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Microbial Pathogenesis Mar 2023This study is an investigation of anaerobic nitrite and fumarate reduction/respiration abilities of two characterised Prevotella species namely Prevotella nigrescens...
OBJECTIVE
This study is an investigation of anaerobic nitrite and fumarate reduction/respiration abilities of two characterised Prevotella species namely Prevotella nigrescens (SS6B) and Prevotella buccae (GS6B) isolated from the periodontal pockets of chronic periodontitis (ChP) patients.
METHODS
Isolation and identification of the periodontal bacteria from 20 patients showing clinical symptoms of ChP. Characterisation of anaerobic nitrite and fumarate reduction was done in P. nigrescens (SS6B) and P. buccae (GS6B) using reduction assays, inhibition assays with use of specific inhibitors, growth assays and enzyme activity assays. Degenerate PCR was used to detect and amplify nitrite reductase (nrfA) and fumarate reductase (frdA) gene sequences in these Prevotella isolates. In addition, molecular and in silico analysis of the amplified anaerobic reductase gene sequences was performed using NCBI conserved domain analysis, Interpro database and MegaX.
RESULTS
We provided experimental evidence for presence of active nitrite and fumarate reductase activities through enzyme activity, reduction, inhibitor and growth assays. Moreover, we were able to detect presence of 505 bps nrfA gene fragment and 400 bps frdA gene fragment in these Prevotella spp. These fragments show similarity to multiheme ammonia forming cytochrome c nitrite reductases and fumarate reductases flavoprotein subunit, respectively.
CONCLUSION
Anaerobic nitrite and fumarate respiration abilities in P. nigrescens and P. buccae isolates appear to be important for detoxification process and growth, respectively.
Topics: Humans; Chronic Periodontitis; Prevotella nigrescens; Nitrites; Succinate Dehydrogenase
PubMed: 36739100
DOI: 10.1016/j.micpath.2023.106022 -
Science (New York, N.Y.) Apr 2023Type VI CRISPR-Cas systems use RNA-guided ribonuclease (RNase) Cas13 to defend bacteria against viruses, and some of these systems encode putative membrane proteins that...
Type VI CRISPR-Cas systems use RNA-guided ribonuclease (RNase) Cas13 to defend bacteria against viruses, and some of these systems encode putative membrane proteins that have unclear roles in Cas13-mediated defense. We show that Csx28, of type VI-B2 systems, is a transmembrane protein that assists to slow cellular metabolism upon viral infection, increasing antiviral defense. High-resolution cryo-electron microscopy reveals that Csx28 forms an octameric pore-like structure. These Csx28 pores localize to the inner membrane in vivo. Csx28's antiviral activity in vivo requires sequence-specific cleavage of viral messenger RNAs by Cas13b, which subsequently results in membrane depolarization, slowed metabolism, and inhibition of sustained viral infection. Our work suggests a mechanism by which Csx28 acts as a downstream, Cas13b-dependent effector protein that uses membrane perturbation as an antiviral defense strategy.
Topics: CRISPR-Cas Systems; Cryoelectron Microscopy; Membrane Proteins; RNA, Viral; Bacterial Proteins; Endodeoxyribonucleases; CRISPR-Associated Proteins; RNA Cleavage; Bacteriophages; Bacteriophage lambda; Escherichia coli; Prevotella
PubMed: 37104586
DOI: 10.1126/science.abm1184 -
Archive of Clinical Cases 2023is a gram-negative obligate anaerobe mainly associated with infections of odontogenic origin. Non-oral monomicrobial infection by these obligate anaerobic bacteria is...
is a gram-negative obligate anaerobe mainly associated with infections of odontogenic origin. Non-oral monomicrobial infection by these obligate anaerobic bacteria is rare. Only a few cases of monomicrobial non-oral infections by have been reported in the literature. We are reporting a case of unilateral complicated pleural empyema in a patient with bronchial asthma infected by . Pleural fluid aerobic culture and blood culture reports were sterile. No acid-fast bacilli were detected by Acid Fast Bacilli (AFB) staining, and cartridge-based nucleic acid assay test (CBNAAT) reports were negative for . The isolate, was found susceptible to Metronidazole (MIC = 3 μg/ml) and resistant to Clindamycin (MIC = 256 μg/ml). In view of rising trends of antimicrobial resistance among anaerobes, it is recommended to perform anaerobic culture and sensitivity testing in clinically suspected cases of pleuropulmonary infection for appropriate diagnosis and optimal patient management. Clindamycin should be used with caution for empiric treatment.
