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Applied and Environmental Microbiology May 1993Two Pseudomonas isolates, named ES-1 and ES-2, were shown to possess a wide degradative spectrum for haloalkanes in general and bromoalkanes in particular but did not...
Two Pseudomonas isolates, named ES-1 and ES-2, were shown to possess a wide degradative spectrum for haloalkanes in general and bromoalkanes in particular but did not degrade nonsubstituted alkanes. The utilization of water-insoluble haloalkanes, such as 1-bromooctane, appeared to consist of three phases: (i) extracellular emulsification by a constitutively excreted, broad-spectrum surface-active agent, (ii) dehalogenation by an inducible hydrolytic dehalogenase (possibly periplasmic), and (iii) intracellular degradation of the residual carbon skeleton. Several observations suggest the existence of more than one dehalogenase in strain ES-2.
Topics: Alkanes; Biodegradation, Environmental; Excipients; Hydrocarbons, Brominated; Pseudomonas
PubMed: 8517736
DOI: 10.1128/aem.59.5.1403-1409.1993 -
Research in Microbiology 1991The study of phenotypic properties of 108 strains of Pseudomonas syringae pv. syringae van Hall isolated from Cherry laurel (50 strains) and various host plants (58... (Review)
Review
The study of phenotypic properties of 108 strains of Pseudomonas syringae pv. syringae van Hall isolated from Cherry laurel (50 strains) and various host plants (58 strains) and 53 strains of other pathovars of P. syringae and fluorescent Pseudomonas showed that the majority of the strains (91/108) were clustered in one phenon (phenon 14) containing strains commonly considered as P.s. pv. syringae. The present type strain of P.s. pv. syringae was distantly related to phenon 14. Other pathovars of P. syringae constituted 13 discrete phenons.
Topics: In Vitro Techniques; Phenotype; Plants; Pseudomonas; Pseudomonas Infections
PubMed: 1805313
DOI: 10.1016/0923-2508(91)90010-8 -
Microbiology and Molecular Biology... Sep 2000The extremely large number of leaves produced by terrestrial and aquatic plants provide habitats for colonization by a diversity of microorganisms. This review focuses... (Review)
Review
The extremely large number of leaves produced by terrestrial and aquatic plants provide habitats for colonization by a diversity of microorganisms. This review focuses on the bacterial component of leaf microbial communities, with emphasis on Pseudomonas syringae-a species that participates in leaf ecosystems as a pathogen, ice nucleus, and epiphyte. Among the diversity of bacteria that colonize leaves, none has received wider attention than P. syringae, as it gained notoriety for being the first recombinant organism (Ice(-) P. syringae) to be deliberately introduced into the environment. We focus on P. syringae to illustrate the attractiveness and somewhat unique opportunities provided by leaf ecosystems for addressing fundamental questions of microbial population dynamics and mechanisms of plant-bacterium interactions. Leaf ecosystems are dynamic and ephemeral. The physical environment surrounding phyllosphere microbes changes continuously with daily cycles in temperature, radiation, relative humidity, wind velocity, and leaf wetness. Slightly longer-term changes occur as weather systems pass. Seasonal climatic changes impose still a longer cycle. The physical and physiological characteristics of leaves change as they expand, mature, and senesce and as host phenology changes. Many of these factors influence the development of populations of P. syringae upon populations of leaves. P. syringae was first studied for its ability to cause disease on plants. However, disease causation is but one aspect of its life strategy. The bacterium can be found in association with healthy leaves, growing and surviving for many generations on the surfaces of leaves as an epiphyte. A number of genes and traits have been identified that contribute to the fitness of P. syringae in the phyllosphere. While still in their infancy, such research efforts demonstrate that the P. syringae-leaf ecosystem is a particularly attractive system with which to bridge the gap between what is known about the molecular biology of genes linked to pathogenicity and the ecology and epidemiology of associated diseases as they occur in natural settings, the field.
