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Infection Control : IC May 1986
Review
Topics: Anti-Bacterial Agents; Arthritis, Infectious; Cross Infection; Drug Resistance, Microbial; Endocarditis, Bacterial; Enterobacteriaceae Infections; Humans; Infant, Newborn; Osteomyelitis; Pigments, Biological; Respiratory Tract Infections; Serratia marcescens; Surgical Wound Infection; Urinary Tract Infections
PubMed: 3519496
DOI: 10.1017/s0195941700064201 -
The New England Journal of Medicine Aug 1979
Topics: Penicillin Resistance; Penicillins; Serratia marcescens
PubMed: 379645
DOI: No ID Found -
British Medical Journal May 1977
Topics: Drug Resistance, Microbial; Enterobacteriaceae Infections; Serratia marcescens
PubMed: 324564
DOI: 10.1136/bmj.1.6070.1177-a -
Revista Chilena de Infectologia :... Jun 2010
Topics: Serratia marcescens
PubMed: 20737122
DOI: No ID Found -
The Journal of the Medical Society of... Jun 1970
Topics: Adult; Aged; Cross Infection; Enterobacteriaceae Infections; Female; Humans; Male; Middle Aged; Serratia marcescens
PubMed: 4913703
DOI: No ID Found -
Science (New York, N.Y.) Apr 1970
Topics: Humans; Laboratories; Serratia marcescens; Students
PubMed: 4906787
DOI: 10.1126/science.168.3927.64 -
Annals of Clinical Microbiology and... Mar 2017Serratia marcescens is a Gram-negative bacterium with proven resistance to multiple antibiotics and causative of catheter-associated infections. Bacterial colonization...
Serratia marcescens is a Gram-negative bacterium with proven resistance to multiple antibiotics and causative of catheter-associated infections. Bacterial colonization of catheters mainly involves the formation of biofilm. The objectives of this study were to explore the susceptibility of S. marcescens biofilms to high doses of common antibiotics and non-antimicrobial agents. Biofilms formed by a clinical isolate of S. marcescens were treated with ceftriaxone, kanamycin, gentamicin, and chloramphenicol at doses corresponding to 10, 100 and 1000 times their planktonic minimum inhibitory concentration. In addition, biofilms were also treated with chemical compounds such as polysorbate-80 and ursolic acid. S. marcescens demonstrated susceptibility to ceftriaxone, kanamycin, gentamicin, and chloramphenicol in its planktonic form, however, only chloramphenicol reduced both biofilm biomass and biofilm viability. Polysorbate-80 and ursolic acid had minimal to no effect on either planktonic and biofilm grown S. marcescens. Our results suggest that supratherapeutic doses of chloramphenicol can be used effectively against established S. marcescens biofilms.
Topics: Anti-Bacterial Agents; Biofilms; Biomass; Chloramphenicol; Humans; Microbial Viability; Polysorbates; Serratia marcescens; Triterpenes; Ursolic Acid
PubMed: 28356113
DOI: 10.1186/s12941-017-0192-2 -
Mikrobiologiia 2014A widespread bacterium Serratia marcescens (family Enterobacteriaceae) is an opportunistic and exhibits multiple drug resistance. Active removal of antibiotics and other... (Review)
Review
A widespread bacterium Serratia marcescens (family Enterobacteriaceae) is an opportunistic and exhibits multiple drug resistance. Active removal of antibiotics and other antimicrobials from pathogen and exhibits multiple drug resistance. Active removal of antibiotics and other antimicrobials from the cells by efflux systems is one of the mechanisms responsible for microbial resistance to these compounds. Among enterobacteria, efflux systems of Escherichia coli and Salmonella enterica var. Typhimurium have been studied most extensively. Few efflux systems that belong to different families have been reported for S. marcescens. In this review, we analyzed available literature about S. marcescens efflux systems and carried out the comparative analysis of the genes encoding the RND type systems in different Serratia species and in other enterobacteria. Bioinformatical analysis of the S. marcescens genome allowed us to identify the previously unknown efflux systems based on their homology with the relevant E. coli genes. Identification of additional efflux systems in S. marcescens genome will promote our understanding of physiology of these bacteria, will detect new molecular mechanisms of resistance and will reveal their resistance potential.