PubMed: 38026109
DOI: 10.22551/2023.41.1004.10263 -
Anaerobe Feb 2020Mediastinitis is a well-known complication of open-heart surgery. Strictly anaerobic bacteria are rarely found in this condition, unlike in descending mediastinitis. We...
Mediastinitis is a well-known complication of open-heart surgery. Strictly anaerobic bacteria are rarely found in this condition, unlike in descending mediastinitis. We report the case of a mediastinitis due to Prevotella buccae after surgical replacement of the aortic valve and triple coronary artery bypass in an immunocompetent 76 year-old man. The bacteria were found in pure culture on blood samples and surgical samples. This case emphasizes the need to perform anaerobic cultures in case of sternal wound infection after open-heart surgery.
Topics: Aged; Anti-Bacterial Agents; Bacterial Infections; Biomarkers; Cardiac Surgical Procedures; Humans; Male; Mediastinitis; Postoperative Complications; Prevotella
PubMed: 31494261
DOI: 10.1016/j.anaerobe.2019.102097 -
European Journal of Vascular and... Feb 1998
Topics: Aged; Aneurysm, False; Bacteremia; Bacteroidaceae Infections; Fatal Outcome; Humans; Male; Prevotella; Renal Artery Obstruction
PubMed: 9551059
DOI: 10.1016/s1078-5884(98)80141-x -
Cureus May 2023Necrotizing infections of deep neck spaces are a group of life-threatening infectious diseases acquired through trauma or as a descending infection from an odontogenic...
Necrotizing infections of deep neck spaces are a group of life-threatening infectious diseases acquired through trauma or as a descending infection from an odontogenic source. The isolation of pathogens is unusual because of the anaerobic nature of the infection; however, one way to achieve this is through the use of automated microbiological methods like matrix-assisted laser desorption/ionization and time-of-flight (MALDI-TOF) following standard microbiology protocols for analyzing samples from potential anaerobic infections. We present a case of a patient without risk factors for descending necrotizing mediastinitis with isolation managed at the intensive care unit with a multidisciplinary team. We present our approach and how we successfully treat this complicated infection.
PubMed: 37398802
DOI: 10.7759/cureus.39703 -
Anaerobe Dec 2017Prevotella buccae is a Gram-negative anaerobic rod involved in some human infections. We report the first case of an infected breast cyst in a non-puerperal women due to...
Prevotella buccae is a Gram-negative anaerobic rod involved in some human infections. We report the first case of an infected breast cyst in a non-puerperal women due to this pathogen. A 53-year-old woman presented with pain and a cystic lesion in the right breast. Culture of abscess drainage resulted in isolation of P. buccae. High level of resistance to metronidazole was documented. Treatment with drainage and amoxicillin-clavulanate was established, and improvement of this infection was observed.
Topics: Abscess; Bacteroidaceae Infections; Breast Cyst; Combined Modality Therapy; Drainage; Drug Resistance, Bacterial; Female; Humans; Mass Spectrometry; Mastitis; Metronidazole; Microbial Sensitivity Tests; Middle Aged; Prevotella; Treatment Outcome
PubMed: 28866113
DOI: 10.1016/j.anaerobe.2017.08.015 -
International Journal of Systematic and... Mar 2006Eight strains of anaerobic Gram-negative bacilli isolated from infections of the skin and soft tissues were subjected to a comprehensive range of phenotypic and...
