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BMC Infectious Diseases Dec 2023Pseudomonas otitidis belongs to the genus Pseudomonas and causes various infections, including ear, skin, and soft tissue infections. P. otitidis has a unique... (Review)
Review
BACKGROUND
Pseudomonas otitidis belongs to the genus Pseudomonas and causes various infections, including ear, skin, and soft tissue infections. P. otitidis has a unique susceptibility profile, being susceptible to penicillins and cephalosporins but resistant to carbapenems, due to the production of the metallo-β-lactamase called POM-1. This revealed genetic similarities with Pseudomonas aeruginosa, which can sometimes lead to misidentification.
CASE PRESENTATION
We report the case of a 70-year-old Japanese male who developed cellulitis and bacteremia during chemotherapy for multiple myeloma. He was initially treated with meropenem, but blood culture later revealed gram-negative bacilli identified as P. otitidis using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Carbapenem resistance was predicted from previous reports; therefore, we switched to dual therapy with levofloxacin and cefepime, and favorable treatment results were obtained.
CONCLUSION
This is the first reported case of P. otitidis cellulitis and bacteremia in an immunocompromised patient. Carbapenems are typically used in immunocompromised patients and P. otitidis is often resistant to it. However, its biochemical properties are similar to those of Pseudomonas aeruginosa; therefore, its accurate identification is critical. In the present study, we rapidly identified P. otitidis using MALDI-TOF MS and switched from carbapenems to an appropriate antimicrobial therapy, resulting in a successful outcome.
Topics: Humans; Male; Aged; Anti-Bacterial Agents; Cellulitis; Pseudomonas; Carbapenems; Pseudomonas Infections; Pseudomonas aeruginosa; Bacteremia; Immunocompromised Host; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 38110897
DOI: 10.1186/s12879-023-08919-0 -
BMC Infectious Diseases Aug 2021Pseudomonas otitidis is a novel species of Pseudomonas bacteria that has been isolated from patients with otic infections. (Review)
Review
BACKGROUND
Pseudomonas otitidis is a novel species of Pseudomonas bacteria that has been isolated from patients with otic infections.
CASE PRESENTATION
In this report, we describe a case of a 59-year-old known with moderate chronic obstructive pulmonary disease with bronchiectasis and recurrent pneumonia where blood cultures revealed the growth of P. otitidis.
CONCLUSIONS
This case describes the first report of bacteraemia to P. otitidis and raises questions regarding the misdiagnosis and underestimation of the incidence of infections caused by this novel pathogen.
Topics: Bacteremia; Humans; Middle Aged; Pneumonia; Pseudomonas; Pulmonary Disease, Chronic Obstructive
PubMed: 34433417
DOI: 10.1186/s12879-021-06569-8 -
International Journal of Systematic and... Apr 2006A novel Pseudomonas species, for which the name Pseudomonas otitidis sp. nov. is proposed, was identified from clinical specimens of infected human ears. Forty-one...
A novel Pseudomonas species, for which the name Pseudomonas otitidis sp. nov. is proposed, was identified from clinical specimens of infected human ears. Forty-one pseudomonads (34 from patients with acute otitis externa, six from patients with acute otitis media with otorrhoea and one from a patient with chronic suppurative otitis media) were recovered that did not match any known species. On the basis of genetic analyses and biochemical characterization, these isolates were shown to belong to the genus Pseudomonas. 16S rRNA gene sequence analysis and DNA-DNA hybridization studies indicated that this novel bacterium is closely related to, but different from, Pseudomonas aeruginosa. A description of this species is based on polyphasic studies of 11 clinical isolates. The type strain of Pseudomonas otitidis is MCC10330T (=ATCC BAA-1130T = DSM 17224T).
Topics: DNA, Bacterial; Humans; Molecular Sequence Data; Otitis Media; Pseudomonas; RNA, Ribosomal, 16S
PubMed: 16585681
DOI: 10.1099/ijs.0.63753-0 -
Antimicrobial Agents and Chemotherapy Jan 2011Susceptibility to several β-lactams and β-lactamase production was investigated in a collection of 20 strains of Pseudomonas otitidis, a new Pseudomonas species that...
