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Letters in Applied Microbiology 2003To analyse the influence of cell growth rate and iron concentration on the production of pyoverdines (PVDs) and of their reduced dihydro forms by three fluorescent...
AIMS
To analyse the influence of cell growth rate and iron concentration on the production of pyoverdines (PVDs) and of their reduced dihydro forms by three fluorescent Pseudomonas strains (P. putida BTP16, P. fluorescens BTP7 and P. aeruginosa 7NSK2).
METHODS
PVD and dihydropyoverdine (DHPVD) productions were determined by LC ESI-MS and spectrophotometry during batch and chemostat culture at different dilution rates.
SIGNIFICANCE
The relatively high PVD-to-DHPVD ratio (0.57) observed in pH-controlled batch cultures suggested that a base-catalysed chemical oxidation of the dihydroform is not the prime mechanism involved in generating PVDs. Interestingly, in chemostat cultures the PVD-to-DHPVD ratio was significantly reduced at low specific growth rate. Our results suggest that the oxidation of DHPVD to PVD is catalysed by an iron-dependent enzymatic reaction rather than a chemical oxidation.
Topics: Biomass; Fermentation; Fluorescence; Oligopeptides; Pigments, Biological; Pseudomonas; Pseudomonas aeruginosa; Pseudomonas fluorescens; Pseudomonas putida
PubMed: 12680934
DOI: 10.1046/j.1472-765x.2003.01304.x -
Environmental Microbiology Reports Oct 2014Pseudomonads are a diverse and ecologically successful group of γ-proteobacteria present in many environments (terrestrial, freshwater and marine), either free living... (Review)
Review
Pseudomonads are a diverse and ecologically successful group of γ-proteobacteria present in many environments (terrestrial, freshwater and marine), either free living or associated with plants or animals. Their success is at least partly based on their ability to grow over a wide range of temperatures, their capacity to withstand different kinds of stress and their great metabolic versatility. Although the optimal growth temperature of pseudomonads is usually close to 25–30°C, many strains can also grow between 5°C and 10°C, and some of them even close to 0°C. Such low temperatures strongly affect the physicochemical properties of macromolecules, forcing cells to evolve traits that optimize growth and help them withstand cold-induced stresses such as increased levels of reactive oxygen species, reduced membrane fluidity and enzyme activity, cold-induced protein denaturation and the greater stability of DNA and RNA secondary structures. This review gathers the information available on the strategies used by pseudomonads to adapt to low temperature growth, and briefly describes some of the biotechnological applications that might benefit from cold-adapted bacterial strains and enzymes, e.g., biotransformation or bioremediation processes to be performed at low temperatures.
Topics: Adaptation, Physiological; Bacterial Proteins; Cold Temperature; Pseudomonas
PubMed: 25646532
DOI: 10.1111/1758-2229.12150 -
International Journal of Systematic and... Jul 2000The description of Pseudomonas straminae lizuka and Komagata 1963 was emended with data newly obtained. The spelling of the name of this taxon is also corrected as...
The description of Pseudomonas straminae lizuka and Komagata 1963 was emended with data newly obtained. The spelling of the name of this taxon is also corrected as Pseudomonas straminea. Strains that were previously named 'Pseudomonas ochracea' were identified as P. straminea.
Topics: Base Composition; DNA, Bacterial; Nucleic Acid Hybridization; Oryza; Pseudomonas
PubMed: 10939658
DOI: 10.1099/00207713-50-4-1513 -
Wei Sheng Wu Xue Bao = Acta... Aug 1997A Gram-negative rod bacteria isolated from the hydrocardia of a hydropericarditis patient was reported. Maincharteristics of the strain are respiratory metabolism,...
A Gram-negative rod bacteria isolated from the hydrocardia of a hydropericarditis patient was reported. Maincharteristics of the strain are respiratory metabolism, oxidse positive and motility by one polar flagella. Through systematic morphological, physiological and biochemical identification; calculation of G+C mol%, and examination with Biolog Automated Systems, the species of Pseudomonas vesicularis are identified. We pointed out that the strain can infect an immuno-suppressed individual. The change of taxonomic position of the species was discussed.
