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Medizinische Klinik Feb 1973
Review
Topics: Abortion, Induced; Adenylyl Cyclases; Animals; Autonomic Nervous System; Blood Pressure; Cardiovascular System; Cats; Central Nervous System; Chemical Phenomena; Chemistry; Contraceptives, Postcoital; Diuresis; Dogs; Enzyme Activation; Enzyme Repression; Female; Fertility; Gastric Mucosa; Genitalia, Female; Humans; Labor, Obstetric; Lipid Metabolism; Male; Menstruation; Muscle, Smooth; Platelet Adhesiveness; Pregnancy; Prostaglandin Antagonists; Prostaglandins; Rabbits; Semen
PubMed: 4633865
DOI: No ID Found -
Il Policlinico. Sezione Pratica Jun 1972
Review
Topics: Blood Pressure; Cell Membrane; Chemical Phenomena; Chemistry; Endometrium; Fatty Acids; Female; Glomerular Filtration Rate; Humans; Male; Placenta; Platelet Adhesiveness; Prostaglandins; Renin
PubMed: 4555756
DOI: No ID Found -
Les Cahiers de Medecine Jun 1972
Review
Topics: Adenylyl Cyclases; Adipose Tissue; Animals; Blood Coagulation; Blood Platelets; Blood Pressure; Calcium; Carbohydrate Metabolism; Cardiovascular System; Central Nervous System; Chemical Phenomena; Chemistry; Cyclic AMP; Digestive System; Fatty Acids, Nonesterified; Genitalia; Humans; Kidney; Peripheral Nerves; Prostaglandins; Respiratory System
PubMed: 4342566
DOI: No ID Found -
Prostaglandins Apr 1987The release of prostaglandin E2 and F2 alpha, thromboxane B2 and 6-keto-prostaglandin F1 alpha was measured in isolated human placental cotyledons perfused under high-...
The release of prostaglandin E2 and F2 alpha, thromboxane B2 and 6-keto-prostaglandin F1 alpha was measured in isolated human placental cotyledons perfused under high- and low-oxygen conditions. Also the effect of reoxygenation on prostaglandin production was studied. During the high-oxygen period, prostaglandin E2 accounted for 44% and 6-keto-prostaglandin F1 alpha for 28% of all prostaglandin release, and the rank order of prostaglandin release was E2 greater than 6-keto-prostaglandin F1 alpha greater than thromboxane B2 greater than prostaglandin F2 alpha. Hypoxia had no significant effect on quantitative prostaglandin release, but the ratio of prostaglandin E2 to prostaglandin F2 alpha was significantly increased. After the hypoxic period during reoxygenation the release of 6-keto-prostaglandin F1 alpha was significantly decreased, as was the ratio of 6-keto-prostaglandin F1 alpha to thromboxane B2. Also the ratio of the vasodilating prostaglandins (E2, 6-keto-prostaglandin F1 alpha) to the vasoconstricting prostaglandins (thromboxane B2, prostaglandin F2 alpha) was decreased during reoxygenation period. With the constant flow rate, the perfusion pressure increased during hypoxia in six and was unchanged in three preparations. The results indicate that changes in the tissue oxygenation in the placenta affect prostaglandin release in the fetal placental circulation. This may also have circulatory consequences.
Topics: Aerobiosis; Anaerobiosis; Female; Humans; In Vitro Techniques; Kinetics; Oxygen Consumption; Perfusion; Placenta; Prostaglandins
PubMed: 3602415
DOI: 10.1016/0090-6980(87)90278-4 -
Life Sciences Apr 1974
Comparative Study
Topics: Animals; Chromatography; Chromatography, Ion Exchange; Chromatography, Thin Layer; Kidney Cortex; Kidney Medulla; Organ Specificity; Prostaglandins; Rabbits; Silicon Dioxide; Tritium
PubMed: 4364280
DOI: 10.1016/0024-3205(74)90163-5 -
Archives of Biochemistry and Biophysics Apr 1979
Topics: Lipids; Oxidation-Reduction; Peroxides; Prostaglandin-Endoperoxide Synthases; Prostaglandins; Prostaglandins G
PubMed: 111619
DOI: 10.1016/0003-9861(79)90038-9 -
Klinische Wochenschrift Aug 1971
Review
Topics: Animals; Blood Circulation; Blood Platelets; Blood Vessels; Bronchi; Bronchodilator Agents; Carbohydrate Metabolism; Cats; Central Nervous System; Chemical Phenomena; Chemistry; Coronary Disease; Diet Therapy; Digestive System; Dilatation; Dogs; Female; Fertility; Heart; Humans; In Vitro Techniques; Linoleic Acids; Lipid Metabolism; Muscles; Oxytocics; Platelet Adhesiveness; Prostaglandins; Rats; Uterus
PubMed: 4937153
DOI: 10.1007/BF01485211 -
Biochimica Et Biophysica Acta Dec 1982The profiles of circulating metabolites of prostaglandin F2 alpha were investigated in a number of species, viz. rat, rabbit, guinea pig, cattle and sheep. The aim of... (Comparative Study)
Comparative Study
