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Zhongguo Shi Yan Xue Ye Xue Za Zhi Jun 2024This study was aimed to provide ideas for identifying the antibodies to high-frequency antigens by analyzing a female case of high-frequency antigen antibody (anti-Ku)...
OBJECTIVE
This study was aimed to provide ideas for identifying the antibodies to high-frequency antigens by analyzing a female case of high-frequency antigen antibody (anti-Ku) using serological and sequencing method.
METHODS
The methods for identification of blood group, erythrocyte antigen, screening and identification of antibody were used to detect the blood type and antibody in the proband. The proband's serum and reagent screening cells treated with Sulfhydryl reagent were applied to judge the type and characteristics of this antibodies when reacted with the regaent screening cells or proband's serum respectively. Gene sequencing was used to determine the genotype of the proband's blood group.
RESULTS
The proband's red blood cells were determined as O type RhD positive, whose serum showed strong positive reaction to antibody-screening cells and antibody identification cells with the same intensity in saline and IAT medium, however, the self-cells showed negative effect. The Direct Antihuman Globulin of proband's red blood cells also showed weak positive reaction, and the other blood types were CcEe, Jk(a+b-), P1-, Le(a-b -), Lu (a-b +), K-, k-, Kp(a-b-). Serum of the proband treated with 2-ME still react with three groups of screening cells in IAT medium. The reaction intensity of proband's serum was also unchanged with the cells modified with papain and bromelain, but showed negative effect when the cells were treated with sulfhydryl agents including DTT and 2-ME. Gene sequencing revealed that the genotype of the patient was . This patient had a rare K0 phenotype.
CONCLUSION
The rare Kell-null blood group (also known as K0) were identified by serological and molecular tests in the proband who produced both IgG and IgM type of antibody to high-frequency antigen (anti-Ku). These two methods are of great significance in the identification of this rare blood group as well as the antibody to high frequency antigen.
Topics: Humans; Female; Erythrocytes; Blood Group Antigens; Blood Grouping and Crossmatching; Genotype; Ku Autoantigen; Antibodies
PubMed: 38926983
DOI: 10.19746/j.cnki.issn.1009-2137.2024.03.035 -
Sante Publique (Vandoeuvre-les-Nancy,... 2024During the COVID-19 health crisis, the official mechanisms of democracy in health, partnership, and participation were undermined. However, mechanisms that were less...
INTRODUCTION
During the COVID-19 health crisis, the official mechanisms of democracy in health, partnership, and participation were undermined. However, mechanisms that were less visible at the institutional level developed, driven by actors in the field.
PURPOSE OF THE RESEARCH
This article aims to better understand the impacts of the epidemic on partnerships in health and in situations of disability during the period 2020–2021 Results: The participants’ responses (study 1) and narrative (study 2) were analyzed using the lexical analysis software Iramuteq. The results show that while the pandemic revealed difficulties in terms of cooperation between the health and medico-social fields, it also brought to light new operations between the actors and initiatives fostering creativity.
CONCLUSIONS
These results support the idea of a paradigm shift involving the recognition of the plurality of knowledge at work in the relationship between users, professionals, and decision makers.
Topics: Humans; COVID-19; Pandemics; Cooperative Behavior; Delivery of Health Care
PubMed: 38906819
DOI: No ID Found -
Communications Biology Jun 2024Human language relies on the correct processing of syntactic information, as it is essential for successful communication between speakers. As an abstract level of...
Human language relies on the correct processing of syntactic information, as it is essential for successful communication between speakers. As an abstract level of language, syntax has often been studied separately from the physical form of the speech signal, thus often masking the interactions that can promote better syntactic processing in the human brain. However, behavioral and neural evidence from adults suggests the idea that prosody and syntax interact, and studies in infants support the notion that prosody assists language learning. Here we analyze a MEG dataset to investigate how acoustic cues, specifically prosody, interact with syntactic representations in the brains of native English speakers. More specifically, to examine whether prosody enhances the cortical encoding of syntactic representations, we decode syntactic phrase boundaries directly from brain activity, and evaluate possible modulations of this decoding by the prosodic boundaries. Our findings demonstrate that the presence of prosodic boundaries improves the neural representation of phrase boundaries, indicating the facilitative role of prosodic cues in processing abstract linguistic features. This work has implications for interactive models of how the brain processes different linguistic features. Future research is needed to establish the neural underpinnings of prosody-syntax interactions in languages with different typological characteristics.
