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International Journal of Systematic and... Jun 2008A polyphasic study was carried out to clarify the taxonomic position of a pink-coloured, Gram-negative bacterium isolated from air in the Suwon region of Korea. The...
A polyphasic study was carried out to clarify the taxonomic position of a pink-coloured, Gram-negative bacterium isolated from air in the Suwon region of Korea. The novel strain, 5420S-30(T), grew in the temperature range of 5-35 degrees C (optimum 30 degrees C) and pH range of 5-9 (optimum pH 6-7). Growth did not occur in the presence of as little as 1 % (w/v) NaCl. It contained Q-10 as the predominant ubiquinone. The major fatty acids were C(18 : 1)omega7c, summed feature 3 (C(16 : 1)omega7c/iso-C(15 : 0) 2-OH) and C(16 : 0) (together representing 77.7 % of the total). The DNA G+C content was 72.1 mol%. The 16S rRNA gene sequence similarities between strain 5420S-30(T) and members of the genus Roseomonas were in the range 91.6-95.1 %, with the highest sequence similarities (95.1 and 94.5 %, respectively) to Roseomonas aquatica TR53(T) and Roseomonas gilardii ATCC 49956(T). On the basis of its phenotypic and genotypic characteristics, strain 5420S-30(T) represents a novel species of the genus Roseomonas, for which the name Roseomonas aerilata sp. nov. is proposed, with 5420S-30(T) (=KACC 12521(T) =DSM 19363(T)) as the type strain.
Topics: Air Microbiology; Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Genes, rRNA; Genotype; Korea; Methylobacteriaceae; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Species Specificity
PubMed: 18523199
DOI: 10.1099/ijs.0.65385-0 -
International Journal of Systematic and... Jan 2006An aerobic, Gram-negative bacterial strain, TH-G33T, was isolated from freshwater sediment of Taihu Lake in China. The taxonomy of strain TH-G33T was studied by using...
An aerobic, Gram-negative bacterial strain, TH-G33T, was isolated from freshwater sediment of Taihu Lake in China. The taxonomy of strain TH-G33T was studied by using phenotypic and phylogenetic methods. Cells of strain TH-G33T were coccoid rods or rods and formed colourless to pale-pink colonies on nutrient agar. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences showed that strain TH-G33T was related to Roseomonas mucosa (94.4 %), Roseomonas gilardii subsp. gilardii (94.1 %), Roseomonas gilardii subsp. rosea (94.8 %) and Roseomonas cervicalis (93.9 %). Cells contained ubiquinone 10 (Q-10) as the major quinone and the G+C content was 71.9 mol%. Thus, strain TH-G33T represents a novel species of the genus Roseomonas, for which the name Roseomonas lacus sp. nov. is proposed. The type strain is TH-G33T (= CGMCC 1.3617T = JCM 13283T).
Topics: Agar; Base Composition; China; Culture Media; Fresh Water; Geologic Sediments; Methylobacteriaceae; Molecular Sequence Data; RNA, Bacterial; RNA, Ribosomal, 16S; Species Specificity; Ubiquinone
PubMed: 16403861
DOI: 10.1099/ijs.0.63938-0 -
IDCases 2023rarely causes infection in humans. We report that a patient with underlying rheumatoid arthritis and diabetes developed wrist septic arthritis and osteomyelitis due to...
rarely causes infection in humans. We report that a patient with underlying rheumatoid arthritis and diabetes developed wrist septic arthritis and osteomyelitis due to after steroid joint injection. After antibiotic and surgical treatment, the condition of the patient improved. We reviewed previously reported cases of soft tissue, joint and bone infection related to to understand the characteristics of joint and bone infection of .
PubMed: 37234729
DOI: 10.1016/j.idcr.2023.e01792 -
Peritoneal Dialysis International :... Sep 2016
Topics: Anti-Bacterial Agents; Drug Therapy, Combination; Female; Follow-Up Studies; Gram-Negative Bacterial Infections; Humans; Infusions, Parenteral; Kidney Failure, Chronic; Methylobacteriaceae; Middle Aged; Peritoneal Dialysis, Continuous Ambulatory; Peritonitis; Rare Diseases; Severity of Illness Index; Treatment Outcome
PubMed: 27659935
DOI: 10.3747/pdi.2016.00103 -
Journal of Medical Microbiology Nov 2009The genus Roseomonas comprises groups of slow-growing, Gram-negative coccobacilli, which only infrequently cause infection in humans. When identified, they are...
