-
PloS One 2021Polioviruses are positive-sense, single-stranded RNA picornaviruses and the principal cause of poliomyelitis. Global poliovirus surveillance has relied on poliovirus...
Polioviruses are positive-sense, single-stranded RNA picornaviruses and the principal cause of poliomyelitis. Global poliovirus surveillance has relied on poliovirus isolation in cells, which may take a minimum of 10 days, involves maintaining two cell lines, and propagates virus in high titers. With eradication underway, a major objective of the Global Polio Eradication Initiative (GPEI) is to develop culture-independent detection of polioviruses as an alternative method to complement the current virus isolation technique. A culture-independent method on poliovirus-positive stool suspensions was assessed with commercially available recombinant soluble poliovirus receptor (PVR) coupled to Histidine (His) tags. Viral RNA was screened by quantitative real-time reverse transcription PCR using the poliovirus intratypic differentiation kit. Poliovirus recovery was optimized with PVR-His-tagged protein and buffers supplemented with polyethylene glycol. To validate the poliovirus-PVR-His tag purification assay, 182 poliovirus-positive stools of programmatic importance were parallel tested against the GPLN-accepted virus isolation method. The PVR-His tag enrichment method detected poliovirus in 164 of 171 poliovirus-positive stools, whereas the virus isolation method misidentified 38 stools as poliovirus-negative (McNemar χ2 p<0.0001). Using this method in combination with RNA extraction, viral RNA recovery increased and showed similar (WPV1) or higher (Sabin 1) sensitivity than the World Health Organization accredited variation of the virus isolation method. The PVR-His enrichment method could be a viable addition to poliovirus surveillance; similar methods have the potential to capture other human pathogens such as EV71 using an appropriate soluble His tag receptor.
Topics: Feces; Poliomyelitis; Poliovirus
PubMed: 34727100
DOI: 10.1371/journal.pone.0259099 -
Food and Environmental Virology Dec 2017Poliovirus surveillance plays a critical role in achieving and certifying eradication and will play a key role in the polio endgame. Environmental surveillance can... (Review)
Review
Poliovirus surveillance plays a critical role in achieving and certifying eradication and will play a key role in the polio endgame. Environmental surveillance can provide an opportunity to detect circulating polioviruses prior to the observation of any acute flaccid paralysis cases. We completed a systematic review of peer-reviewed publications on environmental surveillance for polio including the search terms "environmental surveillance" or "sewage," and "polio," "poliovirus," or "poliomyelitis," and compared characteristics of the resulting studies. The review included 146 studies representing 101 environmental surveillance activities from 48 countries published between 1975 and 2016. Studies reported taking samples from sewage treatment facilities, surface waters, and various other environmental sources, although they generally did not present sufficient details to thoroughly evaluate the sewage systems and catchment areas. When reported, catchment areas varied from 50 to over 7.3 million people (median of 500,000 for the 25% of activities that reported catchment areas, notably with 60% of the studies not reporting this information and 16% reporting insufficient information to estimate the catchment area population size). While numerous studies reported the ability of environmental surveillance to detect polioviruses in the absence of clinical cases, the review revealed very limited information about the costs and limited information to support quantitative population effectiveness of conducting environmental surveillance. This review motivates future studies to better characterize poliovirus environmental surveillance systems and the potential value of information that they may provide in the polio endgame.
Topics: Environmental Monitoring; Fresh Water; Humans; Poliomyelitis; Poliovirus; Sewage
PubMed: 28687986
DOI: 10.1007/s12560-017-9314-4 -
Food and Environmental Virology Dec 2018Within the initiatives for poliomyelitis eradication by WHO, Italy activated an environmental surveillance (ES) in 2005. ES complements clinical Acute Flaccid Paralysis...
Within the initiatives for poliomyelitis eradication by WHO, Italy activated an environmental surveillance (ES) in 2005. ES complements clinical Acute Flaccid Paralysis (AFP) surveillance for possible polio cases, detects poliovirus circulation in environmental sewage, and is used to monitor transmission in communities. In addition to polioviruses, the analyses comprised: (i) the monitoring of the presence of non-polio enteroviruses in sewage samples and (ii) the temporal and geographical distribution of the detected viruses. From 2009 to 2015, 2880 sewage samples were collected from eight cities participating in the surveillance. Overall, 1479 samples resulted positive for enteroviruses. No wild-type polioviruses were found, although four Sabin-like polioviruses were detected. The low degree of mutation found in the genomes of these four isolates suggests that these viruses have had a limited circulation in the population. All non-polio enteroviruses belonged to species B and the most frequent serotype was CV-B5, followed by CV-B4, E-11, E-6, E-7, CV-B3, and CV-B2. Variations in the frequency of different serotypes were also observed in different seasons and/or Italian areas. Environmental surveillance in Italy, as part of the 'WHO global polio eradication program', is a powerful tool to augment the polio surveillance and to investigate the silent circulation or the re-emergence of enteroviruses in the population.
