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Current Science Oct 1948
Topics: Pancreas; Preservation, Biological; Refrigeration
PubMed: 18101632
DOI: No ID Found -
Ecotoxicology and Environmental Safety Nov 2017Formaldehyde has been prominent in preserving biological tissues since the nineteenth century. Despite being admittedly harmful to health and to the environment, it is...
Formaldehyde has been prominent in preserving biological tissues since the nineteenth century. Despite being admittedly harmful to health and to the environment, it is still widely used. The Morphology Department of the University of Brasília - Brazil, applied the rethink, reduce, reuse, recycle and responsibility methodology to their activities in an effort to protect the health of laboratory workers and users, save resources and reduce damage to the environment. Here we evaluate the results obtained a decade after the implementation of this proposal (2005-2015). Formaldehyde was replaced by alcohol and glycerol solutions in corpse conservation. Over five thousand dollars in public funds that would have been destined to buying preserving substances were saved annually, and over a hundred thousand liters of water that would have been contaminated and thrown into the sewage system were spared. The environment used to implement the study was improved and anatomical parts kept for study had their lifespan extended. It is noteworthy that such simple adjustments could cause pronounced changes in laboratory activities. We would avoid contaminating billions of liters of water and it would be possible to save millions if similar practices were implemented in all educational institutions having similar routines.
Topics: Alcohols; Brazil; Cadaver; Conservation of Natural Resources; Embalming; Environmental Health; Fixatives; Formaldehyde; Glycerol; Humans; Preservation, Biological; Solutions
PubMed: 28783598
DOI: 10.1016/j.ecoenv.2017.07.072 -
Folia Microbiologica 1975Oomycetes and predacious Fungi imperfecti were preserved viable for four years by storage at 22 degrees C under paraffin oil. This method of culture preservation was...
Oomycetes and predacious Fungi imperfecti were preserved viable for four years by storage at 22 degrees C under paraffin oil. This method of culture preservation was checked on 52 species belonging to 4 orders and 13 genera.
Topics: Fungi; Mitosporic Fungi; Oils; Oomycetes; Paraffin; Preservation, Biological
PubMed: 1236823
DOI: 10.1007/BF02878118 -
Macromolecular Rapid Communications Aug 2013Monolithic aerogels can be easily obtained by drying physical gels formed by linear uncross-linked polymers. Preparation methods, structure, and properties of these... (Review)
Review
Monolithic aerogels can be easily obtained by drying physical gels formed by linear uncross-linked polymers. Preparation methods, structure, and properties of these physically cross-linked polymeric aerogels are reviewed, with particular emphasis to those whose cross-linking knots are crystallites and, more in particular, crystallites exhibiting nanoporous-crystalline forms. The latter aerogels present beside disordered amorphous micropores (typical of all aerogels) also all identical nanopores of the crystalline phases. Their outstanding guest transport properties combined with low material cost, robustness, durability, and ease of handling and recycle make these aerogels suitable for applications in chemical separations, purification, and storage as well as in biomedicine. Scientific, technological, and industrial perspectives for monolithic nanoporous-crystalline polymeric aerogels are also discussed.
Topics: Gels; Nanopores; Polymers; Porosity; Portraits as Topic; Preservation, Biological
PubMed: 23913316
DOI: 10.1002/marc.201300260 -
Blood Mar 2013Assessing messenger RNA (mRNA) and microRNA levels in peripheral blood cells may complement conventional parameters in clinical practice. Working with small, precious...
Assessing messenger RNA (mRNA) and microRNA levels in peripheral blood cells may complement conventional parameters in clinical practice. Working with small, precious samples requires optimal RNA yields and minimal RNA degradation. Several procedures for RNA extraction and complementary DNA (cDNA) synthesis were compared for their efficiency. The effect on RNA quality of freeze-thawing peripheral blood cells and storage in preserving reagents was investigated. In terms of RNA yield and convenience, quality quantitative polymerase chain reaction signals per nanogram of total RNA and using NucleoSpin and mirVana columns is preferable. The SuperScript III protocol results in the highest cDNA yields. During conventional procedures of storing peripheral blood cells at -180°C and thawing them thereafter, RNA integrity is maintained. TRIzol preserves RNA in cells stored at -20°C. Detection of mRNA levels significantly decreases in degraded RNA samples, whereas microRNA molecules remain relatively stable. When standardized to reference targets, mRNA transcripts and microRNAs can be reliably quantified in moderately degraded (quality index 4-7) and severely degraded (quality index <4) RNA samples, respectively. We describe a strategy for obtaining high-quality and quantity RNA from fresh and stored cells from blood. The results serve as a guideline for sensitive mRNA and microRNA expression assessment in clinical material.
