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The Australian Journal of Experimental... Dec 1956
Topics: Blood Coagulation; Blood Coagulation Factors; Blood Preservation; Humans; Preservation, Biological
PubMed: 13425978
DOI: 10.1038/icb.1956.53 -
Bibliotheca Haematologica 1958
Topics: Blood Preservation; Freezing; Humans; Preservation, Biological
PubMed: 13499285
DOI: 10.1159/000427128 -
Rivista Di Emoterapia Ed... 1955
Topics: Blood Banks; Blood Preservation; Erythrocytes; Humans; Leukocytes; Preservation, Biological
PubMed: 13311291
DOI: No ID Found -
Nihon Saikingaku Zasshi. Japanese... Mar 1975
Topics: Bacteriological Techniques; Freeze Drying; Freezing; Preservation, Biological
PubMed: 1100879
DOI: No ID Found -
Canadian Journal of Biochemistry and... Dec 1958
Topics: Adenosine; Blood Preservation; Erythrocytes; Humans; Inosine; Leadership; Nucleosides; Nucleotides; Preservation, Biological
PubMed: 13596903
DOI: No ID Found -
Surgical Forum 1953
Topics: Freeze Drying; Preservation, Biological; Transplantation
PubMed: 13122515
DOI: No ID Found -
Proteomics Oct 2009Sample degradation is a common problem in all types of proteomic analyses as it generates protein and peptide fragments that can interfere with analytical results. An...
Sample degradation is a common problem in all types of proteomic analyses as it generates protein and peptide fragments that can interfere with analytical results. An important step in preventing such artefacts is to preserve the native, intact proteome as early as possible during sample preparation prior to proteomic analysis. Using the budding yeast Saccharomyces cerevisiae, we have evaluated the effects of trichloroacetic acid (TCA) and thermal treatments prior to protein extraction as a means to minimise proteolysis. TCA precipitation is commonly used to inactivate proteases; thermal stabilisation is used to heat samples to approximately 95 degrees C to inactivate enzyme activity. The efficacy of these methods was also compared with that of protease inhibitors and lyophilisation. Sample integrity was assessed by 2-D PAGE and a selection of spots was identified by MS/MS. The analysis showed that TCA or thermal treatment significantly reduced the degree of degradation and that these pre-treatment protocols were more effective than treatment with either protease inhibitors or lyophilisation. This study establishes standardised sample preparation methods for the reproducible analysis of protein patterns by 2-D PAGE in yeast, and may also be applicable to other proteomic analyses such as gel-free-based quantitation methods.
Topics: Electrophoresis, Gel, Two-Dimensional; Preservation, Biological; Protein Stability; Proteome; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Tandem Mass Spectrometry; Temperature; Trichloroacetic Acid
PubMed: 19824011
DOI: 10.1002/pmic.200800945 -
The Lancet. Infectious Diseases Nov 2014
Topics: Biological Warfare Agents; Bioterrorism; Communicable Diseases; Containment of Biohazards; Disclosure; Humans; Microbiological Techniques; Preservation, Biological
PubMed: 25541049
DOI: 10.1016/S1473-3099(14)70979-X -
Revue D'hematologie 1946
Topics: Blood Transfusion; Humans; Preservation, Biological
PubMed: 20276278
DOI: No ID Found -
Nihon Saikingaku Zasshi. Japanese... Mar 1975
Topics: Fungi; Preservation, Biological
PubMed: 1236961
DOI: No ID Found