Topics: Cities; Enterovirus; Enterovirus Infections; Environmental Monitoring; Humans; Italy; Limit of Detection; Poliomyelitis; Poliovirus; Sewage
PubMed: 29948963
DOI: 10.1007/s12560-018-9350-8 -
Japanese Journal of Infectious Diseases Nov 2018By monitoring the sewage system in Heilongjiang province from 2013 to 2016, this study aimed to analyze the epidemiological tendency and genetic mutation of poliovirus...
By monitoring the sewage system in Heilongjiang province from 2013 to 2016, this study aimed to analyze the epidemiological tendency and genetic mutation of poliovirus (PV) found in the environment in order to setup a warning system for vaccine-derived poliovirus (VDPV) and the spread of wild poliovirus. In this study, we collected 139 sewage samples from 8 regions in Heilongjiang province. Poliovirus was identified from 72 samples, and the positivity rate was 51%. A total of 263 PV strains were isolated, including 22 strains of type 1 PV, 104 strains of type 2 PV, and 137 strains of type 3 PV. As a result of intratypic differentiation, using real-time PCR and nucleotide sequencing, 3 type 1 pre-VDPV, one type 2 VDPV, and 2 type 3 pre-VDPV strains were isolated. Interestingly, one type 1 strain with 5 nucleotide deletions and one type 3 recombinant on VP1 were isolated. By continuously monitoring the poliovirus in the environment, we aimed to recognize the VDPV or wild poliovirus with high neurovirulence from large-scale circulation and set up a warning system to avoid morbidity and virus transmission.
Topics: China; Genetic Variation; Genotype; Genotyping Techniques; Humans; Poliovirus; Real-Time Polymerase Chain Reaction; Sequence Analysis, DNA; Sewage
PubMed: 30068885
DOI: 10.7883/yoken.JJID.2017.338 -
Journal of Bacteriology Sep 1963Wallis, Craig (Baylor University College of Medicine, Houston, Tex.) and Joseph L. Melnick. Thermosensitivity of poliovirus. J. Bacteriol. 86:499-504. 1963.-Polioviruses...
Wallis, Craig (Baylor University College of Medicine, Houston, Tex.) and Joseph L. Melnick. Thermosensitivity of poliovirus. J. Bacteriol. 86:499-504. 1963.-Polioviruses are thermosensitive agents, although thermoresistant strains have been obtained and reported in the literature. Such resistant strains can be developed by exposure of the virus to cystine during multiple-cycle yields. Thermoresistant strains can be converted to the thermosensitive state by passing the virus in cells maintained in a cystine-free medium, or by reducing the virus with glutathione. The thermoresistant variants seem to result from the conditions under which virus is grown and harvested. Consequently, many such thermostable polioviruses actually represent phenotypic rather than genotypic variation.
Topics: Hot Temperature; Poliovirus; Research
PubMed: 14066428
DOI: 10.1128/jb.86.3.499-504.1963 -
Virus Research Feb 2005Positive strand RNA virus populations are a collection of similar but genetically different viruses. They exist as viral quasispecies due to the high mutation rates of... (Review)
Review
Positive strand RNA virus populations are a collection of similar but genetically different viruses. They exist as viral quasispecies due to the high mutation rates of the low fidelity viral RNA-dependent RNA polymerase (RdRp). It is thought that this genomic heterogeneity is advantageous to the population, allowing for adaptation to rapidly changing environments that present varying types and degrees of selective pressure. However, one consequence of this extensive diversity is the susceptibility to mutagens that further increase sequence variation. Since RNA viruses live at the edge of maximal variability, an increase in the mutation rate is likely to force the virus beyond the tolerable mutation frequency into 'error catastrophe'. One such mutagen, ribavirin, is an antiviral nucleoside analog that is mutagenic to several RNA viruses. Ribavirin is incorporated into the viral genome causing lethal mutagenesis and a subsequent decrease in the specific infectivity. Even so, passaging poliovirus in the presence of low to intermediate concentrations of the drug leads to the emergence of a viral population resistant to the effects of ribavirin. These viruses have a point mutation in the RdRp that increases the overall polymerase fidelity. Interestingly, as predicted by the quasispecies theory, ribavirin resistant viruses are less adaptable, as they are more susceptible to other non-mutagenic antiviral drugs and are highly attenuated in vivo. Here, we review the mechanism of action of ribavirin on poliovirus and other RNA viruses, the possibility for escape via increased fidelity of the viral polymerase, the consequences of this response on viral population dynamics, and the biological implications for the therapeutic use of mutagenic antiviral agents.
