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Physical Chemistry Chemical Physics :... May 2022
Topics: Humans; Lasers; Spectrum Analysis; Time Factors
PubMed: 35575020
DOI: 10.1039/d2cp90063g -
Journal of Biophotonics Jan 2013
Topics: Animals; Endoscopy; Humans; Infrared Rays; Neoplasms; Optical Tweezers; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis; Spectrum Analysis, Raman; Vibration
PubMed: 23288708
DOI: 10.1002/jbio.201300502 -
Biomedical Instrumentation & Technology 2011
Topics: Equipment Design; Equipment Failure Analysis; Spectrum Analysis
PubMed: 21466334
DOI: 10.2345/0899-8205-45.2.139 -
Critical Reviews in Analytical Chemistry May 2017Vibrational spectroscopy (Infrared (IR) and Raman) and, in particular, micro-spectroscopy and micro-spectroscopic imaging have been used to characterize developmental... (Review)
Review
Vibrational spectroscopy (Infrared (IR) and Raman) and, in particular, micro-spectroscopy and micro-spectroscopic imaging have been used to characterize developmental changes in tissues, to monitor these changes in cell cultures and to detect disease and drug-induced modifications. The conventional methods for biochemical and histophatological tissue characterization necessitate complex and "time-consuming" sample manipulations and the results are rarely quantifiable. The spectroscopy of molecular vibrations using mid-IR or Raman techniques has been applied to samples of human tissue. This article reviews the application of these vibrational spectroscopic techniques for analysis of biological tissue published between 2005 and 2015.
Topics: Humans; Neoplasms; Spectrophotometry, Infrared; Spectrum Analysis; Spectrum Analysis, Raman; Vibration
PubMed: 27786540
DOI: 10.1080/10408347.2016.1253454 -
Methods in Molecular Biology (Clifton,... 2017The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and is a critical factor in many clinical...
The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and is a critical factor in many clinical applications. The large number and variety of techniques employed for this purpose is therefore not surprising. The selection of a suitable assay is dependent on such factors as the level of sensitivity required, the presence of interfering agents, and the composition of the protein itself. Here, protocols for the most commonly used protein determination methodologies are outlined, as well as for the more recently adapted technique of quantitative immuno-Polymerase Chain Reaction.
Topics: Blotting, Western; Electrophoresis, Polyacrylamide Gel; Immunoassay; Polymerase Chain Reaction; Proteins; Spectrophotometry; Spectrum Analysis; Staining and Labeling
PubMed: 27730556
DOI: 10.1007/978-1-4939-6412-3_12 -
Applied Spectroscopy Jan 2003
Review
Topics: Algorithms; Chemistry Techniques, Analytical; Models, Molecular; Spectrum Analysis; Spectrum Analysis, Raman; Stochastic Processes
PubMed: 14610929
DOI: 10.1366/000370203321165133 -
Biochimica Et Biophysica Acta Jan 2015
Topics: Energy Metabolism; Spectrophotometry, Infrared; Spectrum Analysis; Spectrum Analysis, Raman; Vibration
PubMed: 25448165
DOI: 10.1016/j.bbabio.2014.10.003 -
Annual Review of Analytical Chemistry... 2009The scanning tunneling microscope (STM) has revolutionized our ability to explore and manipulate atomic-scale solid surfaces. In addition to its unparalleled spatial... (Review)
Review
The scanning tunneling microscope (STM) has revolutionized our ability to explore and manipulate atomic-scale solid surfaces. In addition to its unparalleled spatial power, the STM can study dynamical processes, such as molecular conformational changes, by recording current traces as a function of time. It can also be employed to measure the physical properties of molecules or nanostructures down to the atomic scale. Combining STM imaging with measurement of current-voltage (I-V) characteristics [i.e., scanning tunneling spectroscopy (STS)] at similar resolution makes it possible to obtain a detailed map of the electronic structure of a surface. For many years, STM lacked chemical specificity; however, the recent development of STM-IETS (inelastic electron tunneling spectroscopy) has allowed us to measure the vibrational spectrum of a single molecule. This review introduces and illustrates these recent developments with a few simple scholarly examples.
Topics: Microscopy, Scanning Tunneling; Spectrum Analysis
PubMed: 20636053
DOI: 10.1146/annurev-anchem-060908-155213 -
Annual Review of Physical Chemistry 1992
Review
Topics: Mathematics; Models, Theoretical; Spectrum Analysis; Spectrum Analysis, Raman; Time Factors
PubMed: 1463575
DOI: 10.1146/annurev.pc.43.100192.002433 -
Cold Spring Harbor Protocols Apr 2015Image correlation spectroscopy (ICS) was developed as the imaging analog of fluorescence correlation spectroscopy. Using standard fluorescence microscopy image series as... (Review)
Review
Image correlation spectroscopy (ICS) was developed as the imaging analog of fluorescence correlation spectroscopy. Using standard fluorescence microscopy image series as input, different versions of ICS can be used to extract parameters on the molecular transport properties (diffusion and flow) and oligomerization state for fluorescently labeled species in cells. This review introduces the various forms of spatial and temporal ICS and discusses application of these methods to reveal properties of the biomolecules that can be measured from standard fluorescence image time series sampled from cells and neurons.
Topics: Animals; Humans; Image Processing, Computer-Assisted; Spectrometry, Fluorescence; Spectrum Analysis; Time Factors; User-Computer Interface
PubMed: 25834267
DOI: 10.1101/pdb.top086124