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Clinical and Experimental Dermatology Jul 2012Botryomycosis is an uncommon chronic suppurative granulomatous bacterial infection that can affect the skin and viscera. Clinically, lesions typically consist of small...
Botryomycosis is an uncommon chronic suppurative granulomatous bacterial infection that can affect the skin and viscera. Clinically, lesions typically consist of small tender nodules from which draining sinuses may develop to expel a purulent discharge. Histopathological features include characteristic aggregation of microorganisms (grain) within the inflammatory infiltrate. The commonest causative organisms are Staphylococcus aureus and Pseudomonas aeruginosa, of others. Botryomycosis resulting from Prevotella melaninogenica has not been reported previously. We report the case of a middle-aged patient with botryomycosis presenting as nasal cutaneous fistulas caused by P. melaninogenica, which was successfully treated with surgical intervention combined with systemic antibiotic treatment.
Topics: Anti-Bacterial Agents; Bacteroidaceae Infections; Cutaneous Fistula; Female; Humans; Middle Aged; Nasal Cavity; Nose Diseases; Prevotella melaninogenica; Treatment Outcome
PubMed: 22212025
DOI: 10.1111/j.1365-2230.2011.04257.x -
Oral Health & Preventive Dentistry 2011The purpose of this review was to summarise the fundamentals about black stain, its diagnosis and possible differential diagnoses as well as its microbiology and... (Review)
Review
The purpose of this review was to summarise the fundamentals about black stain, its diagnosis and possible differential diagnoses as well as its microbiology and therapy. In addition, various studies investigating the relationship between black stain and dental caries are examined. Many studies report lower caries prevalence in children with black stain, but this finding could not be confirmed by all authors. Also, a negative relation between degree of staining and caries severity has been described. Reasons for these results are not yet clear but it was speculated that they are related to the specific oral microflora described in black stain-affected individuals.
Topics: Actinomyces; Dental Caries; Dental Plaque; Dental Prophylaxis; Ferrous Compounds; Humans; Prevalence; Prevotella melaninogenica; Tooth Discoloration
PubMed: 21594205
DOI: No ID Found -
Research in Microbiology Dec 2005We report a bacterial isolate (Marseille isolate) recovered from the blood of a patient hospitalized in an intensive care unit, presenting with severe trauma, fever and...
We report a bacterial isolate (Marseille isolate) recovered from the blood of a patient hospitalized in an intensive care unit, presenting with severe trauma, fever and mechanical ventilation. Colonies appeared at 37 degrees C on blood agar after 72 h incubation. This isolate was a strictly anaerobic, Gram-negative rod phenotypically related to other Prevotella species described to date: non-motile, catalase-negative, oxidase-positive, non-glucose fermenting, resistant to vancomycin and susceptible to kanamycin. Cells exhibited a trilamellar membrane under electron microscopy. The fatty acid methyl ester profile was marginally related to that of Clostridium botulinum group A (distance: 26.27%) and Bifidobacterium bifidum GC subgroup B (distance: 26.38%). 16S rRNA gene sequence similarity was 90.0% with that of Prevotella oris and 89.1% with that of Prevotella melaninogenica. Partial rpoB gene sequence similarity was 84.5 and 86.4% with P. oris and P. melaninogenica, respectively. According to current standards, phenotypic traits, 16S rRNA and rpoB gene sequence analyses indicated that the Marseille isolate belonged to a previously unrecognized species of the genus Prevotella, and we propose classifying it in the new taxon "Prevotella massiliensis" sp. nov.
Topics: Adult; Bacteremia; Bacterial Proteins; Bacterial Typing Techniques; Bacteroidaceae Infections; Blood; Culture Media; DNA, Bacterial; DNA, Ribosomal; Genes, rRNA; Humans; Male; Molecular Sequence Data; Phenotype; Phylogeny; Prevotella; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 16085394
DOI: 10.1016/j.resmic.2005.05.008 -
FEMS Microbiology Letters Nov 2000We investigated the mechanism of the oxidative DNA damage induction by exposure to O(2) in Prevotella melaninogenica, a strict anaerobe. Flow cytometry with...