The profiles of circulating metabolites of prostaglandin F2 alpha were investigated in a number of species, viz. rat, rabbit, guinea pig, cattle and sheep. The aim of the study was to identify in each animal major plasma metabolites that outlast the initially formed, short-lived 15-keto-13, 14-dihydroprostaglandin F2 alpha and might thus serve as better parameters for monitoring prostaglandin production in vivo. Tritium-labeled prostaglandin F2 alpha was injected intravenously and frequent blood samples were collected. The metabolic profiles at different stages were visualized using two-dimensional thin-layer chromatography and autoradiography. Identification of circulating products was achieved by comparison with reference compounds using several chromatographic methods, and by gas chromatography-mass spectrometry in cases where larger amounts of the prostaglandin had been administered. In the rabbit a similar study was also done with tritium-labeled prostaglandin E2. Certain species differences were seen in the removal of labeled compounds from the circulation, the elimination being most efficient in the guinea pig. Further differences were seen in the profiles of circulating prostaglandin metabolites. The first appearing major prostaglandin F2 alpha metabolite was always 15-ketodihydroprostaglandin F2 alpha. However, this compound was later replaced in the circulation by a number of more degraded products, the profiles of which were relatively typical for each species. Thus, in cattle, rat and guinea pig, the earliest-formed metabolites, 15-ketodihydroprostaglandin F2 alpha and its tetranor counterpart, 5 alpha, 7 alpha-dihydroxy-11-ketotetranorprostanoic acid, remained comparatively prominent plasma products, whereas highly polar dicarboxylic acids rapidly dominated the metabolite spectrum in the ovine and lapine circulation. These differences were further supported by separate kinetic experiments, using unlabeled prostaglandin F2 alpha and radioimmunological determination of formed products. These latter experiments also demonstrated further pronounced species differences in the rat of elimination of the different prostaglandin metabolites. A considerable interconversion between prostaglandin E and F compounds was also demonstrated in some species. In conclusion, the traditional prostaglandin parameters in plasma, the 15-ketodihydrometabolites, were found not to be the best parameters in all species. It is suggested that species differences in prostaglandin metabolism are taken ito consideration when the optimal analytical protocol is sought for future biological studies. Some alternatives are suggested in the present paper.
Topics: Animals; Cattle; Dinoprost; Dinoprostone; Female; Kinetics; Prostaglandins; Prostaglandins E; Prostaglandins F; Rabbits; Rats; Rats, Inbred Strains; Sheep; Species Specificity; Tritium
PubMed: 6960930
DOI: 10.1016/0005-2760(82)90316-2 -
Seikagaku. the Journal of Japanese... Jul 1986
Review
Topics: Animals; Biological Transport; Cell Division; Humans; Muscle Contraction; Muscle Relaxation; Prostaglandins; Receptors, Prostaglandin
PubMed: 3537162
DOI: No ID Found -
Biochemical and Biophysical Research... May 1984We studied in rats the effect of dexamethasone (2.5 mg/kg per week) on the conversion of radiolabeled arachidonic acid to prostaglandins by renal medulla slices,...
We studied in rats the effect of dexamethasone (2.5 mg/kg per week) on the conversion of radiolabeled arachidonic acid to prostaglandins by renal medulla slices, microsomes, and homogenates. The steroid did not affect the rate of conversion of arachidonic acid to prostaglandins by renal medulla slices, but significantly increased the rate of conversion by both the microsomes and the 10,000 X g supernatant of renal medulla homogenates. We conclude (a) that dexamethasone treatment increases the activity of renal medulla prostaglandin synthetase measured in broken cells preparations, and (b) that such a change in enzyme activity is not manifested by augmentation of prostaglandin synthesis in renal medulla slices incubated with exogenous arachidonic acid.
Topics: Animals; Cytosol; Dexamethasone; Dinoprost; Dinoprostone; Enzyme Activation; Kidney Medulla; Male; Microsomes; Prostaglandin D2; Prostaglandin-Endoperoxide Synthases; Prostaglandins D; Prostaglandins E; Prostaglandins F; Rats; Rats, Inbred Strains
PubMed: 6428405
DOI: 10.1016/0006-291x(84)90715-0