The genus Roseomonas comprises groups of slow-growing, Gram-negative coccobacilli, which only infrequently cause infection in humans. When identified, they are associated with immunocompromised adults, often causing bacteraemia. Due to their rarity, members of this genus can be overlooked or misidentified using automated laboratory identification systems. We report on an immunocompetent adolescent patient who developed septic arthritis due to Roseomonas gilardii following surgery for a sports injury. The isolate was initially misidentified as Bordetella bronchiseptica using the Vitek 2 system, but confirmed as R. gilardii based on 16S rRNA gene sequencing. To the best of our knowledge, this is the first case of a healthy paediatric patient with septic arthritis due to R. gilardii.
Topics: Acetobacteraceae; Adolescent; Arthritis, Infectious; Female; Gram-Negative Bacterial Infections; Humans; Immunocompetence; Knee Joint; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 19574413
DOI: 10.1099/jmm.0.011106-0 -
Journal of Clinical Microbiology Dec 1993In the 1980s, a pink bacterium different from species of the genus Methylobacterium was implicated in human infection. Using biochemical tests and DNA hybridization, we...
In the 1980s, a pink bacterium different from species of the genus Methylobacterium was implicated in human infection. Using biochemical tests and DNA hybridization, we examined 42 strains of pink-pigmented, gram-negative bacteria that were not members of the genus Methylobacterium. The isolates included 6 strains each of CDC "pink coccoid" groups I, II, III, and IV; 10 isolates from Gilardi's "unnamed taxon"; and 8 blood isolates from ill, debilitated, or immunosuppressed patients. The DNA hybridization studies supported the creation of six genomospecies encompassing the 42 strains. Reactions for esculin hydrolysis, glycerol oxidation, and D-mannose oxidation enabled separation of genomospecies 1 through 4. These tests, as well as motility, nitrate reduction, citrate utilization, and oxidation of L-arabinose, D-galactose, and D-xylose, differentiated genomospecies 5 and 6 from each other and from genomospecies 1 through 4. These organisms were susceptible in vitro to the aminoglycosides, tetracycline, and imipenem and generally susceptible to the quinolones. We propose the new genus, Roseomonas, for these bacteria to include three named species, Roseomonas gilardii sp. nov., Roseomonas cervicalis sp. nov., and Roseomonas fauriae sp. nov., and three unnamed genomospecies.
Topics: Bacteremia; DNA, Bacterial; Drug Resistance, Microbial; Gram-Negative Bacteria; Gram-Negative Bacterial Infections; Humans; Nucleic Acid Hybridization; Pigmentation; Terminology as Topic
PubMed: 8308122
DOI: 10.1128/jcm.31.12.3275-3283.1993 -
International Journal of Systematic and... Oct 2006Strain TR53(T), a Gram-negative, non-motile, non-spore-forming and strictly aerobic coccobacillus, isolated from the drinking water distribution system of Seville,...
Strain TR53(T), a Gram-negative, non-motile, non-spore-forming and strictly aerobic coccobacillus, isolated from the drinking water distribution system of Seville, Spain, was subjected to polyphasic taxonomic analysis using a combination of phenotypic, genotypic and phylogenetic features. Phylogenetic analysis of 16S rRNA gene sequences showed that strain TR53(T) had highest similarity to members of the genus Roseomonas, with sequence similarity values between 95.7 % (to Roseomonas genomospecies 5 strain ATCC 49960) and 94.0 % (to Roseomonas gilardii subsp. rosea ATCC 49956(T)). On the basis of its phenotypic characteristics, 16S rRNA gene sequence data and DNA G+C content (68.6 mol%), strain TR53(T) represents a novel species of the genus Roseomonas, for which the name Roseomonas aquatica sp. nov. is proposed. The type strain of Roseomonas aquatica is TR53(T) (=CECT 7131(T)=JCM 13556(T)).