Topics: Female; Humans; Pericardial Effusion; Pseudomonas
PubMed: 9863204
DOI: No ID Found -
Environmental Microbiology Mar 2007OprD has been widely described for Pseudomonas aeruginosa at both structural and functional levels. Here, we describe the sequence diversity of the OprD proteins from...
OprD has been widely described for Pseudomonas aeruginosa at both structural and functional levels. Here, we describe the sequence diversity of the OprD proteins from other fluorescent Pseudomonads. We analysed the sequence of the oprD gene in each of the 49 Pseudomonas isolates, mostly putida and fluorescens species, obtained from various environmental sources, including soil, rhizosphere and hospitals. Phylogeny based on OprD sequences distinguished three well-separated clusters in the P. fluorescens species whereas P. putida isolates formed only one cluster. The OprD sequences were generally well conserved within each cluster whereas on the opposite, they were highly variable from one cluster to another and particularly with regards to the cluster of P. aeruginosa. Predicted secondary structures, based on the topological model elaborated for P. aeruginosa, suggest signatures in the large extracellular loops of OprD, which are linked to the OprD-based clusters. Correlations between these OprD-based clusters and ecological niches, growth on various carbon sources and antibiotic sensitivity were investigated.
Topics: Amino Acid Sequence; DNA, Bacterial; Environmental Microbiology; Genetic Variation; Hospitals; Molecular Sequence Data; Plant Roots; Porins; Pseudomonas; Pseudomonas aeruginosa; Sequence Analysis, DNA; Soil Microbiology
PubMed: 17298381
DOI: 10.1111/j.1462-2920.2006.01191.x -
Journal of Bacteriology Apr 1984A silver-resistant strain of Pseudomonas stutzeri was isolated from a silver mine. It harbored three plasmids, the largest of which (pKK1; molecular weight, 49.4 X...
A silver-resistant strain of Pseudomonas stutzeri was isolated from a silver mine. It harbored three plasmids, the largest of which (pKK1; molecular weight, 49.4 X 10(6)) specified silver resistance. Plasmid pKK1 was apparently nonconjugative but could be transferred to Pseudomonas putida by mobilization with plasmid R68.45.
Topics: Anti-Bacterial Agents; Conjugation, Genetic; Mining; Pseudomonas; R Factors; Silver
PubMed: 6715284
DOI: 10.1128/jb.158.1.389-392.1984 -
Applied Microbiology Jan 1973Strains of Pseudomonas producing fluorescin but no pyocyanin or pyorubrin were studied by biochemical and antibiotic sensitivity testing. A rapid nitrate test was found...
Strains of Pseudomonas producing fluorescin but no pyocyanin or pyorubrin were studied by biochemical and antibiotic sensitivity testing. A rapid nitrate test was found to be useful in distinguishing P. aeruginosa (positive) from P. fluorescens and P. putida (both negative). A shortened gelatin test differentiated P. fluorescens (positive) from P. putida (negative). P. fluorescens and P. putida were very sensitive to low levels of kanamycin and resistant to carbenicillin, a pattern just the opposite of that obtained with P. aeruginosa.
Topics: Anti-Bacterial Agents; Bacteriological Techniques; Carbenicillin; Gelatin; Kanamycin; Nitrates; Penicillin Resistance; Pigments, Biological; Pseudomonas; Pseudomonas fluorescens; Species Specificity
PubMed: 4631431
DOI: 10.1128/am.25.1.107-110.1973 -
Microbiology (Reading, England) May 1999The pathway of butane metabolism by butane-grown 'Pseudomonas butanovora' was determined to be butane --> 1-butanol --> butyraldehyde butyrate. Butane was initially...
