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Parasitology International Apr 2019Retortamonas spp. has been reported as an intestinal parasite among various host organisms, including humans; however, its intra-genus molecular diversity has not yet...
Retortamonas spp. has been reported as an intestinal parasite among various host organisms, including humans; however, its intra-genus molecular diversity has not yet been elucidated. Haplotypes of the 18S small subunit ribosomal RNA locus (1836-1899 bp) of Retortamonas spp. from humans (n = 8), pigs (n = 6), dogs (n = 1), goats (n = 16), water buffalos (n = 23), cattle (n = 7), rats (n = 3), and chickens (n = 5) were analyzed with references isolated from non-human mammals, amphibians, and insects. Phylogenetic and network analyses revealed a statistically supported three cluster formation among the vertebrate-isolated haplotypes, while insect-isolated haplotypes were independently clustered with Chilomastix. In the clade of vertebrate isolates, assemblage A (amphibian genotype), which included the amphibian references, was addressed as an out-group of the other clusters. Assemblage B (mammalian and chicken genotype) included most haplotypes from various mammals including humans with the haplotypes isolated from a chicken. Human isolates were all classified into this assemblage, thus assemblage B might correspond to R. intestinalis. Assemblage C (bovine genotype), which included specific haplotypes from water buffalos and cattle, was addressed as a sister lineage of assemblage B. Among the diversified haplotypes of assemblage B, a specific haplotype, which was identified from multiple host mammals (humans, dogs, pigs, cattle, water buffalos, elks, goats, and rats), indicates the potential zoonotic transmission of the Retortamonas among them. The genotyping classification of retortamonads could contribute to a better understanding of its molecular epidemiology, especially among humans and related host organisms.
Topics: Animals; Buffaloes; Cattle; Chickens; DNA, Protozoan; Dogs; Feces; Gene Regulatory Networks; Genotype; Goats; Haplotypes; Humans; Insecta; Intestines; Phylogeny; Protozoan Proteins; RNA, Ribosomal; Rats; Retortamonadidae; Swine; Zoonoses
PubMed: 30550977
DOI: 10.1016/j.parint.2018.12.004 -
Revista Brasileira de Parasitologia... 2023In vitro excystation of cysts of microscopically identified Chilomastix mesnili and Retortamonas sp. isolated from Japanese macaques and Retortamonas sp. isolated from...
In vitro excystation of cysts of microscopically identified Chilomastix mesnili and Retortamonas sp. isolated from Japanese macaques and Retortamonas sp. isolated from small Indian mongooses could be induced using an established protocol for Giardia intestinalis and subsequently by culturing with H2S-rich Robinson's medium supplemented with Desulfovibrio desulfuricans. Excystation usually began 2 h after incubation in Robinson's medium. DNA was isolated from excysted flagellates after 4 h of incubation or from cultured excysted flagellates. Phylogenetic analysis based on their 18S rRNA genes revealed that two isolates of C. mesnili from Japanese macaques belonged to the same cluster as a C. mesnili isolate from humans, whereas a mammalian Retortamonas sp. isolate from a small Indian mongoose belonged to the same cluster as that of an amphibian Retortamonas spp. isolate from a 'poison arrow frog' [sequence identity to AF439347 (94.9%)]. These results suggest that the sequence homology of the 18S rRNA gene of the two C. mesnili isolates from Japanese macaques was similar to that of humans, in addition to the morphological similarity, and Retortamonas sp. infection of the amphibian type in the small Indian mongoose highlighted the possibility of the effect of host feeding habitats.
