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Scandinavian Journal of... 1991Histamine is formed by decarboxylation of the amino acid histidine and is found both in plants and in animals, including man. In man it has important biologic functions.... (Review)
Review
Histamine is formed by decarboxylation of the amino acid histidine and is found both in plants and in animals, including man. In man it has important biologic functions. To assess the physiologic role of histamine, however, it is necessary to have a reliable and convenient method to determine its concentration in biologic fluids and tissue. Histamine has been determined by bioassay, chemically by different modification of a fluorometric method, by radioenzymatic methods, and, recently, by immunoassays. Immunoassay of histamine has, however, been difficult to establish, mainly as a result of problems with the production of an antibody with histamine specificity. This is due to the general occurrence of histamine in all animal species. By binding histamine to different ligands, several researchers have succeeded in producing antibodies against antigens in which histamine is integrated. Treating samples and histamine standard with the same coupling agent, reliable and specific radioimmunoassays of histamine have been established. We have for some years utilized a commercial radioimmunoassay of histamine and confirmed its convenience, specificity, and sensitivity. In some patients taking a histamine-2 blocker (cimetidine or ranitidine) we have detected an increase in plasma histamine which also tended to be increased after proximal gastric vagotomy and in patients with gastric ulcer compared with patients with duodenal ulcer. In rats treated with high doses of omeprazole for 90 days we found an increase in the enterochromaffin-like cell mass and in histamine concentration in the oxyntic mucosa which was reflected by an increase in plasma histamine.(ABSTRACT TRUNCATED AT 250 WORDS)
Topics: Animals; Histamine; Humans; Immunoassay; Radioimmunoassay; Sensitivity and Specificity
PubMed: 2042035
DOI: 10.3109/00365529109093175 -
Seminars in Nuclear Medicine Apr 1975The powerful nuclear medicine tool of radioimmunoassay was very rapidly developed because of the favorable properties of insulin in such a procedure. The current method...
The powerful nuclear medicine tool of radioimmunoassay was very rapidly developed because of the favorable properties of insulin in such a procedure. The current method of insulin immunoassay in our laboratory is described, with emphasis on the details of the separation of bound and free using talc tablets; emphasis is also given to the correction for tracer damage. The clinical usefulness of the insulin radioimmunoassay is somewhat less than had been anticipated, although its use as a research tool has had immeasurable impact upon the study of metabolism. The presence of proinsulin in plasma is a factor that has to be considered during the radioimmunoassay. Radioimmunoassays for the connecting peptide of proinsulin have also been developed. A preliminary evaluation of two radioimmunoassay kits is described.
Topics: Adenoma, Islet Cell; Diabetes Mellitus; Evaluation Studies as Topic; Humans; Insulin; Proinsulin; Radioimmunoassay
PubMed: 164696
DOI: 10.1016/s0001-2998(75)80032-8 -
CRC Critical Reviews in Clinical... Jun 1977
Review
Topics: Antibody Specificity; Antigens; Epitopes; Iodine Radioisotopes; Radioimmunoassay
PubMed: 71227
DOI: 10.3109/10408367709151689 -
Lancet (London, England) Apr 1989
Topics: Adult; Endothelins; Humans; Peptides; Radioimmunoassay
PubMed: 2564998
DOI: 10.1016/s0140-6736(89)92915-2 -
Revista de Investigacion Clinica;... 1973
Topics: Radioimmunoassay
PubMed: 4805168
DOI: No ID Found -
Gastroenterology Sep 1978
Topics: Periodicals as Topic; Publishing; Radioimmunoassay; United States
PubMed: 680516
DOI: No ID Found -
Nihon Rinsho. Japanese Journal of... Jun 1979
Topics: Humans; Radioimmunoassay
PubMed: 490925
DOI: No ID Found -
Rinsho Byori. the Japanese Journal of... Aug 1986
Topics: Radioimmunoassay; Reagent Kits, Diagnostic
PubMed: 3784030
DOI: No ID Found -
JPMA. the Journal of the Pakistan... Dec 1993
Topics: Radioimmunoassay
PubMed: 8133641
DOI: No ID Found -
Human Pathology Jul 1977
Topics: Economics; Immunoenzyme Techniques; Radioimmunoassay; Time Factors
PubMed: 330385
DOI: 10.1016/s0046-8177(77)80001-4