-
Journal of Clinical Pathology Oct 1975A method of typing isolates of Proteus mirabilis and Proteus vulgaris is described based on the sensitivity of the organisms to bacteriocine. Twelve standard proteocine...
A method of typing isolates of Proteus mirabilis and Proteus vulgaris is described based on the sensitivity of the organisms to bacteriocine. Twelve standard proteocine producing strains were selected from a large number of isolates tested, and from these liquid proteocine preparations were prepared. The sensitivities of 1805 isolates to these 12 preparations were then determined and none was found to be untypable. From the results a bacteriocine typing system has been developed.
Topics: Bacteriocins; Microbial Sensitivity Tests; Proteus mirabilis; Proteus vulgaris
PubMed: 765360
DOI: 10.1136/jcp.28.10.784 -
Zentralblatt Fur Bakteriologie,... Nov 1972
Topics: Bacteriocins; Bacteriological Techniques; Methods; Proteus; Proteus mirabilis; Proteus vulgaris
PubMed: 4144798
DOI: No ID Found -
The Journal of Hospital Infection Sep 1985During 1972-1982, 318 isolates of Proteus spp. were recovered from blood cultures, accounting for 14.4% of the aerobic, Gram-negative isolates from blood in a large...
During 1972-1982, 318 isolates of Proteus spp. were recovered from blood cultures, accounting for 14.4% of the aerobic, Gram-negative isolates from blood in a large general hospital. Bacteraemia was most common among male patients on the internal medicine service. A high level of resistance to antibiotic agents was found. Since 1976, 49 proteus isolates were sensitive to either gentamicin or tobramycin, but not to both.
Topics: Anti-Bacterial Agents; Drug Resistance, Microbial; Hospitals, General; Humans; Proteus; Proteus Infections; Retrospective Studies; Sepsis
PubMed: 2865295
DOI: No ID Found -
Archives Roumaines de Pathologie... 1978
-
British Journal of Biomedical Science Jun 1994Two hundred clinical isolates of members of the genus Proteus were definitively identified and their antimicrobial susceptibilities to 12 antimicrobials tested, 176...
Two hundred clinical isolates of members of the genus Proteus were definitively identified and their antimicrobial susceptibilities to 12 antimicrobials tested, 176 isolates (88%) being identified as Proteus mirabilis, 12 strains (6%) as Proteus vulgaris and 12 strains (6%) as Proteus penneri. Most strains were isolated from pus (62.5%) and urine (34%), but in general there were no significant differences in the rates of isolation of any of the species by age or sex, although it was noted that P. vulgaris was only isolated from patients belonging to the older age group (> 5 years). The Proteus spp. were notably susceptible to nalidixic acid, ceftazidime and the aminoglycosides tested, and resistant to polymyxin B and colistin. The inclination of certain Proteus species to be susceptible or resistant to certain antimicrobials was noted, but strain differences also existed. The results of the study confirm the importance of performing antimicrobials susceptibility testing of each Proteus isolate to avoid potentially misleading therapy. The noted discrepancy in the result of the susceptibility of P. penneri to chloramphenicol as tested by different standard methods merits further investigation.
Topics: Anti-Bacterial Agents; Drug Resistance, Microbial; Female; Humans; In Vitro Techniques; Male; Microbial Sensitivity Tests; Proteus; Suppuration; Urine
PubMed: 8049615
DOI: No ID Found -
Enfermedades Infecciosas Y... 1991
Review
Topics: Animals; Bacterial Adhesion; Bacterial Proteins; Bacterial Typing Techniques; Bacteriological Techniques; Disease Vectors; Hemolysin Proteins; Humans; Mice; Phagocytes; Proteus; Proteus Infections; Proteus mirabilis; Reproduction; Urease; Virulence
PubMed: 1799614
DOI: No ID Found -
Enfermedades Infecciosas Y... Nov 1991
Review
Topics: Animals; Antibodies, Bacterial; Bacteremia; Diarrhea; Feces; Humans; Prevalence; Proteus; Proteus Infections; Respiratory Tract Infections; Urinary Tract Infections
PubMed: 1822710
DOI: No ID Found -
Enfermedades Infecciosas Y... Oct 1991
Review
Topics: Animals; Bacterial Adhesion; Bacterial Proteins; Bacterial Typing Techniques; Bacteriological Techniques; Cross Infection; Hemolysin Proteins; Humans; Mice; Proteus; Proteus Infections; Urease
PubMed: 1805954
DOI: No ID Found -
Zhonghua Yi Xue Za Zhi = Chinese... Jul 1999Proteus bacteria are a well-known cause of urinary tract infections (UTIs). The prevalence of UTIs is high among catheterized patients and those undergoing manipulation...
