-
The Veterinary Clinics of North... Sep 2015Exotic pet veterinarians frequently have to operate on small animals, and magnification is commonly used. Existing endoscopy equipment can be used with a mechanical arm... (Review)
Review
Exotic pet veterinarians frequently have to operate on small animals, and magnification is commonly used. Existing endoscopy equipment can be used with a mechanical arm and telescope to enable video telescope operating microscopy. The additional equipment items and their specifics are described, and several case examples are provided.
Topics: Animals; Animals, Exotic; Animals, Newborn; Microscopy, Video; Microsurgery; Telescopes
PubMed: 26117519
DOI: 10.1016/j.cvex.2015.05.007 -
Methods in Cell Biology 1998Obviously there are many variations and embellishments on the topic of quantitating your image. I have tried here to offer you a very practical guide which highlights... (Review)
Review
Obviously there are many variations and embellishments on the topic of quantitating your image. I have tried here to offer you a very practical guide which highlights some of the critical issues paying particular attention to problems which can prevent or compromise your success. These include: errors due to aspect ratio, use of automatic camera settings, improper setting of dynamic range, and use of integer arithmetic. Further information can be found in several other chapters in this volume as well as in the references below. Additionally you shouldn't overlook the technical manuals which come with your video systems. The field of video microscopy has evolved as a cooperative effort between academia and industry. As a result you will find that many of the technical support personnel from microscope, video, and image processing companies are well versed on the issue of video imaging in biology and are more than willing to assist you.
Topics: Image Enhancement; Image Processing, Computer-Assisted; Microscopy, Video
PubMed: 9500135
DOI: No ID Found -
Histochemistry and Cell Biology Aug 2014Green fluorescent protein (GFP)-based video microscopy can provide profound insight into biological processes by generating information on the 'history,' or dynamics, of... (Review)
Review
Green fluorescent protein (GFP)-based video microscopy can provide profound insight into biological processes by generating information on the 'history,' or dynamics, of the cellular structures involved in such processes in live cells. A crucial limitation of this approach, however, is that many such structures may not be resolved by light microscopy. Like more recent super-resolution techniques, correlative video-light-electron microscopy (CLEM) was developed to overcome this limitation. CLEM integrates GFP-based video microscopy and electron microscopy through a series of ancillary techniques, such as proper fixation, hybrid labeling and retracing, and so provides sufficient resolution as well as, crucially, cellular 'context' to the fluorescent dynamic structures of interest. CLEM 'multiplies' the power of video microscopy and is having an important impact in several areas cell and developmental biology. Here, we discuss potential, limitations and perspectives of correlative approaches aimed at integrating the unique insight generated by video microscopy with information from other forms of imaging.
Topics: Green Fluorescent Proteins; Microscopy, Electron; Microscopy, Fluorescence; Microscopy, Video; Staining and Labeling; Tissue Fixation
PubMed: 25030356
DOI: 10.1007/s00418-014-1249-3 -
Scientific Reports Jul 2022Total holographic characterization (THC) is presented here as an efficient, automated, label-free method of accurately identifying cell viability. THC is a...
Total holographic characterization (THC) is presented here as an efficient, automated, label-free method of accurately identifying cell viability. THC is a single-particle characterization technology that determines the size and index of refraction of individual particles using the Lorenz-Mie theory of light scattering. Although assessment of cell viability is a challenge in many applications, including biologics manufacturing, traditional approaches often include unreliable labeling with dyes and/or time consuming methods of manually counting cells. In this work we measured the viability of Saccharomyces cerevisiae yeast in the presence of various concentrations of isopropanol as a function of time. All THC measurements were performed in the native environment of the sample with no dilution or addition of labels. Holographic measurements were made with an in-line holographic microscope using a 40[Formula: see text] objective lens with plane wave illumination. We compared our results with THC to manual counting of living and dead cells as distinguished with trypan blue dye. Our findings demonstrate that THC can effectively distinguish living and dead yeast cells by the index of refraction of individual cells.
