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Minerva Chirurgica20 patients have been treated, 10 with ribostamycin sulphate, 5 with gentamycin and 5 with sodium cephalotin. Patients were selected at random from those who had... (Clinical Trial)
Clinical Trial Comparative Study
20 patients have been treated, 10 with ribostamycin sulphate, 5 with gentamycin and 5 with sodium cephalotin. Patients were selected at random from those who had undergone abdominal, thoracic or cervical operations. Doses were 1000-2000 mg i.m. for ribostamycin, 4-8 g i.v. for cephalotin, and 160-240 mg i.m. for gentamycin. Mean treatment time was 12.5 days. Indices of therapeutic activity, parameters of liver and renal function and otovestibular function are indicated. The therapeutic activity of ribostamycin was very good and no side-effects were observed.
Topics: Adult; Aged; Anti-Bacterial Agents; Bacterial Infections; Cephalothin; Female; Gentamicins; Humans; Male; Middle Aged; Postoperative Complications; Ribostamycin; Surgical Procedures, Operative
PubMed: 7290416
DOI: No ID Found -
Antimicrobial Agents and Chemotherapy Jun 1974A new aminoglycoside antibiotic was isolated from the fermentation broths of two strains of Bacillus species. The antibiotic is active against gram-positive and some...
A new aminoglycoside antibiotic was isolated from the fermentation broths of two strains of Bacillus species. The antibiotic is active against gram-positive and some gram-negative bacteria, and its antimicrobial spectrum is similar to that of ribostamycin. The chemical structure was determined to be 5-beta-d-xylofuranosylneamine, which is identical to the deacylated product obtained from butirosin A.
Topics: Acylation; Aminoglycosides; Anti-Bacterial Agents; Bacillus; Butirosin Sulfate; Fermentation; Framycetin; Hydrolysis
PubMed: 15825408
DOI: 10.1128/AAC.5.6.578 -
The Journal of Antibiotics Oct 2000Fourteen different aminoglycoside antibiotics (AGs) were challenged with aminoglycoside acetyltransferases (AACs) of actinomycete origin in order to examine their...
Fourteen different aminoglycoside antibiotics (AGs) were challenged with aminoglycoside acetyltransferases (AACs) of actinomycete origin in order to examine their 'double stage activity' that is arbitrarily defined as antibiotic activity retainable after enzymatic modification. In kanamycin (KM)-group AGs tested [KM, dibekacin (DKB), amikacin and arbekacin (ABK)], ABK retained activity after acetylations by AAC(3), AAC(2') and AAC(6'). DKB also retained a weak activity after acetylation by AAC(2'). In gentamicin (GM)-group AGs tested [GM, micronomicin, sisomicin (SISO), netilmicin (NTL) and isepamicin], GM, SISO and NTL retained activites after acetylation by AAC(2'). In neomycin (NM)-group AGs tested [ribostamycin, NM, paromomycin], NM retained activity after acetylation by AAC(6') and AAC(2'). None of astromicin (ASTM)-group AGs tested (ASTM and istamycin B) retained activity after acetylation by AAC(2') and AAC(6'). The activities of acetylated ABK derivatives by AAC(3) and AAC(2') were distinctively high, compared to the others. Streptomyces lividans TK21 containing the cloned aac genes were markedly sensitive to AGs that retained activities after acetylation, indicating the substantial effect of 'double stage activity'.