Susceptibility to several β-lactams and β-lactamase production was investigated in a collection of 20 strains of Pseudomonas otitidis, a new Pseudomonas species that has been recently recognized in association with otic infections in humans. All strains appeared to be susceptible to piperacillin, cefotaxime, ceftazidime, and aztreonam, while resistance or decreased susceptibility to carbapenems was occasionally observed. All strains were found to express metallo-β-lactamase (MBL) activity and to carry a new subclass B3 MBL gene, named bla(POM), that appeared to be highly conserved in this species. P. otitidis, therefore, is the first example of a pathogenic Pseudomonas species endowed with a resident MBL. The POM-1 protein from P. otitidis type strain MCC10330 exhibits the closest similarity (60 to 64%) to the L1 MBL of Stenotrophomonas maltophilia. Expression in Escherichia coli and Pseudomonas aeruginosa revealed that, similar to L1 and other subclass B3 MBLs, POM-1 confers decreased susceptibility or resistance to carbapenems, penicillins, and cephalosporins but not to aztreonam. Expression of the POM MBL in P. otitidis is apparently constitutive and, in most strains, does not confer a carbapenem-resistant phenotype. However, a strong inoculum size effect was observed for carbapenem MICs, and carbapenem-resistant mutants could be readily selected upon exposure to imipenem, suggesting that carbapenem-based regimens should be considered with caution for P. otitidis infections.
Topics: Amino Acid Sequence; Anti-Bacterial Agents; Bacterial Proteins; Carbapenems; Drug Resistance, Bacterial; Microbial Sensitivity Tests; Molecular Sequence Data; Phylogeny; Pseudomonas; Sequence Homology, Amino Acid; beta-Lactamases
PubMed: 21060106
DOI: 10.1128/AAC.01062-10 -
Letters in Applied Microbiology Jun 2024This study explores the ecofriendly synthesis of silver nanoparticles (AgNPs) using soil bacteria, Pseudomonas otitidis. The bio-synthesized AgNPs were characterized...
This study explores the ecofriendly synthesis of silver nanoparticles (AgNPs) using soil bacteria, Pseudomonas otitidis. The bio-synthesized AgNPs were characterized using various techniques, including UV-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-Ray Diffraction (XRD). UV-Visible spectroscopy revealed a distinct broad absorption band in the range of 443 nm, indicating the reduction of silver nitrate to AgNPs. XRD analysis provided evidence of the crystalline nature of the particles, with sharp peaks confirming their crystallinity and an average size of 82.76 nm. FTIR spectroscopy identified extracellular protein compounds as capping agents. SEM examination revealed spherical agglomeration of the crystalline AgNPs. The antimicrobial assay by disc diffusion method, MIC and MBC testing revealed that the biosynthesized AgNPs showed moderate antibacterial activity against both pathogenic gram-negative (Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii) and gram-positive (Bacillus cereus, Staphylococcus aureus, and Streptococcus mutans) bacterial strains. Furthermore, the AgNPs significantly disrupted the biofilm of Pseudomonas aeruginosa, as confirmed by CV assay and fluorescent microscopy. Overall, this study underscores the potential of microbial-synthesized nanoparticles in biomedical applications, particularly in combating pathogenic bacteria, offering a promising avenue for future research and development.
PubMed: 38845375
DOI: 10.1093/lambio/ovae053 -
Frontiers in Microbiology 2020Anthropogenic perturbations introduce novel selective pressures to natural environments, impacting the genomic variability of organisms and thus altering the...
Anthropogenic perturbations introduce novel selective pressures to natural environments, impacting the genomic variability of organisms and thus altering the evolutionary trajectory of populations. Water overexploitation for agricultural purposes and defective policies in Cuatro Cienegas, Coahuila, Mexico, have strongly impacted its water reservoir, pushing entire hydrological systems to the brink of extinction along with their native populations. Here, we studied the effects of continuous water overexploitation on an environmental aquatic lineage of over a 13-year period which encompasses three desiccation events. By comparing the genomes of a population sample from 2003 (original state) and 2015 (perturbed state), we analyzed the demographic history and evolutionary response to perturbation of this lineage. Through coalescent simulations, we obtained a demographic model of contraction-expansion-contraction which points to the occurrence of an evolutionary rescue event. Loss of genomic and nucleotide variation alongside an increment in mean and variance of Tajima's , characteristic of sudden population expansions, support this observation. In addition, a significant increase in recombination rate (R/θ) was observed, pointing to horizontal gene transfer playing a role in population recovery. Furthermore, the gain of phosphorylation, DNA recombination, small-molecule metabolism and transport and loss of biosynthetic and regulatory genes suggest a functional shift in response to the environmental perturbation. Despite subsequent sampling events in the studied site, no pseudomonad was found until the lagoon completely dried in 2017. We speculate about the causes of final decline or possible extinction. Overall our results are evidence of adaptive responses at the genomic level of bacterial populations in a heavily exploited aquifer.
