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Eukaryotic Cell Sep 2007Bloodstream form Trypanosoma theileri degrades glucose to acetate (47%) and succinate (45%) and, therefore, does not solely rely on glycolysis for ATP production. This...
Bloodstream form Trypanosoma theileri degrades glucose to acetate (47%) and succinate (45%) and, therefore, does not solely rely on glycolysis for ATP production. This trypanosomatid does not use amino acids for energy metabolism. These results refute the prevailing hypothesis that substrate availability determines the type of energy metabolism of trypanosomatids.
Topics: Acetates; Animals; Blood; Cattle; Energy Metabolism; Glucose; Magnetic Resonance Spectroscopy; Succinic Acid; Trypanosoma
PubMed: 17644652
DOI: 10.1128/EC.00130-07 -
Parasitology International Apr 2020Callithrix jacchus and C. penicillata marmosets are invasive to the state of Rio de Janeiro, Brazil, threatening the native and vulnerable C. aurita. Both invasive...
Molecular and morphometric identification of Trypanosoma (Megatrypanum) minasense in blood samples of marmosets (Callithrix: Callithrichidae) from the city of Rio de Janeiro, Brazil.
Callithrix jacchus and C. penicillata marmosets are invasive to the state of Rio de Janeiro, Brazil, threatening the native and vulnerable C. aurita. Both invasive species can be hosts of Trypanosoma cruzi, T. minasense, T. rangeli and T. devei. We aim to investigate the occurrence of trypanosomatids in Callithrix sp. from Jardim Botânico do Rio de Janeiro, located in a central and populous area of the city. Fifteen marmosets were captured. Blood samples were collected for light microscopy and molecular genetics analysis. Parasites morphometric values were evaluated for species identification. DNA was extracted from blood samples by phenol-chloroform method, for partial amplification of the 18S rRNA gene. PCR products were sequenced and aligned using BLAST®. A maximum likelihood phylogenetic tree was constructed to analyze the proximity between the observed sequences. By light microscopy, trypomastigotes were detected in five of the fifteen marmosets. Morphometric measurements and size polymorphism corresponded to those previously described for T. minasense. The DNA sequences of approximately 600 base pairs of the 18S rRNA gene were obtained for three samples with 99% identity with T. minasense sequence, forming a cluster in the phylogenetic tree and corroborating morphometric analysis. Trypanosoma minasense is a highly specific parasite to non-human primates considered as non-pathogenic. There is no evidence of infection in humans and these parasite findings from invasive marmosets do not support additional risks for the native species.
Topics: Animals; Brazil; Callithrix; Monkey Diseases; Trypanosoma; Trypanosomiasis
PubMed: 31669293
DOI: 10.1016/j.parint.2019.101999 -
Biological Chemistry May 2020The evolution of mitochondrial protein import and the systems that mediate it marks the boundary between the endosymbiotic ancestor of mitochondria and a true organelle... (Review)
Review
The evolution of mitochondrial protein import and the systems that mediate it marks the boundary between the endosymbiotic ancestor of mitochondria and a true organelle that is under the control of the nucleus. Protein import has been studied in great detail in Saccharomyces cerevisiae. More recently, it has also been extensively investigated in the parasitic protozoan Trypanosoma brucei, making it arguably the second best studied system. A comparative analysis of the protein import complexes of yeast and trypanosomes is provided. Together with data from other systems, this allows to reconstruct the ancestral features of import complexes that were present in the last eukaryotic common ancestor (LECA) and to identify which subunits were added later in evolution. How these data can be translated into plausible scenarios is discussed, providing insights into the evolution of (i) outer membrane protein import receptors, (ii) proteins involved in biogenesis of α-helically anchored outer membrane proteins, and (iii) of the intermembrane space import and assembly system. Finally, it is shown that the unusual presequence-associated import motor of trypanosomes suggests a scenario of how the two ancestral inner membrane protein translocases present in LECA evolved into the single bifunctional one found in extant trypanosomes.
Topics: Mitochondrial Proteins; Protozoan Proteins; Trypanosoma
PubMed: 32142472
DOI: 10.1515/hsz-2019-0444 -
The Journal of Protozoology Nov 1969
Topics: Animals; Microscopy, Electron; Organoids; Trypanosoma
PubMed: 5362390
DOI: 10.1111/j.1550-7408.1969.tb02335.x -
Veterinary Parasitology Jun 1994The phylogenetic relatedness of 15 stocks of Trypanosoma evansi, three stocks of Trypanosoma equiperdum and one stock of Trypanosoma brucei brucei was determined using...
