-
Biochimica Et Biophysica Acta Aug 1971
Topics: Acetates; Amino Acids; Anti-Bacterial Agents; Carbon Isotopes; Chloramphenicol; Chromatography, Thin Layer; Cycloheximide; Dansyl Compounds; Electrophoresis; Formates; Kinetics; Metabolism; Methionine; Peptide Biosynthesis; Protein Biosynthesis; Puromycin; Pyrrolidines; Radioisotopes; Saccharomyces; Sodium; Tritium
PubMed: 5132901
DOI: 10.1016/0005-2787(71)90130-4 -
Journal of Molecular Biology Apr 1967
Topics: Electrophoresis; Methionine; Polynucleotides; Puromycin; Ribosomes
PubMed: 6034103
DOI: 10.1016/0022-2836(67)90146-5 -
Somatic Cell Genetics Jul 1982We have isolated from an established Aedes albopictus (mosquito) cell line clones which are resistant to the antibiotic puromycin. On the basis of growth and plating...
We have isolated from an established Aedes albopictus (mosquito) cell line clones which are resistant to the antibiotic puromycin. On the basis of growth and plating efficiency, clones Pur-8026 and Pur-8612 were five- and seven-fold more resistant, respectively, to puromycin than wild-type cells. In vitro protein synthesis was resistant to puromycin only in extracts prepared from Pur-8612 cells. Measurements of puromycin transport, cross-resistance to colchicine, and sensitivity to Tween-80 indicating that resistance in Pur-8026 cells was due to membrane alteration(s) affecting permeability to puromycin. This is the first description of puromycin resistant in insect cells and also the first report of puromycin resistance in an animal cell variant associated with an alteration at the level of protein synthesis.
Topics: Aedes; Animals; Biological Transport; Cell Line; Cell Separation; Clone Cells; Colchicine; Drug Resistance; Protein Biosynthesis; Puromycin; Selection, Genetic; Time Factors
PubMed: 7123454
DOI: 10.1007/BF01538712 -
Methods in Enzymology 1975
Topics: Chromatography; Chromatography, DEAE-Cellulose; Chromatography, Gel; Culture Media; Electrophoresis, Paper; Hydrogen-Ion Concentration; Kinetics; Methods; Methyltransferases; Puromycin; S-Adenosylmethionine; Silicon Dioxide; Streptomyces; Time Factors
PubMed: 237215
DOI: 10.1016/0076-6879(75)43112-3 -
Proceedings of the National Academy of... Aug 1975[3H]Puromycin and N-(ethyl-2-diazomalonyl)[3H]puromycin are incorporated into E. coli ribosomes on irradiation at 253.7 nm. Both compounds incorporate into both protein...
[3H]Puromycin and N-(ethyl-2-diazomalonyl)[3H]puromycin are incorporated into E. coli ribosomes on irradiation at 253.7 nm. Both compounds incorporate into both protein and nucleic acid. Two-dimensional gel electrophoresis of ribosomal protein shows that L23 is the major protein labeled by puromycin. Although incorporation is clearly a complex process, evidence is presented that L23 is labeled via an affinity labeling process, thus placing L23 at the aminoacyl-tRNA receptor (A) site. N-(ethyl-2-diazomalonyl)puromycin is a ribosomal ligand, as shown by its inhibition of two ribosomal assays, but it is not a good puromycin analog, and it is unclear whether its incorporation, which proceeds via both carbene-dependent and carbene-independent processes, results from affinity labeling.
Topics: Azo Compounds; Escherichia coli; Kinetics; Malonates; Photochemistry; Puromycin; Ribosomal Proteins; Ribosomes
PubMed: 1103130
DOI: 10.1073/pnas.72.8.2974 -
Journal of the American Chemical Society Mar 2014The cleavage of a photolabile nitroveratryloxycarbonyl protecting group, which is widely used as caging group, was studied by femtosecond transient absorption...
The cleavage of a photolabile nitroveratryloxycarbonyl protecting group, which is widely used as caging group, was studied by femtosecond transient absorption spectroscopy in the visible and infrared spectral range and by flash-photolysis experiments on the longer time scale. On the basis of quantum-chemical calculations it is shown that directly after excitation, triplet absorption that is not part of the reactive pathway dominates the transient spectrum and that the molecules following the triplet pathway are trapped in a nonreactive triplet state. By contrast, photolysis proceeds from the singlet manifold. Therefore, trapping in the triplet state lowers the quantum yield of the process for this compound compared with other o-nitrobenzyl protecting groups. With our integrated approach of time-resolved UV and IR measurements and calculations, we can characterize the entire uncaging mechanism and identify the most relevant intermediate states along the reaction pathway. The final uncaging is accomplished within 32 μs.