The pathway of butane metabolism by butane-grown 'Pseudomonas butanovora' was determined to be butane --> 1-butanol --> butyraldehyde butyrate. Butane was initially oxidized at the terminal carbon to produce 1-butanol. Up to 90% of the butane consumed was accounted for as 1-butanol when cells were incubated in the presence of 5 mM 1-propanol (to block subsequent metabolism of 1-butanol). No production of the subterminal oxidation product, 2-butanol, was detected, even in the presence of 5 mM 2-pentanol (an effective inhibitor of 2-butanol consumption). Ethane, propane and pentane, but not methane, were also oxidized. Butane-grown cells consumed 1-butanol and other terminal alcohols. Secondary alcohols, including 2-butanol, were oxidized to the corresponding ketones. Butyraldehyde was further oxidized to butyrate as demonstrated by blocking butyrate metabolism with 1 mM sodium valerate. Butyrate also accumulated from butane when cells were incubated with 1 mM sodium valerate. The pathway intermediates (butane, 1-butanol, butyraldehyde and butyrate) and 2-butanol stimulated O2 consumption by butane-grown cells. 1-Butanol, butyraldehyde and butyrate supported growth of 'P. butanovora', as did 2-butanol and lactate.
Topics: Alcohols; Alkanes; Biodegradation, Environmental; Butanes; Culture Media; Oxidation-Reduction; Oxygen Consumption; Pseudomonas
PubMed: 10376833
DOI: 10.1099/13500872-145-5-1173 -
Plant Disease Sep 2018Olive knot, caused by Pseudomonas savastanoi pv. savastanoi, is a limiting disease in the production of table and oil olives in California. The genetic variability among...
Olive knot, caused by Pseudomonas savastanoi pv. savastanoi, is a limiting disease in the production of table and oil olives in California. The genetic variability among 152 strains from major production areas of California was determined using BOX, ERIC, and REP primers in repetitive element sequence-based polymerase chain reaction. Overall genetic variability was low, and strains shared at least 82% similarity. Phenetic analyses identified several genotypes but most strains belonged to one of two major groups. Three copper-resistant strains had two fingerprints that were distinct from any of the sensitive strains, indicating that they may have been introduced from other production areas or hosts. In inoculations, two copper-resistant strains were mostly equally as virulent as two copper-sensitive strains. Inoculum was exuded at high levels (>10 CFU/g of knot tissue) within 10 min from hydrated olive knots, and concentrations were 2- to 3-log higher than the minimum needed to induce knot formation. Arbequina olive was significantly more susceptible to infection and developed a higher incidence of knots on leaf scar and lateral wounds (59.7 to 80.6% incidence) than Manzanillo (47.4 to 68.2% incidence). In wound-healing studies, both types of wounds were less susceptible to infection ≥10 days after injury, indicating a critical period for infection and application of bactericides during favorable environments.
Topics: California; Copper; Genetic Variation; Olea; Plant Diseases; Pseudomonas; Virulence
PubMed: 30125179
DOI: 10.1094/PDIS-11-17-1709-RE -
Biodegradation Apr 2010A novel bacterium capable of utilizing 2-sec-butylphenol as the sole carbon and energy source, Pseudomonas sp. strain MS-1, was isolated from freshwater sediment. Within...
A novel bacterium capable of utilizing 2-sec-butylphenol as the sole carbon and energy source, Pseudomonas sp. strain MS-1, was isolated from freshwater sediment. Within 30 h, strain MS-1 completely degraded 1.5 mM 2-sec-butylphenol in basal salt medium, with concomitant cell growth. A pathway for the metabolism of 2-sec-butylphenol by strain MS-1 was proposed on the basis of the identification of 3 internal metabolites-3-sec-butylcatechol, 2-hydroxy-6-oxo-7-methylnona-2,4-dienoic acid, and 2-methylbutyric acid-by gas chromatography-mass spectrometry analysis. Strain MS-1 degraded 2-sec-butylphenol through 3-sec-butylcatechol along a meta-cleavage pathway. Degradation experiments with various alkylphenols showed that the degradability of alkylphenols by strain MS-1 depended strongly on the position (ortho >> meta = para) of the alkyl substitute, and that strain MS-1 could degrade 2-alkylphenols with various sized and branched alkyl chain (o-cresol, 2-ethylphenol, 2-n-propylphenol, 2-isopropylphenol, 2-sec-butylphenol, and 2-tert-butylphenol), as well as a dialkylphenol (namely, 6-tert-butyl-m-cresol).
Topics: Biodegradation, Environmental; Geologic Sediments; Molecular Sequence Data; Phenols; Phylogeny; Pseudomonas
PubMed: 19705287
DOI: 10.1007/s10532-009-9290-y