Topics: Coloring Agents; Holography; Microscopy; Microscopy, Video; Saccharomyces cerevisiae
PubMed: 35882977
DOI: 10.1038/s41598-022-17098-y -
American Journal of Clinical Pathology May 1996
Topics: Clinical Laboratory Techniques; Cytodiagnosis; Diagnostic Errors; Female; Humans; Microscopy, Video; Reproducibility of Results; Vaginal Smears
PubMed: 8623757
DOI: 10.1093/ajcp/105.5.529 -
Critical Care (London, England) Sep 2023
Topics: Humans; Microcirculation; Microscopy, Video; Critical Care
PubMed: 37700327
DOI: 10.1186/s13054-023-04642-z -
Tanpakushitsu Kakusan Koso. Protein,... May 1997
Review
Topics: Animals; Cell Biology; Cell Membrane; Cell Nucleus; Cytoplasm; Cytoplasmic Granules; Cytoskeleton; Microscopy, Video; Phagosomes
PubMed: 9170920
DOI: No ID Found -
Methods in Cell Biology 2008Three-dimensional structure of cells and organelles examined with the power of resolution of electron microscopy (EM) including EM tomography represents the average view... (Review)
Review
Three-dimensional structure of cells and organelles examined with the power of resolution of electron microscopy (EM) including EM tomography represents the average view of cell processes. More precise and detailed analysis is limited by the significant variations in the structure of cells in temporal dynamics of cellular events. Therefore EM cannot identify rare and fast events that could be extremely important for understanding molecular mechanisms underlying these cellular processes. Observation of living cells under EM is still impossible. In contrast, observations of cellular dynamics with the help of laser scanning confocal microscopes or light digitalized microscopes became an indispensable tool of cell biology. However, the resolution of even confocal microscope is limited to the half of the light wave. Therefore, in studies of dynamic cellular processes, it would be ideal to be able to combine the capability of in vivo fluorescence video microscopy with the EM. This chapter describes this technique with details and useful tricks, including the way to localize the same cell after its transfection with a protein fused with a fluorescent tag, examination under the microscope in living condition, fixation, immunolabeling, embedding, serial sectioning from the first section, and observation under EM. We also illustrate here the kinds of questions that the CVLEM approach was designed to address, as well as the particular know-how that is important for the successful application of this technique.
Topics: Animals; Cell Physiological Phenomena; Gold; Horseradish Peroxidase; Humans; Imaging, Three-Dimensional; Immunohistochemistry; Metal Nanoparticles; Microscopy, Electron; Microscopy, Fluorescence; Microscopy, Video; Microtomy; Models, Biological; Tissue Fixation
PubMed: 18617029
DOI: 10.1016/S0091-679X(08)00405-6 -
Methods in Molecular Biology (Clifton,... 2001
Review
Topics: Animals; Cytoskeleton; Microscopy, Phase-Contrast; Microscopy, Video
PubMed: 11190515
DOI: 10.1385/1-59259-051-9:031 -
Methods in Cell Biology 2003Obviously there are many variations and embellishments on the topic of quantitating your image. I have tried here to offer you a very practical guide that highlights... (Review)
Review
Obviously there are many variations and embellishments on the topic of quantitating your image. I have tried here to offer you a very practical guide that highlights some of the critical issues, paying particular attention to problems that can prevent or compromise your success. These include errors caused by aspect ratio, use of automatic camera settings, improper setting of dynamic range, and use of integer arithmetic. Further information can be found in several other chapters in this volume as well as in the references provided. In addition, you should not overlook the technical manuals that come with your imaging systems. The field of digital microscopy has evolved as a cooperative effort between academia and industry. As a result you will find that many of the technical support personnel from microscope, camera, and image-processing companies are well versed on the issue of digital imaging in biology and are more than willing to assist you.
Topics: Artifacts; Image Processing, Computer-Assisted; Microscopy, Video; Optics and Photonics; Photomicrography
PubMed: 14719338
DOI: 10.1016/s0091-679x(03)72015-9