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The Biochemical Journal Feb 19681. The reactions of beta-propiolactone with amino acids were investigated under various conditions of pH and temperature to find those under which the reagent acted with...
1. The reactions of beta-propiolactone with amino acids were investigated under various conditions of pH and temperature to find those under which the reagent acted with specificity. 2. At pH9.0 and 22 degrees , after 15min. of reaction, at least 85% of each amino acid had reacted, methionine and cystine being the most reactive. 3. At pH7.0 and 22 degrees most amino acids reacted; methionine, cystine and histidine reacted almost entirely, and proline and lysine to a significantly smaller extent. 4. At pH3.0 and 22 degrees further specificity was obtained; methionine and cystine were the only reactive amino acids. 5. Reaction at pH3.0 and 0 degrees was specific for methionine; it was the only amino acid modified even after 145hr. of reaction.
Topics: Amino Acids; Autoanalysis; Chemical Phenomena; Chemistry; Cold Temperature; Cystine; Histidine; Hydrogen-Ion Concentration; Isoleucine; Lactones; Leucine; Lysine; Methionine; Phenylalanine; Proline; Tyrosine
PubMed: 5637366
DOI: 10.1042/bj1060829 -
Fish & Shellfish Immunology Feb 2016Herpesviral haematopoietic necrosis (HVHN) of gibel carp (Carassius gibelio) is a newly emerged infectious disease caused by cyprinid herpesvirus 2 (CyHV-2) and has...
Herpesviral haematopoietic necrosis (HVHN) of gibel carp (Carassius gibelio) is a newly emerged infectious disease caused by cyprinid herpesvirus 2 (CyHV-2) and has caused huge economic losses in aquaculture operations. Currently, no effective methods are available for the control of the disease. In this study, β-propiolactone inactivated cyprinid herpesvirus 2 (CyHV-2) vaccine was prepared, and the immune response and protection in cultured gibel carp after vaccination was thoroughly investigated. This included blood cell counting and classification, phagocytic activity, lysozyme and superoxide dismutase activity, neutralizing antibody titration, immune gene expression analysis, and determination of the relative percent survival in vaccinated gibel carp. The results of blood cell counts indicated that the numbers of the red and white blood cells in the peripheral blood of immunized gibel carp increased significantly at day 4 and day 7 after vaccination (p < 0.01). The differential leukocyte count of neutrophils and monocytes were significantly different compared to the control group at day 4 and 7 and the percentage of lymphocytes reached a peak at day 21. The phagocytic percentage and phagocytic index peaked at day 4 post-vaccination. The lysozyme activity and superoxide dismutase activity were significantly increased compared to the control group (p < 0.01). The serum neutralizing antibody titer peaked (203.03 ± 13.44) at day 21. The qPCR analysis revealed that the expression of the immune genes interlukin 11 and complement component C3 were significantly up-regulated in the immunized group. The challenge test demonstrated that the immunized group had a relative survival rate of 71.4%. These results indicate that the inactivated CyHV-2 vaccine induced both non-specific and specific anti-viral immune responses that resulted in significant protection against HVHN disease and mortality in gibel carp.
Topics: Animals; Cyprinidae; DNA Virus Infections; DNA Viruses; Fish Diseases; Immunity, Humoral; Immunity, Innate; Propiolactone; Vaccines, Inactivated; Viral Vaccines
PubMed: 26772479
DOI: 10.1016/j.fsi.2016.01.003 -
Biochemical Pharmacology Dec 1963
Topics: Chemical Phenomena; Chemistry; Deoxyguanine Nucleotides; Guanine Nucleotides; Guanosine; Lactones; Nucleosides; Propiolactone; RNA; Research
PubMed: 14096434
DOI: 10.1016/0006-2952(63)90216-8 -
Journal of Biomaterials Science.... 2001A delivery system for vanadium was developed using poly(beta-propiolactone) (PbetaPL) films. The release kinetics of a complex of vanadium (IV) with aspirin (VOAspi) was...
A delivery system for vanadium was developed using poly(beta-propiolactone) (PbetaPL) films. The release kinetics of a complex of vanadium (IV) with aspirin (VOAspi) was evaluated with films prepared from polymers of different molecular weights, as well as with variable drug load. A sustained release of vanadium over 7 days was achieved. The drug release kinetics depends on contributions from two factors: (a) diffusion of the drug; and (b) erosion of the PbetaPL film. The experimental data at an early stage of release were fitted with a diffusion model, which allowed determination of the diffusion coefficient of the drug. VOAspi does not show strong interaction with the polymer, as demonstrated by the low apparent partition coefficient (approximately 10(-2)). UMR106 osteosarcoma cells were used as a model to evaluate the anticarcinogenic effects of the VOAspi released from the PbetaPPL film. VOAspi-PbetaPL film inhibited cell proliferation in a dose-response manner and induced formation of approximately half of the thiobarbituric acid reactive substances (TBARS), an index of lipid peroxidation. compared to that with free VOAspi in solution. The unloaded PbetaPL film did not generate cytotoxicity, as evaluated by cell growth and TBARS. Thus, the polymer-embedded VOAspi retained the antiproliferative effects showing lower cytotoxicity than the free drug. Results with VOAspi-PbetaPL films suggest that this delivery system may have promising biomedical and therapeutic applications.