We investigated the mechanism of the oxidative DNA damage induction by exposure to O(2) in Prevotella melaninogenica, a strict anaerobe. Flow cytometry with hydroethidine and dichlorofluorescein diacetate showed that O(2) exposure generated O(2)*-) and H(2)O(2). Results of electron spin resonance with alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone and ethanol showed that O(2) exposure also induced *OH radical generation in P. melaninogenica loaded with FeCl(2) but not in samples without FeCl(2) loading. In P. melaninogenica, O(2) exposure increased 8-hydroxydeoxyguanosine (8OHdG), typical of oxidative DNA damage. Catalase inhibited the increase, but the *OH radical scavengers did not. Phenanthroline, a membrane-permeable Fe and Cu chelator, increased the 8OHdG induction. In FeCl(2)-loaded samples, induction of 8OHdG decreased. Addition of H(2)O(2) markedly increased 8OHdG levels. These results indicate that in P. melaninogenica, exposure to O(2) generated and accumulated O(2)* and H(2)O(2), and that a crypto-OH radical generated through H(2)O(2) was the active species in the 8OHdG induction.
Topics: 8-Hydroxy-2'-Deoxyguanosine; Anaerobiosis; DNA Damage; Deoxyguanosine; Electron Spin Resonance Spectroscopy; Flow Cytometry; Hydrogen Peroxide; Hydroxyl Radical; Oxygen; Prevotella melaninogenica; Reactive Oxygen Species; Superoxides
PubMed: 11040441
DOI: 10.1111/j.1574-6968.2000.tb09371.x -
The Journal of Emergency Medicine Sep 2017
Topics: Aged; Anti-Bacterial Agents; Combined Modality Therapy; Debridement; Diagnosis, Differential; Electrocardiography; Exanthema; Firmicutes; Fournier Gangrene; Groin; Humans; Male; Prevotella melaninogenica; Shock; Therapeutic Irrigation
PubMed: 28268120
DOI: 10.1016/j.jemermed.2016.12.008 -
Veterinary Microbiology May 2017Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study...
Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study aimed to detect species of the genera Porphyromonas and Prevotella present in the periodontal pocket of sheep with lesions deeper than 5mm (n=14) and in the gingival sulcus of animals considered periodontally healthy (n=20). The presence of microorganisms was evaluated by polymerase chain reaction (PCR) using specific primers for Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, and Prevotella tannerae. Prevalence and risk analysis were performed using Student's t-test and Spearman's correlation. Among the Prevotella and Porphyromonas species detected in the periodontal lesions of sheep, P. melaninogenica (85.7%), P. buccae (64.3%), P. gingivalis (50%), and P. endodontalis (50%) were most prevalent. P. gingivalis (15%) and P. oralis (10%) prevailed in the gingival sulcus. P. gulae and P. tannerae were not detected in the 34 samples studied. Data evaluation by t-test verified that occurrence of P. asaccharolytica, P. endodontalis, P. gingivalis, P. buccae, P. intermedia, P. melalinogenica, and P. nigrescens correlated with sheep periodontitis. The findings of this study will be an important contribution to research on pathogenesis of sheep periodontitis and development of its control measures.
Topics: Animals; Bacteria, Anaerobic; Biofilms; Periodontal Pocket; Periodontitis; Polymerase Chain Reaction; Porphyromonas; Prevotella; Sheep; Sheep Diseases
PubMed: 28619155
DOI: 10.1016/j.vetmic.2017.03.032 -
Current Microbiology May 2006Prevotella intermedia and Prevotella nigrescens are often regarded as principal causes of acute dentoalveolar infection; however, other species within the genus are also...
