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Long-chain polynucleotide filler for skin rejuvenation: efficacy and complications in five patients.Dermatologic Therapy 2016Aging well has become the new target of preventative medicine, and aesthetic dermatology can contribute to this request. The polynucleotide (PN) containing products not...
Aging well has become the new target of preventative medicine, and aesthetic dermatology can contribute to this request. The polynucleotide (PN) containing products not only fill the space, but improve tissue regeneration, resulting in more natural tissue regeneration. Five Korean women received four times injections of long-chain PN filler in two-week intervals for skin rejuvenation. About 0.05 mL of material was injected in 40 points of one-side cheek. The pore and skin thickness were markedly improved in the patients in their 30s, whereas skin tone, melanin, wrinkles, and sagging were noticeably improved for patients in their 40s. There are no serious side effects. In conclusion, intradermal long-chain PN filler injection seems to be an effective and safe treatment for skin rejuvenation.
Topics: Adult; Age Factors; Cosmetic Techniques; Dermal Fillers; Esthetics; Female; Humans; Injections, Intradermal; Middle Aged; Patient Satisfaction; Polynucleotides; Rejuvenation; Skin; Skin Aging; Time Factors; Treatment Outcome
PubMed: 26814448
DOI: 10.1111/dth.12299 -
Federation Proceedings 1965
Review
Topics: Chemical Phenomena; Chemistry; Genetic Code; In Vitro Techniques; Nucleotidyltransferases; Polynucleotides
PubMed: 5322508
DOI: No ID Found -
Journal of Biological Regulators and... 2021
Topics: Collagen; Fibroblasts; Polynucleotides; Skin; Wound Healing
PubMed: 33480222
DOI: 10.23812/20-320-L -
International Journal of Nanomedicine 2007Nanotechnology has tremendously influenced gene therapy research in recent years. Nanometer-size systems have been extensively investigated for delivering genes at both... (Review)
Review
Nanotechnology has tremendously influenced gene therapy research in recent years. Nanometer-size systems have been extensively investigated for delivering genes at both local and systemic levels. These systems offer several advantages in terms of tissue penetrability, cellular uptake, systemic circulation, and cell targeting as compared to larger systems. They can protect the polynucleotide from a variety of degradative and destabilizing factors and enhance delivery efficiency to the cells. A variety of polymeric and non-polymeric nanoparticles have been investigated in an effort to maximize the delivery efficiency while minimizing the toxic effects. This article provides a review on the most commonly used nanoparticulate systems for gene delivery. We have discussed frequently used polymers, such as, polyethyleneimine, poly (lactide-co-glycolide), chitosan, as well as non-polymeric materials such as cationic lipids and metallic nanoparticles. The advantages and limitations of each system have been elaborated.
Topics: Chemistry, Pharmaceutical; Drug Carriers; Gene Targeting; Genetic Therapy; Nanomedicine; Nanostructures; Polynucleotides; Transfection
PubMed: 18019834
DOI: No ID Found -
Artificial Intelligence in Medicine Sep 2007A theory of fuzzy polynucleotides, including an n-dimensional metric fuzzy polynucleotide space, has been previously introduced by the present author for... (Review)
Review
OBJECTIVE
A theory of fuzzy polynucleotides, including an n-dimensional metric fuzzy polynucleotide space, has been previously introduced by the present author for fuzzy-theoretical analysis of nucleic acids [Sadegh-Zadeh K. Fuzzy genomes. Artif Intell Med 2000;18:1-28; Sadegh-Zadeh K. Ein Verfahren zur Fuzzydecodierung und Fuzzydechiffrierung von Informationen. Offenlegungsschrift DE 199 36 925 A 1. Deutsches Patent- und Markenamt; 2001]. The conceptual framework of that theory has been used by Nieto et al. [Nieto JJ, Torres A, Vázquez-Trasande MM. A metric space to study differences between polynucleotides. Appl Math Lett 2003;16:1289-94; Nieto JJ, Torres A, Georgiou DN, Karakasidis TE. Fuzzy polynucleotide spaces and metrics. Bull Math Biol 2006;68:703-25] and Torres et al. [Torres A, Nieto JJ. The fuzzy polynucleotide space: basic properties. Bioinformatics 2003;19:587-92; Torres A, Nieto JJ. Fuzzy logic in medicine and bioinformatics. J Biomed Biotechnol 2006;1-7 [Article ID 91908]] to create a completely different, 12-dimensional metric space which they have also called 'the fuzzy polynucleotide space'. In the present paper both spaces are compared.
