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Antimicrobial Agents and Chemotherapy Mar 2015The POM-1 metallo-β-lactamase is a subclass B3 resident enzyme produced by Pseudomonas otitidis, a pathogen causing otic infections. The enzyme was overproduced in...
The POM-1 metallo-β-lactamase is a subclass B3 resident enzyme produced by Pseudomonas otitidis, a pathogen causing otic infections. The enzyme was overproduced in Escherichia coli BL21(DE3), purified by chromatography, and subjected to structural and functional analysis. The purified POM-1 is a tetrameric enzyme of broad substrate specificity with higher catalytic activities with penicillins and carbapenems than with cephalosporins.
Topics: Anti-Bacterial Agents; Bacterial Proteins; Carbapenems; Catalysis; Cephalosporins; Escherichia coli; Penicillins; Pseudomonas; beta-Lactamases
PubMed: 25512428
DOI: 10.1128/AAC.03843-14 -
3 Biotech Mar 2022Production of biosurfactant by a novel indigenous isolate strain DU13 and its role in bioremediation of petroleum hydrocarbon is reported. The identity of the isolate...
Production of biosurfactant by a novel indigenous isolate strain DU13 and its role in bioremediation of petroleum hydrocarbon is reported. The identity of the isolate was confirmed by 16S rDNA gene sequencing analysis (Genbank accession: MK177190). The biosurfactant produced by the isolate could reduce the surface tension of petroleum supplemented medium by 46% just after 7 days of treatment. The emulsification index ( ) of the surfactant was found 37, 35, and 20%, respectively, against used motor oil, diesel, and kerosene. The FTIR spectrum of the crude biosurfactant showed the presence of υ stretch, υ υ stretch and υ bonding. The isolated strain could degrade 26% of TPH content of used motor oil in liquid culture. Whereas, ex situ pilot-scale field trial demonstrated very high bioremediation potential of the isolate in terms of germination rate of and seeds and plant growth just after 20 days of treatment.
PubMed: 35223354
DOI: 10.1007/s13205-022-03133-2 -
International Journal of Systematic and... Nov 2021Strain TUM18999 was isolated from the skin of a patient with burn wounds in Japan. The strain was successfully cultured at 20-42 °C (optimum, 30-35 °C) in 1.0-4.0%...
Strain TUM18999 was isolated from the skin of a patient with burn wounds in Japan. The strain was successfully cultured at 20-42 °C (optimum, 30-35 °C) in 1.0-4.0% NaCl (w/v) and at pH 5.5-9.5, optimum pH 5.5-8.5. The phylogenetic tree reconstructed using 16S rRNA, , and gene sequences indicated that strain TUM18999 is closely related to MCC10330. Although the partial 16S rRNA gene sequence (1412 bp) of TUM18999 exhibits high similarity to those of NBRC 14159 (99.08 %) and MCC10330 (98.51 %), multi-locus sequence analysis using 16S rRNA, , and genes reveals a clear distinction between TUM18999 and other species. In addition, an average nucleotide identity >90 % was not observed in the group. Moreover, TUM18999 and can be distinguished based on the minimum inhibitory concentration for carbapenem. Meanwhile, the cellular fatty acids are enriched with C 7/C 6 (34.35 %), C 7/C 6 (24.22 %), C (19.79 %) and C (8.25 %). Based on this evidence, strain TUM18999 can be defined as representing a novel species, with the proposed name sp. nov. The type strain is TUM18999 (GTC 22698=NCTC 14580).
