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Research in Veterinary Science Dec 2022The purpose of this study was to establish a reliable and reproducible method to isolate and cultivate canine corneal epithelial and stromal cells (cCECs and cCSCs). The...
BACKGROUND/OBJECTIVE
The purpose of this study was to establish a reliable and reproducible method to isolate and cultivate canine corneal epithelial and stromal cells (cCECs and cCSCs). The cells were subsequently used for in vitro testing of topically applied diluted povidone iodine (PVI).
METHODS
Corneas of dogs, euthanized for reasons unrelated to this study, were used to collect primary cCECs and cCSCs. Corneas were enzymatically digested and explants obtained using biopsy punches. Epithelial and stromal explants were separately taken into culture. Cell proliferation and migration was evaluated after incubation of cCECs and cCSCs with PVI in different concentrations (1, 2, or 5%) and with different exposure times (1, 3, or 10 min).
RESULTS
Solely incubation of 4 mm diameter corneal epithelial explants in a 48-well culture plate in full medium led to sufficient growth of cCECs. Up to four passages were achieved with a cell density of 10,000-20,000 cells/cm after dissociation of cells in trypsin for 8 min. Cell detachment and passaging for cCSCs were possible with almost every cornea and explant. Canine CSCs were less sensitive to PVI in all concentrations and over time than cCECs. Epithelial and stromal cell proliferation and migration decreased with increasing exposure times and PVI concentrations across all groups.
CONCLUSIONS
The described method is a straightforward and sound way to isolate and cultivate cCECs and cCSCs in vitro. Basic information on cCEC and cCSC migration and proliferation after incubation with PVI, was gathered. The results may provide a step towards an optimal preoperative antisepsis protocol for ophthalmic surgery in future.
Topics: Animals; Cell Proliferation; Cornea; Dogs; Epithelial Cells; Povidone-Iodine; Stromal Cells
PubMed: 35850012
DOI: 10.1016/j.rvsc.2022.04.017 -
Journal of Wound Care Jul 1998The iodine content of a tulle gras-type dressing medicated with povidone-iodine (Poviderm) has been measured and its potential efficacy in wound care explored by means...
The iodine content of a tulle gras-type dressing medicated with povidone-iodine (Poviderm) has been measured and its potential efficacy in wound care explored by means of laboratory models. Simple tests demonstrated the ready diffusibility and antibacterial activity of povidone-iodine. Wound models clearly showed that the limiting factor for useful dressing life is extent of exudation. It seems likely that this dressing would provide good topical antibacterial prophylaxis and may reduce the bacterial burden of colonised wounds. The dressing should help contain wound bacteria and thus assist infection control.
Topics: Anti-Bacterial Agents; Bandages; Chronic Disease; Diffusion; Drug Evaluation, Preclinical; Humans; Povidone-Iodine; Pseudomonas Infections; Staphylococcal Infections; Wound Infection
PubMed: 9791357
DOI: 10.12968/jowc.1998.7.7.332 -
Surgery, Gynecology & Obstetrics Nov 1983In this prospective, randomized study of 187 patients undergoing a variety of general surgical procedures, no significant difference could be demonstrated between those... (Clinical Trial)
Clinical Trial Comparative Study Randomized Controlled Trial
In this prospective, randomized study of 187 patients undergoing a variety of general surgical procedures, no significant difference could be demonstrated between those patients whose wounds were irrigated with normal saline solution and those whose wounds were irrigated with povidone-iodine. The potential of an alpha or a beta error is discussed.
Topics: Humans; Middle Aged; Povidone; Povidone-Iodine; Prospective Studies; Random Allocation; Saline Solution, Hypertonic; Surgical Wound Infection; Therapeutic Irrigation
PubMed: 6635913
DOI: No ID Found -
Acta Ophthalmologica May 2017
Topics: Anti-Infective Agents, Local; Humans; Intraoperative Period; Ophthalmic Solutions; Ophthalmologic Surgical Procedures; Povidone-Iodine; Surgical Wound Infection
PubMed: 27273448
DOI: 10.1111/aos.13144 -
Orthopedics 2024Povidone-iodine is a common antiseptic demonstrating success in reducing infection rates in primary arthroplasty; however, recent data suggest that its use in revision...
Povidone-iodine is a common antiseptic demonstrating success in reducing infection rates in primary arthroplasty; however, recent data suggest that its use in revision arthroplasty may increase infection rates. This study evaluated the effect of povidone-iodine solution on antibiotic cement and investigated the connection between povidone-iodine and increased infection rates in revision arthroplasty. Sixty antibiotic cement samples (ACSs) were formed using gentamicin-impregnated cement. The ACSs were divided into three groups: group A (n=20) was subject to a 3-minute povidone-iodine soak followed by a saline rinse; group B (n=20) underwent a 3-minute saline soak; and group C (n=20) underwent only a saline rinse. The antimicrobial activity of the samples was tested using a Kirby-Bauer-like assay using . The zone of inhibition (ZOI) was measured every 24 hours for 7 days. All groups possessed the greatest antimicrobial activity at 24 hours. Group C displayed a mass-corrected ZOI of 395.2 mm/g, which was statistically greater than the group B ZOI (313.2 mm/g, <.05) but not the group A ZOI (346.5 mm/g, >.05). All groups demonstrated a decrease in antimicrobial activity at 48 through 96 hours, with no significant difference at any time point. Prolonged soaking of antibiotic cement in a povidone-iodine or saline solution results in elution of the antibiotic into the irrigation solution, blunting initial antibiotic concentration. When using antibiotic cement, antiseptic soaks or irrigation should be focused prior to cementation. [. 2024;47(1):10-14.].
