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Applied and Environmental Microbiology Oct 1988Over 100 strains that utilized naphthalene as the only carbon and energy source were isolated from samples of marine sediments taken from a heavily polluted area. The...
Over 100 strains that utilized naphthalene as the only carbon and energy source were isolated from samples of marine sediments taken from a heavily polluted area. The isolates were characterized taxonomically and physiologically. Most of these strains belonged to the genus Pseudomonas, and seven of them did not fit any previous taxonomic description. They differed from type strains in a few biochemical characteristics and in the utilization of aromatic compounds. None had catechol 1,2-dioxygenase activity, and catechol 2,3-dioxygenase was responsible for the aromatic ring cleavage. DNA hybridization demonstrated a close relationship between two isolates and the Pseudomonas stutzeri type strain, and between five isolates and the Pseudomonas testosteroni type strain. On the basis of nutritional and enzymatic characteristics, it was assumed that the seven isolates represent new biovars belonging to the species P. testosteroni and P. stutzeri that are able to degrade aromatic hydrocarbons.
Topics: Biodegradation, Environmental; DNA, Bacterial; Flagella; Naphthalenes; Pseudomonas; Seawater; Water Microbiology
PubMed: 3202629
DOI: 10.1128/aem.54.10.2478-2485.1988 -
Environmental Microbiology Jun 2000
Topics: Biodegradation, Environmental; Biotransformation; Environmental Microbiology; Genome, Bacterial; Pseudomonas
PubMed: 11200438
DOI: 10.1046/j.1462-2920.2000.00126.x -
Scientific Reports Mar 2020Malachite green is a common environmental pollutant that poses a great threat to non-target organisms, including humans. This study reports the characterization of a...
Malachite green is a common environmental pollutant that poses a great threat to non-target organisms, including humans. This study reports the characterization of a bacterial strain, Pseudomonas veronii JW3-6, which was isolated from a malachite green enrichment culture. This strain degraded malachite green efficiently in a wide range of temperature and pH levels. Under optimal degradation conditions (32.4 °C, pH 7.1, and inoculum amount of 2.5 × 10 cfu/mL), P. veronii JW3-6 could degrade 93.5% of 50 mg/L malachite green within seven days. Five intermediate products from the degradation of malachite green were identified: leucomalachite green, 4-(dimethylamino) benzophenone, 4-dimethylaminophenol, benzaldehyde, and hydroquinone. We propose a possible degradation pathway based on these findings. The present study is the first to report the degradation of malachite green by P. veronii and the identification of hydroquinone as a metabolite in the degradation pathway.
Topics: Biodegradation, Environmental; Biodiversity; Environmental Microbiology; Kinetics; Metabolic Networks and Pathways; Molecular Structure; Phylogeny; Pseudomonas; RNA, Ribosomal, 16S; Rosaniline Dyes
PubMed: 32161360
DOI: 10.1038/s41598-020-61442-z -
Biotechnology Letters Feb 2020Developing a counterselective system for efficient markerless gene deletions in biocontrol strain P. protegens Pf-5.
OBJECTIVES
Developing a counterselective system for efficient markerless gene deletions in biocontrol strain P. protegens Pf-5.
RESULTS
We successfully implemented a markerless deletion of upp in Pf-5 to obtain the 5-FU resistant strain Pf5139. With this strain, we performed markerless gene deletions for each component of Gac/Rsm system and a 17 kb DNA fragment with the deletion ratio of 20 to 50%, and efficiently constructed a strain with triple deletions based on the suicide plasmid pJQ200UPP. In addition, there is no obvious connection between the deleted fragment length and the deletion ratio.
CONCLUSION
The upp-based counterselective system in this study is efficient and valuable for markerless gene deletions in Pf-5, indicating that it has great potential in the study of gene function and in the application of genome reduction for Pseudomonas strains.
Topics: Drug Resistance, Bacterial; Fluorouracil; Gene Deletion; Genes, Bacterial; Genetic Techniques; Pseudomonas
PubMed: 31781926
DOI: 10.1007/s10529-019-02772-5 -
American Journal of Clinical Pathology May 1971
Topics: Actinobacillus; Agglutination Tests; Anti-Bacterial Agents; Bacteriological Techniques; Culture Media; Fluorescent Antibody Technique; Hemagglutination Tests; Immune Sera; Melioidosis; Microbial Sensitivity Tests; Pseudomonas
PubMed: 4933030
DOI: 10.1093/ajcp/55.5.596 -
Canadian Journal of Microbiology Aug 1969
Topics: Cell Division; DNA, Bacterial; Densitometry; Microbial Sensitivity Tests; Microscopy, Electron; Pseudomonas
PubMed: 5344737
DOI: 10.1139/m69-152 -
Sensors (Basel, Switzerland) Jun 2014Quorum sensing (QS) is a bacterial cell-to-cell communication system controlling QS-mediated genes which is synchronized with the population density. The regulation of...