MATERIAL AND METHOD
Both metric spaces are briefly outlined. Similarity and dissimilarity relationships between polynucleotide strings are measured in both spaces to compare their performance.
RESULTS
Nieto et al.'s and Torres et al.'s metric space measures the relationships between polynucleotide chains incorrectly. Structurally highly different polynucleotide sequences are misclassified as highly similar ones, and completely different sequences are misclassified as identical ones. For this reason their construct is to be considered as a device of misdiagnosis that bears "fuzzy polynucleotide space" as a misnomer.
Topics: Base Sequence; Fuzzy Logic; Humans; Models, Genetic; Nucleic Acid Conformation; Polynucleotides
PubMed: 17614264
DOI: 10.1016/j.artmed.2007.04.006 -
Archiv Der Pharmazie Aug 2019This mini-review describes the interaction between small molecules and RNA, in addition to its application either in treating RNA-associated diseases or detecting target... (Review)
Review
This mini-review describes the interaction between small molecules and RNA, in addition to its application either in treating RNA-associated diseases or detecting target molecules. In the case of RNA-associated disease treatment, the designed small molecules interact with RNA sites, forming adducts and providing successful therapeutic strategies over oligonucleotides. On the other hand, synthetically designed RNA moieties (aptamers) interact with target molecules like toxins, drugs, hormones; these interactions are useful in the detection, quantification or separation of these target moieties.
Topics: Aptamers, Nucleotide; Hormones; Pharmaceutical Preparations; Polynucleotides; RNA; Small Molecule Libraries; Toxins, Biological
PubMed: 31169327
DOI: 10.1002/ardp.201900062 -
Angewandte Chemie (International Ed. in... Nov 2023DNA nanotechnology provides an approach to create precise, tunable, and biocompatible nanostructures for biomedical applications. However, the stability of these...
DNA nanotechnology provides an approach to create precise, tunable, and biocompatible nanostructures for biomedical applications. However, the stability of these structures is severely compromised in biological milieu due to their fast degradation by nucleases. Recently, we showed how enzymatic polymerization could be harnessed to grow polynucleotide brushes of tunable length and location on the surface of DNA origami nanostructures, which greatly enhances their nuclease stability. Here, we report on strategies that allow for both spatial and temporal control over polymerization through activatable initiation, cleavage, and regeneration of polynucleotide brushes using restriction enzymes. The ability to site-specifically decorate DNA origami nanostructures with polynucleotide brushes in a spatiotemporally controlled way provides access to "smart" functionalized DNA architectures with potential applications in drug delivery and supramolecular assembly.
