-
Ugeskrift For Laeger May 1995
Topics: Hot Temperature; Humans; Vibrio Infections; Vibrionaceae; Water Microbiology
PubMed: 7770978
DOI: No ID Found -
Annual Review of Microbiology 1988
Review
Topics: Aeromonas; Animals; Bacterial Infections; Humans; Vibrionaceae; Virulence
PubMed: 3059998
DOI: 10.1146/annurev.mi.42.100188.002143 -
PLoS Genetics Mar 2018Vibrio cholerae, the causative agent of the cholera disease, is commonly used as a model organism for the study of bacteria with multipartite genomes. Its two...
Vibrio cholerae, the causative agent of the cholera disease, is commonly used as a model organism for the study of bacteria with multipartite genomes. Its two chromosomes of different sizes initiate their DNA replication at distinct time points in the cell cycle and terminate in synchrony. In this study, the time-delayed start of Chr2 was verified in a synchronized cell population. This replication pattern suggests two possible regulation mechanisms for other Vibrio species with different sized secondary chromosomes: Either all Chr2 start DNA replication with a fixed delay after Chr1 initiation, or the timepoint at which Chr2 initiates varies such that termination of chromosomal replication occurs in synchrony. We investigated these two models and revealed that the two chromosomes of various Vibrionaceae species terminate in synchrony while Chr2-initiation timing relative to Chr1 is variable. Moreover, the sequence and function of the Chr2-triggering crtS site recently discovered in V. cholerae were found to be conserved, explaining the observed timing mechanism. Our results suggest that it is beneficial for bacterial cells with multiple chromosomes to synchronize their replication termination, potentially to optimize chromosome related processes as dimer resolution or segregation.
Topics: Bacterial Proteins; Biological Evolution; Chromosomes, Bacterial; DNA Replication; Vibrio cholerae; Vibrionaceae
PubMed: 29505558
DOI: 10.1371/journal.pgen.1007251 -
International Journal of Systematic and... Nov 2003The family Vibrionaceae is considered to be one of the most diverse and well-studied groups of bacteria. Here, evolution is assessed within the Vibrionaceae to determine...
The family Vibrionaceae is considered to be one of the most diverse and well-studied groups of bacteria. Here, evolution is assessed within the Vibrionaceae to determine whether multiple origins of eukaryotic associations have occurred within this diverse group of bacteria. Analyses were based on a large molecular dataset, along with a matrix that consisted of 100 biochemical and restriction digest characters. By using direct optimization methods to analyse both datasets individually and in combination, a total-evidence cladogram has been produced, which supports the hypothesis that several important symbionts (both mutualistic and pathogenic) within the Vibrionaceae are not monophyletic. This leads us to consider that symbiosis (and subsequently, associations with Eukarya) has evolved multiple times within the Vibrionaceae lineage.
Topics: Biological Evolution; DNA, Ribosomal; Genetic Variation; Phylogeny; Polymorphism, Restriction Fragment Length; RNA, Ribosomal, 5S; Restriction Mapping; Symbiosis; Vibrionaceae
PubMed: 14657139
DOI: 10.1099/ijs.0.02792-0 -
Systematic and Applied Microbiology Jul 2017Two phylogenetically distinct Vibrionaceae strains C4II189 and C4V358 isolated from reef seawater off Ishigaki Island, Japan, in 2014 were studied with advanced...
Thaumasiovibrio occultus gen. nov. sp. nov. and Thaumasiovibrio subtropicus sp. nov. within the family Vibrionaceae, isolated from coral reef seawater off Ishigaki Island, Japan.
Two phylogenetically distinct Vibrionaceae strains C4II189 and C4V358 isolated from reef seawater off Ishigaki Island, Japan, in 2014 were studied with advanced genome-based taxonomy approaches. All aspects of phylogenetic (16S rRNA phylogeny, MLSA), phenotypic and genetic (ANI, DDH, AAI, and the number of core genes) cohesions between the two identified species were high enough to propose them as members of a new genus within the family Vibrionaceae. Consequently, an eighth genus Thaumasiovibrio gen. nov. is proposed that contains two new species Thaumasiovibrio occultus sp. nov. strain C4II189 (=DSM 101554=JCM 31629) (type species) and Thaumasiovibrio subtropicus sp. nov. strain C4V358 (=DSM 101555=JCM 31630). Thaumasiovibrio species were phylogenetically distinct from the other Vibrionaceae species based on pyrH gene sequences. The combination of catalase negative, sensitivity to vibriostatic agent O/129, and green colony formation on TCBS for the phylogenetically affiliated strains was the diagnostic features for the current tentative identification of this genus.
