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BMJ Case Reports Aug 2019A rare case of endophthalmitis with intraocular foreign body (IOFB) was managed successfully by vitrectomy, IOFB removal and intravitreal antibiotics with steroids....
A rare case of endophthalmitis with intraocular foreign body (IOFB) was managed successfully by vitrectomy, IOFB removal and intravitreal antibiotics with steroids. Intraoperatively, the inferior retina was noted to be pale, possibly secondary to arteriolar occlusion/inflammation. This pale retina detached while peeling the vitreous, but spontaneously reattached postoperatively within a week. The case report describes the natural course of an iatrogenic detachment of pallid retina and the outcome of an uncommon ocular infection.
Topics: Accidents, Occupational; Adult; Anti-Bacterial Agents; Endophthalmitis; Eye Foreign Bodies; Eye Infections, Bacterial; Eye Injuries, Penetrating; Humans; Male; Retinal Detachment; Treatment Outcome; Visual Acuity; Vitrectomy
PubMed: 31434672
DOI: 10.1136/bcr-2019-230126 -
Microbiology Resource Announcements Oct 2023This report describes the genome sequence of the BAU_KME002 strain isolated in Bangladesh in 2021 from a chicken egg surface. Our assembled genome had 50 contigs, an...
This report describes the genome sequence of the BAU_KME002 strain isolated in Bangladesh in 2021 from a chicken egg surface. Our assembled genome had 50 contigs, an estimated genome length of 2,866,882 bp (with coverage of 90.0×), 36 predicted antibiotic resistance genes, and 28 predicted virulence factor genes.
PubMed: 37737608
DOI: 10.1128/MRA.00555-23 -
Microbiology Resource Announcements Nov 2023We announce the genome sequence of the MTR_B001 strain isolated from the breast muscle of a chicken in 2022 in Bangladesh. This assembled genome had an estimated length...
We announce the genome sequence of the MTR_B001 strain isolated from the breast muscle of a chicken in 2022 in Bangladesh. This assembled genome had an estimated length of 2,889,393 bp (with 50× genome coverage), 15 contigs, 36 predicted antibiotic resistance genes, and 27 predicted virulence factor genes.
PubMed: 37846982
DOI: 10.1128/MRA.00647-23 -
Gene May 2023Staphylococcus gallinarum FCW1 was isolated from naturally fermented coconut-water and identified by biochemical and molecular methods. Probiotic characterization and...
Staphylococcus gallinarum FCW1 was isolated from naturally fermented coconut-water and identified by biochemical and molecular methods. Probiotic characterization and safety assessment were conducted through a series of in vitro tests. A high survival rate was observed when the strain was tested for resistance to bile, lysozyme, simulated gastric and intestinal fluid, phenol, and different temperature and salt concentrations. The strain showed antagonism against some pathogens, was susceptible to all antibiotics tested except penicillin, and showed no hemolytic and DNase activity. Hydrophobicity, autoaggregation, biofilm formation, and antioxidation tests indicated that the strain possessed a high adhesive and antioxidant ability. Enzymatic activity was used to evaluate the metabolic capacities of the strain. In-vivo experiment on zebrafish was performed to check its safety status. The whole-genome sequencing indicated that the genome contained 2,880,305 bp with a GC content of 33.23%. The genome annotation confirmed the presence of probiotic-associated genes and genes for oxalate degradation, sulfate reduction, acetate metabolism, and ammonium transport in the FCW1 strain, adding to the theory that this strain may be helpful in treating kidney stones. This study revealed that the strain FCW1 might be an excellent potential probiotic in developing fermented coconut beverages and treating and preventing kidney stone disease.
