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Annales de Dermatologie Et de... Oct 2011
Review
Topics: Combined Modality Therapy; Diagnosis, Differential; Embolization, Therapeutic; Extremities; Hemangioma, Capillary; Humans; Infant, Newborn; Klippel-Trenaunay-Weber Syndrome; Lasers, Dye; Leg Length Inequality; Livedo Reticularis; Low-Level Light Therapy; Lymphedema; Port-Wine Stain; Skin Diseases, Vascular; Skin Neoplasms; Sturge-Weber Syndrome; Telangiectasis
PubMed: 21978511
DOI: 10.1016/j.annder.2011.06.006 -
Histology and Histopathology May 2019Port wine stain (PWS) is characterized as a progressive dilatation of immature venule-like vasculatures which result from differentiation-impaired endothelial cells. In...
INTRODUCTION
Port wine stain (PWS) is characterized as a progressive dilatation of immature venule-like vasculatures which result from differentiation-impaired endothelial cells. In this study, we aimed to identify the major biological pathways accounting for the pathogenesis of PWS.
METHODS
Sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to identify differentially expressed proteins in PWS lesions, followed by confirmative studies with immunohistochemistry, immunoblot and transmission electron microscopy (TEM).
RESULTS
107 out of 299 identified proteins showed differential expressions in PWS lesions as compared to normal skin, mainly involving the functions of biosynthesis, membrane trafficking, cytoskeleton and cell adhesion/migration. The confirmative studies showed that expressions of membrane trafficking/exocytosis related proteins such as VAT1, IQGAP1, HSC70, clathrin, perlecan, spectrin α1 and GDIR1 were significantly increased in PWS blood vessels as compared to normal ones; while collagen subtypes 6A1 and 6A3 were decreased in PWS skin. Furthermore, TEM studies showed there is a significant upregulation of extracellular vesicle exocytosis from PWS blood vessels as compared to control.
CONCLUSIONS
The biological process of membrane trafficking and exocytosis is enhanced in PWS blood vessels. Our results imply that the extracellular vesicles released by lesional endothelial cells may act as potential intercellular signaling mediators to contribute to the pathogenesis of PWS.
Topics: Endothelial Cells; Exocytosis; Humans; Port-Wine Stain; Protein Transport; Up-Regulation
PubMed: 30302745
DOI: 10.14670/HH-18-051 -
The British Journal of Dermatology Jun 2001
Topics: Eczema; Female; Humans; Infant; Laser Therapy; Male; Port-Wine Stain
PubMed: 11422059
DOI: 10.1046/j.1365-2133.2001.04250.x -
Journal of Cosmetic Dermatology Jun 2008Because of the heterogeneity in port-wine stains, a number of them prove resistant to treatment with the pulsed-dye laser (PDL). A new variable pulse-duration PDL...
BACKGROUND AND OBJECTIVES
Because of the heterogeneity in port-wine stains, a number of them prove resistant to treatment with the pulsed-dye laser (PDL). A new variable pulse-duration PDL improves a refractory port-wine stain.
STUDY DESIGN/MATERIALS AND METHODS
A 26-year-old man with a congenital port-wine stain underwent eight treatments with the 1.5-ms pulse-duration PDL resulting in minimal improvement. A single treatment was then administered with the 3-ms pulse-duration PDL.
RESULTS
Initial results of treatment with the 3-ms pulse-duration PDL on this patient's port-wine stain show dramatic clearance in areas previously treated with significantly higher fluences, an identical spot size, but with a pulse duration half as long.
CONCLUSION
Vessel heterogeneity may require changing pulse durations once a port-wine stain or a portion of a port-wine stain becomes refractory to given pulse-duration.
Topics: Adult; Equipment Design; Humans; Laser Therapy; Lasers, Dye; Male; Port-Wine Stain; Radiation Dosage; Retreatment; Skin
PubMed: 18482019
DOI: 10.1111/j.1473-2165.2008.00378.x -
The Australasian Journal of Dermatology Nov 2011We present a novel case of pyogenic granuloma occurring within a port-wine stain in two sequential pregnancies at different sites. There was no history of precipitating...
We present a novel case of pyogenic granuloma occurring within a port-wine stain in two sequential pregnancies at different sites. There was no history of precipitating events such as trauma. We discuss why a pyogenic granuloma may occur within a port-wine stain and how pregnancy may increase the likelihood of this occurring.
