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Scientific American Feb 1962
Topics: Genetics; Humans; RNA; RNA, Messenger
PubMed: 14450186
DOI: 10.1038/scientificamerican0262-41 -
Journal of Separation Science May 2016The isolation and purification of ribonucleic acid have attracted attention recently for the understanding of the functions in detail because of the necessity for the...
The isolation and purification of ribonucleic acid have attracted attention recently for the understanding of the functions in detail because of the necessity for the treatment of genetic diseases. In this study, guanine-incorporated polymeric cryogels were developed to obtain highly purified ribonucleic acid. The satisfactory purification performance was achieved with the guanine-incorporated poly (2-hydroxyethyl methacrylate-guanine methacrylate) cryogels. The most crucial advantages to use guanine as a functional monomer are to obtain a real natural interaction between guanine on the polymeric material and cytosine on the ribonucleic acid. Moreover, using cryogel with a highly porous structure and high swelling ratio provide advantages of getting more water within the structure to get more analyte to interact. The characterization of cryogels has proved the success of the synthesis and the perfect natural interaction to be taken place between the ligand (guanine methacrylate) and the cytosine in the ribonucleic acid molecules. Although the pores within the structure of cryogels are small, they provide efficient and fast adsorption. The chromatographic separation performance was investigated for different conditions (pH, temperature etc.). The desorption ratio and reusability were also analyzed at the end of the five adsorption-desorption cycles with no significant changes.
Topics: Cryogels; Guanine; Methacrylates; Polymers; RNA
PubMed: 27004613
DOI: 10.1002/jssc.201600199 -
British Medical Bulletin Jan 1962
Topics: Bacteria; Humans; RNA
PubMed: 13873643
DOI: 10.1093/oxfordjournals.bmb.a069925 -
Biochemical Society Transactions Dec 2015tRNAs are fundamental components of translation and emerging evidence places them more centrally in various other cellular processes. However, rather than being... (Review)
Review
tRNAs are fundamental components of translation and emerging evidence places them more centrally in various other cellular processes. However, rather than being uniformly conserved, tRNA abundance is instead highly variable and adaptable. The amount of tRNA genes greatly differs among species. Moreover, even within the same genome, tRNA abundance shapes the proteome in a tissue- and cell-specific manner and is dynamically regulated in response to stress. Here, we review approaches for identification and quantification of tRNAs and their functional integrity. We discuss the resolution of each method and highlight new approaches with cell-wide resolution based on deep-sequencing technologies.
Topics: Anticodon; Base Sequence; Chromatography, High Pressure Liquid; Electrophoresis, Gel, Two-Dimensional; High-Throughput Nucleotide Sequencing; Nucleic Acid Conformation; Oligonucleotide Array Sequence Analysis; RNA; RNA, Transfer; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 26614663
DOI: 10.1042/BST20150158 -
Survey of Biological Progress 1962
Topics: Communicable Diseases; RNA; Viruses
PubMed: 13894427
DOI: No ID Found -
Biochimica Et Biophysica Acta May 1959
Topics: Amino Acids; Chemical Fractionation; RNA; RNA, Transfer
PubMed: 13651227
DOI: 10.1016/0006-3002(59)90541-4 -
Proceedings of the National Academy of... Oct 1961
Topics: RNA
PubMed: 14480083
DOI: 10.1073/pnas.47.10.1689 -
Nature Nov 1959
Topics: Animals; Brain; Brain Diseases; Communicable Diseases; Encephalitis Viruses, Tick-Borne; Mice; RNA; Virus Diseases
PubMed: 13832519
DOI: 10.1038/1841581a0 -
Biochimica Et Biophysica Acta Jul 1960
Topics: Bacteria; Biochemical Phenomena; Enterobacter aerogenes; Physiological Phenomena; RNA
PubMed: 13728193
DOI: 10.1016/0006-3002(60)90757-5 -
Biochemical and Biophysical Research... Jan 1961
Topics: RNA
PubMed: 13766061
DOI: 10.1016/0006-291x(61)90246-7