The phylogenetic relatedness of 15 stocks of Trypanosoma evansi, three stocks of Trypanosoma equiperdum and one stock of Trypanosoma brucei brucei was determined using Southern blot analysis of restriction enzyme digested DNA, probed with two repetitive DNA sequences from T. b. brucei. A dendrogram derived by cluster analysis of restriction fragment length polymorphism (RFLP) revealed three groups of related stocks. Group 1 included 14 stocks of T. evansi and one stock of T. equiperdum. Group 2 included two stocks of T. equiperdum and one stock of T. evansi. Group 3 included the one stock of T. brucei brucei. Group 2 is more closely related to Group 3 than Group 1, by analysis of the banding patterns. Further analysis of the T. evansi in Group 1 revealed that the patterns of isolates from different provinces in China were identical, but differed from T. evansi isolated from Africa, South America and the Philippines. These results provide insight into the origins of T. evansi and suggest that RFLP may be a useful means of distinguishing closely related trypanosomes.
Topics: Animals; Cluster Analysis; DNA Probes; DNA, Protozoan; Phylogeny; Polymorphism, Restriction Fragment Length; Repetitive Sequences, Nucleic Acid; Trypanosoma; Trypanosoma brucei brucei; Trypanosomiasis
PubMed: 7975115
DOI: 10.1016/0304-4017(94)90183-x -
Experimental Parasitology Aug 1969
Topics: Animals; Mice; Morphogenesis; Radiation Effects; Trypanosoma
PubMed: 5362579
DOI: 10.1016/0014-4894(69)90062-9 -
Annals of Tropical Medicine and... Jun 1973
Topics: Animals; Birds; Blood; Nigeria; Trypanosoma
PubMed: 4722424
DOI: 10.1080/00034983.1973.11686880 -
Transactions of the Royal Society of... 1972
Topics: Animals; Culture Media; Haplorhini; Insect Vectors; Macaca; Monkey Diseases; Pigments, Biological; Triatominae; Trypanosoma; Trypanosomiasis
PubMed: 4627177
DOI: 10.1016/0035-9203(72)90309-4 -
Memorias Do Instituto Oswaldo Cruz 1991A trypanosome strain isolated from a sylvatic rodent (Echimys dasythrix) from Santa Catarina Island (Santa Catarina State, Brazil) was characterized by the following...
Trypanosoma rangeli (Tejera, 1920) isolated from a sylvatic rodent (Echimys dasythrix) in Santa Catarina Island, Santa Catarina State: first report of this trypanosome in southern Brazil.
A trypanosome strain isolated from a sylvatic rodent (Echimys dasythrix) from Santa Catarina Island (Santa Catarina State, Brazil) was characterized by the following methods: experimental transmission and development in invertebrate and vertebrate hosts, morphometry, cross protection, complement sensitivity, lectin agglutination and isoenzyme profiles. Comparisons were made with standard Trypanosoma cruzi and T. rangeli strains. All methods except isoenzyme analysis led to the identification of the isolate as T. rangeli. The isoenzyme differences found could be explained on the basis of polymorphism. Therefore this is the first report of T. rangeli in southern Brazil, increasing the geographical distribution of this parasite.
Topics: Animals; Brazil; Disease Vectors; Host-Parasite Interactions; Isoenzymes; Mice; Rodentia; Trypanosoma; Trypanosomiasis
PubMed: 1842404
DOI: 10.1590/s0074-02761991000100012 -
Acta Tropica Apr 1993Trypanosoma rangeli, the only trypanosome besides Trypanosoma cruzi to infect humans in the Americas, shows an important geographical overlap with the agent of Chagas... (Comparative Study)
Comparative Study
Trypanosoma rangeli, the only trypanosome besides Trypanosoma cruzi to infect humans in the Americas, shows an important geographical overlap with the agent of Chagas disease, and its taxonomic position has been the source of some controversy. This study utilizes beta-tubulin gene sequences for investigating the phylogeny of this species. All trees, produced with the different algorithms utilized, always grouped T. rangeli with Trypanosoma brucei in preference to T. cruzi. In addition evidence suggesting that the genus Trypanosoma may be polyphyletic was found.
Topics: Animals; Base Sequence; Humans; Molecular Sequence Data; Trypanosoma; Trypanosoma brucei brucei; Trypanosoma cruzi; Tubulin
PubMed: 8098902
DOI: 10.1016/0001-706x(93)90022-4