Topics: Drug Resistance, Bacterial; Genes, Bacterial; Genome, Bacterial; Serratia marcescens
PubMed: 25423729
DOI: No ID Found -
Microbiology Spectrum Jun 2022Prodigiosin possesses antibacterial activities, but as a highly hydrophobic compound, it raised the question about how Serratia marcescens introduce this compound to...
Prodigiosin possesses antibacterial activities, but as a highly hydrophobic compound, it raised the question about how Serratia marcescens introduce this compound to other microbes. Here, we demonstrate that the production of prodigiosin by newly isolated S. marcescens RH10 correlates with its antibacterial activity against a multidrug-resistant strain of S. aureus, with this pathogen's viability decreasing 6-log over 24 h. While S. marcescens RH10 does secrete membrane vesicles that carry prodigiosin, this antibiotic was not active in this form, with 5 mg/L prodigiosin leading to only a 1.22-fold reduction in the S. aureus viability while the same quantity of purified prodigiosin led to a 2800-fold reduction. Contact assays, however, showed increased activity, with a 3-log loss in the S. aureus viabilities in only 6 h as long as production of prodigiosin occurred. The role of prodigiosin was confirmed further by generating an isogenic Δ mutant in S. marcescens RH10, based on the draft genome sequence reported here, to inhibit the synthesis of prodigiosin. In all experiments performed, this mutant was unable to kill S. aureus. Finally, the possibility that the type VI secretion system present in S. marcescens may also be important was also explored as it is known to be used by this strain to kill other microbes. The results here, however, found no obvious activity against S. aureus. In conclusion, the results presented here show prodigiosin requires both cell-to-cell contact and synthesis for it to be effective as an antibiotic for its native host. The antibacterial activities of prodigiosin are well-established but, as a hydrophobic molecule, the mechanisms used to introduce it to susceptible microbes has never been studied. We found here, in contrast to violacein, another hydrophobic antibiotic that can be transferred using membrane vesicles (MVs), prodigiosin is also carried from Serratia marcescens in MVs released but its resulting activities were severely mitigated compared to the freely added compound, suggesting it is more tightly bound to the MVs than violacein. This led us to hypothesize that cell-to-cell contact is needed, which we demonstrate here. As well, we show synthesis of prodigiosin is needed for it to be effective. As violacein- and prodigiosin-producing bacterial strains are both beneficial to amphibians, where they help protect the skin against pathogens, the findings presented here provide an important ecological perspective as they show the mechanisms used differ according to the antibacterial produced.
Topics: Anti-Bacterial Agents; Prodigiosin; Serratia marcescens; Staphylococcus aureus
PubMed: 35435740
DOI: 10.1128/spectrum.00607-22 -
Journal of Biotechnology Nov 2022Heavy metal contamination is a global issue, with cadmium (Cd) and its treatment becoming major environmental challenge that could be solved by microbial restoration, an...
Heavy metal contamination is a global issue, with cadmium (Cd) and its treatment becoming major environmental challenge that could be solved by microbial restoration, an eco-friendly technique. Serratia marcescens KMR-3 exhibits high tolerance and removal rate of Cd (≤500 mg/L). Here, we aimed to explore mechanisms underlying tolerance to and removal of Cd by KMR-3. Scanning electron microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectrometry were conducted to analyze characteristics of the KMR-3 biofilm and Cd combined forms. The results revealed varying degrees of cell adhesion, membrane thickening, and shrinkage on the surface of the bacteria. The binding elements, electronic binding energy, and functional groups on the surface of the bacteria exhibited changes. Furthermore, the biofilm amount following treatment with Cd was 1.5-3 times higher than that in the controls, treatment with Cd substantially enhanced biofilm generation and increased Cd adsorption. Cd adsorption by its own secondary metabolite prodigiosin produced by KMR-3 was enhanced by 19.5 % compared with that observed without prodigiosin. Through transcriptome sequencing and RT-qPCR, we observed that Znu protein-chelating system regulated gene expression (znuA, znuB, and znuC), and the efflux mechanism of the P-type ATPase regulated the expression of genes (zntA, zntB, and zntR), which were significantly enhanced. Through the combined action of various strategies, KMR-3 demonstrated a high tolerance and removal ability of Cd, providing a theoretical basis to treat Cd pollution.
Topics: Serratia marcescens; Prodigiosin; Cadmium; Metals, Heavy; P-type ATPases
PubMed: 36184003
DOI: 10.1016/j.jbiotec.2022.09.019