Topics: Acetylation; Acetyltransferases; Actinomycetales; Aminoglycosides; Anti-Bacterial Agents; Bacillus subtilis; Carbohydrate Sequence; Chromatography, Thin Layer; Drug Resistance, Microbial; Microbial Sensitivity Tests; Molecular Sequence Data; Streptomyces
PubMed: 11132963
DOI: 10.7164/antibiotics.53.1168 -
The Journal of Antibiotics Oct 1983Antibiotic-resistance genes were shotgun cloned from antibiotic-producing Streptomyces sp. using pock-forming plasmids (pSF689 and pSF765), as cloning vectors.... (Comparative Study)
Comparative Study
Antibiotic-resistance genes were shotgun cloned from antibiotic-producing Streptomyces sp. using pock-forming plasmids (pSF689 and pSF765), as cloning vectors. Streptomyces chartreusis SF1623 and S. lividans 66 were used as host strains. The ribostamycin (RSM) resistance gene was cloned from S. ribosidificus SF733 DNA (on a 2.3 Md PstI fragment) into both S. chartreusis SF1623 and S. lividans 66, using pSF689 as vector. Kanamycin (KM), novobiocin (NB), destomycin (DM) and racemomycin (RM) resistance genes were cloned from S. kanamyceticus M1164, S. spheroides M1469, S. rimofaciens M1470 and S. lavendulae A249 genomic DNA into S. lividans 66, using pSF765 as vector. Furthermore two types of KM resistance determinants derived from S. kanamyceticus M1164 were cloned using S. lividans 66, the pSF689 vector. The RSM resistance gene showed no homology to plasmid pSF733 of S. ribosidificus SF733, but hybridized to PstI or BclI digested total DNA of S. ribosidificus SF733.
Topics: Anti-Bacterial Agents; Cloning, Molecular; Drug Resistance, Microbial; Genes, Bacterial; Genetic Vectors; Plasmids; Species Specificity; Streptomyces; Structure-Activity Relationship
PubMed: 6315667
DOI: 10.7164/antibiotics.36.1305 -
Giornale Di Clinica Medica 1983
Topics: Adult; Aged; Anti-Bacterial Agents; Bronchitis; Drug Evaluation; Female; Humans; Male; Middle Aged; Pneumonia; Ribostamycin
PubMed: 6642111
DOI: No ID Found -
Immunitat Und Infektion Apr 1981Nephrotoxicity, pharmacokinetics, and therapeutic efficacy of ribostamycin were evaluated in rats. Measures of nephrotoxicity were urinary excretion of tubular cells and... (Comparative Study)
Comparative Study
Nephrotoxicity, pharmacokinetics, and therapeutic efficacy of ribostamycin were evaluated in rats. Measures of nephrotoxicity were urinary excretion of tubular cells and malate dehydrogenase. The i.v. injections of ribostamycin lead to the same tubular cell excretion values as the i.m. injections. Parallel administration of D-glucaro-delta-lactam reduced the nephrotoxic effect of ribostamycin significantly. The lowest dose which leads to a significantly increased loss of tubular cells was 20 mg/kg/d and was distinctly above that of gentamicin, indicating a lower nephrotoxicity. This corresponded to lower renal ribostamycin concentrations after a single dose administration as well as after 9 times repeated dosing. In chemotherapy of the acute pyelonephritis of the rat ribostamycin was inferior to gentamicin at dosages of 2.5 or 5 mg/kg.
Topics: Animals; Anti-Bacterial Agents; Female; Gentamicins; Glucaric Acid; Half-Life; Kidney; Lactams; Pyelonephritis; Rats; Ribostamycin; Tissue Distribution
PubMed: 7228116
DOI: No ID Found -
The Journal of Antibiotics Dec 1973
Topics: Carbon Isotopes; Magnetic Resonance Spectroscopy
PubMed: 4792382
DOI: 10.7164/antibiotics.26.717 -
International Journal of Clinical... Mar 1983The vestibular toxicities of dibekacin, ribostamycin, gentamicin, and streptomycin were compared in guinea pigs. The animals were given the drugs for 21 days, examined... (Comparative Study)
Comparative Study
The vestibular toxicities of dibekacin, ribostamycin, gentamicin, and streptomycin were compared in guinea pigs. The animals were given the drugs for 21 days, examined for functional disturbances by electronystagmography (ENG), and then killed to observe morphologic changes in the vestibular sensory hairs on scanning electron microscopy (SEM). The degree of functional impairment was rated according to the duration of the postrotatory nystagmus and classified into three categories: normal, slight impairment, and severe impairment. The results of the SEM examination were similarly classified into three categories by severity of the degenerative changes in the vestibular sensory hairs, i. e., bending, fusion, ballooning, giant hair formation, or disappearance. Under these experimental conditions, the results of ENG and SEM observations showed good correlation and indicated that the vestibular toxicity was highest in the streptomycin-treated animals and lowest in the dibekacin- or ribostamycin-treated animals.