Topics: Bacteria; Bacterial Physiological Phenomena; Ecosystem; Ice; Plant Leaves; Plants; Pseudomonas
PubMed: 10974129
DOI: 10.1128/MMBR.64.3.624-653.2000 -
American Journal of Clinical Pathology Jan 1969
Topics: Abscess; Adult; Aged; Anti-Bacterial Agents; Culture Media; Drug Resistance, Microbial; Female; Humans; Male; Middle Aged; Pseudomonas; Pseudomonas Infections; Surgical Wound Infection; Urinary Tract Infections; Wounds, Gunshot
PubMed: 5766111
DOI: 10.1093/ajcp/51.1.58 -
Journal of Medical Microbiology Nov 1968
Topics: Adult; Aged; Culture Media; Female; Flagella; Humans; Leg Ulcer; Lymphadenitis; Male; Nitrates; Oxygen; Pseudomonas; Sepsis
PubMed: 5747915
DOI: 10.1099/00222615-1-2-195 -
Microbes and Environments 2010Over the past few decades, degradative plasmids have been isolated from bacteria capable of degrading a variety of both natural and man-made compounds. Degradative... (Review)
Review
Over the past few decades, degradative plasmids have been isolated from bacteria capable of degrading a variety of both natural and man-made compounds. Degradative plasmids belonging to three incompatibility (Inc) groups in Pseudomonas (IncP-1, P-7, and P-9) have been well studied in terms of their replication, maintenance, and capacity for conjugative transfer. The host ranges of these plasmids are determined by replication or conjugative transfer systems. The host range of IncP-1 is broad, that of IncP-9 is intermediate, and that of IncP-7 is narrow. To understand the behavior of these plasmids and their hosts in various environments, the survivability of inocula, stability or transferability, and efficiency of biodegradation in environments and microcosms have been monitored. The biodegradation and plasmid transfer in various environments have been observed for all three groups, although the kinds of transconjugants differed with the Inc groups. In some cases, the deletion and amplification of catabolic genes acted to reduce the production of toxic catabolic intermediates, or to increase the activity on a particular catabolic pathway. The combination of degradative genes, the plasmid backbone of each Inc group, and the host of the plasmids is key to the degraders adapting to various hosts or to heterogeneous environments.
Topics: Biodegradation, Environmental; Environmental Microbiology; Environmental Pollutants; Host Specificity; Plasmids; Pseudomonas
PubMed: 21576880
DOI: 10.1264/jsme2.me10155 -
International Journal of Systematic and... Mar 2005Two Gram-negative, non-fermentative, non-denitrifying, non-pigmented, rod-shaped bacteria that were motile by means of polar flagella, designated strains KMM 330(T) and...
Two Gram-negative, non-fermentative, non-denitrifying, non-pigmented, rod-shaped bacteria that were motile by means of polar flagella, designated strains KMM 330(T) and KMM 331, were isolated from a deep-sea sponge specimen and subjected to a polyphasic taxonomic study. The new isolates exhibited 16S rRNA gene sequence similarity of 99.9 %, and their mean level of DNA-DNA relatedness was 82 %. Phylogenetic analysis based on their 16S rRNA gene sequences placed the strains within the genus Pseudomonas as an independent deep clade. Strain KMM 330(T) shared highest sequence similarity (96.3 %) with each of Pseudomonas fulva NRIC 0180(T), Pseudomonas parafulva AJ 2129(T) and Pseudomonas luteola IAM 13000(T); sequence similarity to other recognized species of the genus Pseudomonas was below 95.7 %. The marine sponge isolates KMM 330(T) and KMM 331 could be distinguished from the other recognized Pseudomonas species based on a unique combination of their phenotypic characteristics, including growth in 8 or 10 % NaCl, the absence of pigments, the inability to denitrify and lack of carbohydrate utilization. On the basis of phylogenetic analysis, physiological and biochemical characterization, strains KMM 330(T) and KMM 331 should be classified as a novel species of the genus Pseudomonas, for which the name Pseudomonas pachastrellae sp. nov. is proposed. The type strain is KMM 330(T) (=JCM 12285(T)=NRIC 0583(T)=CCUG 46540(T)).