Topics: Humans; Animals; Phylogeny; Retortamonadidae; Herpestidae; Macaca fuscata; Parasites; RNA, Ribosomal, 18S
PubMed: 38055438
DOI: 10.1590/S1984-29612023070 -
Gastrointestinal Endoscopy Apr 2001
Topics: Animals; Cholangiopancreatography, Endoscopic Retrograde; Eukaryota; Humans; Middle Aged; Pancreatic Diseases; Pancreatic Ducts; Pancreatic Juice
PubMed: 11275898
DOI: 10.1067/mge.2001.112746 -
Journal of Water and Health Apr 2019To determine the role of soils in dissemination of enteric protozoan cysts and intestinal eggs and/or larvae of helminths, a study was carried out on muddy soils around...
To determine the role of soils in dissemination of enteric protozoan cysts and intestinal eggs and/or larvae of helminths, a study was carried out on muddy soils around springs and wells of six districts in Yaounde, Cameroon from February to July 2015. Protozoan cysts and helminth eggs and larvae were observed microscopically after concentration by standard scientific methods. Flagellated enteric protozoa cysts were detected at an average density of 245 ± 145 cysts/L for Giardia intestinalis, 308 ±190 cysts/L for Chilomastix mesnili, 105 ± 106 cysts/L for Enteromonas hominis and 96 ± 118 cysts/L for Retortamonas intestinalis. Cyst densities were higher during the short rainy season (277 ± 119 cysts/L) than in the short dry season (147 ± 60 cysts/L). The helminths identified were Ascaris sp., Enterobius sp., Necator americanus and/or Ancylostoma duodenale, Strongyloides sp., Taenia sp., Hymenolepis sp., Diphyllobothrium sp. and Fasciola sp. Size varied between 40 μm and 200 μm for eggs and between 100 μm and 600 μm for the larvae assessed. Densities of environmental forms of the helminths were also higher during the short rainy season (176 ± 77 agents/L) than during the short dry season (117 ± 49 agents/L). These results show that muddy soils could contribute to the contamination of wells and springs and should be considered in epidemiological studies of intestinal parasites.
Topics: Animals; Cameroon; Feces; Parasites; Parasitic Diseases; Soil; Water; Water Supply
PubMed: 30942781
DOI: 10.2166/wh.2018.159 -
Protist May 2012Diplomonads, retortamonads, and "Carpediemonas-like" organisms (CLOs) are a monophyletic group of protists that are microaerophilic/anaerobic and lack typical...
Diplomonads, retortamonads, and "Carpediemonas-like" organisms (CLOs) are a monophyletic group of protists that are microaerophilic/anaerobic and lack typical mitochondria. Most diplomonads and retortamonads are parasites, and the pathogen Giardia intestinalis is known to possess reduced mitochondrion-related organelles (mitosomes) that do not synthesize ATP. By contrast, free-living CLOs have larger organelles that superficially resemble some hydrogenosomes, organelles that in other protists are known to synthesize ATP anaerobically. This group represents an excellent system for studying the evolution of parasitism and anaerobic, mitochondrion-related organelles. Understanding these evolutionary transitions requires a well-resolved phylogeny of diplomonads, retortamonads and CLOs. Unfortunately, until now the deep relationships amongst these taxa were unresolved due to limited data for almost all of the CLO lineages. To address this, we assembled a dataset of up to six protein-coding genes that includes representatives from all six CLO lineages, and complements existing rRNA datasets. Multigene phylogenetic analyses place CLOs as well as the retortamonad Chilomastix as a paraphyletic basal assemblage to the lineage comprising diplomonads and the retortamonad Retortamonas. In particular, the CLO Dysnectes was shown to be the closest relative of the diplomonads + Retortamonas clade, with strong support. This phylogeny is consistent with a drastic degeneration of mitochondrion-related organelles during the evolution from a free-living organism resembling extant CLOs to a probable parasite/commensal common ancestor of diplomonads and Retortamonas.
Topics: Animals; Anura; Diplomonadida; Evolution, Molecular; Molecular Sequence Data; Phylogeny; Protozoan Proteins; Retortamonadidae
PubMed: 22364773
DOI: 10.1016/j.protis.2011.12.007 -
Journal of the Egyptian Society of... Aug 2007A total of 350 stool samples from 196 males and 154 female children and neonatus admitted in Ibn-Sina hospital, Sirt, were examined from June 2001 to May 2002, to...