Topics: Animals; Anthozoa; Base Composition; Coral Reefs; DNA, Bacterial; Japan; Multilocus Sequence Typing; Phylogeny; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Vibrionaceae
PubMed: 28648725
DOI: 10.1016/j.syapm.2017.04.003 -
Canadian Journal of Microbiology Feb 1991It has previously been reported that norspermidine, one of the unusual polyamines, is present in Vibrio species. To expand this observation, the cellular polyamine...
It has previously been reported that norspermidine, one of the unusual polyamines, is present in Vibrio species. To expand this observation, the cellular polyamine compositions of additional species and strains in the family Vibrionaceae (Vibrio, Photobacterium, Listonella, and Shewanella) as well as Aeromonas species and Plesiomonas shigelloides, which have been proposed to be excluded from Vibrionacea, were determined by using gas-liquid chromatography. Some Vibrio species previously reported were reexamined under the same conditions, and their results are included in this report. Norspermidine was detected as a major triamine in 23 of 24 Vibrio species, all of 4 Listonella species, and 3 of 5 Photobacterium species. Vibrio costicola, Photobacterium fischeri, and Photobacterium phosphoreum contained no norspermidine. Listonella species were indistinguishable from Vibrio species in their polyamine profiles. However, Schewanella putrefaciens ATCC 8071, formerly allocated in the genus Alteromonas, contained no norspermidine, and its polyamine profile was similar to those of four Aeromonas species, in which putrescine was exclusively found. Plesiomonas shigelloides was very similar to Escherichia coli in that putrescine and spermidine were predominant polyamines. Our data indicate that the occurrence of norspermidine may be very helpful as a generic marker in identification and classification of Vibrio and Listonella species. A gas-liquid chromatographic method with a nitrogen-selective detector was presented for rapid and sensitive detection of cellular norspermidine.
Topics: Chromatography, Gas; Polyamines; Putrescine; Species Specificity; Spermidine; Vibrionaceae
PubMed: 2059921
DOI: 10.1139/m91-022 -
Applied and Environmental Microbiology Apr 2015Microbial taxonomy is essential in all areas of microbial science. The 16S rRNA gene sequence is one of the main phylogenetic species markers; however, it does not...
Microbial taxonomy is essential in all areas of microbial science. The 16S rRNA gene sequence is one of the main phylogenetic species markers; however, it does not provide discrimination in the family Vibrionaceae, where other molecular techniques allow better interspecies resolution. Although multilocus sequence analysis (MLSA) has been used successfully in the identification of Vibrio species, the technique has several limitations. They include the fact that several locus amplifications and sequencing have to be performed, which still sometimes lead to doubtful identifications. Using an in silico approach based on genomes from 103 Vibrionaceae strains, we demonstrate here the high resolution of the fur gene in the identification of Vibrionaceae species and its usefulness as a phylogenetic marker. The fur gene showed within-species similarity higher than 95%, and the relationships inferred from its use were in agreement with those observed for 16S rRNA analysis and MLSA. Furthermore, we developed a fur PCR sequencing-based method that allowed identification of Vibrio species. The discovery of the phylogenetic power of the fur gene and the development of a PCR method that can be used in amplification and sequencing of the gene are of general interest whether for use alone or together with the previously suggested loci in an MLSA.
