in clinical work.
Topics: Humans; Platelet Count; Mean Platelet Volume; Electric Impedance; Blood Platelets; Erythrocytes; Hematology
PubMed: 37014982
DOI: 10.5858/arpa.2022-0030-OA -
Transfusion and Apheresis Science :... Dec 2012Refractoriness to platelet transfusions is a common clinical problem. The present study was conducted to look into patient characteristics affecting the corrected count...
Refractoriness to platelet transfusions is a common clinical problem. The present study was conducted to look into patient characteristics affecting the corrected count increment in a tertiary care referral hospital. A total of 161 aphaeresis platelet units were transfused to 40 patients with varied clinical diagnoses. The mean platelet increment was 17,789/mm(3). Median corrected count increment was 7344 and percentage platelet recovery was 22.82%. Logistic regression analysis revealed significant influence of splenomegaly and anti-platelet drugs on refractoriness. Fever, bleeding, sepsis, disseminated intravascular coagulation and cyclosporine use, though more common in the patients with refractoriness they were not statistically significant.
Topics: Adolescent; Adult; Aged; Child; Child, Preschool; Female; Humans; Male; Middle Aged; Platelet Count; Platelet Transfusion; Plateletpheresis; Prospective Studies; Young Adult
PubMed: 22705296
DOI: 10.1016/j.transci.2012.04.006 -
Clinical and Laboratory Haematology Apr 2004
Topics: Electric Impedance; Humans; Optics and Photonics; Platelet Count
PubMed: 15053812
DOI: 10.1111/j.1365-2257.2004.00596.x