Topics: Humans; Speech Perception; Male; Female; Adult; Language; Brain; Speech; Young Adult; Magnetoencephalography; Linguistics; Cues
PubMed: 38902370
DOI: 10.1038/s42003-024-06444-7 -
Zeitschrift Fur Evidenz, Fortbildung... Jun 2024In Germany, an estimated number of 70,000 people diagnosed with long COVID or post-COVID syndrome (PCS) had received inpatient medical rehabilitation by the end of 2022....
["The idea of rehabilitation will have to be completely rethought for this illness" - Qualitative results of an online survey on patients' experiences with inpatient rehabilitation for post COVID-19 condition (long COVID)].
BACKGROUND
In Germany, an estimated number of 70,000 people diagnosed with long COVID or post-COVID syndrome (PCS) had received inpatient medical rehabilitation by the end of 2022. Due to the heterogeneity of the clinical picture, the variability of interventions and inconsistent endpoints, previous studies on the effectiveness of rehabilitation are of limited value. It therefore remains unclear whether and to what extent rehabilitation measures established for, e.g., pulmonary, cardiovascular, or neurological diseases are suitable for patients with PCS.
OBJECTIVES
To identify the experiences and perspectives of those affected by PCS, in relation to inpatient rehabilitation and to describe patients' values and wishes with respect to evidence-based medicine.
METHODS
In January/February 2023, the German initiative "Long COVID Deutschland" conducted a retrospective online survey (39 closed items, two open answer fields) among adult PCS sufferers on their experiences with inpatient rehabilitation. Recruitment was carried out via social media and websites of patient initiatives. The open answers were analyzed using a structuring and summarizing qualitative content analysis according to Mayring, supplemented by descriptive representations of the distribution of standardized information.
RESULTS
Of 1,191 participants in the survey, 733 used open response formats to additionally explain their experiences and the effects of individual measures on their general condition. 366 (50%) reported that their state of health deteriorated, mainly because of strength or endurance training and too extensive treatment plans. The presence of a post-exertional malaise (PEM) or its insufficient consideration during rehabilitation was described as the main barrier. Recognition and acceptance of individual performance limits, flexible and coordinated individual treatment plans tailored to the patient's limitations, and support in coping with the disease were described as supportive factors. From the participants' perspective, learning strategies to avoid deterioration in their state of health due to overexertion, the so-called pacing, should be the core treatment goal for patients affected by PEM.
DISCUSSION
The results are not statistically representative but ensure systematic insights into the subjective perspectives of those affected, the consideration of which represents one of the three principles of evidence-based medicine. The results show that for PCS patients with PEM, even minimum requirements for rehabilitation measures can lead to overexertion and aggravation.
CONCLUSIONS
Common subject-specific rehabilitation concepts appear to be only partially suitable for dealing with the symptoms and the heterogeneity of the disease. Aims and interventions should be individually adapted, and the focus should be on pacing, disease coping and management. A reliable test for PEM and rehabilitation ability before the start of rehabilitation is needed to ensure safety for those affected. To adequately classify studies on the effectiveness of rehabilitation, risks and side effects should be disclosed.
PubMed: 38890022
DOI: 10.1016/j.zefq.2024.05.007 -
Journal of Experimental Child Psychology Jun 2024In the current event-related potential (ERP) study, we assessed 4-year-olds' ability to extend verbs to new action events on the basis of abstract similarities....
In the current event-related potential (ERP) study, we assessed 4-year-olds' ability to extend verbs to new action events on the basis of abstract similarities. Participants were presented with images of actions (e.g., peeling an orange) while hearing sentences containing a conventional verb (e.g., peeling), a verb sharing an abstract relation (i.e., an analogical verb, e.g., undressing), a verb sharing an object type (i.e., an object-related verb, e.g., pressing) with the action, or a pseudoverb (e.g., kebraying). The amplitude of the N400 gradually increased as a function of verb type-from conventional verbs to analogical verbs to object-related verbs to pseudoverbs. These findings suggest that accessing the meaning of a verb is easier when it shares abstract relations with the expected verb. Our results illustrate that measuring brain signals in response to analogical word extensions provides a useful tool to investigate preschools' analogical abilities.