Topics: Bacterial Proteins; Base Sequence; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction; Repressor Proteins; Sequence Alignment; Vibrionaceae
PubMed: 25662978
DOI: 10.1128/AEM.00058-15 -
Reviews on Environmental Health 1997In recent years, members belonging to the genus Vibrio of the family Vibrionaceae have acquired increasing importance because of the association of several of its... (Review)
Review
In recent years, members belonging to the genus Vibrio of the family Vibrionaceae have acquired increasing importance because of the association of several of its members with human disease. The most feared of the Vibrio species is Vibrio cholerae, the causative agent of cholera, a devastating disease of global significance. Other important vibrios of medical importance are V. parahemolyticus, V. vulnificus, V. mimicus, and to a lesser extent V. fluvialis, V. furnissii, V. hollisae, and V. damsela. Recent studies have also implicated V. alginolyticus and V. metschnikovii in human disease, although their complete significance has not yet been established. The virulence of all medically important vibrios is aided by a variety of traits that help breach human defenses. In this review, we provide an overview of the environmental distribution of the pathogenic vibrios and the important virulence traits that enable them to cause disease.
Topics: Ecology; Environmental Health; Humans; Serology; Vibrio; Vibrio Infections; Virulence; Water Microbiology
PubMed: 9273923
DOI: 10.1515/reveh.1997.12.2.63 -
FEMS Microbiology Ecology Apr 2017The blue mussel (Mytilus edulis) is known as a robust bivalve species, although its larviculture appears to be highly susceptible to diseases. In this study, we isolated...
The blue mussel (Mytilus edulis) is known as a robust bivalve species, although its larviculture appears to be highly susceptible to diseases. In this study, we isolated 17 strains from induced mortality events in healthy wild-caught blue mussel adults and demonstrated that they caused between 17% and 98% mortality in blue mussel larvae in a newly developed, highly controlled immersion challenge test model. Eight of the isolates belong to the Splendidus clade of vibrios, while the other isolates belong to the genus Photobacterium. The genomes of the most virulent Vibrio isolate and the most virulent Photobacterium isolate were sequenced and contained several genes encoding factors that have previously been linked to virulence towards bivalves. In vitro tests confirmed that all 17 isolates were positive for these virulence factors. The sequenced genomes also contained a remarkably high number of multidrug resistance genes. We therefore assessed the sensitivity of all isolates to a broad range of antibiotics and found that there were indeed many strong positive correlations between the sensitivities of the isolates to different antibiotics. Our data provide an ecological insight into mass mortality in blue mussels as they indicate that wild mussels contain a reservoir of pathogenic bacteria.
Topics: Animals; Aquaculture; Mytilus edulis; Vibrio; Vibrionaceae
PubMed: 28334251
DOI: 10.1093/femsec/fix039 -
Biochimica Et Biophysica Acta Apr 2005Phosphoglucose isomerase (PGI) with a novel lysyl aminopeptidase (LysAP) activity was recently purified and characterized from Vibrio vulnificus. We showed that it...
Phosphoglucose isomerase (PGI) with a novel lysyl aminopeptidase (LysAP) activity was recently purified and characterized from Vibrio vulnificus. We showed that it cleaves the amino-terminal lysyl residue from des-Arg(10)-kallidin to produce des-Arg(9)-bradykinin, suggesting that it plays a role in virulence. A survey was conducted to determine the presence of this potential virulence-enhancing enzyme among twenty-three halotolerant human and fish pathogens from eleven species within the Vibrionaceae family, including V. vulnificus, V. parahaemolyticus, V. cholerae, Aeromonas hydrophila, and Plesiomonas shigelloides. In addition, fourteen species of non-Vibrionaceae pathogens were screened for LysAP activity. Cell lysates were partially purified by anion exchange chromatography and fractions were screened for LysAP and isomerase activities. PGI-LysAP activity was detected in chromatographic fractions from all the Vibrio species tested, but was not detected in any of the non-Vibrionaceae pathogens. Levels of isomerase and LysAP activity correlated (R(2)=0.92) for nine strains of V. vulnificus. Since the Vibrionaceae represent an important family of human and fish pathogens, our identification of PGI-LysAP activity in a broad array of vibrios may lead to the development of improved analytical methods for their identification as well as interventions to reduce the high morbidity and mortality associated with some Vibrionaceae infections in clinical, veterinary, and aquaculture settings.
Topics: Aminopeptidases; Animals; Bacteria; Bacterial Proteins; Glucose-6-Phosphate Isomerase; Humans; Vibrionaceae
PubMed: 15752701
DOI: 10.1016/j.bbapap.2004.12.013