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Biotechnology Advances Nov 2022Rhodopseudomonas palustris is an attractive option for biotechnical applications and industrial engineering due to its metabolic versatility and its ability to... (Review)
Review
Rhodopseudomonas palustris is an attractive option for biotechnical applications and industrial engineering due to its metabolic versatility and its ability to catabolize a wide variety of feedstocks and convert them to several high-value products. Given its adaptable metabolism, R. palustris has been studied and applied in an extensive variety of applications such as examining metabolic tradeoffs for environmental perturbations, biodegradation of aromatic compounds, environmental remediation, biofuel production, agricultural biostimulation, and bioelectricity production. This review provides a holistic summary of the commercial applications for R. palustris as a biotechnology chassis and suggests future perspectives for research and engineering.
Topics: Biodegradation, Environmental; Biofuels; Biotechnology; Rhodopseudomonas
PubMed: 35680002
DOI: 10.1016/j.biotechadv.2022.108001 -
Trends in Microbiology Mar 2022
Topics: Rhodopseudomonas
PubMed: 34933806
DOI: 10.1016/j.tim.2021.12.001 -
Annales de Microbiologie 1983The photosynthetic apparatus of the facultative phototrophic bacterium Rhodopseudomonas capsulata contains three bacteriochlorophyll-carotenoid-protein complexes: the... (Review)
Review
The photosynthetic apparatus of the facultative phototrophic bacterium Rhodopseudomonas capsulata contains three bacteriochlorophyll-carotenoid-protein complexes: the reaction center and the light-harvesting (LH) antenna complexes LHI (B870) and LHII (B800--850). In contrast to green anoxygenic phototrophic bacteria and the oxygenic cyanobacteria, the light-harvesting complexes of Rhodospirillaceae and Chromatiaceae are integral membrane particles. Variations in light fluxes induce membrane differentiation mainly expressed as variations in the size of the photosynthetic unit and in the area of intracytoplasmic membrane per cell. The B800--850 complex is the variable part of the photosynthetic apparatus. Synthesis of bacteriochlorophyll and of the polypeptides of the pigment complexes was found to be strongly coordinated. The synthesis of these polypeptides was followed immediately by the assembly of the complexes in the membrane. Bacteriochlorophyll or a signal substance triggered by bacteriochlorophyll synthesis regulated the synthesis of these polypeptides at the level of translation. The pigment-binding subunits of the B800--850 complex form oligomeric structures which interact with subunit H of the reaction center. A model of the topographical relationships of the pigment complexes is discussed.
Topics: Bacterial Proteins; Bacteriochlorophylls; Chlorophyll; Intracellular Membranes; Kinetics; Levulinic Acids; Light; Light-Harvesting Protein Complexes; Macromolecular Substances; Models, Biological; Photosynthesis; Photosynthetic Reaction Center Complex Proteins; Phycobilisomes; Plant Proteins; Protein Biosynthesis; Rhodopseudomonas
PubMed: 6357026
DOI: 10.1016/s0769-2609(83)80102-1 -
International Journal of Systematic and... Sep 2017A strain of anoxygenic phototrophic bacteria isolated from paddy soil (designated strain TUT3615T) was studied taxonomically in comparison with Rhodopseudomonasstrain...
A strain of anoxygenic phototrophic bacteria isolated from paddy soil (designated strain TUT3615T) was studied taxonomically in comparison with Rhodopseudomonasstrain ATCC 17005 as its nearest phylogenetic relative. Strains TUT3615T and ATCC 17005 had budding rod-shaped cells and showed in vivo absorption maxima at 804 and 860 nm in the near infrared region, indicating the presence of bacteriochlorophyll a. The intracytoplasmic membrane system was of the lamellar type parallel to the cytoplasmic membrane. 16S rRNA gene sequence comparisons showed that strains TUT3615T and ATCC 17005 had a 99.7 % level of similarity to one another and were closest to Rhodopseudomonas palustris ATCC 17001T (98.6 % similarity) among the established species of the genus Rhodopseudomonas. Genomic DNA-DNA hybridization studies revealed that strains TUT3615T and ATCC 17005 had an average similarity level of 65 % to one another and of less than 40 % to the available type strains of Rhodopseudomonas species. Results of phenotypic studies showed that strains TUT3615T and ATCC 17005 could be differentiated from one another and from any previously described species of Rhodopseudomonas. The G+C contents of the genomic DNA of strain TUT3615T and ATCC 17005 were 66.3 and 66.5 mol%, respectively. Based on these data, we propose the name Rhodopseudomonas telluris sp. nov. for strain TUT3615T. The type strain is TUT3615T (=KCTC 23279T=NBRC 107609T). We suspend a proposal to reclassify strain ATCC 17005 as a novel species or subspecies until a genome-wide analysis provides more definite information on its taxonomic position.
