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Physical Review Letters Jan 2001The structural property of a poly( dG-dC) or poly( dA-dT) nucleotide is investigated. At low force and room temperatures, the polymer takes on compact hairpin...
The structural property of a poly( dG-dC) or poly( dA-dT) nucleotide is investigated. At low force and room temperatures, the polymer takes on compact hairpin structures. An abrupt transition from hairpin to random coil occurs at certain critical forces, its high cooperativity is related to the unfavorable formation of hairpin and other kinds of looped structures. It is hence necessary to consider the enthalpic effects of single-stranded loops in realistic models of RNA folding. A possible new way to obtain the statistical weights of elementary nucleotide arrangements is by single-macromolecular mechanical measurements on specifically designed polynucleotides.
Topics: Base Pairing; DNA; Kinetics; Mathematics; Models, Molecular; Nucleic Acid Conformation; Poly dA-dT; Polydeoxyribonucleotides
PubMed: 11177830
DOI: 10.1103/PhysRevLett.86.356 -
Cancer Research Mar 1994We have constructed a DNA plasmid encoding the full length complementary DNA for human carcinoembryonic antigen (CEA) driven by the cytomegalovirus early...
We have constructed a DNA plasmid encoding the full length complementary DNA for human carcinoembryonic antigen (CEA) driven by the cytomegalovirus early promoter/enhancer (plasmid DNA encoding human CEA) and demonstrated that this plasmid can function as a polynucleotide vaccine. This polynucleotide vaccine induced humoral and/or cellular immune responses specific for human CEA in all 5 immunized mice. Lymphoblastic transformation data with the use of enriched T-cell populations detected the presence of CEA-specific memory T-cells in 3 of 5 mice. Lymphocytes from 2 of 5 mice had interleukin 2/interleukin 4 release in response to CEA. CEA specificity was confirmed by the absence of reactivity to a control antigen and lack of CEA reactivity among mice vaccinated with a control plasmid encoding chloramphenicol acetyltransferase. Four of 5 mice vaccinated with plasmid DNA encoding human CEA demonstrated anti-CEA antibody responses. This immune response compared favorably with a positive control group of mice immunized with vaccinia-CEA by a dose and schedule previously shown to induce immunoprotection and therapy against a human CEA expressing syngeneic murine colon carcinoma model. Studies are ongoing to establish the construct, dose, and schedule to elicit optimal CEA-specific immune response as well as immunoprotection and therapy against human CEA expressing syngeneic murine adenocarcinoma models.
Topics: Animals; Antibody Formation; Antibody Specificity; Carcinoembryonic Antigen; DNA, Complementary; Dose-Response Relationship, Drug; Humans; Immunization; Lymphocyte Activation; Lymphocytes; Lymphokines; Mice; Mice, Inbred C57BL; Plasmids; Polynucleotides; Radiometry; Vaccines, Synthetic
PubMed: 8118800
DOI: No ID Found -
Science (New York, N.Y.) May 1986The crystalline complex of pancreatic ribonuclease (RNase) with oligomers of d(pA)4 has been solved by x-ray diffraction methods and refined by standard procedures to a...
The crystalline complex of pancreatic ribonuclease (RNase) with oligomers of d(pA)4 has been solved by x-ray diffraction methods and refined by standard procedures to a conventional crystallographic R factor of 0.22 at 2.5 angstrom resolution. The asymmetric unit is a complex of one RNase molecule associated with four d(pA)4 oligomers. Although the DNA in this complex is segmented, and therefore shows some discontinuities, it nevertheless traces a continuous path 12 nucleotides in length that passes through the active site cleft of the enzyme and over the surface of the protein. The DNA makes a series of eight to nine electrostatic bonds between its phosphate groups and lysine and arginine residues on the protein, as well as specific chemical interactions at the active site. The path described by the sequence of nucleotides is likely to be that taken by an extended polynucleotide chain when it is bound by the enzyme.
Topics: Animals; Cattle; DNA; Models, Molecular; Polynucleotides; Protein Conformation; Ribonuclease, Pancreatic; X-Ray Diffraction
PubMed: 3961503
DOI: 10.1126/science.3961503 -
Journal of Molecular Biology Dec 1962
Topics: Humans; Polynucleotides; Protein Structure, Secondary
PubMed: 13989046
DOI: 10.1016/s0022-2836(62)80097-7 -
Journal of Bacteriology Jul 1971The deoxyribonucleic acid of F-like plasmids appear to share a high degree of nucleotide similarity with each other but are not highly related to I-like plasmids.