A total of 350 stool samples from 196 males and 154 female children and neonatus admitted in Ibn-Sina hospital, Sirt, were examined from June 2001 to May 2002, to determine the prevalence of intestinal parasites. Intestinal parasitic infections were identified in 196 (56%) of children and neonates. No intestinal helminthic parasites were detected but 13 intestinal protozoan parasites were detected. The most prevalent protozoan was Entamoeba histolytica /E. dispar (36.57%); Blastocystis hominis (12.57%), Giardia lamblia (10.29%), Isospora belli (3.14%) and Balantidium coli (0.86%), the latter was detected in non-Libyan children. The non-pathogenic ones were Entamoeba coli (15.14%), Endolimax nana (13.71%), Entamoeba hartmanni (4.29%), Chilomastix mesnilli (4.29%), Retortamonas intestinalis (3.43%), Dientamoeba fragilis (2%), Iodamoeba butschlii (0.86%) and Trichomonas hominis (0.86%). The result showed a significant difference exists between the prevalence of pathogenic and non-pathogenic protozoan parasites (P < 0.05). High prevalence of E. histolytica/ E. dispar followed by E. coli, E. nana, B. hominis and G. lamblia in both sexes of children, while the prevalence of other intestinal parasites were low in both sexes, significantly different existed in the prevalence of intestinal parasites between males and females children (t = 24.68; P < 0.05). Age groups had no effect on the prevalence of intestinal parasites (F = 0.66; P < 0.05). Significant differences existed in the prevalence between single and multiple infections with pathogenic protozoa. The socio-economic status of children parents revealed that high prevalence in children from medium socio-economic status. The family size had no significant effect on the prevalence of the intestinal parasites.
Topics: Adolescent; Age Factors; Animals; Child; Child, Preschool; Feces; Female; Helminthiasis; Humans; Infant; Infant, Newborn; Intestinal Diseases, Parasitic; Libya; Male; Prevalence; Protozoan Infections; Sex Factors; Social Class; Socioeconomic Factors
PubMed: 17985574
DOI: No ID Found -
American Journal of Primatology Feb 2004On the Indonesian island of Sulawesi, nonhuman primate pets come into frequent contact with humans, presenting the possibility of zoonotic and anthropozoonotic disease... (Comparative Study)
Comparative Study
On the Indonesian island of Sulawesi, nonhuman primate pets come into frequent contact with humans, presenting the possibility of zoonotic and anthropozoonotic disease transmission. We collected fecal samples from 88 pet macaques representing six of the seven macaque species currently recognized as endemic to Sulawesi (Macaca nigra, M. nigrescens, M. hecki, M. tonkeana, M. maura, and M. ochreata) as well as two non-endemic species (M. fascicularis and M. nemestrina) in order to determine the prevalence of intestinal parasitic infection in this population. Seven taxa of intestinal protozoa (Blastocystis hominis, Iodamoeba bütschlii, Entamoeba coli, Entamoeba hartmanni, Chilomastrix mesnili, Endolimax nana, and Retortamonas intestinalis) and three taxa of nematodes (hookworm, Trichuris spp., and Ascaris spp.) were detected. The overall parasitization rate was 59.1%. Commensal organisms predominated in this population. Parasitization was not statistically correlated with macaque age group, sex, species, or location, or with the owner's level of education. These findings are discussed in the context of primate pet ownership practices in Sulawesi.
Topics: Animals; Animals, Domestic; Cross-Sectional Studies; Disease Vectors; Feces; Indonesia; Intestines; Macaca; Monkey Diseases; Nematode Infections
PubMed: 14983465
DOI: 10.1002/ajp.20008 -
Parassitologia 1988A parasitological study was carried out on 381 apparently healthy subjects from Camiri, Boyuibe, Gutierrez. Intestinal parasites and non-pathogenic protozoa were present...