Topics: Animals; Cocos; Zebrafish; Staphylococcus; Genomics
PubMed: 36907276
DOI: 10.1016/j.gene.2023.147356 -
International Journal of Medical... Jan 2014The Staphylococcus epidermidis derived epidermin was the first lantibiotic that has been shown to be ribosomally synthesized and posttranslationally modified. Together... (Review)
Review
The Staphylococcus epidermidis derived epidermin was the first lantibiotic that has been shown to be ribosomally synthesized and posttranslationally modified. Together with gallidermin, produced by Staphylococcus gallinarum, they belong to the large class of cationic antimicrobial peptides (CAMPs) that act against a broad spectrum of Gram-positive bacteria. Here we describe the genetic organization, biosynthesis and modification, excretion, extracellular activation of the modified pre-peptide by proteolytic processing, self-protection of the producer, gene regulation, structure, and the mode of action of gallidermin and epidermin. We also address mechanisms of bacterial tolerance to these lantibiotics and other CAMPs. Particularly gallidermin has a high potential for therapeutic application, as it is active against methicillin-resistant Staphylococcus aureus strains (MRSA) and as it is able to prevent biofilm formation at sublethal concentrations.
Topics: Antimicrobial Cationic Peptides; Bacteriocins; Biosynthetic Pathways; Gene Expression Regulation, Bacterial; Gram-Positive Bacteria; Gram-Positive Bacterial Infections; Humans; Peptides; Protein Processing, Post-Translational; Staphylococcus
PubMed: 24119540
DOI: 10.1016/j.ijmm.2013.08.012 -
Pathogens (Basel, Switzerland) Apr 2024A novel coagulase-negative strain (H164) was isolated from soymilk in Taiwan. Comparative sequence analysis of the 16S rRNA gene revealed that the H164 strain is a...
A novel coagulase-negative strain (H164) was isolated from soymilk in Taiwan. Comparative sequence analysis of the 16S rRNA gene revealed that the H164 strain is a member of the genus . We used multilocus sequence analysis (MLSA) and phylogenomic analyses to demonstrate that the novel strain was closely related to , , , and . The average nucleotide identity and digital DNA-DNA hybridization values between H164 and its closest relatives were <95% and <70%, respectively. The H164 strain could also be distinguished from its closest relatives by the fermentation of -fructose, -maltose, -trehalose, and -mannitol, as well as by the activities of α-glucosidase and alkaline phosphatase. The major cellular fatty acids were C15:0 iso and C15:0 anteiso, and the predominant menaquinones were MK-7 and MK-8, respectively. The major cellular fatty acids and predominant menaquinones were C iso and C anteiso and MK-7 and MK-8, respectively. In conclusion, this strain represents a novel species, named sp. nov., with the type strain H164 (=BCRC 81404 = NBRC 116174).
PubMed: 38668298
DOI: 10.3390/pathogens13040343 -
Veterinary Microbiology Feb 1986Of 136 strains of coagulase-negative staphylococci isolated from healthy and sick human beings, goats, sheep, antelope and other animals, 88 (64.7%) were Staphylococcus... (Comparative Study)
Comparative Study
Of 136 strains of coagulase-negative staphylococci isolated from healthy and sick human beings, goats, sheep, antelope and other animals, 88 (64.7%) were Staphylococcus sciuri and 35 (25.7%) were S. lentus and the remainder Staphylococcus gallinarum. The strains of S. sciuri were isolated from humans with boils and wounds, goats with pestes des petits ruminants (PPR) and dogs with nasal discharge. One isolate of S. gallinarum came from a fowl with chronic respiratory disease and 11 others were isolated from goats. The characteristics of S. sciuri, S. lentus and S. gallinarum isolated from different sources were similar.
Topics: Animal Diseases; Animals; Deer; Dogs; Goats; Humans; Poultry; Sheep; Species Specificity; Staphylococcal Infections; Staphylococcus
PubMed: 3705446
DOI: 10.1016/0378-1135(86)90019-2 -
Brazilian Journal of Microbiology :... Dec 2022This research aimed to identify the diversity of bacterial species of the genus Staphylococcus spp. in subclinical mastitis in dairy herds in the state of Piauí,...