The deoxyribonucleic acid of F-like plasmids appear to share a high degree of nucleotide similarity with each other but are not highly related to I-like plasmids.
Topics: Centrifugation, Density Gradient; Chromosomes, Bacterial; DNA, Bacterial; Drug Resistance, Microbial; Escherichia coli; Genes; Genetics, Microbial; Hot Temperature; Hydroxyapatites; Molecular Weight; Nucleic Acid Hybridization; Polynucleotides; Sucrose; Thymine; Tritium
PubMed: 4935326
DOI: 10.1128/jb.107.1.372-374.1971 -
Biochemical and Biophysical Research... Aug 1967
Topics: Carbon Isotopes; Cellulose; Coliphages; Enzyme Induction; Ligases; Polynucleotides; Tritium
PubMed: 6052493
DOI: 10.1016/0006-291x(67)90353-1 -
Biochemical and Biophysical Research... Nov 1970
Topics: Cellulose; Cyanides; Drug Stability; Methods; Micrococcus; Nucleotides; Polynucleotides; Protein Binding; RNA Nucleotidyltransferases
PubMed: 5479296
DOI: 10.1016/0006-291x(70)90071-9 -
Journal of General Microbiology Nov 1974
Topics: Base Sequence; Carbon Radioisotopes; Cytosine; DNA, Bacterial; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Guanine; Molecular Weight; Nucleic Acid Hybridization; Polynucleotides; Thymine; Tritium
PubMed: 4612106
DOI: 10.1099/00221287-85-1-65 -
The Journal of Biological Chemistry Aug 1962
Topics: Adenine Nucleotides; Adenosine Triphosphate; Cell Nucleus; Nucleic Acids; Phosphates; Polynucleotides; Polyribonucleotides; Transferases
PubMed: 13889231
DOI: No ID Found -
Journal of Korean Medical Science Nov 2014The Rejuran® is a new filler product made from purified polynucleotides. Here we present data from an animal study and a clinical trial to examine the durability,... (Randomized Controlled Trial)
Randomized Controlled Trial
A phase III, randomized, double-blind, matched-pairs, active-controlled clinical trial and preclinical animal study to compare the durability, efficacy and safety between polynucleotide filler and hyaluronic acid filler in the correction of crow's feet: a new concept of regenerative filler.
The Rejuran® is a new filler product made from purified polynucleotides. Here we present data from an animal study and a clinical trial to examine the durability, efficacy and safety of the Rejuran® on crow's feet. For the animal study, 25 mice were divided into three groups: Group 1 received phosphate buffered saline (PBS); Group 2 were treated with Yvoire®; and Group 3 were treated with Rejuran®. The durability and efficacy of each treatment were assessed by microscopy and staining. In the clinical trial, 72 patients were randomized to receive Rejuran® treatment for crow's feet on one side and Yvoire-Hydro® on the contralateral side, at a ratio of 1:1. Repeated treatments were performed every two weeks for a total of three times, over a total of 12 weeks' observation. All injections and observations of efficacy and safety were performed by the same two investigators. In the animal study, the Rejuran® group showed similar durability and inflammatory response to the Yvoire® group. Upon efficacy assessment, the Rejuran® group showed the greatest elasticity and collagen composition, and a significant difference in skin surface roughness and wrinkle depth. In the clinical trial, the primary and secondary objective efficacy outcome measure showed no statistical significance between the two groups, and in safety outcomes there were no unexpected adverse effects. Our data suggest that the Rejuran®, as a new regenerative filler, can be useful to reduce wrinkles, by showing evidence for its efficacy and safety.
Topics: Adult; Animals; Dermatologic Surgical Procedures; Double-Blind Method; Elasticity; Female; Humans; Hyaluronic Acid; Injections, Intradermal; Male; Mice; Middle Aged; Polynucleotides; Skin; Skin Aging; Surgery, Plastic; Treatment Outcome; Wound Healing
PubMed: 25473210
DOI: 10.3346/jkms.2014.29.S3.S201