-
Proceedings of the National Academy of... Oct 1968
Topics: Adenosine Triphosphate; Animals; Bone Marrow; Cell Nucleus; Ligases; Molecular Weight; Phosphorus Isotopes; Polynucleotides; Rabbits; Spleen
PubMed: 5245999
DOI: 10.1073/pnas.61.2.680 -
Harvey Lectures
Topics: DNA; Escherichia coli; Genetic Code; Peptides; Polynucleotides; RNA; RNA, Transfer; Ribosomes
PubMed: 4875306
DOI: No ID Found -
Biophysical Journal Feb 2006Polynucleotide adsorption to negatively charged surfaces via divalent ions is extensively used in the study of biological systems. We analyze here the adsorption...
Polynucleotide adsorption to negatively charged surfaces via divalent ions is extensively used in the study of biological systems. We analyze here the adsorption mechanism via a self-consistent mean-field model that includes the pH effect on the surface-charge density and the interactions between divalent ions and surface groups. The adsorption is driven by the cooperative effect of divalent metal ion condensation along polynucleotides and their reaction with the surface groups. Although the apparent reaction constants are enhanced by the presence of polynucleotides, the difference between reaction constants of different divalent ions at the ideal condition explains why not all divalent cations mediate DNA adsorption onto anionic surfaces. Calculated divalent salt concentration and pH value variations on polynucleotide adsorption are consistent with atomic force microscope results. Here we use long-period x-ray standing waves to study the adsorption of mercurated-polyuridylic acid in a ZnCl2 aqueous solution onto a negatively charged hydroxyl-terminated silica surface. These in situ x-ray measurements, which simultaneously reveal the Hg and Zn distribution profiles along the surface normal direction, are in good agreement with our model. The model also provides the effects of polyelectrolyte line-charge density and monovalent salt on adsorption.
Topics: Adsorption; Cations, Divalent; Hydrogen-Ion Concentration; Mercury; Models, Chemical; Poly U; Polynucleotides; Silicon Dioxide; Static Electricity; X-Ray Diffraction; Zinc
PubMed: 16449197
DOI: 10.1529/biophysj.105.070649 -
Aesthetic Plastic Surgery Aug 2021Poly-L-lactic acid (PLLA) is widely used in tissue engineering. The natural polymer hyaluronic acid (HA) shows excellent biocompatibility and affects cell signaling,...
BACKGROUND
Poly-L-lactic acid (PLLA) is widely used in tissue engineering. The natural polymer hyaluronic acid (HA) shows excellent biocompatibility and affects cell signaling, proliferation, and differentiation. In addition, a polynucleotide (PN) induces cell growth of human skin fibroblasts and osteoblasts.
OBJECTIVE
In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
MATERIALS AND METHODS
The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement. Additionally, safety and efficacy of HA-PN/PLLA composite filler were conducted in in vitro and in vivo.
RESULTS
Analysis of PLLA microspheres revealed spherical surfaces and a narrower particle size distribution than that in PLLA filler. HA-PN/PLLA composite filler had higher viscosity and elasticity values and similar osmolality as compared to those of HA and PN fillers. The nontoxicity in in vitro and in vivo tests reflected that the composite filler may be safe for human use. In addition, the composite filler maintained a more stable volume than did HA filler for 24 weeks after administration in HWY/Slc hairless rats. Furthermore, the results support the effect of HA-PN/PLLA in restoring skin structure.
CONCLUSION
Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
LEVEL OF EVIDENCE III
This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
Topics: Cosmetic Techniques; Dermal Fillers; Hyaluronic Acid; Polyesters; Polynucleotides
PubMed: 33876290
DOI: 10.1007/s00266-021-02295-3 -
Polynucleotide analogs: acrylic acid and maleic acid copolymers of 1-vinyluracil and 9-vinyladenine.Biochemistry Nov 1975Radical-induced copolymerization of 1-vinyluracil and maleic anhydride gave, after hydrolysis, a polymer containing a 1:1 monomer ratio of 1-vinyluracil-maleic acid....