Topics: Bacterial Typing Techniques; Bacteriochlorophyll A; Base Composition; DNA, Bacterial; Fatty Acids; Japan; Nucleic Acid Hybridization; Oryza; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Rhodopseudomonas; Sequence Analysis, DNA; Soil Microbiology; Ubiquinone
PubMed: 28875892
DOI: 10.1099/ijsem.0.002120 -
Biochimica Et Biophysica Acta Feb 1962
Topics: Cytochromes; Rhodobacter sphaeroides; Rhodopseudomonas
PubMed: 14482254
DOI: 10.1016/0006-3002(62)91133-2 -
Applied and Environmental Microbiology May 2020The purple nonsulfur phototrophic bacterium strain CGA009 uses the three-carbon dicarboxylic acid malonate as the sole carbon source under phototrophic conditions....
The purple nonsulfur phototrophic bacterium strain CGA009 uses the three-carbon dicarboxylic acid malonate as the sole carbon source under phototrophic conditions. However, this bacterium grows extremely slowly on this compound and does not have operons for the two pathways for malonate degradation that have been detected in other bacteria. Many bacteria grow on a spectrum of carbon sources, some of which are classified as poor growth substrates because they support low growth rates. This trait is rarely addressed in the literature, but slow growth is potentially useful in biotechnological applications where it is imperative for bacteria to divert cellular resources to value-added products rather than to growth. This prompted us to explore the genetic and physiological basis for the slow growth of with malonate as a carbon source. There are two unlinked genes annotated as encoding a malonyl coenzyme A (malonyl-CoA) synthetase (MatB) and a malonyl-CoA decarboxylase (MatA) in the genome of , which we verified as having the predicted functions. Additionally, two tripartite ATP-independent periplasmic transporters (TRAP systems) encoded by to and to were needed for optimal growth on malonate. Most of these genes were expressed constitutively during growth on several carbon sources, including malonate. Our data indicate that uses a piecemeal approach to growing on malonate. The data also raise the possibility that this bacterium will evolve to use malonate efficiently if confronted with an appropriate selection pressure. There is interest in understanding how bacteria metabolize malonate because this three-carbon dicarboxylic acid can serve as a building block in bioengineering applications to generate useful compounds that have an odd number of carbons. We found that the phototrophic bacterium grows extremely slowly on malonate. We identified two enzymes and two TRAP transporters involved in the uptake and metabolism of malonate, but some of these elements are apparently not very efficient. cells growing with malonate have the potential to be excellent biocatalysts, because cells would be able to divert cellular resources to the production of value-added compounds instead of using them to support rapid growth. In addition, our results suggest that is a candidate for directed evolution studies to improve growth on malonate and to observe the kinds of genetic adaptations that occur to make a metabolic pathway operate more efficiently.
Topics: Biodegradation, Environmental; Biological Transport; Gene Expression Regulation, Bacterial; Malonates; Metabolic Networks and Pathways; Rhodopseudomonas
PubMed: 32220835
DOI: 10.1128/AEM.00631-20 -
Journal of Biotechnology Nov 2020Carotenoids (CD) are biological pigments produced for commercial purposes. Therefore, it is necessary to study and determine the optimal conditions for increased CD...