This research aimed to identify the diversity of bacterial species of the genus Staphylococcus spp. in subclinical mastitis in dairy herds in the state of Piauí, Northeastern Brazil, and to evaluate the phenotypic and genotypic resistance profile. Samples were obtained from a total of 17 dairy farms, amounting to 321 positive samples in the California Mastitis Test. Staphylococcus spp. were identified by matrix-assisted laser desorption ionization time-of-flight mass spectroscopy. Subsequently, an antibiogram was performed, and a polymerase chain reaction was carried out to screen for resistance genes in the isolates. Among all the isolates, 59.45% (110/185) belonged to the Staphylococcus genus. Moreover, the following Staphylococcus spp. were identified Staphylococcus aureus, 68.1% (75/110); Staphylococcus chromogenes, 12.7% (14/110); Staphylococcus epidermidis, 5.4% (6/110); Staphylococcus sciuri, 4.5% (5/110); Staphylococcus warneri, 2.7% (3/110); Staphylococcus haemolyticus, 1.8% (2/110); Staphylococcus hominis, 1.8% (2/110); Staphylococcus arlettae, 0.9% (1/110); Staphylococcus capitis, 0.9% (1/110); and Staphylococcus gallinarum, 0.9% (1/110). The antibiogram showed a high frequency of resistance to penicillin and ampicillin, 70.0% (77/110) and 61.8% (68/110), respectively, and a low frequency of resistance to gentamicin and vancomycin, 10.9% (12/110) and 11.8% (13/110), respectively. In the genotypic tests for the different species of Staphylococcus spp., the occurrence of the blaZ gene was observed in 60.9% (67/110) of the isolates, followed by tetL and tetM, both with 20.0% (22/110) each, and the mecA and vanB genes were detected in 0.9% (1/110) of the samples. The identification of all Staphylococcus species isolated from subclinical mastitis cases and the phenotypic and genotypic resistance characterization in these isolates is of great importance for dairy farming in the state of Piauí, as well as for public health.
Topics: Cattle; Animals; Female; Humans; Mastitis, Bovine; Methicillin-Resistant Staphylococcus aureus; Brazil; Staphylococcus; Staphylococcal Infections; Microbial Sensitivity Tests; Milk; Anti-Bacterial Agents
PubMed: 36074251
DOI: 10.1007/s42770-022-00822-1 -
International Journal of Systematic and... May 2000During the collection of airborne bacteria in a museum in England some bacterial strains were isolated which due to their fatty acid profiles were clearly identified as...
During the collection of airborne bacteria in a museum in England some bacterial strains were isolated which due to their fatty acid profiles were clearly identified as members of the genus Staphylococcus. As fatty acid compositions of coagulase-negative staphylococci are very similar, differing only in quantities but not in qualities, further identification at the species level without a fatty acid database was not achieved. Investigation of the isolates using the Staph ID 32 API system resulted in an identification of the isolates as Staphylococcus epidermidis (probabilities of 79.7-95.5%). For further genotypic characterization of these isolates, some Staphylococcus epidermidis strains from different sources and the type strains of Staphylococcus aureus, Staphylococcus capitis, Staphylococcus epidermidis, Staphylococcus gallinarum, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus warneri and Staphylococcus xylosus were subjected to repetitive-sequence PCR, including enterobacterial repetitive intergenic consensus (ERIC) PCR, BOX-PCR and repetitive extragenic palindromic unit sequence (REP) PCR. ERIC- and BOX-PCR yielded a species-specific banding pattern for all Staphylococcus epidermidis strains. Furthermore, all staphylococcal reference strains investigated exhibited distinct banding patterns, clearly distinguishable from that of Staphylococcus epidermidis. No species-specific banding patterns could be observed after REP-PCR. As species identification of coagulase-negative staphylococci by fatty acid analyses and biochemical tests is known to be difficult ERIC- and BOX-PCR seem to be excellent tools for the identification of Staphylococcus epidermidis isolates.
Topics: Air Microbiology; Fatty Acids; Phenotype; Polymerase Chain Reaction; Species Specificity; Staphylococcus epidermidis; Time Factors
PubMed: 10843049
DOI: 10.1099/00207713-50-3-1087 -
Genome Announcements Jun 2015Staphylococcus gallinarum DSM 20610(T) is a rare pathogen in humans. The increasing relevance of human health prompted us to determine the genomic sequence of...
Staphylococcus gallinarum DSM 20610(T) is a rare pathogen in humans. The increasing relevance of human health prompted us to determine the genomic sequence of S. gallinarum. The complete genome sequence of S. gallinarum includes a genome of 3,171,720 bp (33.02% G+C content) without any plasmids.
PubMed: 26044428
DOI: 10.1128/genomeA.00580-15