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Leukemia & Lymphoma Apr 2005A 42-year-old male patient with a history of diffuse large B-cell non-Hodgkin's lymphoma (DLBCL) developed a central line-related bacteremia due to the presence of a...
A 42-year-old male patient with a history of diffuse large B-cell non-Hodgkin's lymphoma (DLBCL) developed a central line-related bacteremia due to the presence of a Gram-negative bacillus, which was difficult to identify conventionally. Sequencing of a partial region of the 16S rRNA gene identified the organism as Roseomonas mucosa with a homology score of 100% with 1003 bases called. Due to difficulties with the phenotypic identification of this genus, coupled with its emergence in line-related bacteremia in hematology patients with malignancy, Roseomonas spp. should be considered in cases of line-related infection in such patients with atypical Gram-negative organisms. Although several cases have been reported in the literature of line-related sepsis due to Roseomonas gilardii, only a few cases have been reported of Roseomonas mucosa infection in patients with hematological malignancy. This report highlights the benefits of the integration of a sequence-based typing approach in the identification of difficult-to-identify bacterial isolates employing partial regions of the 16S rRNA gene. Continued routine adoption of such techniques by clinical diagnostic laboratories may prove beneficial for the correct identification of blood-borne infections, as well as for the correct epidemiological characterization of unusual causal agents of bacteremia in immunocompromised individuals.
Topics: Adult; Bacteremia; Gram-Negative Bacterial Infections; Humans; Lymphoma, B-Cell; Lymphoma, Large B-Cell, Diffuse; Male; Methylobacteriaceae; RNA, Ribosomal, 16S; Remission Induction; Species Specificity
PubMed: 16019492
DOI: 10.1080/10428190400029908 -
Journal of Advanced Veterinary and... Mar 2024The objectives of this study were to determine the richness, abundance, and diversity of bacteria in stray dogs () infested by ticks in Comarca Lagunera, northern...
OBJECTIVE
The objectives of this study were to determine the richness, abundance, and diversity of bacteria in stray dogs () infested by ticks in Comarca Lagunera, northern Mexico, and to establish their pathogenic and or/zoonotic potential.
MATERIALS AND METHODS
Blood samples from 12 dogs were collected, and their deoxyribonucleic acid was extracted. The V3-V4 region of the 16S ribosomal ribunocleic acid gene was amplified by polymerase chain reaction. Next-generation sequencing (NGS) was performed on a MiSeq Illumina platform, and the data were analyzed using quantitative insights into microbial ecology.
RESULTS
The operational taxonomic units resulted in 23 phyla, 54 classes, 89 orders, 189 families, 586 genera, and 620 bacterial species; among them, 64 species and/or bacterial genera with pathogenic or zoonotic potential were identified, some of which have been reported in the literature as relevant to public health ( spp spp spp spp spp spp spp and ).
CONCLUSION
This research offers relevant information on the prevalence of tick-borne diseases as well as other potential zoonotic diseases in the blood of stray dogs parasitized by ticks in northern Mexico. New molecular biology and massive NGS techniques may play an important role in the study and documentation of bacterial profiles from animals in close proximity to humans.
PubMed: 38680790
DOI: 10.5455/javar.2024.k757 -
Enfermedades Infecciosas Y... May 2000
Topics: AIDS-Related Opportunistic Infections; Adult; Bone Marrow Diseases; Cameroon; Female; Gram-Negative Bacteria; Gram-Negative Bacterial Infections; Humans; Spain; Tuberculosis, Osteoarticular
PubMed: 10974778
DOI: No ID Found -
International Journal of Systematic and... Jan 2006Strain CP2CT was isolated from biological soil crusts in the Colorado Plateau, USA. The isolate was aerobic, facultatively fermentative, Gram-negative, non-motile and...
Strain CP2CT was isolated from biological soil crusts in the Colorado Plateau, USA. The isolate was aerobic, facultatively fermentative, Gram-negative, non-motile and red-pigmented (due to the presence of carotenoids), but did not contain bacteriochlorophyll a. The strain tested positive for catalase, oxidase and urease and was negative for lysine and ornithine decarboxylases and arginine dihydrolase. The major fatty acids present were C(18 : 1)omega7c and C(16 : 0). It had a high DNA G+C content of 75 mol%. Comparisons of 16S rRNA gene sequences identified bacteriochlorophyll a-producing strains of Paracraurococcus ruber (94.9 %), Craurococcus roseus (92.2 %) and Roseococcus thiosulfatophilus (92.3 %), as well as non-bacteriochlorophyll a-producing bacteria Muricoccus roseus (94.9 %), Roseomonas gilardii (94.2 %) and Roseomonas mucosa (93.8 %), as the bacteria most closely related to strain CP2CT. Phylogenetically, CP2CT was placed roughly equidistantly from the above organisms. Based on its phylogenetic placement and morphological and physiological characteristics, strain CP2CT is assigned to a new genus in the alpha-1 subgroup of the Proteobacteria, for which the name Belnapia gen. nov. is proposed. Strain CP2CT (= ATCC BAA-1043T = DSM 16746T) is proposed as the type strain of the type species of this genus, with the name Belnapia moabensis gen. nov., sp. nov.
Topics: Alphaproteobacteria; Bacterial Proteins; Base Composition; Catalase; Colorado; Fatty Acids; Fermentation; Molecular Sequence Data; Oxidoreductases; RNA, Bacterial; RNA, Ribosomal, 16S; Sequence Homology, Nucleic Acid; Soil Microbiology; Species Specificity; Urease
PubMed: 16403866
DOI: 10.1099/ijs.0.63764-0 -
Canadian Journal of Microbiology Apr 2004Four phenotypically similar bacterial strains isolated from fungal, plant, and human sources were identified as Azospirillum species. Strains RC1 and LOD4 were isolated... (Comparative Study)
Comparative Study
Four phenotypically similar bacterial strains isolated from fungal, plant, and human sources were identified as Azospirillum species. Strains RC1 and LOD4 were isolated from the mycelium of the apple root pathogen Rhizoctonia solani AG 5 and from the rhizosphere of wheat grown in apple orchard soil, respectively. Strains C610 and F4626 isolated from human wounds were previously misclassified as Roseomonas genomospecies 3 and 6. All four strains demonstrated close similarities in 16S rRNA gene sequences, having > or =97% identity to Azospirillum brasilense type strain ATCC 29145 and <90% identity to Roseomonas gilardii, the Roseomonas type strain. Extensive phenotypic similarities among the four strains included the ability of free-living cells to fix N2. Cells of strains RC1, LOD4, and C610 but not of strain F4626 could be induced to flocculate by incubation with 10 mmol.L-1 glycerol or fructose in medium containing 0.5 mmol.L-1 NO3-. Our results indicate a wide range of potential sources for Azospirillum spp. with the isolation of Azospirillum spp. from human wounds warranting further investigation.
Topics: Azospirillum; Culture Media; Humans; Plant Roots; Rhizoctonia; Sequence Analysis, DNA; Skin; Wounds and Injuries
PubMed: 15213753
DOI: 10.1139/w04-007