Topics: Antiviral Agents; Drug Resistance, Viral; Mutation; Poliovirus; RNA-Dependent RNA Polymerase; Ribavirin
PubMed: 15649563
DOI: 10.1016/j.virusres.2004.11.007 -
The Journal of Infectious Diseases Nov 2014The attenuated oral poliovirus vaccine (OPV) has many properties favoring its use in polio eradication: ease of administration, efficient induction of intestinal... (Review)
Review
The attenuated oral poliovirus vaccine (OPV) has many properties favoring its use in polio eradication: ease of administration, efficient induction of intestinal immunity, induction of durable humoral immunity, and low cost. Despite these advantages, OPV has the disadvantage of genetic instability, resulting in rare and sporadic cases of vaccine-associated paralytic poliomyelitis (VAPP) and the emergence of genetically divergent vaccine-derived polioviruses (VDPVs). Whereas VAPP is an adverse event following exposure to OPV, VDPVs are polioviruses whose genetic properties indicate prolonged replication or transmission. Three categories of VDPVs are recognized: (1) circulating VDPVs (cVDPVs) from outbreaks in settings of low OPV coverage, (2) immunodeficiency-associated VDPVs (iVDPVs) from individuals with primary immunodeficiencies, and (3) ambiguous VDPVs (aVDPVs), which cannot be definitively assigned to either of the first 2 categories. Because most VDPVs are type 2, the World Health Organization's plans call for coordinated worldwide replacement of trivalent OPV with bivalent OPV containing poliovirus types 1 and 3.
Topics: Genotype; Humans; Poliovirus; Poliovirus Vaccine, Oral; Vaccines, Attenuated; Virulence; Virus Shedding
PubMed: 25316847
DOI: 10.1093/infdis/jiu295 -
Biologicals : Journal of the... Jun 2006Seven cases of long-term poliovirus excretion in the UK and Ireland are reviewed in this paper. They include a rare case of long-term virus excretion by a healthy child... (Review)
Review
Seven cases of long-term poliovirus excretion in the UK and Ireland are reviewed in this paper. They include a rare case of long-term virus excretion by a healthy child recently found in Ireland and the case with the longest period of vaccine-derived poliovirus excretion by an immunodeficient individual ever known, 18 years. The evolution of viral properties such as antigenic structure, neurovirulence, sensitivity for growth at high temperatures, and differences in nucleotide sequence from the Sabin vaccine strains were studied in detail. The relevance of these cases in the context of the global polio eradication initiative and the design of vaccination strategies for the last stages of eradication and the post-eradication era are discussed.
Topics: Child; Humans; Poliomyelitis; Poliovirus; Population Surveillance; Virus Shedding
PubMed: 16650772
DOI: 10.1016/j.biologicals.2006.02.005 -
The Journal of Clinical Investigation Jun 2004Poliovirus replication is limited to a few organs, including the brain and spinal cord. This restricted tropism may be a consequence of organ-specific differences in...
Poliovirus replication is limited to a few organs, including the brain and spinal cord. This restricted tropism may be a consequence of organ-specific differences in translation initiation by the poliovirus internal ribosome entry site (IRES). A C-to-U mutation at base 472 in the IRES of the Sabin type 3 poliovirus vaccine strain, known to attenuate neurovirulence, may further restrict tropism by eliminating viral replication in the CNS. To determine the relationship between IRES-mediated translation and poliovirus tropism, recombinant human adenoviruses were used to express bicistronic mRNAs in murine organs. The IRESs of poliovirus, the cardiotropic coxsackievirus B3 (CVB3), and the hepatotropic hepatitis C virus (HCV) mediate translation in many organs, including those that do not support viral replication. A translation defect associated with the Sabin type 3 IRES was observed in all organs examined. Poliovirus type 1 and recombinant polioviruses dependent on the IRES of CVB3 or HCV replicate in the CNS of mice and cause paralysis. Although the type 3 Sabin strain is an effective vaccine, polioviruses with a U at base 472 of the IRES cause paralysis in newborn mice. Tropism of wild-type and vaccine strains of poliovirus is therefore determined after internal ribosome entry.
Topics: 5' Untranslated Regions; Adenoviridae; Animals; Cell Line; Enterovirus B, Human; Hepacivirus; Humans; Mice; Mice, Inbred C57BL; Nucleic Acid Conformation; Poliomyelitis; Poliovirus; Poliovirus Vaccine, Oral; Protein Biosynthesis; RNA, Viral; Regulatory Sequences, Nucleic Acid; Ribosomes; Survival Rate; Virus Replication
PubMed: 15199409
DOI: 10.1172/JCI21323 -
Bulletin of the World Health... 1980Reliable laboratory techniques for the intratypic characterization of poliovirus types 1, 2, and 3 isolates have an important role in the epidemiological surveillance of...
Reliable laboratory techniques for the intratypic characterization of poliovirus types 1, 2, and 3 isolates have an important role in the epidemiological surveillance of poliomyelitis and in studies of the safety and efficacy of poliovirus vaccines. Of the techniques available for poliovirus strain characterization, those potentially most useful are intratypic serodifferentiation and the biochemical techniques. The value of strain-specific (absorbed) antisera for antigenic characterization of strains has been clearly established for the identification of both vaccine-like viruses and different epidemic wild strains. Single-radial-diffusion techniques appear to be promising and should be further explored. Biochemical techniques involving studies of both virus polypeptides and nucleic acids are also capable of providing valuable information for strain characterization. Biological and physico-chemical tests are generally of limited value but their application may be useful in certain circumstances.
Topics: Epitopes; Poliovirus; World Health Organization
PubMed: 6170471
DOI: No ID Found