Radical-induced copolymerization of 1-vinyluracil and maleic anhydride gave, after hydrolysis, a polymer containing a 1:1 monomer ratio of 1-vinyluracil-maleic acid. Gamma-Ray-induced copolymerization of 1-vinyluracil with acrylic acid gave a polymer with a ratio of 1:1.7. Similar treatment of 9-vinlyadenine and acrylic acid resulted in a polymer with a 1:3.2 ratio. These three compounds are potent stimulants of poly (uridylic acid) coded polyphenylalanine synthesis in an in vitro cell free system purified from Escherichia coli MRE 600. The double-stranded polymer, poly(inosinic acid)-poly(cytidylic acid), also stimulates polyphenylalanine synthesis in this assay.
Topics: Adenine; Binding Sites; Escherichia coli; Kinetics; Nucleic Acid Conformation; Poly A; Poly I-C; Poly U; Polynucleotides; Protein Biosynthesis; Ribosomes; Spectrophotometry, Ultraviolet; Structure-Activity Relationship; Uracil; Vinyl Compounds
PubMed: 1101958
DOI: 10.1021/bi00693a017 -
Proceedings of the National Academy of... Jun 1976Ribosomal 30S protein S1 causes disruption of the secondary structure of certain pyrimidine-containing polynucleotides. Helical poly(U), poly(C, U), and neutral and...
Ribosomal 30S protein S1 causes disruption of the secondary structure of certain pyrimidine-containing polynucleotides. Helical poly(U), poly(C, U), and neutral and acidic poly(C) are stoichiometrically converted by S1 to structures indistinguishable from their partially or completely thermally denatured forms, as revealed by circular dichroism. Of the several double- and triple-stranded helical polynucleotides tested that contain one polypurine strand and at least one polypyrimidine strand, only the conformation of the DNA.RNA hybrid, poly(A)-poly(dT), is perturbed. In the presence of S1, this hybrid undergoes a transition to a new structure that has a circular dichroism spectrum unlike either the native or thermally denatured forms. Intercalated ethidium bromide is released from poly(A)-poly(dT) by S1, confirming the occurrence of a conformational rearrangement. The translation inhibitor, autintricarboxylic acid, completely inhibits the action of S1 on polypyrimidines, but has no effect on the conformational perturbation of poly(A(-poly(dT). The possible relation between these observations and the biological function of protein S1 is discussed.
Topics: Circular Dichroism; Escherichia coli; Ethidium; Nucleic Acid Conformation; Poly C; Poly T; Poly U; Polynucleotides; Ribosomal Proteins
PubMed: 778845
DOI: 10.1073/pnas.73.6.1824 -
Biopolymers Jan 1976
Topics: Absorption; Adenosine Monophosphate; Circular Dichroism; DNA; Mathematics; Methods; Oligonucleotides; Polynucleotides; RNA; Spectrum Analysis
PubMed: 1244896
DOI: 10.1002/bip.1976.360150110 -
Nature: New Biology Oct 1971
Topics: Animals; Base Sequence; Cattle; DNA; Nucleic Acid Conformation; Poly I-C; Polynucleotides; RNA; Scattering, Radiation; Thymus Gland
PubMed: 5287084
DOI: 10.1038/newbio233161a0 -
Biochimica Et Biophysica Acta Jan 1966
Topics: Adenine Nucleotides; Chemical Phenomena; Chemistry, Physical; Hydrogen-Ion Concentration; Nucleotidyltransferases; Polynucleotides; Ribonucleases; Spectrophotometry; Uracil Nucleotides
PubMed: 5936952
DOI: No ID Found -
The Journal of Biological Chemistry Aug 1966
Topics: Adenine Nucleotides; Micrococcus; Nucleotidyltransferases; Polynucleotides
PubMed: 5921782
DOI: No ID Found