Carotenoids (CD) are biological pigments produced for commercial purposes. Therefore, it is necessary to study and determine the optimal conditions for increased CD production. There is no consensus in the literature about the conditions that increase CD production. Some authors stated that CD will be preferentially produced at low light intensities, at this adverse condition, microorganism will increase CD production as a survival response mechanism to get more energy. Other authors have mentioned that CD concentrations increase as the light intensity supplied increases, to avoid the overexposure of light and in turn photo-inhibition. Additionally, to increase the specific CD production is also necessary to increase the amount of biomass. In this work, the ammonium concentration (high (HAC) and low (LAC)) and the low light (LL) intensity effect on the CD production was evaluated. Data showed that a high CD-specific concentration of 8.8 mg/g was obtained by using R. palustris ATCC 17001 under HAC and LL intensity. CD production was similar at HAC and LAC, suggesting that the light intensity has a greater effect on the specific CD concentration than the nitrogen concentration. In general, the results showed a low biomass production compared to the literature, with high CD synthesis.
Topics: Ammonium Compounds; Biomass; Carotenoids; Kinetics; Light; Rhodopseudomonas
PubMed: 32827602
DOI: 10.1016/j.jbiotec.2020.08.007 -
Journal of Industrial Microbiology &... May 2016Squalene is a strong antioxidant used extensively in the food, cosmetic and medicine industries. Rhodopseudomonas palustris TIE-1 was used as the host because of its...
Squalene is a strong antioxidant used extensively in the food, cosmetic and medicine industries. Rhodopseudomonas palustris TIE-1 was used as the host because of its ability to grow photosynthetically using solar energy and carbon dioxide from atmosphere. The deletion of the shc gene resulted in a squalene production of 3.8 mg/g DCW, which was 27-times higher than that in the wild type strain. For constructing a substrate channel to elevate the conversion efficiency, we tried to fuse crtE gene with hpnD gene. By fusing the two genes, squalene content was increased to 12.6 mg/g DCW, which was 27.4 % higher than that resulted from the co-expression method. At last, the titer of squalene reached 15.8 mg/g DCW by co-expressing the dxs gene, corresponding to 112-fold increase relative to that for wild-type strain. This study provided novel strategies for improving squalene yield and demonstrated the potential of producing squalene by Rhodopseudomonas palustris.
Topics: Carbon Dioxide; Gene Deletion; Genes, Bacterial; Metabolic Engineering; Photosynthesis; Rhodopseudomonas; Squalene
PubMed: 26886756
DOI: 10.1007/s10295-016-1745-7 -
Archives of Microbiology Nov 1975Thirty-three strains of Rhodopseudomonas capsulata have been studied in order to develop a more comprehensive characterization of the species. On the basis of...
Thirty-three strains of Rhodopseudomonas capsulata have been studied in order to develop a more comprehensive characterization of the species. On the basis of morphological, nutritional, physiological and other properties, the characteristics of an "ideal biotype" have been defined, which can be used to distinguish Rps. capsulata from similar purple bacteria. In this connection, two properties of Rps. capsulata are of particular note: a) sensitivity to penicillin G is 10(3)-10(5) times greater than that shown by closely related species, and b) all strains examined are susceptible to lysis by one or more strains of host species-specific virulent bacteriophages. It appears that members of the species Rps. capsulata form a stringent taxonomic grouping.
Topics: Anaerobiosis; Bacteriochlorophylls; Bacteriolysis; Bacteriophages; Carbohydrate Metabolism; Carbon; Carotenoids; Hydrogen; Microscopy, Phase-Contrast; Mutation; Nitrogen Fixation; Penicillin G; Penicillin Resistance; Photosynthesis; Rhodopseudomonas; Spheroplasts; Temperature; Vitamins
PubMed: 1103769
DOI: 10.1007/BF00447139 -
Archiv Fur Mikrobiologie 1971
Topics: Agglutination; Antigen-Antibody Reactions; Antigens; Bacteriophages; Carotenoids; Chlorophyll; Cross Reactions; Culture Media; Immune Sera; Lipopolysaccharides; Rhodopseudomonas; Spectrophotometry
PubMed: 5098715
DOI: 10.1007/BF00412272