PubMed: 38879929
DOI: 10.1016/j.jecp.2024.105984 -
Assistenza Infermieristica E Ricerca :... 2024. Proposal for informed consent for the 'differentiated autonomy' experiment. Differentiated regional autonomy means the power given to the 15 ordinary Regions - the 5...
. Proposal for informed consent for the 'differentiated autonomy' experiment. Differentiated regional autonomy means the power given to the 15 ordinary Regions - the 5 special Regions are excluded - to obtain regulatory and management competences in areas that are today regulated and administered by the State. Although differentiated autonomy is bad for the entire country - which risks losing its ability to implement economic, social, environmental, and cultural policies at a national level - there is no doubt that the Southern Regions will pay the heaviest price for its implementation, since they are already far behind and penalised compared to the North, with a worsening of all the health parameters, such as: reduction in life expectancy, increase in avoidable mortality, increase in mortality, increase in trips of hope to seek treatment in the North, and more. But the North will also have its problems, with poorer areas even within the richest regions and in the urban peripheries. And if the idea of solidarity, contained in the Constitution, is rejected, it is easy to move from competition to conflict, not only between North and South but also within the Regions.
Topics: Informed Consent; Humans; Italy; Personal Autonomy
PubMed: 38873718
DOI: 10.1702/4280.42615 -
Revue Medicale de Liege Jun 2024It's easy to imagine using medicines to treat or even cure an illness. For most people, however, the idea of taking one or more medicines to prevent or delay the onset... (Review)
Review
It's easy to imagine using medicines to treat or even cure an illness. For most people, however, the idea of taking one or more medicines to prevent or delay the onset of an illness or its complications seems less obvious. However, there is indeed a place for using medicines in the field of prevention. Knowing the definition of a medicine means you can immediately understand the role it can play in the field of prevention. What's more, the use of medicines should be based not only on evidence-based medicine, but also on an approach that integrates a collegial discussion with the patient, which will make it possible to discuss the expected benefits of such an approach, as well as explaining any possible side-effects. Only in this way can we expect better compliance of a person still without a disease. This article briefly summarizes the role that medicines can play in a prevention strategy.
Topics: Humans; Pharmaceutical Preparations; Primary Prevention
PubMed: 38869112
DOI: No ID Found -
Beijing Da Xue Xue Bao. Yi Xue Ban =... Jun 2024To describe the current distribution of daily physical activity time among elementary and junior high school students in Beijing, and to analyze the influencing factors...
OBJECTIVE
To describe the current distribution of daily physical activity time among elementary and junior high school students in Beijing, and to analyze the influencing factors and pathways at the individual, family, school and community levels.
METHODS
Data were drawn upon from a cross-sectional investigation in Beijing in 2023, and a total of 3 157 elementary and junior high school students were included in the final analysis. Questionnaire was used to collect data on basic characteristics, overall and in-school physical activity time per day, the number of sports mastered, perceived physical activity benefits and barriers scales, perceived family, school, and community physical activity supportive environment scales. Log-binomial regression model was used to analyze the associations between physical activity time and influencing factors, and structural equation modeling was used for the path analysis of the influencing factors.
RESULTS
The reported rates of ≥2 hours of overall physical activity per day and ≥1 hour of physical activity in school per day among elementary and junior high school students in Beijing in 2023 were 33.1% and 64.8%, respectively. The associations between the number of sports mastered by students and the reported rate of ≥2 hours of overall physical activity per day showed a typical dose-response relationship (-<0.001). The perceived physical activity benefits-to-barriers ratio (=1.24, 95%: 1.20-1.28), scores of perceived family, school, and community physical acti-vity supportive environment scales were all positively associated with the reported rate of ≥2 hours of overall physical activity per day (=1.51, 95%: 1.38-1.66; =1.50, 95%: 1.37-1.64; =1.21, 95%: 1.16-1.27). The structural equation modeling showed that the number of sports mastered by the students (=0.11, <0.001), perceived physical activity benefits-to-barriers ratios (=0.15, <0.001), and scores of supportive environment scales consisting of family, school, and community (=0.13, <0.001) were associated with the reported rates of ≥2 hours of overall physical activity per day directly. In addition, the scores of supportive environment scales could indirectly influence the reported rates of ≥2 hours of overall physical activity per day by influencing the number of sports mastered by the students (=0.21, <0.001) and the perceived physical activity benefits-to-barriers ratio (=0.56, <0.001), while the number of sports mastered by the students could indirectly influence the reported rates of ≥2 hours of overall physical activity per day by influencing the perceived physical activity benefits-to-barriers ratios (=0.05, =0.003). The influencing factors and pathways of the reported rates of ≥1 hour of physical activity in school per day were similar with those of the reported rates of ≥2 hours of overall physical activity per day described above.
