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Lancet (London, England) Jan 1970
Topics: Animals; Blood Vessels; Central Nervous System; Chemical Phenomena; Chemistry; Female; Humans; Lipid Metabolism; Male; Muscle Contraction; Prostaglandins; Solubility; Uterus
PubMed: 4189014
DOI: No ID Found -
Journal of the American Chemical Society Oct 1973
Topics: Cyclization; Indicators and Reagents; Methods; Models, Chemical; Molecular Conformation; Prostaglandins; Stereoisomerism; Temperature
PubMed: 4746282
DOI: 10.1021/ja00801a066 -
American Journal of Obstetrics and... Feb 1986Previously we reported that the human fetal adrenal gland secreted various prostaglandins and that prostaglandin secretion was inhibited by endogenously synthesized... (Comparative Study)
Comparative Study
Previously we reported that the human fetal adrenal gland secreted various prostaglandins and that prostaglandin secretion was inhibited by endogenously synthesized glucocorticoids. Furthermore, we reported that the neocortex secreted larger quantities of prostaglandins than did fetal zone tissue and that the pattern of secretion of prostaglandins of the two zones differed. In the present investigation the rate and pattern of prostaglandin secretion by adrenal tissue of anencephalic fetuses were assessed and compared to either whole or separated zones of human fetal adrenal tissue. The rate of prostaglandin secretion into the culture medium was determined by measuring prostaglandin E2 or prostaglandin F2 alpha with use of specific radioimmunoassays in media collected at 24-hour intervals. The rate of prostaglandin E2 secretion by adrenal glands obtained from two anencephalic fetuses declined rapidly from 1.71 +/- 0.65 ng/mg-1 of protein per 24 hours and 0.82 +/- 0.46 ng/mg-1 of protein per 24 hours on day 1, respectively, to 0.36 +/- 0.03 and 0.12 +/- 0.04 ng/mg-1 of protein per 24 hours by the fifth day in culture. The rate and pattern of prostaglandin F2 alpha secretion by anencephalic tissue was similar to that of prostaglandin E2. The pattern of prostaglandin secretion by anencephalic adrenal tissue was similar to that observed in neocortex tissue, but the rate of prostaglandin secretion was less. When the rates of prostaglandin secretion by anencephalic and neocortex adrenal tissue were compared to the rates of secretion of cortisol, an inverse relationship was observed. Finally, when whole human fetal adrenal glands or anencephalic tissues were incubated in the presence of adrenocorticotropic hormone, dexamethasone, metyrapone, or SU 10603, the data obtained seemed to suggest that the rate of prostaglandin secretion was regulated in both tissues by endogenously synthesized glucocorticosteroids. In summary, the pattern of secretion of prostaglandins by the anencephalic adrenal gland was similar to that of neocortex tissue, and the rate of secretion of prostaglandins was inhibited by endogenously synthesized cortisol.
Topics: Adrenal Cortex; Adrenal Glands; Anencephaly; Culture Media; Culture Techniques; Dinoprost; Dinoprostone; Fetus; Humans; Hydrocortisone; Prostaglandins; Prostaglandins E; Prostaglandins F; Radioimmunoassay; Secretory Rate
PubMed: 3456203
DOI: 10.1016/0002-9378(86)90674-5 -
Wiener Zeitschrift Fur Innere Medizin... 1973
Topics: Adult; Animals; Blood Coagulation; Blood Platelets; Carbohydrate Metabolism; Cardiovascular System; Digestive System; Female; Genitalia, Female; Genitalia, Male; Guinea Pigs; Humans; Kidney; Lipid Metabolism; Male; Mice; Nerve Tissue; Prostaglandins; Rabbits; Rats; Respiratory System
PubMed: 4803608
DOI: No ID Found -
Revue Medicale de Liege Jul 1974
Topics: Chemical Phenomena; Chemistry; Female; Humans; Lipid Metabolism; Male; Prostaglandins; Reproduction
PubMed: 4469936
DOI: No ID Found -
L'union Medicale Du Canada Feb 1980
Topics: Cardiovascular System; Chemical Phenomena; Chemistry; Digestive System; Female; Humans; Inflammation; Labor, Induced; Male; Metabolism; Nervous System; Pregnancy; Prostaglandin Antagonists; Prostaglandins; Reproduction; Respiratory System
PubMed: 7414759
DOI: No ID Found -
Biologie Medicale 1969
Topics: Animals; Humans; Prostaglandins
PubMed: 4251640
DOI: No ID Found -
Bioorganicheskaia Khimiia May 1983The quantitative study of the processes that accompany nonenzymatic degradation of prostaglandin H2 has been carried out. The thiobarbituric acid test which shows the...