BACKGROUND
Proteus bacteria are a well-known cause of urinary tract infections (UTIs). The prevalence of UTIs is high among catheterized patients and those undergoing manipulation of the urinary tract. This study assessed the prevalence of UTIs due to Proteus species, the predisposing factors, complications and extent of antimicrobial resistance at a regional teaching hospital.
METHODS
Urine samples in sterile containers from inpatients and outpatients were inoculated onto cysteine lactose electrolyte deficient agar and sheep blood agar plates with calibrated (0.001 ml) platinum loops and incubated aerobically at 35 degrees C to 37 degrees C for 18 to 24 hours. A colony count of 10(5) bacteria/ml or more was the criterion for significant bacteriuria. Proteus spp were identified and classified into four groups. Susceptibility testing was performed via the Kirby-Bauer disc diffusion technique on Müeller-Hinton agar using ampicillin (10 micrograms), tetracycline (30 micrograms), nalidixic acid (30 micrograms), gentamicin (10 micrograms), nitrofurantoin (30 micrograms), co-trimoxazole (30 micrograms) and cefuroxime (30 micrograms).
RESULTS
Of 1,397 urine specimens from hospital and community patients, 414 had one or more species of bacteria isolated, of which 74 (17.9%) were Proteus spp. Hospital-acquired UTIs accounted for more than two-thirds (51/74, 68.9%) of Proteus spp isolates, while community-acquired UTIs accounted for approximately one-third (23/74, 31.1%) of all Proteus isolates. The prevalence of Proteus UTIs in males was 34 of 184 (18.5%) and was slightly higher than in females (40/230, 17.4%). P mirabilis was the most frequently isolated Proteus sp (55/74, 74.3%), followed by P vulgaris (9/74. 12.2%), Morganella morganii, (7/74, 9.5%) and Providencia rettgeri (3/74, 4.0%). Forty-nine of 55 (89%) P mirabilis isolates were biotype 2. Catheterization was the most common predisposing factor in 32.4% of hospital-acquired Proteus UTIs. More than 92% of Proteus isolates were sensitive to gentamicin and nalidixic acid, whereas, ampicillin (35%) and tetracycline (18%) were the least effective drugs.
CONCLUSIONS
Proteus was isolated from about 18% of patients with significant bacteriuria. Most isolates occurred in hospitalized patients with indwelling urinary catheters and in patients with benign prostatic hypertrophy, diabetes and prostatectomy. Proper catheter care may improve infection control and reduce the morbidity of UTIs associated with Proteus spp.
Topics: Adolescent; Adult; Aged; Aged, 80 and over; Child; Child, Preschool; Female; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Proteus; Urinary Tract Infections
PubMed: 10418178
DOI: No ID Found -
Zentralblatt Fur Bakteriologie,... Apr 1986All 33 investigated strains of Proteus penneri gave a green colour reaction with Kovacs' indole reagent after an incubation of about three days at 36 degrees C....
All 33 investigated strains of Proteus penneri gave a green colour reaction with Kovacs' indole reagent after an incubation of about three days at 36 degrees C. Furthermore, 26% of the 51 strains of Proteus mirabilis studied also showed the green colour reaction but somewhat more weakly. The initial compound of this reaction seems to be a product of tryptophan metabolism which is formed only under aerobic conditions.
Topics: Culture Media; Indicators and Reagents; Indoles; Proteus; Proteus mirabilis; Spectrophotometry; Tryptophan
PubMed: 3526760
DOI: 10.1016/s0176-6724(86)80036-0