Topics: Algorithms; Blood Cells; Blood Chemical Analysis; Blood Preservation; Blood Specimen Collection; Calibration; Genetic Techniques; Guanidines; Humans; MicroRNAs; Phenols; Polymerase Chain Reaction; Preservation, Biological; RNA Stability; RNA, Messenger; Reference Standards
PubMed: 23327925
DOI: 10.1182/blood-2012-06-438887 -
Theriogenology Mar 2019Biobanking is a rapidly growing industry, covering diverse fields such as human medicine, farm animal production, laboratory animals record keeping, and wildlife... (Review)
Review
Biobanking is a rapidly growing industry, covering diverse fields such as human medicine, farm animal production, laboratory animals record keeping, and wildlife conservation. Presently, biobanking is done almost exclusively by cryopreservation, followed by maintenance of the samples under liquid nitrogen. Cryopreservation has satisfactory efficiency but it comes with a host of problems, and the process is highly species-specific. Like in many other walks of life, we turn to Nature in search for better alternatives. Nature opted for controlled drying rather than water preservation via freezing when long-term preservation is desired, a strategy known as 'anhydrobiosis'. To achieve reversible drying, anhydrobiotic organisms utilise an assortment of protective materials, including disaccharides, late embryogenesis abundant proteins, anhydrin, heat shock protein, and more. Once dry, desiccation-tolerant organisms can survive extended periods of time and be resistant to extreme environmental stressors. Over the past 70 years researchers attempted applying this idea to preserve desiccation-sensitive mammalian cells in the dry form. At present dried cells mostly do not resume biological activity upon rehydration. The DNA, however, is often well preserved to allow utilisation in advanced reproductive techniques. Spermatozoa are by far the most commonly dried cell type, primarily from mice and bulls. A number of drying approaches have been applied, with freeze-drying taking the lead. To date offspring have been produced from dried spermatozoa in mouse, rat, hamster, rabbit, and horse. No offspring were produced from dried somatic cells. Desiccation experiences a sharp increase in interest and research output in recent years. Presented here is an overview of dry preservation, its possible applications, the open questions the field is still facing, and some suggested directions for the future.
Topics: Animals; Biological Specimen Banks; Desiccation; Male; Preservation, Biological; Spermatozoa
PubMed: 30508788
DOI: 10.1016/j.theriogenology.2018.11.027 -
Hema Jul 1945
Topics: Blood Transfusion; Humans; Preservation, Biological
PubMed: 20982122
DOI: No ID Found -
Molecular Ecology Resources Apr 2022Culture-independent survey techniques are fundamental tools when assessing plant microbiomes. These methods rely on DNA that is carefully preserved after collecting...
Culture-independent survey techniques are fundamental tools when assessing plant microbiomes. These methods rely on DNA that is carefully preserved after collecting samples to achieve meaningful results. Immediately freezing samples to -80°C after collection is considered one of the most robust methods for preserving samples before DNA extraction but is often impractical. Preservation solutions can solve this problem, but commercially available products are expensive, and there is limited data comparing their efficacy with other preservation methods. In this study, we compared the impact of three methods of sample preservation on plant microbiome surveys: (1) RNAlater, a proprietary preservative, (2) a home-made salt-saturated dimethyl sulphoxide preservation solution (DESS), and (3) freezing at -80°C. DESS-preserved samples, stored at room temperature for up to four weeks, did not show any significant differences to samples frozen at -80°C, while RNAlater inflated bacterial alpha diversity. Preservation treatments did not distinctively influence fungal alpha diversity. Our results demonstrate that DESS is a versatile and inexpensive preservative of DNA in plant material for diversity analyses of fungi and bacteria.
Topics: Bacteria; Freezing; Microbiota; Preservation, Biological; Specimen Handling
PubMed: 34695303
DOI: 10.1111/1755-0998.13538 -
Proceedings of the Royal Institution of... 1946
Topics: Desiccation; Food; Food, Preserved; Humans; Preservation, Biological
PubMed: 20282035
DOI: No ID Found -
Isotopes in Environmental and Health... Sep 2011Recent studies have shown that the stable carbon isotope compositions of dissolved inorganic carbon (δ(13)C(DIC)) of water samples preserved with HgCl(2) and CuSO(4)...
Recent studies have shown that the stable carbon isotope compositions of dissolved inorganic carbon (δ(13)C(DIC)) of water samples preserved with HgCl(2) and CuSO(4) vary. Furthermore, mercury and cuprum compounds are toxic to the human or biological system and require proper waste disposal. To test the effect of preservation on the δ(13)C value of DIC in different types of water samples, a set of water samples with different DIC concentrations was preserved using different methods, including preserving with inhibitors (CuSO(4) or HgCl(2)), preserving under frozen conditions, filtering through a 0.4 μ m paper filter, and the DIC species precipitated in the form of solid BaCO(3). Our results show that δ(13)C(DIC) values of the samples preserved with CuSO(4) and HgCl(2) become more positive with increased storage time. The δ(13)C(DIC) of the water samples preserved under frozen conditions and the precipitated DIC as BaCO(3) are also more positive than original water samples. However, the δ(13)C values were relatively stable for up to 90 days in all water samples filtered through the 0.4 μ m paper filter and stored under cool conditions (0-4 °C). Therefore, we suggest that the better method for the storage of water samples is to filter the samples through a 0.4 μ m paper filter while out in the field and preserve them under cool conditions, thereby avoiding the use of preservatives.
Topics: Barium; Carbon; Carbon Isotopes; Carbonates; Copper Sulfate; Filtration; Freezing; Humans; Mercuric Chloride; Preservation, Biological; Specimen Handling; Time Factors; Water
PubMed: 21809940
DOI: 10.1080/10256016.2011.598934