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Journal of Clinical Immunology Jan 2014A 16-year old boy with chronic granulomatous disease (CGD) developed Psychrobacter immobilis septicemia during a course of fulminant hepatic failure. The patient died...
A 16-year old boy with chronic granulomatous disease (CGD) developed Psychrobacter immobilis septicemia during a course of fulminant hepatic failure. The patient died despite aggressive management with antimicrobials and corticosteroids. While Psychrobacter immobilis rarely affects humans, it should be considered an organism that can cause sepsis in patients with CGD.
Topics: Adolescent; Anti-Bacterial Agents; Autopsy; Bacteremia; Fatal Outcome; Granulomatous Disease, Chronic; Humans; Liver Failure, Acute; Male; Moraxellaceae Infections; Psychrobacter
PubMed: 24217814
DOI: 10.1007/s10875-013-9961-7 -
Journal of Clinical Microbiology Oct 1987Thirty strains of microorganisms previously classified in the CDC group EO-2 were studied to determine the relationship of this group and the recently proposed... (Comparative Study)
Comparative Study
Thirty strains of microorganisms previously classified in the CDC group EO-2 were studied to determine the relationship of this group and the recently proposed Psychrobacter immobilis. Results of transformation studies indicated that 9 of the 30 studied strains should be reclassified as P. immobilis. While transformation studies should be used to identify the Psychrobacter genus definitively, some presumptive characteristics useful in differentiating the CDC group EO-2 and P. immobilis were observed. The Psychrobacter genus, compared with the CDC group EO-2, grew well at 25 degrees C, lightly or not at all at 35 degrees C, and not at all at 42 degrees C, grew in nutrient broth without NaCl and with only one exception grew in 6% NaCl, usually did not produce acid from adonitol, dextrin, fructose, or D-mannitol, did not demonstrate the O-shaped cellular morphology, and usually possessed an odor resembling that noted from a phenylethyl alcohol blood agar plate.
Topics: DNA, Bacterial; Gram-Negative Bacteria; Humans; Transformation, Bacterial
PubMed: 3667912
DOI: 10.1128/jcm.25.10.1907-1910.1987 -
International Journal of Systematic and... Mar 2012Human Psychrobacter isolates, other than Psychrobacter phenylpyruvicus, are predominantly designated Psychrobacter immobilis. Phenotypic and genotypic testing of...
Psychrobacter isolates of human origin, other than Psychrobacter phenylpyruvicus, are predominantly Psychrobacter faecalis and Psychrobacter pulmonis, with emended description of P. faecalis.
Human Psychrobacter isolates, other than Psychrobacter phenylpyruvicus, are predominantly designated Psychrobacter immobilis. Phenotypic and genotypic testing of Psychrobacter isolates that have been deposited in different culture collections as P. immobilis indicates that most of these human isolates belong to the species Psychrobacter faecalis and Psychrobacter pulmonis.
Topics: Bacterial Typing Techniques; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Humans; Molecular Sequence Data; Moraxellaceae Infections; Phylogeny; Psychrobacter; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 21551328
DOI: 10.1099/ijs.0.032631-0 -
AIDS (London, England) Aug 1994
Topics: AIDS-Related Opportunistic Infections; Acquired Immunodeficiency Syndrome; Adult; Fatal Outcome; Gram-Negative Aerobic Bacteria; Gram-Negative Bacterial Infections; Humans; Male; Zidovudine
PubMed: 7986423
DOI: 10.1097/00002030-199408000-00027 -
Journal of Clinical Microbiology Sep 1991Psychrobacter immobilis was isolated from the cerebrospinal fluid (CSF) and blood of a 2-day-old infant who appeared well except for a fever and a full anterior...
Psychrobacter immobilis was isolated from the cerebrospinal fluid (CSF) and blood of a 2-day-old infant who appeared well except for a fever and a full anterior fontanelle. The infant was treated with antibiotics intravenously. After 48 h, he became afebrile and CSF and blood cultures were negative; he was then discharged. After 96 h of incubation, CSF and blood cultures yielded a gram-negative organism, P. immobilis. The child was readmitted to the hospital, and the same organism was again isolated from his blood and CSF.
Topics: Humans; Infant, Newborn; Male; Meningitis, Bacterial; Neisseriaceae; Neisseriaceae Infections
PubMed: 1774332
DOI: 10.1128/jcm.29.9.2041-2042.1991 -
International Journal of Systematic and... Jul 2003Eleven psychrophilic bacteria isolated from Antarctic coastal marine environments were subjected to a polyphasic taxonomic study. The isolates were oxidase-positive,...
Eleven psychrophilic bacteria isolated from Antarctic coastal marine environments were subjected to a polyphasic taxonomic study. The isolates were oxidase-positive, halotolerant, gram-negative, non-motile coccobacilli with a strictly oxidative metabolism. The DNA G + C content ranged from 44 to 47 mol%. DNA-DNA hybridization experiments showed six homology groups, two of them related at the species level to the type strain of Psychrobacter immobilis, LMG 7203T (70-83%). The highest DNA relatedness of two other groups to known Psychrobacter species was found to the type strain of Psychrobacter glacincola, LMG 21282T (51-57%), and no significant similarity was found between Psychrobacter type strains and the last two groups. The predominant cellular fatty acids detected were typical of the genus Psychrobacter and included 18:1omega9c, 16:1omega7c and 17:1omega8c. 16S rRNA gene sequence analysis confirmed that the strains isolated belonged to the genus Psychrobacter. The results of the study assigned five isolates to P. immobilis, three isolates to P. glacincola and three isolates to novel Psychrobacter species. The names Psychrobacter luti sp. nov. (type strain NF11T=LMG 21276T=CECT 5885T) and Psychrobacter fozii sp. nov. (type strain NF23T=LMG 21280T=CECT 5889T) are proposed for these organisms.