Topics: Polynucleotides; Nanostructures; DNA; Nanotechnology; Drug Delivery Systems; Nucleic Acid Conformation
PubMed: 37820028
DOI: 10.1002/anie.202311727 -
Biomaterials Aug 2014Delivery of polynucleotides into patient cells is a promising strategy for treatment of genetic disorders. Gene therapy aims to either synthesize desired proteins (DNA... (Review)
Review
Delivery of polynucleotides into patient cells is a promising strategy for treatment of genetic disorders. Gene therapy aims to either synthesize desired proteins (DNA delivery) or suppress expression of endogenous genes (siRNA delivery). Carriers constitute an important part of gene therapeutics due to limitations arising from the pharmacokinetics of polynucleotides. Non-viral carriers such as polymers and lipids protect polynucleotides from intra and extracellular threats and facilitate formation of cell-permeable nanoparticles through shielding and/or bridging multiple polynucleotide molecules. Formation of nanoparticulate systems with optimal features, their cellular uptake and intracellular trafficking are crucial steps for an effective gene therapy. Despite the great amount of experimental work pursued, critical features of the nanoparticles as well as their processing mechanisms are still under debate due to the lack of instrumentation at atomic resolution. Molecular modeling based computational approaches can shed light onto the atomic level details of gene delivery systems, thus provide valuable input that cannot be readily obtained with experimental techniques. Here, we review the molecular modeling research pursued on critical gene therapy steps, highlight the knowledge gaps in the field and providing future perspectives. Existing modeling studies revealed several important aspects of gene delivery, such as nanoparticle formation dynamics with various carriers, effect of carrier properties on complexation, carrier conformations in endosomal stages, and release of polynucleotides from carriers. Rate-limiting steps related to cellular events (i.e. internalization, endosomal escape, and nuclear uptake) are now beginning to be addressed by computational approaches. Limitations arising from current computational power and accuracy of modeling have been hindering the development of more realistic models. With the help of rapidly-growing computational power, the critical aspects of gene therapy are expected to be better investigated and direct comparison between more realistic molecular modeling and experiments may open the path for design of next generation gene therapeutics.
Topics: Drug Carriers; Endosomes; Gene Transfer Techniques; Genetic Therapy; Humans; Hydrogen-Ion Concentration; Lipids; Models, Molecular; Polymers; Polynucleotides; RNA, Small Interfering
PubMed: 24856107
DOI: 10.1016/j.biomaterials.2014.04.103 -
Tanpakushitsu Kakusan Koso. Protein,... Oct 1972
Review
Topics: Adenosine Diphosphate; Escherichia coli; Methods; Phosphorus Isotopes; Polynucleotides; Polyribonucleotide Nucleotidyltransferase; Proteins; RNA Nucleotidyltransferases
PubMed: 4567711
DOI: No ID Found -
The European Physical Journal. E, Soft... Mar 2021Hybrids formed by DNA/RNA and graphene family nanomaterials are considered as potentially useful multifunctional agents in biosensing and nanomedicine. In this work, we...
Hybrids formed by DNA/RNA and graphene family nanomaterials are considered as potentially useful multifunctional agents in biosensing and nanomedicine. In this work, we study the noncovalent interaction between double-stranded (ds) RNA, polyadenylic:polyuridylic acids (poly(A:U)) and graphene oxide/graphene (GO/Gr) using UV absorption spectroscopy and molecular dynamics (MD) simulations. RNA melting showed that relatively long ds-RNA is adsorbed onto GO (at an ionic strength of [Formula: see text]) at that a large fraction of RNA maintains the duplex structure. It was revealed that this fraction decreases over long time (during a few days), indicating a slow adsorption process of the long polymer. MD simulations showed that the adsorption of duplex (rA)[Formula: see text]: (rU)[Formula: see text] or (rA)[Formula: see text]: (rU)[Formula: see text] on graphene starts with the interaction between [Formula: see text]-systems of graphene and base pairs located at a duplex tail. In contrast to relatively long duplex (rA)[Formula: see text]: (rU)[Formula: see text] which keeps parallel arrangement along the graphene surface, the shorter one ((rA)[Formula: see text]: (rU)[Formula: see text]) always adopts a perpendicular orientation relative to graphene even in case of the initial parallel orientation. It was found out that (rA)[Formula: see text]: (rU)[Formula: see text] forms the stable hybrid with graphene keeping essential fraction of the duplex, while (rA)[Formula: see text]: (rU)[Formula: see text] demonstrates the duplex unzipping into two single strands with time. The interaction energies between adenine/uracil stacked with graphene as well between nucleotides in water environment were determined.
Topics: Adsorption; Graphite; Molecular Dynamics Simulation; Nanostructures; Osmolar Concentration; Poly A; Poly U; Polynucleotides; RNA, Double-Stranded; Surface Properties
PubMed: 33686498
DOI: 10.1140/epje/s10189-021-00030-z