Quorum sensing (QS) is a bacterial cell-to-cell communication system controlling QS-mediated genes which is synchronized with the population density. The regulation of specific gene activity is dependent on the signaling molecules produced, namely N-acyl homoserine lactones (AHLs). We report here the identification and characterization of AHLs produced by bacterial strain ND07 isolated from a Malaysian fresh water sample. Molecular identification showed that strain ND07 is clustered closely to Pseudomonas cremoricolorata. Spent culture supernatant extract of P. cremoricolorata strain ND07 activated the AHL biosensor Chromobacterium violaceum CV026. Using high resolution triple quadrupole liquid chromatography-mass spectrometry, it was confirmed that P. cremoricolorata strain ND07 produced N-octanoyl-L-homoserine lactone (C8-HSL) and N-decanoyl-L-homoserine lactone (C10-HSL). To the best of our knowledge, this is the first documentation on the production of C10-HSL in P. cremoricolorata strain ND07.
Topics: Acyl-Butyrolactones; Cell Communication; Pseudomonas; Quorum Sensing; Species Specificity
PubMed: 24984061
DOI: 10.3390/s140711595 -
Applied Microbiology Nov 1972A total of 109 cultures of Pseudomonas putrefaciens isolated from clinical specimens were studied. The cultures were separated into two groups. The majority of the group...
A total of 109 cultures of Pseudomonas putrefaciens isolated from clinical specimens were studied. The cultures were separated into two groups. The majority of the group 1 isolates, comprising 31 cultures, were characterized by (i) growth in plain nutrient broth, but no growth in broth supplemented with NaCl at concentrations of 7% and above, (ii) no growth on Salmonella-Shigella (SS) agar, and (iii) production of acid from the carbohydrates, sucrose, maltose, arabinose, and dextrin. Most group 2 isolates, comprising 78 cultures, were (i) unable to grow in plain nutrient broth, but grew well in broth supplemented with NaCl at a concentration of 7 to 10%, (ii) able to grow on SS agar, and (iii) unable to produce detectable amounts of acid from any of the carbohydrates tested except for variable results with glucose and fructose.
Topics: Agar; Bacteriological Techniques; Carbohydrate Metabolism; Culture Media; Fructose; Glucose; Pseudomonas; Sodium Chloride
PubMed: 4565638
DOI: 10.1128/am.24.5.798-800.1972 -
The Journal of Applied Bacteriology Sep 1970
Topics: Bacteriological Techniques; Bacteriophage Typing; Culture Media; Plant Diseases; Plants; Pseudomonas; Species Specificity
PubMed: 4923557
DOI: 10.1111/j.1365-2672.1970.tb02225.x -
International Journal of Systematic and... Aug 2006Sixteen Gram-negative, rod-shaped, non-spore-forming isolates were obtained from a nitrifying inoculum. Analysis of repetitive sequence-based PCR and SDS-PAGE banding...
Sixteen Gram-negative, rod-shaped, non-spore-forming isolates were obtained from a nitrifying inoculum. Analysis of repetitive sequence-based PCR and SDS-PAGE banding patterns, 16S rRNA gene sequence analysis and DNA-DNA hybridizations showed that the isolates belonged to various groups within the genus Pseudomonas. One group of isolates could be assigned to Pseudomonas migulae and a second to Pseudomonas veronii. Two groups could be differentiated genotypically from each other and from all other currently known Pseudomonas species. Analysis of the fatty acid composition and physiological and biochemical tests allowed differentiation of these groups from their closest phylogenetic neighbours and they therefore represent two novel species within the genus Pseudomonas, for which the names Pseudomonas peli sp. nov. and Pseudomonas borbori sp. nov. are proposed, with strains LMG 23201(T) (=DSM 17833(T)=R-20805(T)) and LMG 23199(T) (=DSM 17834(T)=R-20821(T)), respectively, as the type strains.
Topics: Aquaculture; DNA, Bacterial; Fatty Acids; Molecular Sequence Data; Nitrites; Nucleic Acid Hybridization; Phylogeny; Pseudomonas; RNA, Bacterial; RNA, Ribosomal, 16S; Species Specificity; Water Microbiology
PubMed: 16902024
DOI: 10.1099/ijs.0.64224-0