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Problemy Gematologii I Perelivaniia... Feb 1959
Topics: Blood Preservation; Humans; Leukocytes; Preservation, Biological
PubMed: 13645543
DOI: No ID Found -
Tubercle Mar 1979Mycobacteria stored at -70 degrees C retain 100% viability and maintain their definitive taxonomic, serologic, immunologic, and pathogenic properties. When shipped at...
Mycobacteria stored at -70 degrees C retain 100% viability and maintain their definitive taxonomic, serologic, immunologic, and pathogenic properties. When shipped at ambient temperatures, however, suspensions of all mycobacteria lose viability in transit, with those species having a narrow temperature range for growth (Mycobacterium tuberculosis and M. bovis) being most severely affected. In spite of these losses, all strains retain their definitive taxonomic properties. If care is taken in pre-testing and post-testing the microbial populations being preserved, mycobacteria are probably best shipped in the lyophilized state, and this procedure has been successfully used for several international studies.
Topics: Freezing; Mycobacterium; Preservation, Biological; Specimen Handling; Temperature; Time Factors
PubMed: 452120
DOI: 10.1016/0041-3879(79)90054-0 -
Surgery, Gynecology & Obstetrics Dec 1948
Topics: Blood Banks; Blood Transfusion; Humans; Organizations; Preservation, Biological
PubMed: 18120509
DOI: No ID Found -
Journal of Animal Science Aug 1993When the decision was made to euthanatize an acutely laminitic Thoroughbred broodmare, graduate students from the Department of Animal Sciences and Industry...
When the decision was made to euthanatize an acutely laminitic Thoroughbred broodmare, graduate students from the Department of Animal Sciences and Industry reconstructed the skeleton for use as a teaching tool. The reproductive and gastrointestinal tracts were removed and preserved in formalin. The hide, muscle, tendons, ligaments, and organs were removed, and the bones were boiled in water for > or = 48 h to remove all remaining tissue. After boiling, the bones were soaked in gasoline to remove fat from the marrow cavities and then soaked in a bleach/detergent mixture as a final cleaning step. The bones were allowed to dry for several weeks, then a semi-gloss clear lacquer was applied to aid in preservation. The bones were connected with 17-gauge wire and supported by two 1.91-cm galvanized steel rods on a mobile platform. The vertebral column was aligned on flexible copper tube with a 1.27-cm diameter. Additional support was provided for the head and neck by aluminum and steel rods extending from the front support. The final product is a complete, mobile skeleton that will be used as a teaching aid in equine classes. The skeleton serves a function for all levels of the cognitive learning domain. Examples of applications include memorization, identification, and location of bones; use in case studies for synthesis and demonstration of brainstorming efforts; and evaluation of joint ailments for more advanced levels of learning.
Topics: Animal Husbandry; Animals; Bone and Bones; Digestive System; Female; Genitalia, Female; Horses; Preservation, Biological; Teaching Materials
PubMed: 8376253
DOI: 10.2527/1993.7182270x -
Journal of the South African Veterinary... Mar 1978Wilted Tribulus terrestris plants were harvested from a camp in which geeldikkop had just previously broken out. The plants were treated in various ways in an attempt to... (Comparative Study)
Comparative Study
Wilted Tribulus terrestris plants were harvested from a camp in which geeldikkop had just previously broken out. The plants were treated in various ways in an attempt to preserve their toxicity. The only successful method of preservation found was rapid freezing of harvested material. Three kg of plants were kept frozen for six weeks and then dosed via rumen fistula to a sheep which subsequently developed characteristic symptoms and lesions of geeldikkop.
Topics: Animals; Female; Plant Poisoning; Plants, Toxic; Preservation, Biological; Refrigeration; Sheep; Sheep Diseases
PubMed: 702505
DOI: No ID Found -
Journal of Clinical Microbiology Sep 2010We describe a novel method of fungal slide preparation named "agar block smear preparation." A total of 510 agar block smears of 25 fungal strains obtained from culture...
We describe a novel method of fungal slide preparation named "agar block smear preparation." A total of 510 agar block smears of 25 fungal strains obtained from culture collections, 90 QC fungal strains, and 82 clinical fungal strains from our clinical microbiology laboratory, which included a total of 137 species of yeasts, molds, and thermal dimorphic fungi, were prepared and examined. In contrast to adhesive tape preparation, agar block smears preserved the native fungal structures, such as intact conidiophores of Aspergillus species and arrangements of conidia in Scopulariopsis brevicaulis. Furthermore, agar block smears allowed examination of fungal structures embedded in the agar, such as the ascomata with ascomal hairs in Chaetomium funicola; pycnidium of Phoma glomerata; the intercalary ovoidal chlamydospores arranged in chains of Fusarium dimerum; and the lateral, spherical chlamydospores arranged in pairs of Fusarium solani. After 1 year of storage, morphological integrity was found to have been maintained in 459 (90%) of the 510 agar block smears. After 3 years of storage, morphological integrity was found to have been maintained in 72 (71%) of the 102 smears prepared in 2006. Agar block smear preparation preserves the native fungal structures and allows long-term storage and examination of fungal structures embedded in the agar, hence overcoming the major drawbacks of adhesive tape preparation. The major roles of agar block smear should be diagnosis for difficult cases, accurate identification of fungal species for clinical management of patients and epidemiological studies, and long-term storage for transportation of slides and education purposes.
Topics: Agar; Fungal Structures; Fungi; Microscopy; Mycology; Preservation, Biological
PubMed: 20660221
DOI: 10.1128/JCM.00917-10 -
Mycologia 1977
Topics: Ascomycota; Freeze Drying; Microbiological Techniques; Preservation, Biological
PubMed: 342951
DOI: No ID Found -
Bollettino Della Societa Italiana Di... Sep 1958
Topics: Blood Preservation; Cholinesterases; Humans; Plasma; Preservation, Biological
PubMed: 13584614
DOI: No ID Found -
Rapid Communications in Mass... Apr 2019
Topics: Animals; Carbon Isotopes; Ethanol; Freezing; Humpback Whale; Nitrogen Isotopes; Preservation, Biological; Skin
PubMed: 30672617
DOI: 10.1002/rcm.8392 -
Transfusion Aug 2009
Topics: Animals; Erythrocytes; Humans; Mice; Preservation, Biological
PubMed: 19732402
DOI: 10.1111/j.1537-2995.2009.02298.x