Topics: Bacterial Typing Techniques; Base Composition; Burns; DNA, Bacterial; Fatty Acids; Genes, Bacterial; Humans; Japan; Phylogeny; Pseudomonas; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Skin
PubMed: 34762579
DOI: 10.1099/ijsem.0.005115 -
Journal of Global Antimicrobial... Dec 2016
Topics: Aged; Anti-Bacterial Agents; Fasciitis, Necrotizing; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Peritonitis; Pseudomonas; Pseudomonas Infections; Republic of Korea; beta-Lactamases
PubMed: 27835844
DOI: 10.1016/j.jgar.2016.09.006 -
Archives of Microbiology Jul 2020L-asparaginase (E.C.3.5.1.1) is an important enzyme that has been purified and characterized for over decades to study and evaluate its anti-carcinogenic activity... (Review)
Review
L-asparaginase (E.C.3.5.1.1) is an important enzyme that has been purified and characterized for over decades to study and evaluate its anti-carcinogenic activity against different lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and Hodgkin's lymphoma. The ability of the enzyme to convert L-asparagine into aspartic acid and ammonia is the reason behind its anti-cancerous activity. Apart from its medicinal uses, it is widely used in food industry to tackle acrylamide, a probable human carcinogen and, production in carbohydrate-rich foods cooked at high temperatures. There are variety of organisms including microorganisms such as bacteria, fungi, algae, and plants that produce L-asparaginase. The enzyme obtained from different microbial and plant sources have different physiochemical properties and kinetic parameters. L-asparaginases have an optimum pH range between 6 and 10 and an optimum temperature between 37 and 85 °C. This article has reviewed the lowest molecular mass for L-asparaginase in Yersinia pseudotuberculosis Q66CJ2 which is 36.27 kDa, while the highest for Pseudomonas otitidis which has a molecular mass of 205 ± 3 kDa. This review is an attempt to summarize most of the available sources, their phylogenetic relationships, purification methods, data regarding different physiochemical and kinetic properties of L-asparaginase.
Topics: Ammonia; Asparaginase; Asparagine; Aspartic Acid; Bacteria; Fungi; Hodgkin Disease; Humans; Phylogeny; Plants; Precursor Cell Lymphoblastic Leukemia-Lymphoma
PubMed: 32052094
DOI: 10.1007/s00203-020-01814-1 -
Biotechnologia 2021Melanin finds enormous applications in different industries for its unique photoprotective and anti-oxidant properties. Due to its emerging demand, scientific...
BACKGROUND
Melanin finds enormous applications in different industries for its unique photoprotective and anti-oxidant properties. Due to its emerging demand, scientific researchers are putting efforts to unravel more microorganisms with a potential of producing melanin on large scale. Hence, the present study was aimed at the isolation of extracellular melanin producing microorganisms from lime quarries of Karnataka, India. Besides this, the tyrosinase gene governing melanin synthesis in different organisms were compared to understand its evolutionary aspects.
MATERIAL AND METHODS
Melanin producing microorganisms were screened on tyrosine gelatin beef extract agar medium. Potential isolate was explored for submerged production of melanin in broth containing L-tyrosine. Melanin was characterized by UV-Vis spectroscopy, thin layer and high performance liquid chromatographic techniques. Antibacterial activity of melanin was performed by agar well assay. Comparative tyrosinase gene sequence analysis was performed by using Geneious 2021.1 trial version software.
RESULTS
DDB2 was found to be potential for melanin production. No antibacterial activity was exerted by the melanin against tested pathogens. The studies showed that the common central domain of tyrosinase protein sequence of selected sps. exhibited 100% identity with the common central domain of tyrosinase (NP_000363.1).
CONCLUSIONS
Our study shows the production of melanin in good quantities by the isolate DDB2 which can be explored for scale-up process. Since the melanin formed is of eumelanin type and the tyrosinase gene sequence of several sp. showed relatedness to humans, this molecule may be further developed for sunscreen formulations.
PubMed: 36605604
DOI: 10.5114/bta.2021.111106 -
Journal of Global Antimicrobial... Jun 2015Pseudomonas spp. are ubiquitous in nature. Carbapenem resistance in environmental isolates of members of this genus is thought to be rare but the exact resistance rate...