The quantitative study of the processes that accompany nonenzymatic degradation of prostaglandin H2 has been carried out. The thiobarbituric acid test which shows the content of malonic dialdehyde in the reaction mixture has been used to study kinetics of the degradation. The apparent rate constants of this process have been pH-independent over the pH-range 5,5-9,5, and the calculated conversion halftime changes from 5,8 to 3,6 min at these pH values. Thromboxane synthetase from human platelets has been chosen to demonstrate the possibility of application of thiobarbituric acid test for determination of the activity of prostaglandin endoperoxide convertase. It has been shown that the apparent rate constant of the reaction in the presence of the enzyme is the linear function of its concentration.
Topics: Chemical Phenomena; Chemistry; Dinoprost; Dinoprostone; Humans; Intramolecular Oxidoreductases; Isomerases; Kinetics; Lipocalins; Malondialdehyde; Prostaglandin Endoperoxides; Prostaglandin Endoperoxides, Synthetic; Prostaglandin H2; Prostaglandin-E Synthases; Prostaglandin-Endoperoxide Synthases; Prostaglandins E; Prostaglandins F; Prostaglandins H; Thiobarbiturates
PubMed: 6435639
DOI: No ID Found -
Molecules (Basel, Switzerland) Jul 2022The 8-iso-prostaglandin F2α (8-iso-PGF2α) biomarker is used as the gold standard for tracing lipid oxidative stress in vivo. The analysis of urinary 8-iso-PGF2α is...
The 8-iso-prostaglandin F2α (8-iso-PGF2α) biomarker is used as the gold standard for tracing lipid oxidative stress in vivo. The analysis of urinary 8-iso-PGF2α is challenging when dealing with trace amounts of 8-iso-PGF2α and the complexity of urine matrixes. A packed-fiber solid-phase extraction (PFSPE)−coupled with HPLC-MS/MS−method, based on polystyrene (PS)-electrospun nanofibers, was developed for the specific determination of 8-iso-PGF2α in urine and compared with other newly developed LC-MS/MS methods. The method, which simultaneously processed 12 samples within 5 min on a self-made semi-automatic array solid-phase extraction processor, was the first to introduce PS-electrospun nanofibers as an adsorbent for the extraction of 8-iso-PGF2α and was successfully applied to real urine samples. After optimizing the PFSPE conditions, good linearity in the range of 0.05−5 ng/mL with R2 > 0.9996 and a satisfactory limit of detection of 0.015 ng/mL were obtained, with good intraday and interday precision (RSD < 10%) and recoveries of 95.3−103.8%. This feasible method is expected to be used for the batch quantitative analysis of urinary 8-iso-PGF2α.
Topics: Biomarkers; Chromatography, High Pressure Liquid; Chromatography, Liquid; Dinoprost; Oxidative Stress; Prostaglandins F; Solid Phase Extraction; Tandem Mass Spectrometry
PubMed: 35889290
DOI: 10.3390/molecules27144417 -
The Journal of Pharmacy and Pharmacology Feb 1976
Topics: Animals; Biodegradation, Environmental; Carbenoxolone; Gastric Mucosa; Guinea Pigs; Histamine N-Methyltransferase; Humans; Hydroxyprostaglandin Dehydrogenases; In Vitro Techniques; Indomethacin; Lung; Oxidation-Reduction; Prostaglandins; Prostaglandins F; Triterpenes
PubMed: 6674
DOI: 10.1111/j.2042-7158.1976.tb04113.x