Topics: Animals; Aspirin; Bone Neoplasms; Cell Division; Drug Combinations; Drug Delivery Systems; Kinetics; Lipid Peroxidation; Osteoblasts; Osteosarcoma; Oxidative Stress; Propiolactone; Rats; Thiobarbituric Acid Reactive Substances; Tumor Cells, Cultured; Vanadium; Water
PubMed: 11787522
DOI: 10.1163/156856201753252499 -
Journal of Medical Virology Jun 1985A beta-propiolactone/ultraviolet irradiation procedure (beta PL/UV) has been evaluated for its ability to inactivate 30,000 chimpanzee infectious doses of the Hutchinson...
A beta-propiolactone/ultraviolet irradiation procedure (beta PL/UV) has been evaluated for its ability to inactivate 30,000 chimpanzee infectious doses of the Hutchinson strain of non-A, non-B (NANB) virus. The chimpanzees were inoculated with plasma to which this dose of the titrated virus had been added prior to application of the beta PL/UV process in accordance with a procedure used for licensed blood derivatives in Germany. Neither animal developed hepatitis. When subsequently challenged with the same contaminated plasma, which had not been sterilized, both animals promptly developed typical NANB hepatitis. This study extends the high (approximately 10(7)-fold) process efficiency of the beta PL/UV procedure previously reported for hepatitis B virus to a blood-borne NANB virus.
Topics: Animals; Female; Hepatitis C; Hepatitis Viruses; Lactones; Microscopy, Electron; Pan troglodytes; Plasma; Propiolactone; Ultraviolet Rays
PubMed: 3925077
DOI: 10.1002/jmv.1890160204 -
Journal of Medical Virology Nov 1988beta-propiolactone (beta-PL) treatment has been evaluated for its ability to inactivate 10(3.5) chimpanzee infectious doses (CID50) of the Hutchinson strain of hepatitis...
beta-propiolactone (beta-PL) treatment has been evaluated for its ability to inactivate 10(3.5) chimpanzee infectious doses (CID50) of the Hutchinson strain of hepatitis non-A, non-B virus (HNANBV). Two chimpanzees were inoculated with a beta-PL-treated immunoglobulin solution to which this dose of the titrated virus had been added prior to beta-PL treatment. beta-PL treatment was performed in accordance with the production procedure used for a licensed intravenous immunoglobulin preparation. Neither animal developed hepatitis. When subsequently challenged with the same spiked immunoglobulin solution that had not been beta-PL treated, both animals developed clear-cut hepatitis non-A, non-B. The results of this experiment demonstrate that beta-PL treatment is effective for the inactivation of hepatitis non-A, non-B virus in intravenous immunoglobulin.
Topics: Animals; Antiviral Agents; Drug Contamination; Drug Evaluation, Preclinical; Female; Hepatitis C; Hepatitis Viruses; Hepatitis, Viral, Human; Immunoglobulins; Injections, Intravenous; Lactones; Pan troglodytes; Propiolactone
PubMed: 3144576
DOI: 10.1002/jmv.1890260302 -
Nature Nov 1958
Topics: Anti-Infective Agents, Local; Clostridium perfringens; Propiolactone; Vaccination; Vaccines
PubMed: 13590278
DOI: 10.1038/1821216a0 -
Journal of Immunological Methods Dec 1986beta-Propiolactone (BPL) inactivates LAV/HTLV III, the retrovirus associated with acquired immune deficiency syndrome (AIDS). Addition to specimens from patients with...
beta-Propiolactone (BPL) inactivates LAV/HTLV III, the retrovirus associated with acquired immune deficiency syndrome (AIDS). Addition to specimens from patients with suspected AIDS or antibodies to LAV/HTLV III could reduce any occupational risk to laboratory staff. This study demonstrates that BPL treatment does not significantly affect the immunological analyses commonly required on these patients, namely measurements of serum immunoglobulins, complement components C3 and C4 and other serum proteins, detection of autoantibodies and estimations of T lymphocyte subpopulations.
Topics: Acquired Immunodeficiency Syndrome; Antibodies, Viral; HIV; Immunoassay; Lactones; Lymphocytes; Propiolactone
PubMed: 3640791
DOI: 10.1016/0022-1759(86)90324-8 -
Journal of Clinical Pathology Apr 1993Serum samples from 30 HIV seronegative patients were treated with beta propiolactone (BPL) to determine whether BPL interfaces with ELISA for specific antibodies against...
Serum samples from 30 HIV seronegative patients were treated with beta propiolactone (BPL) to determine whether BPL interfaces with ELISA for specific antibodies against protein and carbohydrate antigens. BPL had no discernible effect on specific antibody measurements by ELISA. With the measuring need for specific antibody measurements in the management of HIV seropositive patients, it is reassuring that this laboratory safety measure does not impair the reliability of results.
Topics: Antibodies, Bacterial; Bacterial Capsules; Bacterial Vaccines; Diphtheria Toxoid; Enzyme-Linked Immunosorbent Assay; HIV Infections; Haemophilus Vaccines; Humans; Pneumococcal Vaccines; Polysaccharides, Bacterial; Propiolactone; Tetanus Toxoid
PubMed: 8496395
DOI: 10.1136/jcp.46.4.368 -
Archives of Virology 1976
Topics: Culture Techniques; Hemagglutinins, Viral; Hemolysin Proteins; Lactones; Neuraminidase; Newcastle disease virus; Propiolactone; RNA, Viral; RNA-Dependent RNA Polymerase; Transcription, Genetic; Viral Proteins
PubMed: 999518
DOI: 10.1007/BF01317878