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PloS One 2017Proteus species are well-known opportunistic pathogens frequently associated with skin wound and urinary tract infections in humans and animals. O antigen diversity is...
Proteus species are well-known opportunistic pathogens frequently associated with skin wound and urinary tract infections in humans and animals. O antigen diversity is important for bacteria to adapt to different hosts and environments, and has been used to identify serotypes of Proteus isolates. At present, 80 Proteus O-serotypes have been reported. Although the O antigen structures of most Proteus serotypes have been identified, the genetic features of these O antigens have not been well characterized. The O antigen gene clusters of Proteus species are located between the cpxA and secB genes. In this study, we identified 55 O antigen gene clusters of different Proteus serotypes. All clusters contain both the wzx and wzy genes and exhibit a high degree of heterogeneity. Potential functions of O antigen-related genes were proposed based on their similarity to genes in available databases. The O antigen gene clusters and structures were compared, and a number of glycosyltransferases were assigned to glycosidic linkages. In addition, an O serotype-specific suspension array was developed for detecting 31 Proteus serotypes frequently isolated from clinical specimens. To our knowledge, this is the first comprehensive report to describe the genetic features of Proteus O antigens and to develop a molecular technique to identify different Proteus serotypes.
Topics: Genes, Bacterial; O Antigens; Polymerase Chain Reaction; Proteus
PubMed: 28817637
DOI: 10.1371/journal.pone.0183267 -
Journal of Clinical Pathology Jan 1968The results of sensitivity test for four Proteus species were compared. P. mirabilis was, in general, more drug sensitive than the other species and P. rettgeri was the...
The results of sensitivity test for four Proteus species were compared. P. mirabilis was, in general, more drug sensitive than the other species and P. rettgeri was the least sensitive. The conclusion was drawn that species identification of Proteus is essential in routine bacteriological diagnosis.
Topics: Anti-Bacterial Agents; Culture Media; Drug Resistance, Microbial; Humans; Proteus; Species Specificity
PubMed: 5697323
DOI: 10.1136/jcp.21.1.103 -
Journal of Clinical Microbiology Oct 1999
Topics: Proteus
PubMed: 10515741
DOI: 10.1128/JCM.37.10.3435-3435.1999 -
Journal of General Microbiology Jun 1980R plasmids R702, R711b, R1, D, Rip69, R447b, R471 and R394, belonging to different incompatibility groups, mobilized the Proteus morganii 2815 chromosome. Matings...
R plasmids R702, R711b, R1, D, Rip69, R447b, R471 and R394, belonging to different incompatibility groups, mobilized the Proteus morganii 2815 chromosome. Matings employing plasmids R711b or R702 as sex factors with doubly auxotrophic recipients produced recombinants characterized by the obligatory inheritance of ser-1+, irrespective of the selected marker.
Topics: Chromosomes, Bacterial; Crosses, Genetic; Gene Frequency; Genetic Markers; Proteus; R Factors; Recombination, Genetic
PubMed: 6934261
DOI: 10.1099/00221287-118-2-539 -
Journal of Bioscience and Bioengineering Apr 2013The novel bioelectricity-generating bacterium of Proteus hauseri ZMd44 has been first identified to produce McoA-laccase (EC 1.10.3.2) induced by copper sulphate. The...
The novel bioelectricity-generating bacterium of Proteus hauseri ZMd44 has been first identified to produce McoA-laccase (EC 1.10.3.2) induced by copper sulphate. The optimal concentration of copper is 3 mM as supplementation at the beginning of culture or early exponential growth phase, during which laccase is predominantly synthesized. Moreover, the whole cellular and intracellular activities of laccase increase in the degrees of inducible copper concentrations. A possible mechanism for this phenomenon is that copper ions enhance the laccase genetic transcription level during the laccase synthesis thus granting this strain in copper tolerance. McoA-laccase belongs to typical type 1 (T1) Cu site laccase by electron paramagnetic resonance (EPR) analysis of intracellular enzyme. From our results, the optimal temperature and pH are 60°C and pH 2.2, respectively. The kinetic profiles show that this enzyme is stable under 50°C and in the slightly acidic environment, making it a potentially useful enzyme in dye decolorization, paper-pulp bleaching and bioremediation industries.
Topics: Cations; Copper; Copper Sulfate; Electron Spin Resonance Spectroscopy; Laccase; Proteus
PubMed: 23153927
DOI: 10.1016/j.jbiosc.2012.10.012 -
Journal of General Microbiology Nov 1962
Topics: Fermentation; Proteus; Proteus vulgaris; Sucrose
PubMed: 14021948
DOI: 10.1099/00221287-29-3-455 -
Archives of Dermatology Aug 1970
Topics: Chloramphenicol; Endarterectomy; Humans; Injections, Intravenous; Lincomycin; Male; Middle Aged; Nails; Pigmentation Disorders; Proteus; Proteus Infections; Skin Manifestations
PubMed: 5430310
DOI: No ID Found -
Journal of General Microbiology Oct 1960
Topics: Proteus; Proteus vulgaris
PubMed: 13694275
DOI: 10.1099/00221287-23-2-209 -
Antimicrobial Agents and Chemotherapy Jul 1977Wild-type Proteus strains are usually resistant to colistin. We have observed an increase in the level of colistin resistance in four clinical isolates of P. vulgaris,...
Wild-type Proteus strains are usually resistant to colistin. We have observed an increase in the level of colistin resistance in four clinical isolates of P. vulgaris, P. morganii, and P. rettgeri by prior treatment with subinhibitory concentrations of the drug. The enhanced resistance in these strains was lost after cultivating them in colistin-free medium, indicating that the enhanced resistance in the Proteus group is inducible.
Topics: Colistin; Culture Media; Depression, Chemical; Drug Resistance, Microbial; Proteus; Time Factors
PubMed: 883810
DOI: 10.1128/AAC.12.1.1 -
Applied Microbiology Mar 1972Thirteen indole-producing, swarming strains of Proteus were identified by additional biochemical testing as being Proteus mirabilis. These strains were characterized by...
Thirteen indole-producing, swarming strains of Proteus were identified by additional biochemical testing as being Proteus mirabilis. These strains were characterized by 40 biochemical tests and by susceptibility testing to 11 antibiotics. All produced ornithine decarboxylase and were susceptible to members of the penicillin-cephalosporin groups of antibiotics. These indole-positive strains are similar to indole-negative P. mirabilis and are distinctly different from P. vulgaris. For greatest accuracy and to insure greatest clinical relevancy, P. mirabilis and P. vulgaris should be distinguished from one another in the laboratory by performing both the indole and ornithine decarboxylase tests.
Topics: Ampicillin; Anti-Bacterial Agents; Carboxy-Lyases; Cephaloridine; Cephalothin; Indoles; Microbial Sensitivity Tests; Ornithine; Penicillins; Proteus; Proteus mirabilis; Proteus vulgaris
PubMed: 4553804
DOI: 10.1128/am.23.3.592-594.1972