CONCLUSION
The daily physical activity time among elementary and junior high school students in Beijing in 2023 fell short of meeting the national requirement. There was a need to build a supportive environment consisting of family, school, and community for physical activity, to promote the students ' sports skills, to establish the idea of independent physical activity, and to ensure that primary and secondary school students were given one hour of physical activity time every day, both inside and outside the school.
Topics: Humans; Students; Adolescent; Cross-Sectional Studies; Beijing; Schools; Exercise; Male; Female; Surveys and Questionnaires; Child; Sports; China
PubMed: 38864124
DOI: 10.19723/j.issn.1671-167X.2024.03.005 -
Journal of Diabetes Science and... Jun 2024The use of insulin pumps (continuous subcutaneous insulin infusion [CSII]) in individuals living with type 1 diabetes (T1D) improves disease control. However, adverse...
Insulin Pump Therapy and Adverse Skin Reactions With Focus on Allergic Contact Dermatitis in Individuals Living With Diabetes Mellitus: A Systematic Review and Clinical-Based Update.
BACKGROUND
The use of insulin pumps (continuous subcutaneous insulin infusion [CSII]) in individuals living with type 1 diabetes (T1D) improves disease control. However, adverse skin reactions may hamper compliance. We aimed to assess the relationship of insulin pumps, particularly that of infusion set therapy, used in children and adults with T1D and dermatitis including allergic contact dermatitis (ACD).
METHODS
A systematic search of PubMed, and EMBASE, of full-text studies reporting dermatitis in persons with diabetes using a CSII was conducted from 2020 to 2023. The Newcastle-Ottawa Scale was used to assess study quality. The inventory performed at the Department of Occupational and Environmental Dermatology, Malmö, Sweden (YMDA) was also performed highlighting the diagnostic process.
RESULTS
Among the 391 screened abstracts, 21 studies fulfilled the inclusion criteria. Seven studies included data on children only, four studies were on adults, and nine studies reported data on both children and adults. Participants were exposed to a broad range of pumps. Dermatitis was rarely specified. Up to 60% of those referred to a university hospital due to skin reactions possibly related to insulin pumps had an ACD.
CONCLUSIONS
The review and our findings indicate that there is not sufficient focus on contact allergy in the primary toxicological evaluations of substances used also for insulin pump therapy products and that possible adverse skin reactions are not correctly followed up in the clinical setting.
PubMed: 38853748
DOI: 10.1177/19322968241252613 -
Sante Publique (Vandoeuvre-les-Nancy,... 2024At the last congress of the Association Dentaire Française (ADF) (French Dental Association), the minister of health and prevention, François Braun, stressed...
At the last congress of the Association Dentaire Française (ADF) (French Dental Association), the minister of health and prevention, François Braun, stressed the importance he attached to oral health prevention. He also emphasized his desire to roll out and extend prevention campaigns, targeting young people in particular. With this in mind, we are working to lay the political foundations for the mass, free distribution of single-use toothbrushes with fluoride toothpaste in schools. This oral health promotion campaign would aim to debunk any myths or misinformation spread by manufacturers and to encourage a profound cultural change in the way children and their parents learn about oral hygiene. The "cavity-score," which is still under development, could help to reinforce the idea, both among the general public and among healthcare professionals, that oral health is not isolated from the rest of the body, and that this health issue cannot be dealt with in isolation, but in conjunction with all healthcare professionals.
Topics: Humans; Oral Health; Child; Health Promotion; France; Oral Hygiene
PubMed: 38834529
DOI: 10.3917/spub.242.0091