Topics: Adult; Female; Granuloma, Pyogenic; Humans; Port-Wine Stain; Pregnancy; Pregnancy Complications; Skin Diseases; Young Adult
PubMed: 22070718
DOI: 10.1111/j.1440-0960.2010.00680.x -
Clinical and Experimental Dermatology May 2003
Topics: Adult; Eczema; Female; Follow-Up Studies; Humans; Port-Wine Stain; Recurrence
PubMed: 12780725
DOI: 10.1046/j.1365-2230.2003.01234.x -
International Journal of Dermatology Mar 2019
Topics: Adult; Dermoscopy; Diagnosis, Differential; Female; Genetic Diseases, X-Linked; Humans; Male; Port-Wine Stain; Skin; Skin Diseases, Vascular
PubMed: 30478941
DOI: 10.1111/ijd.14310 -
Journal of the American Academy of... Apr 2012Pulsed dye laser (PDL) is the gold standard for treatment of port-wine stain (PWS) birthmarks but multiple treatments are required and complete resolution is often not... (Randomized Controlled Trial)
Randomized Controlled Trial
BACKGROUND
Pulsed dye laser (PDL) is the gold standard for treatment of port-wine stain (PWS) birthmarks but multiple treatments are required and complete resolution is often not achieved. Posttreatment vessel recurrence is thought to be a factor that limits efficacy of PDL treatment of PWS. Imiquimod 5% cream is an immunomodulator with antiangiogenic effects.
OBJECTIVE
We sought to determine if application of imiquimod 5% cream after PDL improves treatment outcome.
METHODS
Healthy individuals with PWS (n = 24) were treated with PDL and then randomized to apply posttreatment placebo or imiquimod 5% cream for 8 weeks. Chromameter measurements (Commission Internationale de l'Eclairage L∗a∗b∗ colorspace) for 57 PWS sites (multiple sites per patient) were taken at baseline and compared with measurements taken 8 weeks posttreatment. The Δa∗ (change in erythema) and ΔE (difference in color between normal-appearing skin and PWS skin) were measured to quantify treatment outcome.
RESULTS
Two patients developed minor skin irritation. Other adverse effects were not noted. Average ∆a∗ was 0.43 for PDL + placebo sites (n = 25) and 1.27 for PDL + imiquimod sites (n = 32) (P value = .0294) indicating a greater reduction in erythema with imiquimod. Average ∆E was 2.59 for PDL + placebo and 4.08 for PDL + imiquimod (P value = .0363), again indicating a greater color improvement with imiquimod.
LIMITATIONS
Effects were evaluated after a single treatment and duration of effect is unknown.
CONCLUSION
Combined selective photothermolysis and antiangiogenic therapy may enhance PWS treatment efficacy.
Topics: Adjuvants, Immunologic; Adolescent; Adult; Aged; Aminoquinolines; Combined Modality Therapy; Female; Humans; Imiquimod; Lasers, Dye; Male; Middle Aged; Port-Wine Stain; Single-Blind Method; Young Adult
PubMed: 22244840
DOI: 10.1016/j.jaad.2011.11.958 -
Journal of Biomedical Optics Apr 2015Controllable and effective irradiation of lesions is among the key factors that affect the potency of photodynamic therapy (PDT). An optimization method for the...
Controllable and effective irradiation of lesions is among the key factors that affect the potency of photodynamic therapy (PDT). An optimization method for the irradiance distribution of treatment was proposed which can be used to improve the efficacy of PDT and allow more lesions to receive the desired irradiance level in a single therapy session. With the proposed digital illumination binocular treatment system, the preferred surface normal vectors, irradiation angles, as well as area and weight coefficients of lesions can be achieved and used as characteristic parameters to optimize the irradiation direction. Two port-wine stain phantom experiments were performed. The comparison of the illumination area between preoptimization and postoptimization showed that the proposed method can effectively guide the light source control, improve the distribution of light dose, and increase the effective treatment area.
Topics: Computer Simulation; Dermoscopy; Humans; Imaging, Three-Dimensional; Light; Lighting; Models, Biological; Photochemotherapy; Photosensitizing Agents; Port-Wine Stain; Radiation Dosage; Therapy, Computer-Assisted; Treatment Outcome
PubMed: 25909708
DOI: 10.1117/1.JBO.20.4.048004 -
Clinical and Experimental Dermatology Mar 2003
Topics: Adult; Glaucoma; Humans; Male; Port-Wine Stain
PubMed: 12653726
DOI: 10.1046/j.1365-2230.2003.01232_8.x