Topics: Antarctic Regions; Base Composition; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Gammaproteobacteria; Microscopy, Electron; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Bacterial; RNA, Ribosomal, 16S
PubMed: 12892132
DOI: 10.1099/ijs.0.02457-0 -
Applied and Environmental Microbiology Dec 1995A class C beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile Psychrobacter immobilis A8. This psychrophilic beta-lactamase...
A class C beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile Psychrobacter immobilis A8. This psychrophilic beta-lactamase displays a low level of thermal stability and a low optimal temperature of activity. In contrast to other cold-adapted enzymes, its level of specific activity is not higher than that of mesophilic class C beta-lactamases.
Topics: Acinetobacter; Amino Acid Sequence; Molecular Sequence Data; Temperature; beta-Lactamases
PubMed: 8534113
DOI: 10.1128/aem.61.12.4474-4476.1995 -
Biochimica Et Biophysica Acta Jul 1995
Corrigendum to "Cloning, sequence and structural features of a lipase from the antarctic facultative psychrophile Psychrobacter immobilis B10" [Biochim. Biophys. Acta 1171 (1993) 331-333].
Topics: Amino Acid Sequence; Bacteria; Base Sequence; Cold Temperature; Genes, Bacterial; Lipase; Molecular Sequence Data
PubMed: 7632728
DOI: 10.1016/0167-4781(95)00086-v -
International Journal of Systematic and... Mar 2003Unusual Gram-negative, catalase- and oxidase-positive, coccus-shaped bacteria isolated from the lungs of two lambs were characterized by phenotypic and molecular-genetic...
Unusual Gram-negative, catalase- and oxidase-positive, coccus-shaped bacteria isolated from the lungs of two lambs were characterized by phenotypic and molecular-genetic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown isolates were genealogically highly related to each other (99.8% sequence similarity) and represent a novel subline within the genus Psychrobacter. The unknown bacterium was phylogenetically closely related to, but distinct from, Psychrobacter phenylpyruvicus, Psychrobacter immobilis, Psychrobacter glacincola and Psychrobacter urativorans. The novel Psychrobacter isolates were readily distinguished from all other Psychrobacter species and other Gram-negative, oxidase-positive bacteria usually responsible for lung infections in sheep by physiological and biochemical tests. Based on molecular-genetic and phenotypic evidence, it is proposed that the unknown Psychrobacter isolates from lambs be classified as Psychrobacter pulmonis sp. nov. The type strain is strain S-606T (=CECT 5989T =CCUG 46240T).
Topics: Animals; Gammaproteobacteria; Gram-Negative Bacterial Infections; Lung; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Sheep; Sheep Diseases
PubMed: 12710606
DOI: 10.1099/ijs.0.02413-0 -
Journal of Clinical Microbiology Mar 1988We determined phenotypic characteristics, cellular fatty acid composition, and isoprenoid quinone content of representative strains of CDC groups EO-2, M-5, and M-6,...
Cultural and chemical characterization of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis.
We determined phenotypic characteristics, cellular fatty acid composition, and isoprenoid quinone content of representative strains of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis. All organisms contained ubiquinone with eight isoprene units as the major isoprenolog, but distinct differences were observed in fatty acid composition. Twenty-eight of the original collection of CDC group EO-2 strains were further identified as P. immobilis, EO-2, or EO-3 by distinctive cellular fatty acid profiles, cellular morphology, and pigment production. The cellular fatty acid compositions of M-5 and M-6 were similar but were clearly different from those of other organisms. The genus Acinetobacter was differentiated from other organisms in the study by small amounts of 2-hydroxydodecanoic acid (2-OH-12:0), and P. immobilis was differentiated by small amounts of decanoic acid (10:0) and a branched-chain 17-carbon acid (i-17:0). All Moraxella species were distinguished by small amounts of decanoic acid (10:0) and the absence of i-17:0. M. bovis, M. nonliquefaciens, and some strains of M. lacunata formed a single fatty acid group, while M. osloensis, M. phenylpyruvica, M. atlantae, and other strains of M. lacunata (M. lacunata II) had species-specific fatty acid profiles. O. urethralis differed from Moraxella species by the presence of large amounts (49%) of cis-vaccenic acid (18:1 omega 7c), small amounts (1%) of 3-hydroxyhexadecanoate (3-OH-16:0), and the absence of 10:0 and 3-hydroxydodecanoate (3-OH-12:0). The combined use of chemical data and a small number of conventional tests permitted rapid identification and differentiation of these organisms from each other and from related organisms.
Topics: Acinetobacter; Animals; Chromatography, Gas; Fatty Acids; Gram-Negative Bacteria; Humans; Minicomputers; Moraxella; Software; Transformation, Bacterial
PubMed: 3356788
DOI: 10.1128/jcm.26.3.484-492.1988