A parasitological study was carried out on 381 apparently healthy subjects from Camiri, Boyuibe, Gutierrez. Intestinal parasites and non-pathogenic protozoa were present in 78.7% of the population sampled; multiple infections were observed in 67.7% of the parasitized individuals. The protozoon most commonly found was Entamoeba coli (in 40.7% of specimens), followed by Giardia intestinalis (30.7%), Iodamoeba bütschlii (10%), Chilomastix mesnili (8.7%). Other protozoon parasites also present were Enteromonas hominis (3.4%), Retortamonas intestinalis (2.4%), Cryptosporidium (2.1%), Endolimax nana (2.1%), Balantidium coli (1.8%) and Pentatrichomonas hominis (0.8%). The helminths observed were hookworms (28.6%), Trichuris trichiura (19.7%), Ascaris lumbricoides (9.7%), Hymenolepis nana (8.7%), Trichostrongylus (5.5%), Strongyloides stercoralis (1.8%), Taenia (5 cases) and Enterobius (6 cases). Prevalence for nematodes is probably underestimated in the 3-9 years age group because of a mebendazole treatment given 5 weeks before the survey, under a Program of P D C of the Ministry of Health. The sample from Camiri was found to be the most parasitized (84.1%). An extraordinarily high infection rate was found in two urban institutions, as well as in Itanambicua, a rural community close to Camiri. No significant differences were observed in parasitic prevalence between rural and urban environments. Exposure to contamination with human and animal faeces, overcrowding and poor sanitation habits are some of the factors responsible for the parasitic situation evidenced.
Topics: Adolescent; Adult; Bolivia; Child; Child, Preschool; Feces; Female; Humans; Infant; Intestinal Diseases, Parasitic; Male
PubMed: 3271990
DOI: No ID Found -
Journal of Morphology May 1948
Topics: Animals; Male; Retortamonadidae; Vertebrates
PubMed: 18915636
DOI: 10.1002/jmor.1050820303 -
Turkiye Parazitolojii Dergisi 2009This study was carried out on school children in primary schools in the Van province in order to investigate the distribution of intestinal parasitoses. The study was...
This study was carried out on school children in primary schools in the Van province in order to investigate the distribution of intestinal parasitoses. The study was conducted in 7-15 year-old primary schoolchildren during the years from 2003-2005. Stool specimens were taken from 2975 school children; of these, 1289 were female and 1686, male. The specimens were examined by native-Lugol, flotation and trichrome staining methods in the parasitology laboratory of the Health Research and Training Hospital, Yüzüncü Yil University. One or more parasite species were detected in 1916 (64.4%) stool specimens of 2975 children. Parasitosis was detected in 65.7% of females and in 63.4% of males. Blastocystis hominis, Giardia intestinalis, Entamoeba coli, Iodamoeba butschlii, Chilomastix mesnili, Endolimax nana, Entamoeba histolytica/Entamoeba dispar, Entamoeba hartmanni, Enteromonas hominis, Retortamonas intestinalis, Dientamoeba fragilis, Trichomonas hominis, Ascaris lumbricoides, Hymenolepis nana, Trichuris trichiura, Enterobius vermicularis and Fasciola hepatica were detected in 27.4%, 16.4%, 14.9%, 3.9%, 2.9%, 1.8%, 1.1%, 0.6%, 0.5%, 0.5%, 0.4%, 0.2%, 7.5%, 5.3%, 0.7%, 0.6% and 0.03% of the specimens, respectively. In conclusion, there are still high rates of intestinal parasitoses in school children in this region because care is not taken in hygiene and the socioeconomic status is low in the Van province.
Topics: Adolescent; Child; Feces; Female; Humans; Hygiene; Intestinal Diseases, Parasitic; Male; Socioeconomic Factors; Turkey
PubMed: 20101580
DOI: No ID Found