Topics: Absorption; Anti-Bacterial Agents; Models, Molecular; Molecular Conformation; Nitro Compounds; Photolysis; Puromycin; Quantum Theory
PubMed: 24517286
DOI: 10.1021/ja410594y -
Proceedings of the National Academy of... Jan 1971Previous experience has led us to suggest that sustained blockage of memory in mice upon administration of puromycin is caused by the persistence of peptidyl-puromycin...
Previous experience has led us to suggest that sustained blockage of memory in mice upon administration of puromycin is caused by the persistence of peptidyl-puromycin at synapses. The block can be removed and memory restored by intracerebral injections of water and by subcutaneous or intraperitoneal injections of several psychotropic drugs. The present experiments provide evidence that peptidyl-puromycin persists for long periods in subcellular fractions of synaptosomes (nerve endings), and that the peptide is lost from synaptosomal, but not from mitochondrial, fractions when memory is restored. These findings strengthen our interpretation of the mode of action of puromycin on memory.
Topics: Acetylcholinesterase; Animals; Brain; Depression, Chemical; Memory; Mice; Microscopy, Electron; Mitochondria; Nerve Endings; Peptides; Puromycin; Tritium
PubMed: 5276297
DOI: 10.1073/pnas.68.1.26 -
Biochemical and Biophysical Research... May 2003Puromycin is a well-known antibiotic that inhibits protein synthesis by competitive incorporation against an aminoacyl tRNA on the ribosome A site. Novel technology...
Puromycin is a well-known antibiotic that inhibits protein synthesis by competitive incorporation against an aminoacyl tRNA on the ribosome A site. Novel technology using this property of puromycin has been developed for convenient handling methods in protein research. Puromycin modified with another molecule is incorporated into a protein at the C-terminus, thus linking the desired molecule to the protein. Combination of in vitro translation with puromycin analogues has resulted in novel technologies such as display technology for screening, fluorescence labeling, affinity purification, and protein chip for proteomics.
Topics: Biotechnology; Chromatography, Affinity; Fluorescent Dyes; Models, Genetic; Peptide Library; Protein Array Analysis; Protein Biosynthesis; Protein Synthesis Inhibitors; Proteins; Proteomics; Puromycin; Viruses
PubMed: 12732187
DOI: 10.1016/s0006-291x(03)00686-7 -
Chemical Communications (Cambridge,... Jul 2017We have developed a versatile antibody-assisted strategy for the imaging and profiling of newly synthesized proteomes in a cell-specific manner. This strategy remained...
We have developed a versatile antibody-assisted strategy for the imaging and profiling of newly synthesized proteomes in a cell-specific manner. This strategy remained highly selective even in heterogeneous co-cultured cells, thus enabling labeling and enrichment of nascent proteomes from targeted cells without the need for physical separation.
Topics: Animals; Antibodies; CHO Cells; Cell Line, Tumor; Coculture Techniques; Cricetulus; Humans; Mice; Molecular Imaging; NIH 3T3 Cells; Proteome; Puromycin
PubMed: 28702584
DOI: 10.1039/c7cc04536k -
Science (New York, N.Y.) Nov 1969Intracerebral injections of puromycin produced memory deficits in naive quail trained to discriminate between red and green stimuli. Puromycin aminonucleoside,...
Intracerebral injections of puromycin produced memory deficits in naive quail trained to discriminate between red and green stimuli. Puromycin aminonucleoside, acetoxycycloheximide, and saline had no such effect. After a single reversal of the visual cues, naive quail treated with puromycin performed better than control birds. Also, puromycin had no effect on performance when injected into previously trained animals. High doses both of puromycin and acetoxycycloheximide inhibited ribonucleic acid and protein synthesis to a similar extent, while low doses of puromycin inhibited only protein synthesis. Since only puromycin inhibited memory, the basis for its effect appears more likely to be mediated by the action of peptidyl-puromycin rather than by the quantitative inhibition of macromolecular synthesis or by some nonspecific toxic action.
Topics: Animals; Birds; Brain; Cyclohexanes; Depression, Chemical; Discrimination Learning; Memory; Protein Biosynthesis; Puromycin; Pyridones; RNA
PubMed: 5348288
DOI: 10.1126/science.166.3909.1165