Pseudomonas spp. are ubiquitous in nature. Carbapenem resistance in environmental isolates of members of this genus is thought to be rare but the exact resistance rate is unknown. In this study, carbapenem-resistant Pseudomonas spp. were isolated from chicken and pork samples and the mechanisms underlying the carbapenem resistance in these strains were investigated. A total of 16 carbapenem-resistant Pseudomonas aeruginosa, Pseudomonas putida and Pseudomonas otitidis isolates were recovered from eight samples of chicken and pork. The isolates exhibited meropenem minimum inhibitory concentrations (MICs) of 8 to ≥32mg/L and imipenem MICs of <0.5-16mg/L yet did not harbour any acquired carbapenemase genes. Meropenem resistance in various strains was found to be mediated by efflux systems only, whereas overexpression of MexAB-OprM efflux pump and lack of OprD porin were responsible for carbapenem resistance in P. aeruginosa. The intrinsic metallo-β-lactamase gene bla in P. otitidis and overexpression of the TtgABC efflux system in P. putida were also responsible for carbapenem resistance in these organisms. In conclusion, this study reports for the first time the isolation of carbapenem-resistant P. aeruginosa, P. otitidis and P. putida strains from food. The resistance mechanisms of these strains are rarely due to production of carbapenemases. Further selection of such carbapenem-resistant Pseudomonas spp. in the environment and the risk by which they are transmitted to clinical settings are of great public health concern.
PubMed: 27873658
DOI: 10.1016/j.jgar.2015.03.006 -
Microorganisms Jun 2021The rhizobacterium AVO110 exhibits antagonism toward the phytopathogenic fungus . This strain efficiently colonizes hyphae and is able to feed on their exudates. Here,...
The rhizobacterium AVO110 exhibits antagonism toward the phytopathogenic fungus . This strain efficiently colonizes hyphae and is able to feed on their exudates. Here, we report the complete genome sequence of AVO110. The phylogeny of all available genomes separates environmental isolates, including AVO110, from those obtained from infected human blood and oyster tissues, which cluster together with . Core and pan-genome analyses showed that strains encode highly heterogenic gene pools, with the AVO110 genome encoding the largest and most exclusive variable region (~1.6 Mb, 1795 genes). The AVO110 singletons include a wide repertoire of genes related to biofilm formation, several of which are transcriptionally modulated by exudates. One of these genes () encodes a GGDEF/EAL domain protein specific to spp. strains isolated primarily from the rhizosphere of diverse plants, but also from soil and water samples. We also show that CmpA has a role in biofilm formation and that the integrity of its EAL domain is involved in this function. This study contributes to a better understanding of the niche-specific adaptations and lifestyles of , including the mycophagous behavior of strain AVO110.
PubMed: 34202389
DOI: 10.3390/microorganisms9071388 -
Journal of Environmental Sciences... 2009Pseudomonas otitidis WL-13, which has a high capacity to decolorize triphenylmethane dyes, was isolated from activated sludge obtained from a wastewater treatment plant...
Pseudomonas otitidis WL-13, which has a high capacity to decolorize triphenylmethane dyes, was isolated from activated sludge obtained from a wastewater treatment plant of a dyeing industry. This strain exhibited a remarkable color-removal capability when tested against several triphenylmethane dyes under both shaking and static conditions at high concentrations of dyes. More than 95% of Malachite Green and Brilliant Green was removed within 12 h at 500 micromol/L dye concentration under shaking conditions. Crystal Violet lost about 13% of its color under the same conditions tested. The rate of decolorization increased when the M9 medium was supplemented with yeast extract. The optimum pH and temperature for color removal were 7-9 and 35-40 degrees C, respectively. The observed changes in the visible spectra and the inspection of bacterial growth indicated the color-removal by the adsorption of dye to the cells during incubation with strains.
Topics: Biodegradation, Environmental; Coloring Agents; Pseudomonas; Trityl Compounds
PubMed: 19862963
DOI: 10.1016/s1001-0742(08)62368-2 -
Diagnostic Microbiology and Infectious... Mar 2012
Topics: Anti-Bacterial Agents; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Molecular Typing; Otitis Media; Pseudomonas; Pseudomonas Infections; beta-Lactamases
PubMed: 22209511
DOI: 10.1016/j.diagmicrobio.2011.11.007