Topics: Humans; Povidone-Iodine; Anti-Bacterial Agents; Anti-Infective Agents, Local; Bone Cements; Therapeutic Irrigation
PubMed: 37341567
DOI: 10.3928/01477447-20230616-01 -
Lancet (London, England) Apr 1976
Topics: Blood Urea Nitrogen; Body Surface Area; Burns; Creatinine; Humans; Kidney; Povidone; Povidone-Iodine; Time Factors
PubMed: 58175
DOI: 10.1016/s0140-6736(76)92132-2 -
JAMA Dec 1980In 12 nonpregnant women, total iodine, protein-bound iodine, inorganic iodine, and thyroxine values were measured in serum before and 15, 30, 45 or 60 minutes after a...
In 12 nonpregnant women, total iodine, protein-bound iodine, inorganic iodine, and thyroxine values were measured in serum before and 15, 30, 45 or 60 minutes after a two-minute vaginal disinfection with povidone-iodine (Betadine). Only 15 minutes after application, serum iodine levels were raised and remained significantly elevated 30, 45 and 60 minutes after disinfection. Serum concentrations of total iodine and inorganic iodine were increased up to fivefold to 15-fold, respectively; during the relative short period of observation, thyroxine levels were not altered. An overload of iodine can suppress thyroid hormonogenesis, and the fetal and neonatal thyroid glands are especially sensitive. In pregnant women, vaginitis should not be treated with povidone-iodine because of the possible development of iodine-induced goiter and hypothyroidism in the fetus and newborn. The risk is especially high when povidone-iodine is used repeatedly.
Topics: Absorption; Female; Fetal Diseases; Fetus; Goiter; Humans; Hypothyroidism; Infant, Newborn; Maternal-Fetal Exchange; Povidone; Povidone-Iodine; Pregnancy; Vagina
PubMed: 7431610
DOI: No ID Found -
Journal of Perioperative Practice Mar 2012This paper presents a critical analysis of studies regarding the effectiveness of preoperative skin preparation solutions. The aim was to allow evidence based practice... (Review)
Review
This paper presents a critical analysis of studies regarding the effectiveness of preoperative skin preparation solutions. The aim was to allow evidence based practice at a local level.
Topics: Anti-Infective Agents, Local; Chlorhexidine; Humans; Povidone-Iodine; Preoperative Care; Skin
PubMed: 22493876
DOI: No ID Found -
Journal of Endodontics Aug 1975The clinical effectiveness of povidone-iodine as a disinfectant in teeth isolated by a rubber dam was compared to the effectiveness of isopropyl alcohol. The results... (Comparative Study)
Comparative Study
The clinical effectiveness of povidone-iodine as a disinfectant in teeth isolated by a rubber dam was compared to the effectiveness of isopropyl alcohol. The results showed no statistically significant difference between povidone-iodine or isopropyl alcohol for disinfecting intact enamel surfaces. When a scrub-type application of povidone-iodine was used, preliminary removal of plaque with pumice did not increase its effectiveness. Both agents were significantly less effective at 15 minutes after application than they were at 90 seconds after application.
Topics: 2-Propanol; Bacteria; Colony Count, Microbial; Dental Disinfectants; Dental Enamel; Humans; Povidone-Iodine; Root Canal Therapy
PubMed: 10697476
DOI: 10.1016/s0099-2399(75)80041-0 -
Retina (Philadelphia, Pa.) Jul 2023To investigate the temporal efficacy and sterility of povidone-iodine (PI) against bacteria associated with postinjection endophthalmitis in an in vitro study.
PURPOSE
To investigate the temporal efficacy and sterility of povidone-iodine (PI) against bacteria associated with postinjection endophthalmitis in an in vitro study.
METHODS
A single PI bottle was opened and tested for sterility and antibacterial efficacy. Povidone-iodine from the open bottle was inoculated onto a blood agar plate and evaluated for growth 24 hours later to test sterility; this was repeated for five consecutive days. To test for antibacterial efficacy, PI was instilled onto the surface of plates of Staphylococcus , Streptococcus , Enterococcus , Pseudomonas , and Haemophilus species. The plates were then evaluated 24 hours later for a clear zone of inhibition (i.e., no growth) where the PI was placed. This process was repeated for five consecutive days.
RESULTS
Sterility testing showed no growth of organisms on the blood agar plates for each of the five days of testing. For antibacterial efficacy testing, zones of inhibition of growth were seen on each plate of bacterial colonies only where PI was placed, for each of the five days of testing.
CONCLUSION
In this study, PI from an open bottle maintained its sterility and antibacterial efficacy for five days. These data could help support repeated use of the same bottle, reducing waste and costs for retina practices.
Topics: Humans; Povidone-Iodine; Anti-Infective Agents, Local; Agar; Bacteria; Anti-Bacterial Agents
PubMed: 36930891
DOI: 10.1097/IAE.0000000000003783