Topics: Bacterial Typing Techniques; Base Composition; DNA, Bacterial; DNA, Ribosomal; Fresh Water; Genes, rRNA; Methylobacteriaceae; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Spain; Water Supply
PubMed: 17012549
DOI: 10.1099/ijs.0.64379-0 -
American Journal of Clinical Pathology Aug 1997Roseomonas is a recently described genus of gram-negative coccobacilli formerly designated as "pink-coccoid" groups I through IV by the Centers for Disease Control and...
Roseomonas is a recently described genus of gram-negative coccobacilli formerly designated as "pink-coccoid" groups I through IV by the Centers for Disease Control and Prevention (Atlanta, Ga) because of the organism's characteristic pink colonies. Since 1991 we have isolated Roseomonas from eight patients; in seven from blood cultures and in one from a skin lesion. The seven blood isolates were from patients with clinically significant underlying diseases who had central venous catheters in place; the majority were associated with polymicrobial catheter infections. Additional characteristics of their infections are described. The eight isolates had originally been identified by us as Centers for Disease Control (CDC) pink-coccoid group III. These organisms were re-identified using the criteria of Rihs et al, and all isolates fit most closely with Roseomonas gilardii. Antibiotic profiles were fairly homogeneous showing susceptibility to many antibiotics, but uniform resistance to cefoxitin, ceftazidime, and piperacillin. Attempts to determine whether the isolates were the same strain by pulsed-field gel electrophoresis suggested that 3 of the isolates were similar. Random amplified polymorphic DNA analysis, however, demonstrated that each of the eight isolates was a unique strain.
Topics: Adult; Bacterial Typing Techniques; DNA, Bacterial; Electrophoresis, Gel, Pulsed-Field; Female; Gram-Negative Bacteria; Gram-Negative Bacterial Infections; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Polymerase Chain Reaction
PubMed: 9260763
DOI: 10.1093/ajcp/108.2.210 -
Journal of Clinical Microbiology Oct 1997
Topics: Bacteremia; Catheters, Indwelling; Gram-Negative Bacteria; Gram-Negative Bacterial Infections; Humans; Immunocompromised Host; Leukemia, Myeloid, Acute; Male; Microbial Sensitivity Tests; Middle Aged
PubMed: 9316945
DOI: 10.1128/jcm.35.10.2712-2712.1997 -
Clinical Infectious Diseases : An... Jun 2004This report analyzes 36 cases of bacteremia or catheter-related infection caused by Roseomonas species, a group of pink, slimy, waterborne, gram-negative coccobacilli....
This report analyzes 36 cases of bacteremia or catheter-related infection caused by Roseomonas species, a group of pink, slimy, waterborne, gram-negative coccobacilli. The causative species included the newly described Roseomonas mucosa (22 cases [61%]) and Roseomonas gilardii subspecies rosea (8 cases [22%]) and known species R. gilardii subspecies gilardii (5 cases [14%]) and Roseomonas genomospecies 4 (1 case [3%]). Twenty-nine (81%) of the cases were symptomatic, with fever being the most common symptom (in 27 [75%] of the cases). Twenty (56%) of the infections were monomicrobic. Six cases (17%) involved persistent catheter colonization, and 5 of these cases required removal of the catheter to clear the infection. All infections resolved, most with empirical antibiotic treatment. A summary of the antibiotic susceptibility pattern of these strains and other reported series show that Roseomonas species are consistently susceptible to amikacin and imipenem and frequently susceptible to ciprofloxacin and ticarcillin, but essentially nonsusceptible to ceftazidime and cefepime. This result may guide future therapy for infections due to Roseomonas species.
Topics: Adult; Aged; Bacteremia; Bacterial Typing Techniques; Catheters, Indwelling; Device Removal; Female; Gram-Negative Bacterial Infections; Humans; Male; Methylobacteriaceae; Middle Aged; Neoplasms; Retrospective Studies; Species Specificity
PubMed: 15156446
DOI: 10.1086/420824