Topics: Animals; Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; Genes, rRNA; Molecular Sequence Data; Phenotype; Phylogeny; Porifera; Pseudomonas; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Species Specificity
PubMed: 15774686
DOI: 10.1099/ijs.0.63176-0 -
FEMS Microbiology Letters Jul 2014
Topics: Adaptation, Biological; Genome, Bacterial; Genomic Structural Variation; Pseudomonas
PubMed: 25060810
DOI: 10.1111/1574-6968.12517 -
International Journal of Systematic and... Sep 2010A novel Gram-negative, rod-shaped, motile, non-spore-forming bacterial strain, CMS(T), isolated from soil was characterized using phenotypic and molecular taxonomic...
A novel Gram-negative, rod-shaped, motile, non-spore-forming bacterial strain, CMS(T), isolated from soil was characterized using phenotypic and molecular taxonomic methods. 16S rRNA gene sequence analysis revealed that the organism belongs phylogenetically to the genus Pseudomonas. Pseudomonas monteilii, P. plecoglossicida and P. mosselii were the most closely related species, with 16S rRNA gene sequence similarities to the respective type strains of 99.79, 99.73 and 99.59 %. Relatively low gyrB gene sequence similarities (<90 %) and DNA-DNA reassociation values (<51 %) were obtained between the strain and its phylogenetically closest neighbours. The G+C content of strain CMS(T) was 62.7 mol%. The major cellular fatty acids were C(18 : 1) ω 7c, summed feature 3 (C(16 : 1) ω 7c and/or iso-C(15 : 0) 2-OH), C(16 : 0) and C(10 : 0) 3-OH. Based on the phenotypic and genetic evidence, the strain is suggested to represent a novel species, for which the name Pseudomonas taiwanensis sp. nov. is proposed. The type strain is CMS(T) (=BCRC 17751(T) =DSM 21245(T)).
Topics: DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Molecular Sequence Data; Phylogeny; Pseudomonas; RNA, Ribosomal, 16S; Soil Microbiology
PubMed: 19854877
DOI: 10.1099/ijs.0.014779-0 -
International Journal of Systematic and... Mar 2008An aerobic, Gram-negative, motile, non-pigmented bacterium, strain KMM 3042(T), isolated from a deep-sea brittle star in the Fiji Sea, was subjected to a polyphasic...
An aerobic, Gram-negative, motile, non-pigmented bacterium, strain KMM 3042(T), isolated from a deep-sea brittle star in the Fiji Sea, was subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed marine Mn(II)-oxidizing isolate S185-2B as the closest neighbour of strain KMM 3042(T) (99.9 % sequence similarity). The two strains formed a distinct lineage within the genus Pseudomonas adjacent to the members of the Pseudomonas borbori cluster, sharing highest sequence similarity of 97.4 and 97.0 %, respectively, with P. borbori DSM 17834(T) and Pseudomonas flavescens DSM 12071(T). The DNA-DNA hybridization value (71 %) between strains KMM 3042(T) and S185-2B confirmed their assignment to the same species. On the basis of phylogenetic analysis, DNA-DNA hybridization and physiological and biochemical characterization, strains KMM 3042(T) and S185-2B should be assigned to a novel species of the genus Pseudomonas, for which the name Pseudomonas marincola sp. nov. is proposed. The type strain is KMM 3042(T) (=NRIC 0729(T) =JCM 14761(T)).
Topics: Animals; Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; Echinodermata; Genes, rRNA; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; Pseudomonas; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Species Specificity
PubMed: 18319482
DOI: 10.1099/ijs.0.65406-0