Eight strains of anaerobic Gram-negative bacilli isolated from infections of the skin and soft tissues were subjected to a comprehensive range of phenotypic and genotypic tests. 16S rRNA gene sequence analysis revealed the strains to constitute a homogeneous group, distinct from species with validly published names but related to a cluster including Prevotella buccae, Prevotella dentalis and Prevotella baroniae. A novel species, Prevotella bergensis sp. nov., is proposed to accommodate these strains. Prevotella bergensis is saccharolytic and produces acetic and succinic acids as end products of fermentation. The G + C content of the DNA of the type strain is 48 mol%. The type strain of Prevotella bergensis is 94067913T (= DSM 17361T = CCUG 51224T).
Topics: DNA, Ribosomal; Fatty Acids; Humans; Nucleic Acid Hybridization; Phenotype; Phylogeny; Prevotella; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Skin Diseases, Bacterial; Soft Tissue Infections
PubMed: 16514036
DOI: 10.1099/ijs.0.63888-0 -
Biologia Futura Dec 2021More than 6 billion bacteria and other microorganisms live in the adult oral cavity. As a result of any deleterious effect on this community, some microorganisms will...
More than 6 billion bacteria and other microorganisms live in the adult oral cavity. As a result of any deleterious effect on this community, some microorganisms will survive better than others, which may trigger pathogenic processes like caries, halitosis, gingivitis or periodontitis. Oral dysbiosis is among the most frequent human health hazards globally. Quality of life of patients deteriorates notably, while treatments are often unpleasant, expensive and irreversible, e.g. tooth loss. In the experiments reported here, we investigated the individual interactions between 8 pathogenic and 8 probiotic strains and a commercially available probiotic product. Almost all pathogens, namely Fusobacterium nucleatum, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Streptococcus mutans, Streptococcus oralis, Streptococcus gordonii, Enterococcus faecalis and Prevotella buccae are pathogens frequently occurring in the oral cavity. The used probiotic strains were Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus delbrueckii, Bifidobacterium thermophilum and two Streptococcus dentisani isolates. Using a modified agar diffusion method, we investigated capability of the probiotic bacteria to prevent the growth of the pathogenic ones in order to identify candidates for future therapeutic treatments. The results indicated successful bacteriocin production, i.e. growth inhibition, against every pathogenic bacterium by at least 5 probiotic strains.
Topics: Antibiosis; Humans; Mouth; Periodontitis; Probiotics; Virulence Factors
PubMed: 34554489
DOI: 10.1007/s42977-021-00091-3 -
Veterinary Microbiology May 2017Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study...
Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study aimed to detect species of the genera Porphyromonas and Prevotella present in the periodontal pocket of sheep with lesions deeper than 5mm (n=14) and in the gingival sulcus of animals considered periodontally healthy (n=20). The presence of microorganisms was evaluated by polymerase chain reaction (PCR) using specific primers for Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, and Prevotella tannerae. Prevalence and risk analysis were performed using Student's t-test and Spearman's correlation. Among the Prevotella and Porphyromonas species detected in the periodontal lesions of sheep, P. melaninogenica (85.7%), P. buccae (64.3%), P. gingivalis (50%), and P. endodontalis (50%) were most prevalent. P. gingivalis (15%) and P. oralis (10%) prevailed in the gingival sulcus. P. gulae and P. tannerae were not detected in the 34 samples studied. Data evaluation by t-test verified that occurrence of P. asaccharolytica, P. endodontalis, P. gingivalis, P. buccae, P. intermedia, P. melalinogenica, and P. nigrescens correlated with sheep periodontitis. The findings of this study will be an important contribution to research on pathogenesis of sheep periodontitis and development of its control measures.
Topics: Animals; Bacteria, Anaerobic; Biofilms; Periodontal Pocket; Periodontitis; Polymerase Chain Reaction; Porphyromonas; Prevotella; Sheep; Sheep Diseases
PubMed: 28619155
DOI: 10.1016/j.vetmic.2017.03.032