Topics: Aminoglycosides; Animals; Anti-Bacterial Agents; Dibekacin; Electronystagmography; Guinea Pigs; Kanamycin; Microscopy, Electron, Scanning; Ribostamycin; Saccule and Utricle; Vestibule, Labyrinth
PubMed: 6602100
DOI: No ID Found -
The Japanese Journal of Antibiotics Apr 1977Neuromuscular blocking properties of tobramycin (Tobracin 60mg), dibekacin (Panmycin 100 mg) and ribostamycin (Vistamycin 1g) were studied in man during anesthesia and...
Neuromuscular blocking properties of tobramycin (Tobracin 60mg), dibekacin (Panmycin 100 mg) and ribostamycin (Vistamycin 1g) were studied in man during anesthesia and surgery by observing the effects of these antibiotics on muscle twitch tension. These antibiotics alone did not show any neuromuscular blocking action in the therapeutic doses. The neuromuscular blocking action of d-tubocurarine chloride was potentiated by the intravenous administration of 1g of ribostamycin. Tobramycin 60mg did not show remarkable effect, but dibekacin 100mg produced a slight potentiating effect on the action of d-tubocurarine. The enhancement of the action of d-tubocurarine was anatagonized by edrophonium and calcium. The action of succinylcholine chloride was not affected by tobramycin or ribostamycin, but it was potentiated transitorily by dibekacin. It is advisable to pay attention to possible respiratory depression when ribostamycin and dibekacin and used in combination with non-depolarizing muscle relaxants and/or anesthetics.
Topics: Anti-Bacterial Agents; Calcium; Dibekacin; Drug Synergism; Edrophonium; Humans; Kanamycin; Male; Neuromuscular Blocking Agents; Ribostamycin; Succinylcholine; Tobramycin; Tubocurarine
PubMed: 864858
DOI: No ID Found -
European Journal of Mass Spectrometry... Feb 2021Aminoglycosides are a class of broad-spectrum antibiotics with several clinical uses. Owing to the ototoxicity and nephrotoxicity of aminoglycosides, therapeutic drug...
Aminoglycosides are a class of broad-spectrum antibiotics with several clinical uses. Owing to the ototoxicity and nephrotoxicity of aminoglycosides, therapeutic drug monitoring is required. This study aimed to devise a high-throughput method for identification and quantitative determination of aminoglycoside antibiotics in human plasma samples using ultra-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry (UPLC-Q-ToF-MS). Plasma samples (100 µL) spiked with five aminoglycosides (streptomycin, spectinomycin, amikacin, kanamycin, and gentamycin) and an internal standard (ribostamycin) were diluted and centrifuged in aqueous formic acid and acetonitrile. The clear supernatant extract was evaporated and reconstituted in the mobile phase, of which 4 µL was subjected to UPLC-Q-ToF-MS. Prominent peaks were observed for the drugs within 3 min. The recoveries of five aminoglycosides from plasma samples were 92.6-120%. The regression equations showed excellent linearity (0.9999 ≥ r ≥ 0.9987) within the range of 1.0-100 µg/mL, and detection limits of 0.5-2.0 µg/mL. The coefficients of the intra- and inter-day variations for five drugs were less than 11.8%, while the accuracy of quantitation was in the range of 89-111%. In this study, a novel method was presented for identification and determination of aminoglycosides in human plasma samples using UPLC-Q-ToF-MS analysis. This method can be applied to high-throughput analysis used for clinical and environmental purposes.
Topics: Aminoglycosides; Anti-Bacterial Agents; Chromatography, High Pressure Liquid; Humans; Pharmaceutical Preparations; Tandem Mass Spectrometry
PubMed: 33745337
DOI: 10.1177/14690667211003196