Prevotella intermedia and Prevotella nigrescens are often regarded as principal causes of acute dentoalveolar infection; however, other species within the genus are also known to be associated with such infection. The aim of this study was to determine the in vitro proteolytic activity of these different Prevotella species that have been implicated with dentoalveolar infection. A total of 234 strains were obtained from pus specimens from dentoalveolar infection and from the plaque of healthy volunteers. Prevotella loescheii, Prevotella oralis, Prevotella melaninogenica, Prevotella buccae, and Prevotella denticola were all shown to have a proteolytic activity (8.5-10.5 x 10(-8) A-units) lower than that of P. intermedia and P. nigrescens (21.1-23.5 x 10(-8) A-units). In the case of P. loescheii, P. melaninogenica, and P. intermedia, the level of proteolytic activity for clinical strains was significantly (P < 0.05) higher than that recorded for commensal strains. Proteolytic activity for all species of Prevotella examined was inhibited by N-ethylmaleimide and phenymethylsulfonyl fluoride. This study suggests that Prevotella species associated with oral purulent infection produce cysteine and serine proteinases and that in certain species of Prevotella, the strains involved in infection exhibit higher proteolytic activity when compared with strains from healthy sites.
Topics: Bacteroidaceae Infections; Cysteine Endopeptidases; Humans; Periodontitis; Prevotella; Prevotella intermedia; Serine Endopeptidases
PubMed: 16586023
DOI: 10.1007/s00284-005-0261-1 -
Clinical Infectious Diseases : An... Jun 1995
Comparative Study
Topics: Genes, Bacterial; Haemophilus influenzae; Humans; Immunoglobulin A; Neisseria gonorrhoeae; Prevotella; Serine Endopeptidases
PubMed: 7548543
DOI: 10.1093/clinids/20.supplement_2.s169 -
Clinical Infectious Diseases : An... Jun 1995
Topics: Adult; Bacterial Typing Techniques; Child, Preschool; Female; Humans; Infant; Microbial Sensitivity Tests; Mouth; Penicillins; Prevotella melaninogenica; beta-Lactamases
PubMed: 7548599
DOI: 10.1093/clinids/20.supplement_2.s364 -
Microbiology Spectrum Sep 2023Bacterial infections in the lungs of persons with cystic fibrosis are typically composed of multispecies biofilm-like communities, which modulate clinically relevant...
Bacterial infections in the lungs of persons with cystic fibrosis are typically composed of multispecies biofilm-like communities, which modulate clinically relevant phenotypes that cannot be explained in the context of a single species culture. Most analyses to date provide a picture of the transcriptional responses of individual pathogens; however, there is relatively little data describing the transcriptional landscape of clinically relevant multispecies communities. Harnessing a previously described cystic fibrosis-relevant, polymicrobial community model consisting of and , we performed an RNA-Seq analysis on the biofilm population to elucidate the transcriptional profiles of the community grown in artificial sputum medium (ASM) as compared to growth in monoculture, without mucin, and in fresh medium supplemented with tobramycin. We provide evidence that, although the transcriptional profile of is community agnostic, the transcriptomes of and are community aware. Furthermore, and are transcriptionally sensitive to the presence of mucin in ASM, whereas and largely do not alter their transcriptional profiles in the presence of mucin when grown in a community. Only shows a robust response to tobramycin. Genetic studies of mutants altered in community-specific growth provide complementary data regarding how these microbes adapt to a community context. IMPORTANCE Polymicrobial infections constitute the majority of infections in the cystic fibrosis (CF) airway, but their study has largely been neglected in a laboratory setting. Our lab previously reported a polymicrobial community that can help explain clinical outcomes in the lungs of persons with CF. Here, we obtained transcriptional profiles of the community versus monocultures to provide transcriptional information about how this model community responds to CF-related growth conditions and perturbations. Genetic studies provide complementary functional outputs to assess how the microbes adapt to life in a community.
PubMed: 37772884
DOI: 10.1128/spectrum.02201-23