PubMed: 33552002
DOI: 10.3389/fmicb.2020.563885 -
Microbiology Resource Announcements Apr 2020We isolated strain MrB4 from the near-shore area of Lake Biwa in Japan and generated its complete genome sequence. MrB4 possesses a single circular chromosome of...
We isolated strain MrB4 from the near-shore area of Lake Biwa in Japan and generated its complete genome sequence. MrB4 possesses a single circular chromosome of 6,089,454 bp, with ∼97% average nucleotide identity to the type strain MCC10330 (draft genome).
PubMed: 32299875
DOI: 10.1128/MRA.00148-20 -
Journal of Water and Health May 2023Metallo-β-lactamases (MBLs) encoding carbapenem resistance in wastewater are a well-known serious threat to human health. Twelve Pseudomonas otitidis isolates obtained...
Metallo-β-lactamases (MBLs) encoding carbapenem resistance in wastewater are a well-known serious threat to human health. Twelve Pseudomonas otitidis isolates obtained from a municipal wastewater treatment plant (WWTP) in Hawaii were found to possess a subclass B3 MBL - POM (P. otitidis MBL), with a minimum inhibition concentration (MIC) range of 8-16 mg/L. The unrooted neighbor-joining phylogenetic tree showed that these bla genes isolated in wastewater samples (n = 12) were distinctly different from other reference genes isolated from clinical, freshwater, animal, and soil samples except for isolates MR7, MR8, and MR11. MR7, MR8, and MR11 were found to have 4, 3, and 3 amino acid substitutions when compared to the type strain MC10330 and were closely clustered to the clinical reference genes. The meropenem hydrolysis experiment showed that isolates with multiple amino acid substitutions completely hydrolyzed 64 mg/L of meropenem in 7 h. The emergence of the opportunistic pathogen P. otitidis chromosomally encoding bla in the treated municipal wastewater is an alarming call for the spread of this MBL in the environment. Further studies are required to understand the mechanism and regulation of this carbapenem-resistant β-lactamase in order to fill in the knowledge gap.
Topics: Animals; Humans; Carbapenems; Meropenem; Anti-Bacterial Agents; Wastewater; Phylogeny; beta-Lactamases; Microbial Sensitivity Tests; Pseudomonas aeruginosa
PubMed: 37254905
DOI: 10.2166/wh.2023.255 -
Bioresource Technology Nov 2012Polyhydroxyalkanoates (PHA) production using Pseudomonas otitidis, a newly isolated strain from PHA producing bioreactor was investigated using synthetic acids (SA) and...
Polyhydroxyalkanoates (PHA) production using Pseudomonas otitidis, a newly isolated strain from PHA producing bioreactor was investigated using synthetic acids (SA) and acidogenic effluents (AE) from biohydrogen reactor at different organic loading rates (OLRs). P. otitidis showed ability to grow and accumulate PHA, with simultaneous waste remediation. AE showed less PHA production (54%, OLR3), than SA (58%, OLR2). PHA composition showed co-polymer, poly-3(hydroxy butyrate-co-hydroxy valerate), P3(HB-co-HV). Bioprocess evaluation and enzymatic activities showed good correlation with PHA production. Kinetic studies on the growth of bacteria using different models at varying OLR were substantiated with PHA production. High substrate removal was registered at OLR1 (SA, 87%; AE, 82%). AE could be used as an alternative for pure substrates keeping in view of their high cost.
Topics: Acids; Base Sequence; Biocatalysis; Biological Oxygen Demand Analysis; Fatty Acids, Volatile; Hydrogen-Ion Concentration; Kinetics; Models, Biological; Molecular Sequence Data; Phylogeny; Polyhydroxyalkanoates; Pseudomonas; RNA, Ribosomal, 16S; Sequence Analysis, RNA; Substrate Specificity; Waste Disposal, Fluid; Wastewater; Water Purification
PubMed: 22940357
DOI: 10.1016/j.biortech.2012.07.077 -
Pathogens (Basel, Switzerland) Dec 2022is a rare and unique species among the genus that has not been previously reported as a cause of male genitourinary tract infection. In this report, we describe a case...
is a rare and unique species among the genus that has not been previously reported as a cause of male genitourinary tract infection. In this report, we describe a case of a 20-year-old immunocompetent male who presented with recurrent epididymo-orchitis, which was initially misidentified as Vibrio vulnificus and treated successfully. The causative agent could not be identified appropriately using the available routine methods, but a final identification was established using 16S rRNA targeted sequencing followed by whole-genome sequencing.
PubMed: 36558809
DOI: 10.3390/pathogens11121475